##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_30245-1.3420000003577e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2256485 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.93395923305495 33.0 33.0 33.0 33.0 33.0 2 31.860407669450495 33.0 33.0 33.0 33.0 33.0 3 31.813595481467857 33.0 33.0 33.0 33.0 33.0 4 35.51059235935537 37.0 37.0 37.0 33.0 37.0 5 35.48492190287106 37.0 37.0 37.0 33.0 37.0 6 35.56728584502002 37.0 37.0 37.0 33.0 37.0 7 35.494976478904135 37.0 37.0 37.0 33.0 37.0 8 35.527373326213116 37.0 37.0 37.0 33.0 37.0 9 35.367011524561434 37.0 37.0 37.0 33.0 37.0 10-11 35.31491346053707 37.0 37.0 37.0 33.0 37.0 12-13 35.32243156945426 37.0 37.0 37.0 33.0 37.0 14-15 37.13034897196303 40.0 37.0 40.0 33.0 40.0 16-17 37.100252162101675 40.0 37.0 40.0 33.0 40.0 18-19 37.12825101873046 40.0 37.0 40.0 33.0 40.0 20-21 37.11832584750175 40.0 37.0 40.0 33.0 40.0 22-23 37.23695659399464 40.0 37.0 40.0 33.0 40.0 24-25 37.00068292056008 40.0 37.0 40.0 33.0 40.0 26-27 36.91582350425551 40.0 37.0 40.0 33.0 40.0 28-29 36.8016490249215 40.0 37.0 40.0 33.0 40.0 30-31 36.83020228364026 40.0 37.0 40.0 33.0 40.0 32-33 36.74525777924515 40.0 37.0 40.0 33.0 40.0 34-35 36.691215540985205 40.0 37.0 40.0 33.0 40.0 36-37 36.57364307761851 40.0 37.0 40.0 33.0 40.0 38-39 36.57153005670324 40.0 37.0 40.0 33.0 40.0 40-41 36.46342807508138 40.0 37.0 40.0 33.0 40.0 42-43 36.346081848538766 40.0 37.0 40.0 33.0 40.0 44-45 36.255030279394724 40.0 37.0 40.0 33.0 40.0 46-47 36.19438994719664 40.0 37.0 40.0 33.0 40.0 48-49 36.09616372366756 40.0 37.0 40.0 33.0 40.0 50-51 35.003954380374786 37.0 35.0 38.5 27.0 38.5 52-53 35.40689833967431 37.0 37.0 38.5 30.0 40.0 54-55 36.17877894158393 38.5 37.0 40.0 33.0 40.0 56-57 36.25520599516504 40.0 37.0 40.0 33.0 40.0 58-59 36.07379508394693 37.0 37.0 40.0 33.0 40.0 60-61 35.86462506952185 37.0 37.0 40.0 27.0 40.0 62-63 35.6750696326366 37.0 37.0 40.0 27.0 40.0 64-65 35.45920003899871 37.0 37.0 40.0 27.0 40.0 66-67 35.2490171660791 37.0 37.0 40.0 27.0 40.0 68-69 35.059557453295724 37.0 37.0 40.0 27.0 40.0 70-71 34.7609195274952 37.0 33.0 37.0 27.0 40.0 72-73 34.56391112726209 37.0 33.0 37.0 27.0 40.0 74-75 34.36072253970224 37.0 33.0 37.0 27.0 40.0 76-77 34.1067950817311 37.0 33.0 37.0 27.0 38.5 78-79 33.82756122907974 37.0 33.0 37.0 27.0 37.0 80-81 33.5626826679548 37.0 33.0 37.0 27.0 37.0 82-83 33.304226706581254 37.0 33.0 37.0 27.0 37.0 84-85 33.14035679386302 37.0 33.0 37.0 27.0 37.0 86-87 32.96605273245778 37.0 33.0 37.0 27.0 37.0 88-89 32.74015360173013 37.0 33.0 37.0 27.0 37.0 90-91 32.66020359098332 37.0 33.0 37.0 24.5 37.0 92-93 32.51255049335582 37.0 33.0 37.0 22.0 37.0 94-95 32.38219731130497 35.0 33.0 37.0 22.0 37.0 96-97 32.248995007722186 33.0 33.0 37.0 22.0 37.0 98-99 32.11604220723825 33.0 33.0 37.0 22.0 37.0 100-101 30.916540548685234 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.23562249225967946 2306 2 0.23651130540690346 2306 3 0.16556096313299662 2306 4 0.2571001460154463 2306 5 0.25109514287127865 2306 6 0.28333906236425577 2306 7 0.3576298467918235 2306 8 0.2240513453496078 2306 9 0.28194741506260357 2306 10-11 0.29232237643858383 2306 12-13 0.2288629017404773 2306 14-15 0.41119679520435426 2306 16-17 0.36933451851982824 2306 18-19 0.3641840618903842 2306 20-21 0.3313343159010529 2306 22-23 0.37730312835468993 2306 24-25 0.4599142453679903 2306 26-27 0.3865652914175328 2306 28-29 0.22647263576998 2306 30-31 0.25857369649792616 2306 32-33 0.2619787048254736 2306 34-35 0.2822790854566506 2306 36-37 0.3036387832512446 2306 38-39 0.29288913636293756 2306 40-41 0.2747258394680756 2306 42-43 0.26644467456623033 2306 44-45 0.38558138849959533 2306 46-47 0.298017240844743 2306 48-49 0.3147373576947956 2306 50-51 0.3821784510946813 2306 52-53 0.30297754760533024 2306 54-55 0.36439007937395473 2306 56-57 0.5293811371458972 2306 58-59 0.47962848640280953 2306 60-61 0.4277373714873889 2306 62-63 0.3129634565926196 2306 64-65 0.3211146444549584 2306 66-67 0.29688652381772584 2306 68-69 0.2515422039044495 2306 70-71 0.2956627896978006 2306 72-73 0.34857792253153264 2306 74-75 0.530988679898833 2306 76-77 0.3446842127557872 2306 78-79 0.25132535037778325 2306 80-81 0.22733552409038538 2306 82-83 0.2563435942922183 2306 84-85 0.24486038557908074 2306 86-87 0.42126757120924907 2306 88-89 0.49267313944334745 2306 90-91 0.21602532797127338 2306 92-93 0.12181450443392094 2306 94-95 -0.008194612163549664 2306 96-97 0.2336019355478598 2306 98-99 0.27883295352107496 2306 100-101 -3.3262070227380036E-4 2307 1 0.2652057015399478 2307 2 0.21732443187486084 2307 3 0.2499473691228289 2307 4 0.41935121287177424 2307 5 0.3605388889429335 2307 6 0.24095702304219202 2307 7 0.4311905556034503 2307 8 0.28747140555668693 2307 9 0.3424494715866899 2307 10-11 0.25156887814225115 2307 12-13 0.2856268260074657 2307 14-15 0.4851234605477899 2307 16-17 0.4385199447661492 2307 18-19 0.40082313364267463 2307 20-21 0.2489937516525984 2307 22-23 0.3974965546451159 2307 24-25 0.43225213558378783 2307 26-27 0.38966765173378803 2307 28-29 0.250908676768141 2307 30-31 0.2822661083099831 2307 32-33 0.25882362195802955 2307 34-35 0.43472375779116845 2307 36-37 0.299277973543127 2307 38-39 0.03944563012719726 2307 40-41 0.1635232966559954 2307 42-43 0.15079207276001938 2307 44-45 0.19546007838899726 2307 46-47 0.27143335892120035 2307 48-49 0.12920704464000465 2307 50-51 0.170390584586805 2307 52-53 0.24958545447179858 2307 54-55 0.0792155229325644 2307 56-57 0.11579064181579923 2307 58-59 0.2037703148898018 2307 60-61 0.223007779594397 2307 62-63 0.177353129313083 2307 64-65 0.2007211481434652 2307 66-67 0.14315032127491634 2307 68-69 0.0019884503344727023 2307 70-71 0.0431841406080693 2307 72-73 -0.08519049245627741 2307 74-75 0.09844356593542614 2307 76-77 0.06790253511731947 2307 78-79 0.08525310463142688 2307 80-81 0.19198970550558414 2307 82-83 0.24887077569638905 2307 84-85 0.19425604041384048 2307 86-87 0.3065712117531021 2307 88-89 0.4840324991090128 2307 90-91 0.2525110563557078 2307 92-93 0.36860497605609766 2307 94-95 0.24986883684236716 2307 96-97 0.2819175017260349 2307 98-99 0.1785570732956856 2307 100-101 0.24549161540653586 2201 1 0.2655034469293227 2201 2 0.24766891248112088 2201 3 0.2274130547036961 2201 4 0.2516655298065089 2201 5 0.3231015825265686 2201 6 0.33011948393240687 2201 7 0.27861127475191694 2201 8 0.2401878654721017 2201 9 0.2802079106540276 2201 10-11 0.31716425100201917 2201 12-13 0.3087389433604173 2201 14-15 0.5020065085172263 2201 16-17 0.4114128311079881 2201 18-19 0.36729975424233174 2201 20-21 0.30850892782353156 2201 22-23 0.2956592788876833 2201 24-25 0.44771475134091077 2201 26-27 0.5318115954156895 2201 28-29 0.5249341747792471 2201 30-31 0.5333121478049776 2201 32-33 0.4660799620677665 2201 34-35 0.47276834688288005 2201 36-37 0.4374608226225689 2201 38-39 0.4696282669525118 2201 40-41 0.4987295762339983 2201 42-43 0.5190873968799465 2201 44-45 0.5621079762868888 2201 46-47 0.5069656844404307 2201 48-49 0.4504527012914181 2201 50-51 0.44192700975542465 2201 52-53 0.45484454209345415 2201 54-55 0.5982103030530013 2201 56-57 0.5451714296717185 2201 58-59 0.516027598351279 2201 60-61 0.5984824034329037 2201 62-63 0.4995269064934007 2201 64-65 0.44192635858951235 2201 66-67 0.4150930755475173 2201 68-69 0.42870820018904254 2201 70-71 0.4185737645251777 2201 72-73 0.2447787110404107 2201 74-75 0.48815543094179503 2201 76-77 0.43081780640955714 2201 78-79 0.41162872763663216 2201 80-81 0.41727438154288166 2201 82-83 0.515425615876886 2201 84-85 0.5907983615307657 2201 86-87 0.43899175402311386 2201 88-89 0.5582619969314422 2201 90-91 0.5656856511075361 2201 92-93 0.5450952752558251 2201 94-95 0.5257088129441243 2201 96-97 0.41597220433440185 2201 98-99 0.4469185303024332 2201 100-101 0.5311060911388132 1101 1 0.1768198490950752 1101 2 0.1821561901364639 1101 3 0.1386740690967443 1101 4 0.27037526030450465 1101 5 0.22982047565213293 1101 6 0.2382081717410074 1101 7 0.2760961516226317 1101 8 0.25456795645057184 1101 9 0.3101053234296245 1101 10-11 0.27484507750419596 1101 12-13 0.28948926305475453 1101 14-15 0.4600439545384418 1101 16-17 0.4731821821705964 1101 18-19 0.45841733697741205 1101 20-21 0.3935485932425138 1101 22-23 0.4771097842444263 1101 24-25 0.4673144111109906 1101 26-27 0.34689805864403667 1101 28-29 0.2630897749041452 1101 30-31 0.276781573106625 1101 32-33 0.415199652777261 1101 34-35 0.3792401004144139 1101 36-37 0.36241314379132206 1101 38-39 0.36804935913460923 1101 40-41 0.42785444294705144 1101 42-43 0.41030645908397645 1101 44-45 0.4237758802710587 1101 46-47 0.41425950912485376 1101 48-49 0.4021095406772446 1101 50-51 0.3726976382997549 1101 52-53 0.3808048559445396 1101 54-55 0.211624379450555 1101 56-57 0.38567780347634795 1101 58-59 0.4479591027868324 1101 60-61 0.6116705236301954 1101 62-63 0.5054815779175854 1101 64-65 0.5288075864748905 1101 66-67 0.4609335833788961 1101 68-69 0.26268351343767193 1101 70-71 0.400382444145464 1101 72-73 0.40389498779011035 1101 74-75 0.4047793421347805 1101 76-77 0.4227551605712776 1101 78-79 0.33247603747518184 1101 80-81 0.4454790662471737 1101 82-83 0.5963910409505004 1101 84-85 0.5388412297234169 1101 86-87 0.5231429477429828 1101 88-89 0.6812494125703594 1101 90-91 0.7550611426335294 1101 92-93 0.5855272156676463 1101 94-95 0.5472413764198123 1101 96-97 0.47211305641211965 1101 98-99 0.6199251535139894 1101 100-101 0.6150904737303406 2304 1 0.08012386137486516 2304 2 0.09912550641054807 2304 3 0.051146903672517396 2304 4 0.14968605073688224 2304 5 0.13146028965746837 2304 6 0.11207183108939489 2304 7 0.22818331021477434 2304 8 0.034658931124639025 2304 9 0.06776333236977194 2304 10-11 0.17481900768652991 2304 12-13 0.13650999979679312 2304 14-15 0.3056881599596082 2304 16-17 0.23511923704531057 2304 18-19 0.1640792631324075 2304 20-21 0.13776322874825553 2304 22-23 0.19499867962579742 2304 24-25 0.17983539619051925 2304 26-27 0.26058785927735784 2304 28-29 0.2492112490174918 2304 30-31 0.09760045754317304 2304 32-33 0.17187554875869182 2304 34-35 0.08301786420837942 2304 36-37 0.1291593496215242 2304 38-39 -0.06261054436212277 2304 40-41 0.049627741161039296 2304 42-43 -0.036339748768881464 2304 44-45 0.2345208028369541 2304 46-47 -0.05171881696811198 2304 48-49 0.07021042188202387 2304 50-51 -0.013747798246079412 2304 52-53 0.09918256040720763 2304 54-55 0.20444506630035875 2304 56-57 0.0704771333022407 2304 58-59 0.23078825109038092 2304 60-61 0.12562855122374827 2304 62-63 0.007944726207654185 2304 64-65 0.20508815389795387 2304 66-67 0.23031430160236965 2304 68-69 0.18054336119892156 2304 70-71 0.049511071726875855 2304 72-73 0.04613871136913872 2304 74-75 0.10232563291663865 2304 76-77 0.18684690835028306 2304 78-79 0.17923776158908566 2304 80-81 0.1737248147318624 2304 82-83 0.31030260358885897 2304 84-85 0.3192875742180803 2304 86-87 0.19187253855921682 2304 88-89 0.28318801814975103 2304 90-91 0.37762383942132516 2304 92-93 0.285482436261006 2304 94-95 0.2411075528540394 2304 96-97 0.28335015915642003 2304 98-99 0.18626637821109426 2304 100-101 0.08869815443799922 2202 1 0.26491938875363985 2202 2 0.17914639369682206 2202 3 0.14661601579147643 2202 4 0.22003195105251194 2202 5 0.22826913167317286 2202 6 0.2617425438277152 2202 7 0.32683995125284326 2202 8 0.23294147602675963 2202 9 0.24129162042883934 2202 10-11 0.28862472769809955 2202 12-13 0.333272873653506 2202 14-15 0.415601203525533 2202 16-17 0.3703946578908628 2202 18-19 0.3354948808144229 2202 20-21 0.2817917532005936 2202 22-23 0.22573560163416317 2202 24-25 0.37040491748657445 2202 26-27 0.45053161884775506 2202 28-29 0.37004601465548603 2202 30-31 0.4529166852105604 2202 32-33 0.48885372698813256 2202 34-35 0.3609326840000975 2202 36-37 0.4384965815117283 2202 38-39 0.42675679868837335 2202 40-41 0.3775581356908262 2202 42-43 0.36042871989666736 2202 44-45 0.45094136512027916 2202 46-47 0.39407479406263235 2202 48-49 0.36831872125168275 2202 50-51 0.31318838363460344 2202 52-53 0.30831248194882477 2202 54-55 0.4990513177683624 2202 56-57 0.49586355313871167 2202 58-59 0.40737301849251395 2202 60-61 0.5064906255772499 2202 62-63 0.5397663354762869 2202 64-65 0.5980231704454866 2202 66-67 0.5557366088612028 2202 68-69 0.5534890330850644 2202 70-71 0.5062551349290629 2202 72-73 0.6541661531503635 2202 74-75 0.6078707941126495 2202 76-77 0.7290229462377269 2202 78-79 0.57965369278201 2202 80-81 0.6238407936066679 2202 82-83 0.49932246364807753 2202 84-85 0.5799640823718804 2202 86-87 0.6173254912573825 2202 88-89 0.7733071321336453 2202 90-91 0.7557845283791877 2202 92-93 0.7139313838825032 2202 94-95 0.6313454683190045 2202 96-97 0.5621026881612181 2202 98-99 0.7539701174587279 2202 100-101 0.6346199420348633 1102 1 0.18602442750573545 1102 2 0.17357027047364326 1102 3 0.19832900868094328 1102 4 0.28444792723319523 1102 5 0.31566539554002304 1102 6 0.29305436925718453 1102 7 0.303386280740348 1102 8 0.1014194033282152 1102 9 0.2531033381229122 1102 10-11 0.3029078302164976 1102 12-13 0.2757617573188682 1102 14-15 0.29731262935460734 1102 16-17 0.30582687273484055 1102 18-19 0.3078378972750855 1102 20-21 0.44026236755356507 1102 22-23 0.43029901207992793 1102 24-25 0.4373804256385583 1102 26-27 0.44326437628639326 1102 28-29 0.3539216161781411 1102 30-31 0.3264189998893485 1102 32-33 0.4205968204959305 1102 34-35 0.3571178713617513 1102 36-37 0.45202390386258173 1102 38-39 0.37683123405692953 1102 40-41 0.37490719186341437 1102 42-43 0.4219260460998697 1102 44-45 0.6023291929786936 1102 46-47 0.5257535728002338 1102 48-49 0.4391694391612191 1102 50-51 0.44686516437020884 1102 52-53 0.40060907424542336 1102 54-55 0.4633468261815139 1102 56-57 0.46309566742964847 1102 58-59 0.5746208033873543 1102 60-61 0.5771846374477221 1102 62-63 0.6574280854909773 1102 64-65 0.5366515355444434 1102 66-67 0.5238404004944073 1102 68-69 0.5394915926170327 1102 70-71 0.5582293045502951 1102 72-73 0.573725352121329 1102 74-75 0.6583138151439059 1102 76-77 0.5191886628740363 1102 78-79 0.5480268229198444 1102 80-81 0.6307113740066725 1102 82-83 0.522593310448336 1102 84-85 0.544416296824096 1102 86-87 0.5473350242080883 1102 88-89 0.7266528060675554 1102 90-91 0.6683866321577554 1102 92-93 0.6185463767327946 1102 94-95 0.614003333329812 1102 96-97 0.679439599615776 1102 98-99 0.7244212349686663 1102 100-101 0.6983650204858236 2305 1 0.25453594619058606 2305 2 0.21772895239164214 2305 3 0.21794179011070014 2305 4 0.3229757799084112 2305 5 0.2437746738391482 2305 6 0.44743103681274476 2305 7 0.2956214036638585 2305 8 0.3485508432731734 2305 9 0.4251760241448608 2305 10-11 0.33120095409098127 2305 12-13 0.3979086588225087 2305 14-15 0.3610942044441643 2305 16-17 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-0.8566921893453561 1213 90-91 -1.0374723966242527 1213 92-93 -0.9671660208352719 1213 94-95 -0.7759740163200703 1213 96-97 -0.8588521907985118 1213 98-99 -1.0593910210995006 1213 100-101 -0.8717474019613469 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1654.0 3 221.0 4 172.0 5 171.0 6 296.0 7 1882.0 8 6886.0 9 12016.0 10 14173.0 11 12745.0 12 9354.0 13 7072.0 14 5532.0 15 4641.0 16 4430.0 17 4398.0 18 4717.0 19 5238.0 20 6000.0 21 6626.0 22 7570.0 23 8326.0 24 9171.0 25 10637.0 26 12653.0 27 15035.0 28 18305.0 29 22632.0 30 28228.0 31 36930.0 32 50793.0 33 72538.0 34 111704.0 35 189732.0 36 386081.0 37 793648.0 38 373125.0 39 1153.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.32383664071328 24.922275349433235 26.22256643086005 14.531321578993438 2 29.53861464437563 23.501863756925225 34.68387217159892 12.275649427100225 3 38.028649416379395 20.042422525152844 26.63856559280949 15.290362465658273 4 28.295856637370164 19.547899094723387 36.355718680729105 15.800525587177342 5 30.929624212046413 18.420001750144255 35.12215251752032 15.528221520289016 6 23.721671548546496 18.978252313673437 41.78595955369224 15.514116584087825 7 31.905138339920946 18.90980148332371 32.20313540880223 16.981924767953103 8 27.78060510784562 28.180324447227367 27.899308562369274 16.139761882557742 9 30.362351110860498 26.85583344396281 25.63865765232568 17.143157792851014 10-11 24.246112361066963 29.211453462870985 29.920058565413733 16.62237561064832 12-13 24.960515982228916 26.158925031102058 29.908002738180272 18.97255624848875 14-15 23.236199996802778 26.903656199321833 31.030046768231706 18.830097035643682 16-17 22.401171628160817 28.103333660463186 32.057667516760226 17.437827194615767 18-19 21.819722597549763 28.332823440449324 32.243672258911324 17.603781703089595 20-21 21.646303287358098 29.092324346982036 31.679841142551645 17.581531223108218 22-23 22.157187870437426 28.82243705851294 31.270384115243672 17.749990955805963 24-25 21.537513833470285 29.321792065270603 31.22053117409123 17.92016292716788 26-27 21.148341718686538 29.841060300512147 31.068350540147765 17.942247440653553 28-29 21.178252842578697 29.488005876577212 30.976681000377955 18.357060280466136 30-31 21.411969862475548 29.212902927627454 30.881474594905917 18.49365261499108 32-33 20.428448622156164 29.260538653009572 32.275964910837686 18.035047813996584 34-35 20.157218255421093 30.206373690227622 31.75970588097996 17.876702173371328 36-37 20.426919194804277 30.3332502765657 31.304769955351613 17.93506057327841 38-39 20.65862778430195 29.36478363006319 31.74792332083687 18.228665264797993 40-41 20.956302839386055 29.655424502711675 30.706231245519827 18.682041412382446 42-43 20.853203059107393 29.245930561536245 31.391771976213267 18.509094403143095 44-45 20.349324568608417 29.555443096067037 31.655579574253874 18.439652761070676 46-47 20.84384679660844 29.6909247525532 30.937305322538784 18.527923128299577 48-49 20.421050759118764 30.054943833216225 30.667286294948994 18.856719112716018 50-51 20.41399075325313 29.4841695942694 30.777103937384737 19.324735715092732 52-53 21.12148801148894 29.252118206378825 30.33612217627151 19.290271605860728 54-55 21.47092911188485 29.380475726663818 30.063213677258304 19.08538148419302 56-57 21.662643640709124 29.30556262942871 29.996190116945392 19.03560361291677 58-59 21.334117001371197 29.26599836950793 30.48817804297365 18.911706586147222 60-61 20.36705311793077 29.56005377950403 30.3332897619759 19.739603340589298 62-63 20.833133307730055 29.506865767707158 30.031355124567057 19.628645799995734 64-65 20.25414476632124 29.556153493268088 30.485058570829093 19.704643169581576 66-67 20.131367248125343 29.12932798599413 30.437532203847965 20.301772562032557 68-69 19.824516943651577 29.372004517121493 31.2772650074248 19.526213531802135 70-71 19.485881350225412 30.809826326884167 30.425876394168743 19.27841592872168 72-73 19.56296286417909 31.113007761448802 29.831568559464433 19.492460814907673 74-75 19.87196740487402 30.66988077984177 29.928080291190472 19.530071524093735 76-77 19.926456958161282 30.474575773915603 30.133354552231605 19.465612715691506 78-79 19.869197861128974 30.610652256974813 30.1942938989453 19.325855982950912 80-81 19.43041430315598 31.134004942364278 30.065167536189612 19.37041321829013 82-83 19.278490204911307 30.76829177726618 30.056956618605447 19.896261399217067 84-85 19.39200965470597 30.33448406886804 30.46321644626157 19.810289830164418 86-87 19.556602951325893 30.380006332401262 30.698694209054622 19.364696507218227 88-89 19.898548597956296 30.907281911724162 29.876381811228804 19.31778767909074 90-91 20.056368520315228 30.535245328647807 29.706276888128713 19.702109262908245 92-93 19.662151125255438 30.71722324761176 30.014767831684498 19.60585779544831 94-95 19.235085387497097 31.071536130352207 30.02308190687445 19.670296575276243 96-97 19.519083199290993 31.393019546106494 29.699285882863812 19.388611371738705 98-99 20.05657333873609 30.713863868792174 29.31935729735389 19.91020549511785 100-101 20.02623443580956 30.623012269045258 28.9862058002559 20.364547494889287 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1212.0 1 773.5 2 283.0 3 229.5 4 242.0 5 304.0 6 359.0 7 440.0 8 597.5 9 781.0 10 931.0 11 999.5 12 1028.0 13 954.0 14 888.0 15 875.0 16 881.0 17 910.0 18 975.5 19 1137.0 20 1456.0 21 1975.0 22 2746.0 23 3967.5 24 5621.5 25 7890.0 26 11592.5 27 16919.5 28 22868.5 29 28569.5 30 34452.5 31 40248.0 32 47041.0 33 57047.0 34 69309.5 35 85255.0 36 105200.5 37 124757.0 38 144821.0 39 156927.0 40 157610.0 41 146768.5 42 129515.5 43 118826.0 44 107938.0 45 97926.5 46 94386.0 47 85900.5 48 69083.0 49 55598.5 50 45937.0 51 36913.0 52 29355.0 53 23413.0 54 19229.0 55 15162.5 56 11106.5 57 7901.5 58 5706.0 59 4175.5 60 3031.0 61 2155.0 62 1555.0 63 1101.5 64 767.5 65 551.0 66 407.5 67 291.5 68 219.5 69 176.0 70 139.0 71 119.5 72 90.5 73 83.0 74 76.5 75 55.5 76 42.5 77 36.0 78 33.5 79 31.5 80 30.5 81 28.5 82 27.5 83 20.5 84 16.0 85 13.5 86 10.0 87 11.5 88 12.5 89 9.5 90 6.0 91 6.0 92 4.5 93 1.5 94 1.5 95 2.5 96 3.0 97 2.5 98 2.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2620004121454386 2 0.25061101669189023 3 0.21498037877495305 4 0.21750643146309415 5 0.23230821388132428 6 0.2350115334247735 7 0.21205547566236865 8 0.20629430286485395 9 0.18710516577774725 10-11 0.17553850346889077 12-13 0.20494264309312937 14-15 0.2007103969226474 16-17 0.2027932824725181 18-19 0.19401857313476492 20-21 0.1951264909804408 22-23 0.1621326975362123 24-25 0.46858277365016826 26-27 0.32902944180883104 28-29 0.27456420051540337 30-31 0.2777328455540365 32-33 0.3235341692942785 34-35 0.31870364748713154 36-37 0.3110811727088813 38-39 0.3360093242365892 40-41 0.3407512126160821 42-43 0.37709091795425187 44-45 0.3445181332913802 46-47 0.3711746366583425 48-49 0.34677828569655905 50-51 0.36973434345896383 52-53 0.3572591885166531 54-55 0.33350542990536164 56-57 0.3715734870827858 58-59 0.3582563145777614 60-61 0.323556327651192 62-63 0.3001571027505168 64-65 0.3058296421203775 66-67 0.3165099701526932 68-69 0.3219609259534187 70-71 0.29747594156398116 72-73 0.3060069089756856 74-75 0.3023950967987822 76-77 0.3065830262554371 78-79 0.3093528208696269 80-81 0.3261266970531601 82-83 0.34372043244249356 84-85 0.3382916349986816 86-87 0.3427011480244717 88-89 0.33791494293115176 90-91 0.3103277885738217 92-93 0.29526010587262935 94-95 0.3052313664837125 96-97 0.29133807669893663 98-99 0.31090390585357314 100-101 0.33381564690215093 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2256485.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.69285354169935 #Duplication Level Percentage of deduplicated Percentage of total 1 93.27825043276395 64.07549195603809 2 3.465750997042219 4.761446513036393 3 0.8983060410452233 1.8512161593942984 4 0.4802895353901101 1.3196983484865465 5 0.3433396579816833 1.179249042039646 6 0.25770073796831 1.0621319430506977 7 0.17654475666660882 0.8489154179278017 8 0.1068114227327444 0.5869745134688757 9 0.1533602343902633 0.9481276908081936 >10 0.7063676435002427 9.290847888226939 >50 0.07127938729327472 3.410891804070492 >100 0.05842598677303901 8.359565347089882 >500 0.0028573994172873354 1.2929820643503953 >1k 7.157670352050488E-4 1.0124613120116215 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2662 0.11797109220757063 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2646 0.11726202478633806 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2618 0.11602115679918103 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2510 0.1112349517058611 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 2269 0.10055462367354537 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.533841350596171E-4 0.0 0.0 2 7.977008488866534E-4 0.0 0.0 3 7.977008488866534E-4 0.0 0.0 4 8.420175627136897E-4 0.0 0.0 5 9.749677041947986E-4 0.0 0.0 6 0.0011522345595029438 0.0 0.0 7 0.0014624515562921979 0.0 0.0 8 0.0015510849839462705 0.0 0.0 9 0.0016840351254273794 0.0 0.0 10-11 0.00239310254665996 0.0 0.0 12-13 0.0034567036785088314 0.0 0.0 14-15 0.004586779881098257 0.0 0.0 16-17 0.005916281295909346 0.0 4.43167138270363E-5 18-19 0.0073787328522015435 0.0 4.43167138270363E-5 20-21 0.008641759196272077 0.0 4.43167138270363E-5 22-23 0.010192844180218348 0.0 4.43167138270363E-5 24-25 0.012386521514656645 0.0 4.43167138270363E-5 26-27 0.015289266270327524 0.0 4.43167138270363E-5 28-29 0.0179482690999497 0.0 4.43167138270363E-5 30-31 0.02791952971103287 0.0 4.43167138270363E-5 32-33 0.03159781695867688 0.0 4.43167138270363E-5 34-35 0.03509883735101275 0.0 4.43167138270363E-5 36-37 0.03875496624174324 0.0 4.43167138270363E-5 38-39 0.04354117133506316 0.0 4.43167138270363E-5 40-41 0.04828305971455604 0.0 4.43167138270363E-5 42-43 0.0540885492258978 0.0 4.43167138270363E-5 44-45 0.06020425573402881 0.0 4.43167138270363E-5 46-47 0.066541545811295 0.0 4.43167138270363E-5 48-49 0.07212545175350157 0.0 4.43167138270363E-5 50-51 0.07821899990471906 0.0 4.43167138270363E-5 52-53 0.08519888233247729 0.0 4.43167138270363E-5 54-55 0.09297646560912215 0.0 4.43167138270363E-5 56-57 0.10117505766712387 0.0 4.43167138270363E-5 58-59 0.11045940921388797 0.0 4.43167138270363E-5 60-61 0.12089599532015502 0.0 4.43167138270363E-5 62-63 0.13292798312419538 0.0 6.647507074055445E-5 64-65 0.14535882135267905 0.0 1.329501414811089E-4 66-67 0.158742468928444 0.0 1.329501414811089E-4 68-69 0.17367720148815524 0.0 1.329501414811089E-4 70-71 0.1902294941025533 0.0 1.329501414811089E-4 72-73 0.20859877198385984 0.0 1.329501414811089E-4 74-75 0.22878503513207488 0.0 1.329501414811089E-4 76-77 0.2527603773125015 0.0 1.329501414811089E-4 78-79 0.28200940843834543 0.0 1.329501414811089E-4 80-81 0.3127430494773952 0.0 1.329501414811089E-4 82-83 0.3483958457512458 0.0 1.329501414811089E-4 84-85 0.38455828423410743 0.0 1.772668553081452E-4 86-87 0.4266813207267055 0.0 1.772668553081452E-4 88-89 0.47536323086570487 0.0 1.772668553081452E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 795 0.0 71.59491 2 CGGGAGA 775 0.0 68.54026 4 GAGCGGC 555 0.0 66.63254 9 AGAGCGG 595 0.0 62.955467 8 CGGAAAG 405 0.0 60.8931 5 GAGAGCG 820 0.0 58.4089 7 GATCGGG 1020 0.0 57.669666 1 TCGGGAG 915 0.0 55.46279 3 CCGAGCG 35 2.8065796E-4 54.214798 1 GATCCGG 230 0.0 53.625507 1 TCGGAAA 420 0.0 50.815086 4 TTGTGTT 28140 0.0 48.736 1 GTTCGGA 220 0.0 43.119663 2 ATCCGGA 275 0.0 43.11966 2 CGAGCGG 45 9.6955325E-4 42.161446 2 AGCGGCG 505 0.0 41.314636 10-11 GAGCGTC 2005 0.0 40.908775 9 AGAGCGT 2060 0.0 40.510612 8 GGTTCGG 285 0.0 39.947746 1 TTCGGAG 230 0.0 39.180035 3 >>END_MODULE