##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_30230-1.34200000035711.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2236066 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.836710991536027 33.0 33.0 33.0 27.0 33.0 2 31.68165832314431 33.0 33.0 33.0 27.0 33.0 3 31.627929139837555 33.0 33.0 33.0 27.0 33.0 4 35.28596070062333 37.0 37.0 37.0 33.0 37.0 5 35.27246109909099 37.0 37.0 37.0 33.0 37.0 6 35.44753643228778 37.0 37.0 37.0 33.0 37.0 7 35.300713842972435 37.0 37.0 37.0 33.0 37.0 8 35.32372568609335 37.0 37.0 37.0 33.0 37.0 9 35.08127711793838 37.0 37.0 37.0 33.0 37.0 10-11 35.02901613816408 37.0 37.0 37.0 33.0 37.0 12-13 35.014543622594324 37.0 37.0 37.0 33.0 37.0 14-15 36.70836616629384 40.0 37.0 40.0 33.0 40.0 16-17 36.597291180135116 40.0 37.0 40.0 33.0 40.0 18-19 36.685030763850435 40.0 37.0 40.0 33.0 40.0 20-21 36.65512735312822 40.0 37.0 40.0 33.0 40.0 22-23 36.85432362014359 40.0 37.0 40.0 33.0 40.0 24-25 36.53440797364657 40.0 37.0 40.0 33.0 40.0 26-27 36.37145594092482 40.0 37.0 40.0 33.0 40.0 28-29 36.24900919740294 40.0 37.0 40.0 33.0 40.0 30-31 36.294477220260944 40.0 37.0 40.0 33.0 40.0 32-33 36.25702953311754 40.0 37.0 40.0 33.0 40.0 34-35 36.16632179014394 40.0 37.0 40.0 33.0 40.0 36-37 35.96062973990929 38.5 37.0 40.0 30.0 40.0 38-39 35.94806660447411 37.0 37.0 40.0 27.0 40.0 40-41 35.798143927773154 37.0 37.0 40.0 27.0 40.0 42-43 35.648721907135126 37.0 37.0 40.0 27.0 40.0 44-45 35.503132510399965 37.0 37.0 40.0 27.0 40.0 46-47 35.39204097732357 37.0 37.0 40.0 27.0 40.0 48-49 35.25990109415375 37.0 37.0 40.0 27.0 40.0 50-51 34.10565900112071 37.0 35.0 38.5 24.5 38.5 52-53 34.54887802953938 37.0 35.0 38.5 27.0 40.0 54-55 35.27765191188453 37.0 37.0 40.0 27.0 40.0 56-57 35.31716505684537 37.0 37.0 40.0 27.0 40.0 58-59 35.057085747916204 37.0 37.0 40.0 27.0 40.0 60-61 34.83782746126456 37.0 33.0 40.0 27.0 40.0 62-63 34.61014411023646 37.0 33.0 40.0 27.0 40.0 64-65 34.353210951733985 37.0 33.0 37.0 27.0 40.0 66-67 34.07259647076607 37.0 33.0 37.0 27.0 40.0 68-69 33.89649030932003 37.0 33.0 37.0 27.0 40.0 70-71 33.57325141565589 37.0 33.0 37.0 27.0 37.0 72-73 33.42263130873597 37.0 33.0 37.0 27.0 37.0 74-75 33.28200240958898 37.0 33.0 37.0 27.0 37.0 76-77 32.99576980285913 37.0 33.0 37.0 27.0 37.0 78-79 32.797856145569945 37.0 33.0 37.0 22.0 37.0 80-81 32.61377526423638 37.0 33.0 37.0 22.0 37.0 82-83 32.23642705537314 35.0 33.0 37.0 22.0 37.0 84-85 32.12250264527076 33.0 33.0 37.0 22.0 37.0 86-87 31.992323795451476 33.0 33.0 37.0 22.0 37.0 88-89 31.84698126083935 33.0 33.0 37.0 22.0 37.0 90-91 31.836763091965977 33.0 33.0 37.0 22.0 37.0 92-93 31.660201443070108 33.0 33.0 37.0 15.0 37.0 94-95 31.443732653687324 33.0 33.0 37.0 15.0 37.0 96-97 31.296668792423837 33.0 33.0 37.0 15.0 37.0 98-99 31.169606800514835 33.0 33.0 37.0 15.0 37.0 100-101 29.82131743875181 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.2775273091785486 2306 2 0.2638206670501049 2306 3 0.21628687287644865 2306 4 0.31256382284969675 2306 5 0.3450766101330274 2306 6 0.2865212492828846 2306 7 0.3920442879007595 2306 8 0.2512631794778173 2306 9 0.4120145647830782 2306 10-11 0.4266537110893722 2306 12-13 0.38081876798895564 2306 14-15 0.5924577346791509 2306 16-17 0.5959519688914483 2306 18-19 0.5730960869650872 2306 20-21 0.5126170514580082 2306 22-23 0.5669661797441634 2306 24-25 0.6623182952597233 2306 26-27 0.5695587809939227 2306 28-29 0.49934883427836496 2306 30-31 0.4906087535900454 2306 32-33 0.4554106999057268 2306 34-35 0.25084551737632665 2306 36-37 0.4368270571442352 2306 38-39 0.44007984075251727 2306 40-41 0.4815337648728999 2306 42-43 0.4910257766357873 2306 44-45 0.5608589289491803 2306 46-47 0.518734204540273 2306 48-49 0.4254763881859205 2306 50-51 0.5953241500556317 2306 52-53 0.36692890724589233 2306 54-55 0.5395263565500841 2306 56-57 0.4451263479812937 2306 58-59 0.509858305486361 2306 60-61 0.5736580179683841 2306 62-63 0.4801292728648576 2306 64-65 0.563125653232369 2306 66-67 0.558854291699241 2306 68-69 0.5158256216089043 2306 70-71 0.5453183274681024 2306 72-73 0.41840172622023175 2306 74-75 0.45052173070440205 2306 76-77 0.49701477733999155 2306 78-79 0.42329578614845786 2306 80-81 0.33463407989567884 2306 82-83 0.4755940675648631 2306 84-85 0.5410823039064994 2306 86-87 0.43579785839634866 2306 88-89 0.6088434847158872 2306 90-91 0.4821208129724006 2306 92-93 0.4317420180088334 2306 94-95 0.2327711997278321 2306 96-97 0.31557360675269663 2306 98-99 0.10645649810986058 2306 100-101 0.1624760222706314 2307 1 0.1298576755136871 2307 2 0.11676906354164629 2307 3 -0.018413711763383134 2307 4 0.17528228986284233 2307 5 0.14891866153557487 2307 6 0.1392244742316393 2307 7 0.26277550507080605 2307 8 0.00990308029619058 2307 9 0.20873767552456002 2307 10-11 0.07963302454722054 2307 12-13 -0.017551444132351435 2307 14-15 0.2757449502044196 2307 16-17 0.20263992704224165 2307 18-19 0.33095332478148265 2307 20-21 0.1421393534116575 2307 22-23 0.16339743432374831 2307 24-25 0.28747187528983176 2307 26-27 0.11774952012584095 2307 28-29 0.16720059979172675 2307 30-31 0.12742065785874246 2307 32-33 0.14899790275504188 2307 34-35 0.03164469003830561 2307 36-37 0.12039577164770066 2307 38-39 0.013102623464988028 2307 40-41 0.13449932028074585 2307 42-43 0.04073268379523398 2307 44-45 -0.06496864094635413 2307 46-47 0.014372284349327913 2307 48-49 -0.04697520357409957 2307 50-51 0.022837003784374588 2307 52-53 0.05196772349708567 2307 54-55 -0.07554319116562169 2307 56-57 -0.0651453503185877 2307 58-59 -0.021185884133572586 2307 60-61 0.1263159495615085 2307 62-63 -0.008023654817264969 2307 64-65 -0.04059133904148382 2307 66-67 0.07251912705093844 2307 68-69 0.12482756010269469 2307 70-71 0.07040265349369434 2307 72-73 -0.3034292074412335 2307 74-75 -0.06203952353508413 2307 76-77 0.05534257138352672 2307 78-79 -0.02372474580886319 2307 80-81 -0.13275856866808056 2307 82-83 -0.10169587483682818 2307 84-85 -0.03279456000009873 2307 86-87 0.016380148539703043 2307 88-89 0.07669014892980996 2307 90-91 0.13565910518101632 2307 92-93 0.08543226710683172 2307 94-95 -0.11245923988051132 2307 96-97 -0.04738292090098284 2307 98-99 0.06376124973840902 2307 100-101 -0.22785191399094984 2201 1 0.23498903371621438 2201 2 0.22388287218291936 2201 3 0.25437802700170664 2201 4 0.2378660631063525 2201 5 0.3125292334334375 2201 6 0.2830486038536222 2201 7 0.3221922178511818 2201 8 0.16527027719735088 2201 9 0.4131408762553548 2201 10-11 0.3589307493113125 2201 12-13 0.30290562326978687 2201 14-15 0.5506611403627275 2201 16-17 0.4618080649559104 2201 18-19 0.40078807666043303 2201 20-21 0.35998316407314235 2201 22-23 0.26852967088269253 2201 24-25 0.3676890928323644 2201 26-27 0.48131251438431377 2201 28-29 0.3259235573934518 2201 30-31 0.3519127482591884 2201 32-33 0.3353508790657642 2201 34-35 0.3058246711243626 2201 36-37 0.2870133967476107 2201 38-39 0.3182421796607713 2201 40-41 0.2479968769859724 2201 42-43 0.2582765357394763 2201 44-45 0.5068327509557875 2201 46-47 0.2949027823481387 2201 48-49 0.2900707959515927 2201 50-51 0.23189534714560978 2201 52-53 0.2964472534007285 2201 54-55 0.44653107679749837 2201 56-57 0.4658821510209066 2201 58-59 0.4570254601323711 2201 60-61 0.4353567257943496 2201 62-63 0.5764247519905865 2201 64-65 0.5095260679332512 2201 66-67 0.515902175357617 2201 68-69 0.5916729723007208 2201 70-71 0.4564785083540741 2201 72-73 0.3672531583825034 2201 74-75 0.5151150115654985 2201 76-77 0.36954837614832314 2201 78-79 0.32973305062911606 2201 80-81 0.522542897894354 2201 82-83 0.535938769882641 2201 84-85 0.4316444483940529 2201 86-87 0.45675116629129064 2201 88-89 0.749709967578486 2201 90-91 0.6653753667789779 2201 92-93 0.5703548399884539 2201 94-95 0.3865416296923456 2201 96-97 0.5483877929392804 2201 98-99 0.42487450794299164 2201 100-101 0.3871861334812863 1101 1 0.09466527262550883 1101 2 0.04324288161380707 1101 3 0.05846550940747619 1101 4 0.27570504705910537 1101 5 0.14971296711562587 1101 6 0.18783074105230924 1101 7 0.3794764057769129 1101 8 0.2505338590684332 1101 9 0.22282521983441228 1101 10-11 0.18548249339804102 1101 12-13 0.2166275932557724 1101 14-15 0.3509032707829576 1101 16-17 0.4058253996711372 1101 18-19 0.329506960322675 1101 20-21 0.33695730608333463 1101 22-23 0.2588523677238399 1101 24-25 0.39416884998699686 1101 26-27 0.26189549938057866 1101 28-29 0.2493782260925741 1101 30-31 0.34202996306895983 1101 32-33 0.2833422308100708 1101 34-35 0.3033845378912332 1101 36-37 0.47473514951752094 1101 38-39 0.26777969500369636 1101 40-41 0.30648980682136084 1101 42-43 0.32777571626731117 1101 44-45 0.40456723651664817 1101 46-47 0.3143452574760843 1101 48-49 0.3685808956799619 1101 50-51 0.2691528142207318 1101 52-53 0.4396383484152935 1101 54-55 0.3694582597507363 1101 56-57 0.4431735212667789 1101 58-59 0.41530210171004 1101 60-61 0.6099425007617825 1101 62-63 0.5928480329682912 1101 64-65 0.4239414374434247 1101 66-67 0.48302859783631646 1101 68-69 0.4271114731387513 1101 70-71 0.4097657296639525 1101 72-73 0.42155471868152006 1101 74-75 0.3936090929734988 1101 76-77 0.4023983491600447 1101 78-79 0.37687098337617897 1101 80-81 0.4544230120910697 1101 82-83 0.636136911320861 1101 84-85 0.6410878076677875 1101 86-87 0.46946690195789387 1101 88-89 0.688677847435077 1101 90-91 0.6697840888226416 1101 92-93 0.6506512416788155 1101 94-95 0.5617261132611446 1101 96-97 0.5886794628123404 1101 98-99 0.46173451873830373 1101 100-101 0.5103356354397768 2304 1 0.3421895738298524 2304 2 0.3295331154000749 2304 3 0.31130762750050067 2304 4 0.4577560323454861 2304 5 0.2925983129555263 2304 6 0.34595513515456844 2304 7 0.4595050945386774 2304 8 0.2796064520828594 2304 9 0.41241276639219393 2304 10-11 0.38404961726517683 2304 12-13 0.4613709742790988 2304 14-15 0.5659290481628787 2304 16-17 0.4085277099819322 2304 18-19 0.4192496013377749 2304 20-21 0.3704862081415783 2304 22-23 0.34805254942273933 2304 24-25 0.5754648476435449 2304 26-27 0.6047122472262387 2304 28-29 0.35613260635231825 2304 30-31 0.2993350733250608 2304 32-33 0.30549600298279245 2304 34-35 0.33377455039199333 2304 36-37 0.3833191681053023 2304 38-39 0.22894449867619215 2304 40-41 0.2759980111824447 2304 42-43 0.29404760092230475 2304 44-45 0.27554705861764006 2304 46-47 0.1807886243137844 2304 48-49 0.3902187105611503 2304 50-51 0.297198869874471 2304 52-53 0.3482628242001198 2304 54-55 0.41118230479445117 2304 56-57 0.32624633244279977 2304 58-59 0.4371591024182919 2304 60-61 0.4918067171903786 2304 62-63 0.47527071235114704 2304 64-65 0.5265871076783526 2304 66-67 0.5943821676206227 2304 68-69 0.4838005841609814 2304 70-71 0.41054197012514493 2304 72-73 0.25310144853652616 2304 74-75 0.19928754224401501 2304 76-77 0.3008311961022727 2304 78-79 0.3156486178182618 2304 80-81 0.29942175996642106 2304 82-83 0.3761857211367001 2304 84-85 0.38148972175411444 2304 86-87 0.436020194584799 2304 88-89 0.3390502072828987 2304 90-91 0.6179512497669997 2304 92-93 0.5680720851243137 2304 94-95 0.4730641659179824 2304 96-97 0.5803760072043822 2304 98-99 0.46926839139850784 2304 100-101 0.3469925189757994 2202 1 0.20553712191136597 2202 2 0.11837629608810474 2202 3 0.11360470975780146 2202 4 0.23761065108622859 2202 5 0.2628171837213884 2202 6 0.23143014047045085 2202 7 0.28164135377090815 2202 8 0.16372862859687842 2202 9 0.21650038549721984 2202 10-11 0.2662322448480978 2202 12-13 0.17988344142407442 2202 14-15 0.30662757279974784 2202 16-17 0.31320147517285335 2202 18-19 0.23620141207376832 2202 20-21 0.18731699140697344 2202 22-23 0.09912726618617285 2202 24-25 0.16553200832234438 2202 26-27 0.20994336066222274 2202 28-29 0.2462174188637931 2202 30-31 0.26674125132299054 2202 32-33 0.1752113659850778 2202 34-35 0.2255820908817796 2202 36-37 0.2618105774271413 2202 38-39 0.2562749412229408 2202 40-41 0.18937523012903057 2202 42-43 0.22646703334173424 2202 44-45 0.34422285305176814 2202 46-47 0.24080246354193946 2202 48-49 0.17195535430673914 2202 50-51 0.2832090749299283 2202 52-53 0.3400810323286265 2202 54-55 0.4024086503221369 2202 56-57 0.3034550180055433 2202 58-59 0.42272454347850896 2202 60-61 0.43551196124369795 2202 62-63 0.38570500244730255 2202 64-65 0.3713527532893508 2202 66-67 0.47604145902631245 2202 68-69 0.5504201075184483 2202 70-71 0.4793390312146002 2202 72-73 0.3692960721901244 2202 74-75 0.5115277726841398 2202 76-77 0.4367622668916198 2202 78-79 0.45911376824512473 2202 80-81 0.4234965635539112 2202 82-83 0.48048186148455585 2202 84-85 0.490872125268794 2202 86-87 0.4362757634629375 2202 88-89 0.5587424530815603 2202 90-91 0.48366959683790967 2202 92-93 0.4850571664554906 2202 94-95 0.5056722194111671 2202 96-97 0.5713867277029188 2202 98-99 0.4576330112897331 2202 100-101 0.5345691926290499 1102 1 0.16885017578502826 1102 2 0.15740578600403765 1102 3 0.1389672866297218 1102 4 0.0639466604600969 1102 5 0.23091719494549778 1102 6 0.28476818470451803 1102 7 0.24138182093830096 1102 8 0.1718162126307945 1102 9 0.17836628938515986 1102 10-11 0.1629356053751323 1102 12-13 0.2849556571814915 1102 14-15 0.24884943548159555 1102 16-17 0.2531687201233268 1102 18-19 0.28922658704431825 1102 20-21 0.30247788687452726 1102 22-23 0.31399585072413316 1102 24-25 0.2756887241331043 1102 26-27 0.3160551320830507 1102 28-29 0.3593102657850977 1102 30-31 0.29000593318853163 1102 32-33 0.35351388832209807 1102 34-35 0.405007946397717 1102 36-37 0.37113700850729003 1102 38-39 0.3486936407331598 1102 40-41 0.4019686846966124 1102 42-43 0.1912684921537391 1102 44-45 0.3994587403432419 1102 46-47 0.3929030205298929 1102 48-49 0.39024349041950046 1102 50-51 0.22835220311090154 1102 52-53 0.3470455136582231 1102 54-55 0.45498094271235345 1102 56-57 0.43793832738104044 1102 58-59 0.5934295860091368 1102 60-61 0.47043635290024355 1102 62-63 0.5441583611881526 1102 64-65 0.6089199740860849 1102 66-67 0.44881503409983026 1102 68-69 0.5936271799540762 1102 70-71 0.5575740936374913 1102 72-73 0.6009354863882308 1102 74-75 0.7177294752921668 1102 76-77 0.6893543570495879 1102 78-79 0.508270081416768 1102 80-81 0.5817600112279848 1102 82-83 0.6167590698748384 1102 84-85 0.6697226009568311 1102 86-87 0.559715767353353 1102 88-89 0.7039298093374349 1102 90-91 0.559219054035669 1102 92-93 0.4756745456689373 1102 94-95 0.5847091029521252 1102 96-97 0.705828090702024 1102 98-99 0.7672444011598678 1102 100-101 0.4813950158324509 2305 1 0.27192312777467365 2305 2 0.12642097951105669 2305 3 0.13956947299904243 2305 4 0.13281076911991363 2305 5 0.16591907444363585 2305 6 0.23580215034452578 2305 7 0.270625317995588 2305 8 0.3734739497589814 2305 9 0.183501899954031 2305 10-11 0.16151142692233122 2305 12-13 0.1856802580874728 2305 14-15 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11 15893.0 12 10575.0 13 7585.0 14 5984.0 15 5608.0 16 5499.0 17 5634.0 18 6086.0 19 6383.0 20 7159.0 21 8071.0 22 8821.0 23 9485.0 24 10668.0 25 12266.0 26 14651.0 27 17734.0 28 21856.0 29 26907.0 30 34081.0 31 45293.0 32 63550.0 33 91433.0 34 142092.0 35 252300.0 36 489108.0 37 703465.0 38 156146.0 39 138.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.97433541779599 20.8415776992536 27.1075648194355 12.07652206351491 2 36.4645171355485 19.342776422403396 33.22874385934711 10.963962582700999 3 43.37304475332432 15.244918957870999 27.279253860301367 14.10278242850331 4 35.90186004488775 13.677406979829195 34.944768713385294 15.475964261897762 5 34.90695640754066 12.779716824043916 36.77235527112558 15.540971497289844 6 27.292347702374343 13.396328155781076 43.99960196810635 15.311722173738232 7 37.60075499870717 13.982353787339342 31.40284657098032 17.014044642973175 8 33.76632571578624 26.645970025120967 23.21511631554372 16.372587943549075 9 34.85423043562412 27.735643472089805 20.31401208593487 17.09611400635121 10-11 31.13293570043545 26.525857822907245 26.182914567461673 16.158291909195633 12-13 31.582494434037322 22.755147605274946 26.28371140654347 19.378646554144265 14-15 29.160165754223836 23.43205161179314 26.399313001795015 21.008469632188007 16-17 27.710551037220572 26.13259028560841 27.753970666881152 18.40288801028987 18-19 26.956588557179266 26.384091319974097 27.154380695989072 19.50493942685757 20-21 25.764583457016087 27.927360791091704 27.31614208376226 18.99191366812995 22-23 27.4833857169796 25.25415810589335 27.31313326766901 19.949322909458036 24-25 27.385346513656067 25.362962849803722 27.71516160962168 19.536529026918537 26-27 23.808564502976388 26.06063597216879 29.497317493665292 20.633482031189534 28-29 23.363861490483234 27.176756863902302 27.249048592419058 22.21033305319541 30-31 22.862883912666764 27.328769703525513 27.941715953509817 21.86663043029791 32-33 22.343143560463897 24.892526898280185 30.213497001880146 22.55083253937577 34-35 22.298528984879777 26.777639242144787 28.403957343388058 22.519874429587382 36-37 21.112780207650363 29.624619266169255 28.416925610906908 20.845674915273474 38-39 20.46612070899708 27.972761947498313 27.918420462194298 23.6426968813103 40-41 22.720980978398057 27.63978437322952 26.214000033656625 23.425234614715798 42-43 25.11578828285875 27.054818350327288 26.70923796787243 21.12015539894153 44-45 21.699427565917656 29.25241154489886 26.40229072363288 22.6458701655506 46-47 22.30603661109465 29.328512303949296 27.115843859696376 21.24960722525968 48-49 22.121884208476082 27.018493469751544 28.699658834796516 22.159963486975855 50-51 20.690044454199967 27.15276987573158 27.227619553563752 24.929566116504702 52-53 22.705306746043146 28.335320139028525 26.48765816648953 22.4717149484388 54-55 23.213114239175795 27.99573268957224 27.48316184818067 21.307991223071294 56-57 24.72157906974865 27.47102222788815 25.14880538231088 22.658593320052322 58-59 22.80829865983497 26.15358341937657 26.03807864871761 25.000039272070847 60-61 20.551715907092273 26.0611353594234 28.502076461150256 24.88507227233407 62-63 22.21963546975911 26.088841490014275 28.539490402288603 23.152032637938014 64-65 22.813678405223808 28.295586983183934 27.364122559975023 21.526612051617235 66-67 22.041519867812614 27.85661634567199 26.84816261596792 23.25370117054748 68-69 21.25212195257101 24.69260711387754 30.79889509281853 23.25637584073292 70-71 21.069770174956982 29.086732982019853 28.257140652782788 21.586356190240373 72-73 23.49697421013202 29.868695523445947 26.481089374071964 20.15324089235007 74-75 24.760971277077733 28.064533493627845 26.942643872547922 20.2318513567465 76-77 23.351012670266236 27.247263089707495 26.663656558805215 22.738067681221054 78-79 23.712998453184607 28.740058750273654 26.384664455904993 21.162278340636743 80-81 22.704895230403373 29.626688203885678 26.296248934356353 21.372167631354603 82-83 22.4561031109332 29.255714566835934 26.989543909454593 21.29863841277627 84-85 22.924311252456327 28.41948296310989 26.882750134736177 21.773455649697613 86-87 20.563266826426847 29.112164211924572 27.993102488848198 22.33146647280038 88-89 20.07236437793268 29.4096846477442 28.426801744445505 22.09114922987761 90-91 21.596910429923447 28.481104938072217 27.089255143899997 22.832729488104338 92-93 21.986192413160964 28.68177085003699 26.035462483288864 23.29657425351318 94-95 22.65063354055099 29.950435533895742 25.77345508604363 21.62547583950964 96-97 21.298840694054064 31.25362612500984 24.67471682304465 22.772816357891447 98-99 21.604116761816275 29.08267192933619 26.508260194384697 22.80495111446284 100-101 23.1603887355102 29.754869570777753 26.2430124197473 20.84172927396475 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1258.0 1 785.0 2 265.0 3 238.5 4 227.0 5 197.0 6 226.5 7 299.0 8 397.0 9 514.0 10 612.0 11 749.0 12 847.0 13 840.0 14 804.5 15 730.5 16 652.0 17 590.0 18 558.0 19 562.0 20 589.0 21 655.5 22 736.5 23 863.0 24 1008.0 25 1185.5 26 1512.0 27 1944.5 28 2387.0 29 2907.0 30 3769.0 31 4930.0 32 6600.0 33 9400.5 34 14206.0 35 23523.0 36 35502.5 37 48470.0 38 64742.5 39 79260.5 40 89279.0 41 96404.0 42 132219.5 43 170060.5 44 160089.5 45 138009.5 46 134691.0 47 130864.0 48 115766.0 49 104957.5 50 95275.5 51 89659.5 52 88157.0 53 89312.0 54 76710.0 55 48429.0 56 31960.5 57 25947.0 58 22218.0 59 18219.0 60 14178.5 61 11234.0 62 8622.0 63 6658.0 64 5237.5 65 4019.0 66 3095.5 67 2429.0 68 1878.5 69 1386.0 70 1034.5 71 811.5 72 594.5 73 407.5 74 300.0 75 216.5 76 162.5 77 137.0 78 102.5 79 72.5 80 65.0 81 50.0 82 34.5 83 25.0 84 21.5 85 24.0 86 27.5 87 25.0 88 18.0 89 13.0 90 9.5 91 7.0 92 6.0 93 5.0 94 3.0 95 2.5 96 1.5 97 2.0 98 3.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25737165182065286 2 0.2432844111041445 3 0.20723896342952308 4 0.21117444655032544 5 0.22427781648663322 6 0.22758720002003519 7 0.20299043051502058 8 0.1996363255825186 9 0.1811216663551076 10-11 0.1687114781048502 12-13 0.19773566612076746 14-15 0.19355421530491496 16-17 0.19482877517926572 18-19 0.18682364474036098 20-21 0.18843361510796192 22-23 0.15648017545099296 24-25 0.4662205856177769 26-27 0.3252810963540432 28-29 0.277809331209365 30-31 0.2800454011643663 32-33 0.32434194697294266 34-35 0.3218599093228912 36-37 0.3122671692159355 38-39 0.338362105590801 40-41 0.34339326298955397 42-43 0.38053438494212605 44-45 0.34768651730315653 46-47 0.37235036890682116 48-49 0.34972134096220775 50-51 0.36973416705946965 52-53 0.35929172036961343 54-55 0.3352316076537991 56-57 0.3722609261086211 58-59 0.358643260082663 60-61 0.32530345705359326 62-63 0.30224957581752954 64-65 0.308040997000983 66-67 0.31792442620208883 68-69 0.32563886754684346 70-71 0.30043835915397843 72-73 0.3083987681937832 74-75 0.3057154842477816 76-77 0.30902486778118354 78-79 0.31170815172718513 80-81 0.32941782577079565 82-83 0.3464790395274558 84-85 0.34153732492690286 86-87 0.34551752944680525 88-89 0.3408441432408525 90-91 0.31385477888398644 92-93 0.2969277293246264 94-95 0.3062521410369819 96-97 0.29451277377322493 98-99 0.3145032391709368 100-101 0.3380266950975508 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2236066.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.61482888421457 #Duplication Level Percentage of deduplicated Percentage of total 1 87.81065124344227 40.05467830681062 2 5.196798409192078 4.741021403621102 3 1.9597301636821618 2.6817826822678685 4 1.078615271137261 1.9680340409930743 5 0.7020203229612121 1.6011268452558365 6 0.46590910842168964 1.2751418553751406 7 0.37018936742504993 1.18202872548945 8 0.2836663885140247 1.0351515017816293 9 0.18495600456035052 0.7593062849215558 >10 1.5862626573055947 14.27735294004992 >50 0.19705983909556374 6.217429884383185 >100 0.14960625930474086 13.127834417627598 >500 0.009086114499464398 2.777995116198994 >1k 0.004658994744057788 3.503521646528725 >5k 2.9619589296020607E-4 0.7698975414099726 >10k+ 4.93659821578991E-4 4.02769680728521 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 32440 1.4507621868048617 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 22242 0.9946933587827907 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 12291 0.5496707163384266 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 11994 0.5363884608057187 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10465 0.468009441581778 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 5886 0.2632301551027563 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5871 0.2625593341162559 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5338 0.2387228283959418 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 3857 0.17249043632880245 Illumina Single End PCR Primer 1 (96% over 32bp) GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 3061 0.13689220264518132 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 2956 0.13219645573967853 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 2940 0.1314809133540781 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 2670 0.11940613559707092 No Hit TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA 2600 0.11627563766006907 No Hit GTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC 2412 0.10786801462926408 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2371 0.10603443726616298 No Hit AACTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAA 2311 0.1033511533201614 No Hit GTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCT 2286 0.10223311834266073 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0027280053451016204 0.0 0.0 2 0.0028174481433016737 0.0 0.0 3 0.0031304979370018597 0.0 0.0 4 0.0032199407352019125 0.0 0.0 5 0.0036224333271021518 0.0 0.0 6 0.003980204519902364 0.0 0.0 7 0.00429325431360255 0.0 0.0 8 0.004785189703702842 0.0 0.0 9 0.005456010690203241 0.0 0.0 10-11 0.00885483702180526 0.0 0.0 12-13 0.011918252860157078 0.0 0.0 14-15 0.015004029398058913 0.0 0.0 16-17 0.018291052231910868 0.0 0.0 18-19 0.021913485559013016 0.0 0.0 20-21 0.025401754688815087 0.0 0.0 22-23 0.028711138222217054 0.0 0.0 24-25 0.03228885015021918 0.0 0.0 26-27 0.03673862936067182 0.0 0.0 28-29 0.04239588634682519 0.0 0.0 30-31 0.09389257741050577 0.0 0.0 32-33 0.10319462842331129 0.0 0.0 34-35 0.11135628375906614 0.0 0.0 36-37 0.11837754341777032 0.0 0.0 38-39 0.12638267385667507 0.0 0.0 40-41 0.13546111787398046 0.0 0.0 42-43 0.14693215674313728 0.0 0.0 44-45 0.16081815116369552 0.0 0.0 46-47 0.1753749665707542 0.0 0.0 48-49 0.1917653593409139 0.0 0.0 50-51 0.21032473996742493 0.0 0.0 52-53 0.230628255158837 0.0 0.0 54-55 0.25316784030525036 0.0 0.0 56-57 0.2823485532180177 0.0 0.0 58-59 0.3151069780587872 0.0 0.0 60-61 0.3507946545406084 0.0 0.0 62-63 0.3934365085824837 0.0 0.0 64-65 0.43589947702795895 0.0 0.0 66-67 0.48256625698883665 0.0 0.0 68-69 0.5350691795322677 0.0 0.0 70-71 0.5939225407479027 0.0 0.0 72-73 0.6600878507163921 0.0 0.0 74-75 0.7292494944245831 0.0 0.0 76-77 0.8099045377014811 0.0 0.0 78-79 0.9022095054439359 0.0 0.0 80-81 1.0039506883964964 0.0 0.0 82-83 1.1193766194736647 0.0 0.0 84-85 1.252646388791744 0.0 0.0 86-87 1.3941672562437781 0.0 0.0 88-89 1.5483442796411198 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 930 0.0 70.8827 9 ATTCGGA 270 0.0 65.01473 2 CGGGAGA 1285 0.0 63.868633 4 AGAGCGG 1060 0.0 62.189537 8 AAGCGGC 385 0.0 60.359234 9 TCGGGAG 1425 0.0 59.592625 3 ATCCGGA 280 0.0 59.303978 2 ATCGGGA 1770 0.0 56.020485 2 GGTTCGG 310 0.0 55.10149 1 GTTCGGA 240 0.0 53.37358 2 CGGACCG 45 1.5272144E-5 52.71465 2 TTGTGTT 29390 0.0 51.40389 1 GGATCGG 705 0.0 51.150158 1 GATCCGG 335 0.0 50.989433 1 TCGGGAA 415 0.0 48.012638 3 TCGGAGA 390 0.0 47.439995 4 TCGGAAA 540 0.0 43.925922 4 AAGGGGC 1030 0.0 43.28117 9 GATCGGG 2610 0.0 41.99465 1 TGTGTTT 53435 0.0 41.778835 1 >>END_MODULE