FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_30230-1.34200000035711.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_30230-1.34200000035711.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2236066
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA324401.4507621868048617No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC222420.9946933587827907No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT122910.5496707163384266No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT119940.5363884608057187No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC104650.468009441581778No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT58860.2632301551027563No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT58710.2625593341162559No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC53380.2387228283959418No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT38570.17249043632880245Illumina Single End PCR Primer 1 (96% over 32bp)
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC30610.13689220264518132No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT29560.13219645573967853No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC29400.1314809133540781No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC26700.11940613559707092No Hit
TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA26000.11627563766006907No Hit
GTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC24120.10786801462926408No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG23710.10603443726616298No Hit
AACTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAA23110.1033511533201614No Hit
GTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCT22860.10223311834266073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC9300.070.88279
ATTCGGA2700.065.014732
CGGGAGA12850.063.8686334
AGAGCGG10600.062.1895378
AAGCGGC3850.060.3592349
TCGGGAG14250.059.5926253
ATCCGGA2800.059.3039782
ATCGGGA17700.056.0204852
GGTTCGG3100.055.101491
GTTCGGA2400.053.373582
CGGACCG451.5272144E-552.714652
TTGTGTT293900.051.403891
GGATCGG7050.051.1501581
GATCCGG3350.050.9894331
TCGGGAA4150.048.0126383
TCGGAGA3900.047.4399954
TCGGAAA5400.043.9259224
AAGGGGC10300.043.281179
GATCGGG26100.041.994651
TGTGTTT534350.041.7788351
Produced by FastQC (version 0.11.2)