FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_30484-1.34200000035803.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_30484-1.34200000035803.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8534077
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC46750.083.296039
CGGGAGA62600.078.668444
AGAGCGG53500.075.44958
ATTCGGA11800.074.390632
GATCGGG83600.073.1701351
ATCGGGA85100.069.696192
AAGCGGC15500.068.545529
GAGAGCG72150.068.110927
CGGAAAG23300.067.395645
GATCCGG13150.065.6799551
TTCGGAG12150.065.6013953
TCGGGAA19750.059.334863
AAAGCGG18950.059.322548
GAACCGG5900.058.7162171
GTTCGGA11650.058.242322
ACCGGAA5900.056.2893333
TCGGAGA21700.054.4398774
GGATCGG31650.052.7783971
TCGGAAA23250.052.4430164
ATCCGGA15900.051.6270752