FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_30245-1.3420000003577e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_30245-1.3420000003577e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2256485
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC26620.11797109220757063No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC26460.11726202478633806No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG26180.11602115679918103No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG25100.1112349517058611No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA22690.10055462367354537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA7950.071.594912
CGGGAGA7750.068.540264
GAGCGGC5550.066.632549
AGAGCGG5950.062.9554678
CGGAAAG4050.060.89315
GAGAGCG8200.058.40897
GATCGGG10200.057.6696661
TCGGGAG9150.055.462793
CCGAGCG352.8065796E-454.2147981
GATCCGG2300.053.6255071
TCGGAAA4200.050.8150864
TTGTGTT281400.048.7361
GTTCGGA2200.043.1196632
ATCCGGA2750.043.119662
CGAGCGG459.6955325E-442.1614462
AGCGGCG5050.041.31463610-11
GAGCGTC20050.040.9087759
AGAGCGT20600.040.5106128
GGTTCGG2850.039.9477461
TTCGGAG2300.039.1800353
Produced by FastQC (version 0.11.2)