FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_30423-1.34200000035748.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_30423-1.34200000035748.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2676500
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA313271.1704464786101252No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC243460.9096207733980946No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC121720.4547730244722586No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT118490.44270502521950306No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT117610.439417149262096No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT98820.3692135251260975Illumina Single End PCR Primer 1 (97% over 34bp)
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT59870.22368765178404632No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC52900.19764617971231085No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT51230.19140668783859519No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC35930.13424248085185878No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG33880.12658322436017186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC29850.087.5593959
ATCGGGA47300.086.569952
ATTCGGA7500.086.0389562
CGGGAGA39850.084.8895654
AAGCGGC9850.081.38539
AGAGCGG33000.081.216878
GTTCGGA5000.079.7125552
TCGGGAG45550.078.84983
ATCCGGA8250.077.0669862
CGGAGAG14650.076.105355
CGGAAAG18100.073.656775
TCGGAGA10750.072.8234
TCGGAAA14050.070.239294
TTGGGAG23350.069.49163
AAGCGTC3900.069.327549
GAGAGCG45100.069.004877
CCGGAGA7400.067.321024
TCGGGAA12450.066.690163
GGATCGG17750.066.5720141
AATCGGA7050.065.9560242
Produced by FastQC (version 0.11.2)