FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_53115004-1.3410000003588d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_53115004-1.3410000003588d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4698037
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT50624110.775585632893057TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC88570.18852554801079685No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT70590.15025424448551597TruSeq Adapter, Index 13 (97% over 38bp)
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG69680.14831726527483713No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA68610.14603971829085213No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC61630.13118244918037045No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG49790.10598043395571384No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCATTCAGAAATCTCGTAT47870.10189362067603981TruSeq Adapter, Index 27 (97% over 37bp)
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT47440.10097834478527948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA634550.086.619771
TCGGAAG636550.086.469263
CGGAAGA637300.085.920394
ATCGGAA639750.085.680432
GAAGAGC642900.085.154486
GGAAGAG667000.082.212975
AGAGCAC820700.066.717828
GAGCACA822100.066.70249
AAGAGCA828850.065.941477
TCGGACG2800.055.9714783
ATCGGAC2800.055.9714782
CTCGGAA4200.052.0138932
CGACGCG405.347564E-447.576021
CGATGCG502.8017343E-547.576021
TTGTGTT404050.046.863641
GCCGTCT596500.046.0342850-51
ATGCCGT595200.045.98822848-49
CGTCTTC598250.045.93334252-53
TCGTATG599350.045.88633344-45
TCTCGTA588150.045.862842-43
Produced by FastQC (version 0.11.2)