##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_53115004-1.3410000003588d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4698037 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.635071839578956 33.0 33.0 33.0 33.0 33.0 2 32.63541049165854 33.0 33.0 33.0 33.0 33.0 3 32.70434992317004 33.0 33.0 33.0 33.0 33.0 4 36.63224619133481 37.0 37.0 37.0 37.0 37.0 5 36.60420533086479 37.0 37.0 37.0 37.0 37.0 6 36.503456017907055 37.0 37.0 37.0 37.0 37.0 7 36.37009627638096 37.0 37.0 37.0 37.0 37.0 8 36.32460600033588 37.0 37.0 37.0 37.0 37.0 9 36.37391489253916 37.0 37.0 37.0 37.0 37.0 10-11 36.30870212388706 37.0 37.0 37.0 35.0 37.0 12-13 36.32455619229904 37.0 37.0 37.0 35.0 37.0 14-15 38.16626593192008 40.0 37.0 40.0 37.0 40.0 16-17 38.205835650081085 40.0 37.0 40.0 37.0 40.0 18-19 38.155332216412944 40.0 37.0 40.0 37.0 40.0 20-21 38.17534727802271 40.0 37.0 40.0 37.0 40.0 22-23 37.89580435403127 40.0 37.0 40.0 33.0 40.0 24-25 38.050621568114515 40.0 37.0 40.0 37.0 40.0 26-27 38.0340979647457 40.0 37.0 40.0 37.0 40.0 28-29 37.81754209258037 40.0 37.0 40.0 33.0 40.0 30-31 37.77320399988336 40.0 37.0 40.0 33.0 40.0 32-33 37.588034534423635 40.0 37.0 40.0 33.0 40.0 34-35 37.54618599214949 40.0 37.0 40.0 33.0 40.0 36-37 37.60304144049951 40.0 37.0 40.0 33.0 40.0 38-39 37.40663824060985 40.0 37.0 40.0 33.0 40.0 40-41 37.08642822523535 40.0 37.0 40.0 33.0 40.0 42-43 36.90450394494552 37.0 37.0 40.0 33.0 40.0 44-45 36.83685558457713 37.0 37.0 40.0 33.0 40.0 46-47 37.069128765907976 37.0 37.0 40.0 33.0 40.0 48-49 36.74372647554713 37.0 37.0 40.0 33.0 40.0 50-51 36.736723657135954 37.0 37.0 40.0 33.0 40.0 52-53 36.67302960789793 37.0 37.0 40.0 33.0 40.0 54-55 36.28285813840972 37.0 37.0 40.0 33.0 40.0 56-57 36.23817639154396 37.0 37.0 40.0 33.0 40.0 58-59 36.139209312314904 37.0 37.0 40.0 33.0 40.0 60-61 35.84834644767591 37.0 37.0 40.0 30.0 40.0 62-63 35.598686430098354 37.0 35.0 40.0 27.0 40.0 64-65 35.270884946202 37.0 33.0 38.5 27.0 40.0 66-67 34.43407906323428 37.0 33.0 37.0 27.0 40.0 68-69 34.25658588895745 37.0 33.0 37.0 27.0 40.0 70-71 33.64606015235725 37.0 33.0 37.0 27.0 38.5 72-73 32.52542849279391 37.0 33.0 37.0 22.0 37.0 74-75 31.492869255818974 35.0 30.0 37.0 15.0 37.0 76-77 29.05329949508699 33.0 27.0 35.0 15.0 37.0 78-79 30.837855683128932 33.0 30.0 37.0 15.0 37.0 80-81 31.046738350506818 35.0 33.0 37.0 15.0 37.0 82-83 30.966871163424216 37.0 33.0 37.0 15.0 37.0 84-85 30.79713324948271 33.0 33.0 37.0 15.0 37.0 86-87 30.65605815790723 33.0 33.0 37.0 15.0 37.0 88-89 30.462278926283467 33.0 33.0 37.0 15.0 37.0 90-91 30.2251938415981 33.0 27.0 37.0 6.0 37.0 92-93 30.20087464189831 33.0 27.0 37.0 6.0 37.0 94-95 30.127910657153187 33.0 27.0 37.0 6.0 37.0 96-97 29.968885089666173 33.0 27.0 37.0 6.0 37.0 98-99 29.815889381032974 33.0 27.0 37.0 6.0 37.0 100-101 28.431144752584963 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.17004452217978638 2306 2 0.16941699818941913 2306 3 0.09528807650541182 2306 4 0.13499675183172144 2306 5 0.15154183830227197 2306 6 0.14705767413530424 2306 7 0.15325965158534416 2306 8 0.17269541735939242 2306 9 0.2128996882981653 2306 10-11 0.16335958835556852 2306 12-13 0.19578478647272135 2306 14-15 0.36881251382127545 2306 16-17 0.4008571212668386 2306 18-19 0.39631505614573825 2306 20-21 0.3764468416869917 2306 22-23 0.46190204312044614 2306 24-25 0.42746203019980555 2306 26-27 0.3753519490472854 2306 28-29 0.3836693496544612 2306 30-31 0.3525596497874872 2306 32-33 0.3780588442622417 2306 34-35 0.3974684146150125 2306 36-37 0.4284919593064629 2306 38-39 0.4283799494398224 2306 40-41 0.4245566977581845 2306 42-43 0.4770201286143845 2306 44-45 0.4435172243573433 2306 46-47 0.4839337951101186 2306 48-49 0.5075129880247076 2306 50-51 0.42910226628612236 2306 52-53 0.2690758590536859 2306 54-55 0.42890409987529665 2306 56-57 0.3981501717536915 2306 58-59 0.4147408352185167 2306 60-61 0.32693761906743646 2306 62-63 0.3112793837671859 2306 64-65 0.296522839916598 2306 66-67 0.34658324997808876 2306 68-69 0.42062659666844837 2306 70-71 0.3512783557294057 2306 72-73 0.1637909567700575 2306 74-75 0.09807051092903052 2306 76-77 -0.13932366147494335 2306 78-79 0.4730818655862059 2306 80-81 0.5733052363274034 2306 82-83 0.40194569840545213 2306 84-85 0.5319393796814289 2306 86-87 0.4507830800967909 2306 88-89 0.5332709245837499 2306 90-91 0.5277028970309772 2306 92-93 0.49411590428484686 2306 94-95 0.336239093195811 2306 96-97 0.37101522878899473 2306 98-99 0.21688074829366855 2306 100-101 0.22382329974451665 2307 1 0.12940131017570167 2307 2 0.1764547987867786 2307 3 0.11693781539269565 2307 4 0.13477227340295883 2307 5 0.18531107597278407 2307 6 0.1403472457011219 2307 7 0.1599427912180289 2307 8 0.11659693095332813 2307 9 0.1377057695888837 2307 10-11 0.22552871163740207 2307 12-13 0.21843280952989375 2307 14-15 0.3310656529680003 2307 16-17 0.3873716404045169 2307 18-19 0.4114009971794985 2307 20-21 0.3525978309994997 2307 22-23 0.46596606526549067 2307 24-25 0.4250204301041123 2307 26-27 0.38795166531851066 2307 28-29 0.440016986556401 2307 30-31 0.3497514732401612 2307 32-33 0.3956735319923723 2307 34-35 0.464980302065527 2307 36-37 0.39287783043602786 2307 38-39 0.33418774469156887 2307 40-41 0.4335167701295859 2307 42-43 0.40983580023664956 2307 44-45 0.5130195588582396 2307 46-47 0.3989352530049928 2307 48-49 0.5200229409608639 2307 50-51 0.4565562958701719 2307 52-53 0.32249354780434203 2307 54-55 0.5200464539643121 2307 56-57 0.5360551114005787 2307 58-59 0.49406970826577634 2307 60-61 0.3719482542236392 2307 62-63 0.3054281320336045 2307 64-65 0.23616066518602707 2307 66-67 0.1716104920521886 2307 68-69 0.4409374086411262 2307 70-71 0.4256087520899072 2307 72-73 0.1317292317439822 2307 74-75 0.15191276483450267 2307 76-77 -0.00788855088053353 2307 78-79 0.6206891423507237 2307 80-81 0.7879257257314976 2307 82-83 0.49161758794935295 2307 84-85 0.5499434935617096 2307 86-87 0.47203811263774753 2307 88-89 0.6818641494845927 2307 90-91 0.755708403387775 2307 92-93 0.4990815316095336 2307 94-95 0.6336746934933686 2307 96-97 0.48309000945058145 2307 98-99 0.45050251429032784 2307 100-101 0.54490319799093 2201 1 0.0864923565770539 2201 2 0.16097919374269765 2201 3 0.08775168310845771 2201 4 0.12631570661100966 2201 5 0.170004075375644 2201 6 0.05425711257385757 2201 7 0.16147076901845026 2201 8 0.094999755077815 2201 9 0.10315766463261866 2201 10-11 0.17288021678334786 2201 12-13 0.1082058960450425 2201 14-15 0.26426755679320735 2201 16-17 0.22726371599550532 2201 18-19 0.1591450345534895 2201 20-21 0.23426297554268416 2201 22-23 0.23018811380952542 2201 24-25 0.21198168620723834 2201 26-27 0.2719136063772112 2201 28-29 0.244263711063752 2201 30-31 0.19862261377898704 2201 32-33 0.2378707465064025 2201 34-35 0.1485260008958349 2201 36-37 0.23452137253445215 2201 38-39 0.19259908685321392 2201 40-41 0.2946499325167409 2201 42-43 0.2882513575094734 2201 44-45 0.18560728585953967 2201 46-47 0.21483939435172061 2201 48-49 0.48829456975007446 2201 50-51 0.3087903047921685 2201 52-53 0.25934898705405374 2201 54-55 0.3441551335807418 2201 56-57 0.2526745044245686 2201 58-59 0.332675296800673 2201 60-61 0.24198408266861549 2201 62-63 0.3090605919022309 2201 64-65 0.34112834933370095 2201 66-67 0.33584483467047477 2201 68-69 0.2839412022411807 2201 70-71 0.37929170547780444 2201 72-73 -0.16202010964489233 2201 74-75 0.17619575686091693 2201 76-77 0.16277866609231495 2201 78-79 0.4185016752812807 2201 80-81 0.4272224168692276 2201 82-83 0.36723712147705 2201 84-85 0.3968431914787409 2201 86-87 0.3837451448482234 2201 88-89 0.5465248330351251 2201 90-91 0.6092078238099887 2201 92-93 0.3818171387611713 2201 94-95 0.35832782496971305 2201 96-97 0.3630045656345011 2201 98-99 0.3045253110165902 2201 100-101 0.3621372947277308 1101 1 0.15572908383273187 1101 2 0.15432184099888957 1101 3 0.11874537842352595 1101 4 0.09394707577384054 1101 5 0.15161506063021335 1101 6 0.1426900612227726 1101 7 0.0984970080855021 1101 8 0.14543674946197171 1101 9 0.14122767752011356 1101 10-11 0.16770306629396003 1101 12-13 0.15268028194868322 1101 14-15 0.26739249110256225 1101 16-17 0.258546773549412 1101 18-19 0.29744684446214364 1101 20-21 0.2366770764338142 1101 22-23 0.2660304659631265 1101 24-25 0.32011762420497547 1101 26-27 0.31137723114254356 1101 28-29 0.3227325409536874 1101 30-31 0.3928554198084626 1101 32-33 0.231700919412269 1101 34-35 0.24905144520715083 1101 36-37 0.35798518163784365 1101 38-39 0.4742355398656102 1101 40-41 0.38478841243925643 1101 42-43 0.2394281296207481 1101 44-45 0.4532129447144797 1101 46-47 0.5316399486594889 1101 48-49 0.5769409624645121 1101 50-51 0.4419758442801225 1101 52-53 0.48638062690276485 1101 54-55 0.6035742423224093 1101 56-57 0.3970677977533086 1101 58-59 0.5554353872880213 1101 60-61 0.5704728016646428 1101 62-63 0.5170183364452541 1101 64-65 0.48322162115633915 1101 66-67 0.4251440104853188 1101 68-69 0.43300835465479537 1101 70-71 0.3812228891706724 1101 72-73 0.09042123770459654 1101 74-75 0.10827874456039766 1101 76-77 0.11233059525475753 1101 78-79 0.45393710925995734 1101 80-81 0.42920356587744024 1101 82-83 0.38858791518174485 1101 84-85 0.39005761059557287 1101 86-87 0.39516715125478186 1101 88-89 0.48057296143789685 1101 90-91 0.34877768550793675 1101 92-93 0.33716698490357544 1101 94-95 0.2489982172055214 1101 96-97 0.26375501556488956 1101 98-99 0.2797801907198405 1101 100-101 0.2606529691220416 2304 1 0.15428774374569088 2304 2 0.16150225559664477 2304 3 0.10107340990344227 2304 4 0.11684685926616112 2304 5 0.1533889165610418 2304 6 0.053597719951994804 2304 7 0.03319948307233744 2304 8 0.057447537169665 2304 9 0.10692641203227993 2304 10-11 0.14137122862478435 2304 12-13 0.14775103028018322 2304 14-15 0.18539053543625528 2304 16-17 0.2214605235833602 2304 18-19 0.2423940055478937 2304 20-21 0.23358608099791667 2304 22-23 0.17308133519195934 2304 24-25 0.2727332402705187 2304 26-27 0.1789228962136562 2304 28-29 0.24122497808015453 2304 30-31 0.15353382222536283 2304 32-33 0.232276579252499 2304 34-35 0.2567858338292339 2304 36-37 0.29001329171241963 2304 38-39 0.2891777679758576 2304 40-41 0.3168244082650702 2304 42-43 0.27207535878680744 2304 44-45 0.3121973125269406 2304 46-47 0.3117278134521584 2304 48-49 0.31744279414929366 2304 50-51 0.3051554317501939 2304 52-53 0.34816487450133593 2304 54-55 0.5578250565262266 2304 56-57 0.5028825316661525 2304 58-59 0.4039275440322214 2304 60-61 0.4186008995601114 2304 62-63 0.3377622869788155 2304 64-65 0.26907922727007616 2304 66-67 0.258574382368586 2304 68-69 0.30769369127560964 2304 70-71 0.361726683531316 2304 72-73 -0.02301200809689874 2304 74-75 0.16248276997354338 2304 76-77 0.31267664830483355 2304 78-79 0.4659655084787424 2304 80-81 0.5901386056873399 2304 82-83 0.44413865166709954 2304 84-85 0.5204421548736242 2304 86-87 0.42222995425382237 2304 88-89 0.4427999939683751 2304 90-91 0.6111692673808733 2304 92-93 0.37172344206335595 2304 94-95 0.3732500565570014 2304 96-97 0.3646547359711967 2304 98-99 0.2545035103757769 2304 100-101 0.4129557399121708 2202 1 0.07828309686444612 2202 2 0.12045292871106028 2202 3 0.0816240412120095 2202 4 0.05500434673444943 2202 5 0.13461286327466127 2202 6 0.06415852133801536 2202 7 0.047097516991357224 2202 8 0.11396502930092112 2202 9 0.07243859401636854 2202 10-11 0.12391768738773123 2202 12-13 0.07038106054923077 2202 14-15 0.17393713891176787 2202 16-17 0.16573432779529895 2202 18-19 0.068583160949089 2202 20-21 0.09942406724217534 2202 22-23 0.06763572098761728 2202 24-25 0.03391248688082982 2202 26-27 -0.017927193628722193 2202 28-29 0.03145909085144183 2202 30-31 0.05436189967873872 2202 32-33 0.16447813973632464 2202 34-35 0.19774149952713316 2202 36-37 0.19385118952123293 2202 38-39 0.179651661427215 2202 40-41 0.028332674970322103 2202 42-43 0.1817045730377771 2202 44-45 0.22435497686615236 2202 46-47 0.18154278235905252 2202 48-49 0.2277599960514607 2202 50-51 0.2240307739351124 2202 52-53 0.22485911695115846 2202 54-55 0.21676769005371455 2202 56-57 0.3414281301933002 2202 58-59 0.32156097102674863 2202 60-61 0.289900981161928 2202 62-63 0.22653661175400686 2202 64-65 0.2529982445109624 2202 66-67 0.08496352249456862 2202 68-69 0.1488875297361929 2202 70-71 0.328976421239787 2202 72-73 -0.19381263036093088 2202 74-75 -0.12294889554748067 2202 76-77 -0.24937127567283213 2202 78-79 0.20764996273148384 2202 80-81 0.2071495676641142 2202 82-83 0.2242193691198402 2202 84-85 0.28439465699406696 2202 86-87 0.2516347434357513 2202 88-89 0.26511547539893954 2202 90-91 0.38643206137851394 2202 92-93 0.34588400869344227 2202 94-95 0.2440766064888642 2202 96-97 0.2532964075621109 2202 98-99 0.06924389201496695 2202 100-101 0.1844449527964116 1102 1 0.13528581299129883 1102 2 0.15720474911726967 1102 3 0.06521480224732557 1102 4 0.02114043865564952 1102 5 0.15551952483097864 1102 6 0.06404034445596807 1102 7 0.03856592268062542 1102 8 0.007190118371966037 1102 9 0.04426496230362886 1102 10-11 0.05149326391493503 1102 12-13 0.13245695929162338 1102 14-15 0.25247106294163046 1102 16-17 0.1681718903830216 1102 18-19 0.09764202330714511 1102 20-21 0.1710678469233997 1102 22-23 0.11488367762979124 1102 24-25 0.1853484268066481 1102 26-27 0.1296878816581568 1102 28-29 0.21454260564610195 1102 30-31 0.21543091510310575 1102 32-33 0.257673574989866 1102 34-35 0.21408391563375773 1102 36-37 0.2677791586136138 1102 38-39 0.23584733654161738 1102 40-41 0.24051172014871014 1102 42-43 0.24165457175392646 1102 44-45 0.13087715710749848 1102 46-47 0.19955849418531812 1102 48-49 0.2173783730263139 1102 50-51 0.20306771689269 1102 52-53 0.2143216156751393 1102 54-55 0.23869194412085903 1102 56-57 0.278447335972011 1102 58-59 0.23580992781985088 1102 60-61 0.1802395043830245 1102 62-63 0.24675068288183866 1102 64-65 0.23879663362291126 1102 66-67 0.23731447213693713 1102 68-69 0.02172576017672867 1102 70-71 0.026752040478513095 1102 72-73 0.0785021961094472 1102 74-75 0.006510716078320655 1102 76-77 -0.2696886184903775 1102 78-79 0.09473711865169676 1102 80-81 0.11535334168386058 1102 82-83 0.0925251685548183 1102 84-85 0.08104324328667545 1102 86-87 0.06889836931792814 1102 88-89 0.2265458791104571 1102 90-91 0.27056025480031565 1102 92-93 0.18730422301507588 1102 94-95 0.07053451278530432 1102 96-97 0.03405089211488388 1102 98-99 -0.019762276104330567 1102 100-101 0.0682456077480964 2305 1 0.07731447446074924 2305 2 0.0914175567955624 2305 3 0.09378415423644526 2305 4 0.07563981333010616 2305 5 0.12262953585883452 2305 6 0.0772798598787574 2305 7 0.08032840343433634 2305 8 0.042254794967526266 2305 9 0.08770576958888654 2305 10-11 0.08311516209037251 2305 12-13 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-1.637721321583978 1213 98-99 -1.6210464774044482 1213 100-101 -1.655918997102816 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 11.0 8 123.0 9 314.0 10 239.0 11 127.0 12 123.0 13 165.0 14 370.0 15 853.0 16 1633.0 17 2799.0 18 4106.0 19 6027.0 20 8648.0 21 11996.0 22 16779.0 23 23459.0 24 31614.0 25 43256.0 26 61369.0 27 92388.0 28 158151.0 29 245592.0 30 193372.0 31 106802.0 32 121490.0 33 172012.0 34 265224.0 35 467268.0 36 939050.0 37 1404867.0 38 317748.0 39 62.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.10186373759093 23.146881388979633 19.945706733627368 13.805548139802069 2 26.738933729129847 32.014392394099914 28.430065578453302 12.816608298316936 3 32.88854472623353 17.343222286244234 34.18719350230746 15.58103948521478 4 26.05082176491863 17.265780447301864 28.846506314988847 27.836891472790658 5 40.411112130449375 16.161196687041844 27.594014265958318 15.833676916550466 6 35.30819361363054 17.18334700216282 31.61933377706476 15.889125607141876 7 28.733745604813244 29.51556149940922 24.54733753693298 17.203355358844554 8 25.274790300714955 35.5265614127773 22.38526431358459 16.81338397292316 9 37.765517810949554 21.901381364174018 22.736240689462427 17.596860135414 10-11 27.027509125291726 31.593433025424805 25.87369293552993 15.505364913753542 12-13 20.721931308757252 30.210905107814177 25.8102586250385 23.256904958390066 14-15 20.326191556175484 30.72932375798658 26.142216419325777 22.80226826651216 16-17 25.86354225723036 24.992887959040605 26.697202235367957 22.446367548361078 18-19 18.907641638411956 25.152728682213443 33.009989065645925 22.92964061372867 20-21 25.061009523764927 25.296203499461583 32.711949693031364 16.930837283742125 22-23 18.6770240421691 37.54229692103319 26.62145913282505 17.15921990397266 24-25 18.91135595568958 24.691099708239847 32.86923879058424 23.52830554548634 26-27 18.795679982937553 24.891726054945927 26.51967193957817 29.79292202253835 28-29 24.3817683853916 31.566971481918937 26.221994420222742 17.829265712466718 30-31 17.66746409191754 26.32725114766018 32.11179477726549 23.893489983156798 32-33 17.499415181276774 32.27098892580029 26.595767551426263 23.63382834149667 34-35 17.930765551654872 32.20229002027868 33.08157215449772 16.785372273568726 36-37 17.842100860423194 26.6714161680719 32.84744245309264 22.639040518412266 38-39 24.698100435937604 32.3296586574484 25.868405592262107 17.103835314351883 40-41 18.358042409382907 37.98123733697991 25.790966453187856 17.869753800449324 42-43 18.405343491626688 31.91865219836429 32.32899494284895 17.347009367160066 44-45 17.867043027724097 25.837207168796912 32.767411718803714 23.528338084675276 46-47 24.239308273967378 25.74793015642981 26.592098938671665 23.42066263093115 48-49 18.508857200728414 32.3049860264302 31.8401228196798 17.346033953161584 50-51 24.671328587968308 25.926039060602495 31.397552501959968 18.005079849469226 52-53 18.28800719499118 25.88350181072171 25.525870303897907 30.302620690389205 54-55 24.703237166924875 25.985176814379763 31.5682268701482 17.743359148547157 56-57 18.469144481527312 25.938505697693927 31.613121004354277 23.979228816424484 58-59 18.399577662394567 25.95672955776658 32.01057589914145 23.633116880697397 60-61 24.223906114601675 26.267024925534248 31.783313710001938 17.72575524986214 62-63 17.937362493493886 26.390200755507443 31.857092222360595 23.815344528638075 64-65 24.453749373439454 26.25679609960804 31.6680225956707 17.62143193128181 66-67 18.882701522577534 37.78240348789046 25.359561492900745 17.97533349663126 68-69 18.934389353666244 37.94932371363791 25.33289487439514 17.783392058300702 70-71 18.431420893912474 38.66920549996801 25.403492769467057 17.495880836652457 72-73 18.28949910660252 38.61420930464029 25.230875518886425 17.86541606987076 74-75 19.03719635250385 37.185972393804555 25.163856691029586 18.61297456266201 76-77 19.376275587740473 35.707876075283664 25.731498435200965 19.184349901774887 78-79 19.093580115730344 34.74698264726111 26.58075368537991 19.578683551628636 80-81 18.608218311416742 34.67442725333432 26.496274786416755 20.221079648832177 82-83 18.12014322012847 34.09420889099761 26.787425901080535 20.998221987793382 84-85 18.42509916384868 33.440645752044986 27.080056653804576 21.05419843030176 86-87 18.498579118114282 33.30831593293293 27.67265146758458 20.52045348136821 88-89 18.872607908585685 33.54098990654922 27.317445760276954 20.26895642458814 90-91 19.187056121062408 32.71143133336477 26.95699002909401 21.144522516478816 92-93 18.779229648530297 33.35158331355542 26.699252570675817 21.169934467238463 94-95 18.51848854689053 33.554864677981314 26.554139135853134 21.37250763927502 96-97 19.04147652462924 33.82313971858535 26.265285844127373 20.870097912658036 98-99 19.519566105044273 32.75304283850264 26.176401851123305 21.550989205329778 100-101 19.519843477031344 33.035196373464345 25.68398944475061 21.760970704753696 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 75.0 1 58.5 2 113.0 3 294.0 4 312.5 5 195.0 6 162.5 7 156.5 8 158.0 9 167.0 10 173.5 11 156.5 12 153.0 13 168.5 14 180.0 15 201.5 16 221.5 17 259.5 18 343.0 19 468.5 20 613.0 21 852.5 22 1225.0 23 1828.0 24 2642.0 25 3719.5 26 5406.0 27 7648.5 28 10381.0 29 13534.5 30 17461.0 31 22711.5 32 30744.5 33 48112.5 34 78113.0 35 128169.5 36 194868.0 37 273889.0 38 356906.5 39 398696.0 40 400016.0 41 367525.0 42 322707.0 43 310456.5 44 291750.0 45 260172.5 46 247473.0 47 218984.0 48 165814.5 49 121070.0 50 91547.0 51 73517.5 52 59695.5 53 46365.0 54 35358.5 55 25423.5 56 17196.0 57 11958.0 58 8647.0 59 6179.5 60 4408.0 61 3327.0 62 2365.0 63 1526.5 64 993.0 65 642.0 66 439.0 67 313.0 68 202.0 69 132.5 70 84.5 71 54.5 72 37.5 73 32.0 74 32.5 75 25.0 76 19.0 77 12.0 78 8.0 79 8.0 80 5.5 81 2.0 82 2.5 83 3.5 84 1.0 85 0.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17711652760504015 2 0.0 3 0.0 4 0.008599336275980798 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.002234976012321742 12-13 0.0 14-15 0.0 16-17 3.937814878852594E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.00249040184230137 40-41 0.006449502206985598 42-43 0.002873540587270811 44-45 0.0014048420648879521 46-47 0.007215779696924481 48-49 0.008907975820539515 50-51 0.005534226316225265 52-53 0.006907140152365764 54-55 0.01992321473841096 56-57 0.022328474637385784 58-59 0.02810748404067486 60-61 0.02654300083204964 62-63 0.01132387846243016 64-65 0.016294039404117083 66-67 0.02342467715771502 68-69 0.02733056380782016 70-71 0.02961875353472099 72-73 0.022977681955250674 74-75 0.03309893046819341 76-77 0.030385031024659875 78-79 0.02123227211705655 80-81 0.00966361056756258 82-83 0.00200083566817375 84-85 0.008141698330600631 86-87 0.027798844496116144 88-89 0.025106230538414237 90-91 0.021743123777015805 92-93 0.04558286790844772 94-95 0.04763691729120056 96-97 0.035280692765936074 98-99 0.04192176434540639 100-101 0.04225168937579674 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4698037.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.2749881150059 #Duplication Level Percentage of deduplicated Percentage of total 1 88.1371938304309 30.209012710279897 2 5.3051148137484985 3.6366549437994293 3 1.8594179874792314 1.911945882650365 4 0.921894400061858 1.2639167842164267 5 0.6046170725312597 1.036162148756929 6 0.3794454306449066 0.780329257538848 7 0.35626736801315007 0.8547741863090603 8 0.2651169688869302 0.7269504766148736 9 0.20009756291930492 0.6172507431810738 >10 1.5192814286941856 10.44117862466615 >50 0.19397839771790992 4.748687907952641 >100 0.203651887466976 14.217860300838911 >500 0.03679684237708266 8.835198998642847 >1k 0.016750607678604874 9.093804383647736 >5k 3.1283446542638803E-4 0.7700396928573618 >10k+ 6.256688390414562E-5 10.856232958047494 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 506241 10.775585632893057 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8857 0.18852554801079685 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT 7059 0.15025424448551597 TruSeq Adapter, Index 13 (97% over 38bp) TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6968 0.14831726527483713 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6861 0.14603971829085213 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 6163 0.13118244918037045 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4979 0.10598043395571384 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCCCATTCAGAAATCTCGTAT 4787 0.10189362067603981 TruSeq Adapter, Index 27 (97% over 37bp) GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 4744 0.10097834478527948 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005363942429572181 0.0 0.0 2 0.005810937632036529 0.0 0.0 3 0.006385645749490691 0.0 0.0 4 0.006726213522796861 0.0 0.0 5 0.007364778097745931 0.0 0.0 6 0.008194912045179721 0.0 0.0 7 0.009280471822593138 0.0 0.0 8 0.010280889656680014 0.0 2.1285485831635637E-5 9 0.011387734919925067 0.0 4.2570971663271274E-5 10-11 0.016964532207813604 0.0 4.2570971663271274E-5 12-13 0.02151962617578363 0.0 4.2570971663271274E-5 14-15 0.02598957820042711 0.0 4.2570971663271274E-5 16-17 0.029586825305973535 0.0 4.2570971663271274E-5 18-19 0.03390777892979557 0.0 6.385645749490691E-5 20-21 0.038846011642735045 0.0 6.385645749490691E-5 22-23 0.04381617258442196 0.0 6.385645749490691E-5 24-25 0.04996767798976466 0.0 6.385645749490691E-5 26-27 0.05641718019675026 0.0 7.449920041072472E-5 28-29 0.06400545589572837 0.0 8.514194332654255E-5 30-31 0.10190426341895562 0.0 8.514194332654255E-5 32-33 0.11091866666865331 0.0 8.514194332654255E-5 34-35 0.11881558191219013 0.0 1.064274291581782E-4 36-37 0.12682956732780096 0.0 1.1707017207399602E-4 38-39 0.13579075686291955 0.0 1.2771291498981383E-4 40-41 0.14489030205594378 0.0 1.2771291498981383E-4 42-43 0.15544790302843509 0.0 1.2771291498981383E-4 44-45 0.16637800000297998 0.0 1.2771291498981383E-4 46-47 0.17791473332372648 0.0 1.2771291498981383E-4 48-49 0.18967496424570518 0.0 1.2771291498981383E-4 50-51 0.20292517917589836 0.0 1.2771291498981383E-4 52-53 0.21594125376194356 0.0 1.2771291498981383E-4 54-55 0.2306069534999405 0.0 1.2771291498981383E-4 56-57 0.24670942353157288 0.0 1.2771291498981383E-4 58-59 0.26489787117470553 0.0 1.2771291498981383E-4 60-61 0.2841718785952516 0.0 1.2771291498981383E-4 62-63 0.30504229745317035 0.0 1.2771291498981383E-4 64-65 0.32743462854805105 1.0642742915817818E-5 1.2771291498981383E-4 66-67 0.35200872194067434 2.1285485831635637E-5 1.4899840082144945E-4 68-69 0.37818986951358624 2.1285485831635637E-5 1.702838866530851E-4 70-71 0.40643570921216665 2.1285485831635637E-5 1.702838866530851E-4 72-73 0.4381085121296405 2.1285485831635637E-5 1.9156937248472074E-4 74-75 0.4718353644298672 2.1285485831635637E-5 1.9156937248472074E-4 76-77 0.5089040380056606 2.1285485831635637E-5 1.9156937248472074E-4 78-79 0.550655518464414 2.1285485831635637E-5 1.9156937248472074E-4 80-81 0.59738780260777 2.1285485831635637E-5 1.9156937248472074E-4 82-83 0.6489518920349073 2.1285485831635637E-5 2.234976012321742E-4 84-85 0.7076679047014742 2.1285485831635637E-5 2.5542582997962766E-4 86-87 0.77287599054669 2.1285485831635637E-5 2.6606857289544544E-4 88-89 0.8452359996313354 2.1285485831635637E-5 2.9799680164289895E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 63455 0.0 86.61977 1 TCGGAAG 63655 0.0 86.46926 3 CGGAAGA 63730 0.0 85.92039 4 ATCGGAA 63975 0.0 85.68043 2 GAAGAGC 64290 0.0 85.15448 6 GGAAGAG 66700 0.0 82.21297 5 AGAGCAC 82070 0.0 66.71782 8 GAGCACA 82210 0.0 66.7024 9 AAGAGCA 82885 0.0 65.94147 7 TCGGACG 280 0.0 55.971478 3 ATCGGAC 280 0.0 55.971478 2 CTCGGAA 420 0.0 52.013893 2 CGACGCG 40 5.347564E-4 47.57602 1 CGATGCG 50 2.8017343E-5 47.57602 1 TTGTGTT 40405 0.0 46.86364 1 GCCGTCT 59650 0.0 46.03428 50-51 ATGCCGT 59520 0.0 45.988228 48-49 CGTCTTC 59825 0.0 45.933342 52-53 TCGTATG 59935 0.0 45.886333 44-45 TCTCGTA 58815 0.0 45.8628 42-43 >>END_MODULE