##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_52370180-2.341000000358c0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5321893 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.670753433035955 33.0 33.0 33.0 33.0 33.0 2 32.679308659531486 33.0 33.0 33.0 33.0 33.0 3 32.74386877000345 33.0 33.0 33.0 33.0 33.0 4 36.683792966149454 37.0 37.0 37.0 37.0 37.0 5 36.65525612784774 37.0 37.0 37.0 37.0 37.0 6 36.56851950988116 37.0 37.0 37.0 37.0 37.0 7 36.46938673889159 37.0 37.0 37.0 37.0 37.0 8 36.41863111490592 37.0 37.0 37.0 37.0 37.0 9 36.492642185778635 37.0 37.0 37.0 37.0 37.0 10-11 36.423410523285604 37.0 37.0 37.0 37.0 37.0 12-13 36.41283214450196 37.0 37.0 37.0 37.0 37.0 14-15 38.31488090797767 40.0 37.0 40.0 37.0 40.0 16-17 38.356664724375335 40.0 37.0 40.0 37.0 40.0 18-19 38.314828483022865 40.0 37.0 40.0 37.0 40.0 20-21 38.342740261782794 40.0 37.0 40.0 37.0 40.0 22-23 38.15384760648138 40.0 37.0 40.0 37.0 40.0 24-25 38.20915508823646 40.0 37.0 40.0 37.0 40.0 26-27 38.17162793389495 40.0 37.0 40.0 37.0 40.0 28-29 37.991290974846734 40.0 37.0 40.0 35.0 40.0 30-31 38.02168495307967 40.0 37.0 40.0 37.0 40.0 32-33 37.89035339492921 40.0 37.0 40.0 33.0 40.0 34-35 37.72512290269647 40.0 37.0 40.0 33.0 40.0 36-37 37.41538433786624 40.0 37.0 40.0 33.0 40.0 38-39 37.64097220669412 40.0 37.0 40.0 33.0 40.0 40-41 37.41002177232801 40.0 37.0 40.0 33.0 40.0 42-43 37.229204345145604 40.0 37.0 40.0 33.0 40.0 44-45 37.30703971312464 40.0 37.0 40.0 33.0 40.0 46-47 37.36238599686239 40.0 37.0 40.0 33.0 40.0 48-49 37.04772051974739 38.5 37.0 40.0 33.0 40.0 50-51 37.01032395803523 37.0 37.0 40.0 33.0 40.0 52-53 36.98106951793281 37.0 37.0 40.0 33.0 40.0 54-55 36.614693587413356 37.0 37.0 40.0 33.0 40.0 56-57 36.57676958180106 37.0 37.0 40.0 33.0 40.0 58-59 36.34762987530941 37.0 37.0 40.0 33.0 40.0 60-61 36.08271342546722 37.0 37.0 40.0 33.0 40.0 62-63 35.86331602307675 37.0 37.0 40.0 33.0 40.0 64-65 35.6285907101101 37.0 35.0 40.0 30.0 40.0 66-67 35.02776737525539 37.0 33.0 37.0 27.0 40.0 68-69 34.88833954384276 37.0 33.0 37.0 27.0 40.0 70-71 34.369041805237345 37.0 33.0 37.0 27.0 40.0 72-73 33.63104622734805 37.0 33.0 37.0 24.5 40.0 74-75 32.747452739091145 37.0 33.0 37.0 22.0 38.5 76-77 30.316235407213185 33.0 30.0 35.0 18.5 37.0 78-79 32.224609645477656 35.0 33.0 37.0 18.5 37.0 80-81 32.43890811032841 37.0 33.0 37.0 15.0 37.0 82-83 32.36037815867399 37.0 33.0 37.0 15.0 37.0 84-85 32.19791275397682 37.0 33.0 37.0 15.0 37.0 86-87 32.04893362192739 37.0 33.0 37.0 15.0 37.0 88-89 31.84076305179379 37.0 33.0 37.0 15.0 37.0 90-91 31.58964263280002 37.0 33.0 37.0 15.0 37.0 92-93 31.568572592496693 33.0 33.0 37.0 15.0 37.0 94-95 31.506325380837232 33.0 33.0 37.0 15.0 37.0 96-97 31.359503469911928 33.0 33.0 37.0 15.0 37.0 98-99 31.212986525659197 33.0 33.0 37.0 15.0 37.0 100-101 29.82349457232605 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.12057681819651123 2306 2 0.16380208318516054 2306 3 0.12262189619315933 2306 4 0.12272738737644318 2306 5 0.21140002864812857 2306 6 0.15225149930537185 2306 7 0.15197861871367735 2306 8 0.13751914351759353 2306 9 0.12519311702546787 2306 10-11 0.17681871535190652 2306 12-13 0.1771003103399451 2306 14-15 0.3485931356199856 2306 16-17 0.40759391308611015 2306 18-19 0.3718962232284113 2306 20-21 0.35780251366202265 2306 22-23 0.38737955461382967 2306 24-25 0.345841685677172 2306 26-27 0.4017279782507259 2306 28-29 0.43785827343912587 2306 30-31 0.43431868183964184 2306 32-33 0.43061399620015806 2306 34-35 0.4323490244054753 2306 36-37 0.5010728471651902 2306 38-39 0.41294691578082876 2306 40-41 0.48052421431991377 2306 42-43 0.49239752863186936 2306 44-45 0.5078253185609398 2306 46-47 0.4559221891276124 2306 48-49 0.47245742491787013 2306 50-51 0.42706245184485425 2306 52-53 0.29803361403854467 2306 54-55 0.4509919045694417 2306 56-57 0.49649466986608815 2306 58-59 0.48151095818714396 2306 60-61 0.4594819321236656 2306 62-63 0.45412097420375375 2306 64-65 0.31615940959229505 2306 66-67 0.2997872650961426 2306 68-69 0.436881866707985 2306 70-71 0.3876089469851749 2306 72-73 0.32291160349880244 2306 74-75 0.4012129331221388 2306 76-77 0.0941914513932467 2306 78-79 0.5558696630311175 2306 80-81 0.692398124517986 2306 82-83 0.639771108553127 2306 84-85 0.6229092570643289 2306 86-87 0.5989713578651816 2306 88-89 0.7327742090750355 2306 90-91 0.7798402305906222 2306 92-93 0.5882563605565139 2306 94-95 0.5080658972224761 2306 96-97 0.5766144486193809 2306 98-99 0.5455138735893037 2306 100-101 0.4217616407568876 2307 1 0.14247511623316456 2307 2 0.1616467827425936 2307 3 0.11308919354664937 2307 4 0.09990273712545417 2307 5 0.1545537474231935 2307 6 0.15326417492244815 2307 7 0.1564154559727129 2307 8 0.1806056599877266 2307 9 0.1652202578458528 2307 10-11 0.14790919341648134 2307 12-13 0.16357399005597983 2307 14-15 0.285276776128498 2307 16-17 0.2823307659390508 2307 18-19 0.2733232511786312 2307 20-21 0.23417208549794566 2307 22-23 0.25398019470650723 2307 24-25 0.3433081402172249 2307 26-27 0.3688099873397519 2307 28-29 0.36514472976134726 2307 30-31 0.28610325575475315 2307 32-33 0.31740317403008333 2307 34-35 0.41529458311281786 2307 36-37 0.3418655744649186 2307 38-39 0.40054790487995007 2307 40-41 0.4489785798707473 2307 42-43 0.33012623923870166 2307 44-45 0.35809784061518 2307 46-47 0.39371643126383304 2307 48-49 0.5219518296762331 2307 50-51 0.2933131572834142 2307 52-53 0.26397253419774813 2307 54-55 0.41511478454092554 2307 56-57 0.39410072698433396 2307 58-59 0.4113588062282503 2307 60-61 0.3740941636857329 2307 62-63 0.29593055405938884 2307 64-65 0.3868499225575093 2307 66-67 0.185709496064149 2307 68-69 0.24984504281515285 2307 70-71 0.32022239264412633 2307 72-73 0.010828369174383567 2307 74-75 0.062241351157602764 2307 76-77 0.015634834379554263 2307 78-79 0.3927755166860294 2307 80-81 0.46570590736171624 2307 82-83 0.29253382872420985 2307 84-85 0.33226485750392243 2307 86-87 0.27223588618667804 2307 88-89 0.4701812025701493 2307 90-91 0.5368523826980294 2307 92-93 0.2870809699663184 2307 94-95 0.34535556514655497 2307 96-97 0.3788712022653833 2307 98-99 0.21884337651501795 2307 100-101 0.26731097318924313 2201 1 0.15285041953987388 2201 2 0.15721584811515044 2201 3 0.0979922682353731 2201 4 0.14046281399267002 2201 5 0.1574518762200583 2201 6 0.11201523760288268 2201 7 0.152392324961923 2201 8 0.15844866223706333 2201 9 0.1401036950857204 2201 10-11 0.18181185410261946 2201 12-13 0.1600989696879438 2201 14-15 0.31573469176938573 2201 16-17 0.3014761162693702 2201 18-19 0.24874035324908306 2201 20-21 0.2604990523423183 2201 22-23 0.31653963839330146 2201 24-25 0.3248878478915529 2201 26-27 0.34727189024618355 2201 28-29 0.38541220421273437 2201 30-31 0.3556638816735287 2201 32-33 0.3529057775971509 2201 34-35 0.35015221714338907 2201 36-37 0.39631477391233005 2201 38-39 0.32502818773005515 2201 40-41 0.36947032417212 2201 42-43 0.3647879969121348 2201 44-45 0.4115109266391599 2201 46-47 0.44911019864308344 2201 48-49 0.5744039094304085 2201 50-51 0.4102612497720344 2201 52-53 0.3257040280607697 2201 54-55 0.47494612830033134 2201 56-57 0.4450311811916592 2201 58-59 0.525448288348656 2201 60-61 0.33410428128902936 2201 62-63 0.4632173205885053 2201 64-65 0.4458353284128762 2201 66-67 0.5080315413697605 2201 68-69 0.33779092581403347 2201 70-71 0.45916370833106157 2201 72-73 0.13831160981837343 2201 74-75 0.14825227019906606 2201 76-77 0.048459870687803885 2201 78-79 0.41334718341173726 2201 80-81 0.39626654877792333 2201 82-83 0.2984384575830745 2201 84-85 0.39804411221071945 2201 86-87 0.4071509713782291 2201 88-89 0.47368373086401405 2201 90-91 0.507427813083595 2201 92-93 0.4615527845817198 2201 94-95 0.25423127138626 2201 96-97 0.3137227480226308 2201 98-99 0.292074954506905 2201 100-101 0.28510118457409206 1101 1 0.11364016960991563 1101 2 0.10221075330684215 1101 3 0.10576512482891332 1101 4 0.08269165305986093 1101 5 0.12972876248609566 1101 6 0.13897603444863904 1101 7 0.1451783581436601 1101 8 0.13971797646491524 1101 9 0.14331183300502204 1101 10-11 0.11844869105362221 1101 12-13 0.12244891023546955 1101 14-15 0.24313881259016767 1101 16-17 0.20044009365290805 1101 18-19 0.22436559553292312 1101 20-21 0.2213223504076467 1101 22-23 0.18311153936463143 1101 24-25 0.23581552039632925 1101 26-27 0.2528568488485803 1101 28-29 0.21698441562040927 1101 30-31 0.2394418346405942 1101 32-33 0.18502224392367594 1101 34-35 0.16038226873843087 1101 36-37 0.24495086268564137 1101 38-39 0.2591643685907954 1101 40-41 0.30948147379357493 1101 42-43 0.27370847668499465 1101 44-45 0.2867836280145255 1101 46-47 0.3071441087271509 1101 48-49 0.4006139981421555 1101 50-51 0.23391451689285958 1101 52-53 0.25856134160200384 1101 54-55 0.5021838395861806 1101 56-57 0.24114313549543454 1101 58-59 0.3686439409875959 1101 60-61 0.35186935349548065 1101 62-63 0.31706939219025543 1101 64-65 0.24450183473091158 1101 66-67 0.2192858242174509 1101 68-69 0.34280987286611975 1101 70-71 0.3573574122645198 1101 72-73 0.09870222744061863 1101 74-75 0.061104230669819515 1101 76-77 -0.07775347890995832 1101 78-79 0.21484236486569586 1101 80-81 0.25452311215884293 1101 82-83 0.25285364506836316 1101 84-85 0.22648261216141208 1101 86-87 0.145516338786166 1101 88-89 0.22796869160555033 1101 90-91 0.3223894363683044 1101 92-93 0.14971879446246206 1101 94-95 0.14464006649148686 1101 96-97 0.25301905925620005 1101 98-99 0.19139898588028004 1101 100-101 0.2082239707585778 2304 1 0.11619896807724217 2304 2 0.12204877679587867 2304 3 0.08559752945076582 2304 4 0.08417487157636572 2304 5 0.13793219254734623 2304 6 0.043997988828309076 2304 7 0.0909392264268547 2304 8 0.1209512308002445 2304 9 0.12462518423391344 2304 10-11 0.07021965415568587 2304 12-13 0.12032564164800874 2304 14-15 0.24313318115498106 2304 16-17 0.23211648151406905 2304 18-19 0.24160907423429734 2304 20-21 0.1890317494034761 2304 22-23 0.22789582977769385 2304 24-25 0.2791092690102275 2304 26-27 0.26991424849639856 2304 28-29 0.3782597283173246 2304 30-31 0.28120632231727427 2304 32-33 0.33725878895069883 2304 34-35 0.46968297306903395 2304 36-37 0.4571285246529655 2304 38-39 0.3112234823218074 2304 40-41 0.36807467167727026 2304 42-43 0.4336854897949678 2304 44-45 0.49969683275842414 2304 46-47 0.4336093936603689 2304 48-49 0.37631462118674364 2304 50-51 0.3048687633430305 2304 52-53 0.3272055153336595 2304 54-55 0.4642272897098394 2304 56-57 0.4918001282104356 2304 58-59 0.4268247389634112 2304 60-61 0.42911707921741993 2304 62-63 0.44831032751314837 2304 64-65 0.398706433423726 2304 66-67 0.4755569227639853 2304 68-69 0.3691112371139198 2304 70-71 0.5052422355418713 2304 72-73 0.2674070466536662 2304 74-75 0.37172779054508 2304 76-77 0.417931125722685 2304 78-79 0.6041742707309155 2304 80-81 0.7416279231736809 2304 82-83 0.6672214374355647 2304 84-85 0.6584157209591908 2304 86-87 0.6037420142248564 2304 88-89 0.7573697137907978 2304 90-91 0.823889210820866 2304 92-93 0.626533692426662 2304 94-95 0.6723389934348099 2304 96-97 0.5987086234188865 2304 98-99 0.42567974091217664 2304 100-101 0.5696902543651952 2202 1 0.13548632741522937 2202 2 0.14191181431854005 2202 3 0.10662850388375489 2202 4 0.0984040395233734 2202 5 0.15840159126663877 2202 6 0.07910264358089591 2202 7 0.13617188836241922 2202 8 0.19766038270017106 2202 9 0.13047266344200636 2202 10-11 0.15411680848429654 2202 12-13 0.18028533831782312 2202 14-15 0.25636609738558747 2202 16-17 0.25533953412502797 2202 18-19 0.1746784090103759 2202 20-21 0.22334427782244148 2202 22-23 0.14421652032838495 2202 24-25 0.16794399579985253 2202 26-27 0.16004942135665345 2202 28-29 0.16234422924214442 2202 30-31 0.21832946419281285 2202 32-33 0.27089494844324946 2202 34-35 0.35315605753445567 2202 36-37 0.24608817770874936 2202 38-39 0.2851327395442098 2202 40-41 0.28486371745453454 2202 42-43 0.37917446076153993 2202 44-45 0.2142473207760105 2202 46-47 0.31553325635690754 2202 48-49 0.40571219065966346 2202 50-51 0.4154934117950049 2202 52-53 0.3864619132147453 2202 54-55 0.4973329529918544 2202 56-57 0.43391833827615045 2202 58-59 0.3451957620891193 2202 60-61 0.3929743834773731 2202 62-63 0.29575017934799774 2202 64-65 0.2996102621409662 2202 66-67 0.24644799888254454 2202 68-69 0.3811913326610892 2202 70-71 0.4047561823900594 2202 72-73 0.1576247843001184 2202 74-75 0.18717765125951757 2202 76-77 0.09833797705452696 2202 78-79 0.35062535912628334 2202 80-81 0.3672629398704643 2202 82-83 0.2542329400056431 2202 84-85 0.31280578863843544 2202 86-87 0.31368554486722644 2202 88-89 0.3188946946285576 2202 90-91 0.4374520167386109 2202 92-93 0.3314471239804746 2202 94-95 0.29064070269033593 2202 96-97 0.2333196601478491 2202 98-99 0.14984631191031994 2202 100-101 0.286317567778692 1102 1 0.08901939611448029 1102 2 0.0911882602602816 1102 3 0.05147206489153433 1102 4 0.022438305586817364 1102 5 0.1026299755178357 1102 6 0.07437716343402201 1102 7 0.09916988776495828 1102 8 0.07644031419381037 1102 9 0.060073767774589726 1102 10-11 0.06951790547044823 1102 12-13 0.0629016922446155 1102 14-15 0.15002270889713287 1102 16-17 0.06357244444073729 1102 18-19 0.09354956423181449 1102 20-21 0.11025744663512427 1102 22-23 0.09714927487186031 1102 24-25 0.09829985041985623 1102 26-27 0.1135777462096712 1102 28-29 0.08302572758031346 1102 30-31 0.05153052110434686 1102 32-33 0.06246575169740254 1102 34-35 0.05179846013850664 1102 36-37 0.15531026265441739 1102 38-39 0.10040651866098926 1102 40-41 0.107703234964454 1102 42-43 0.10300322688800634 1102 44-45 0.03498753400362631 1102 46-47 0.15329645713180184 1102 48-49 0.1656320083427687 1102 50-51 0.10088762280546604 1102 52-53 0.09707854533112226 1102 54-55 0.17188214397893375 1102 56-57 0.26167913927415754 1102 58-59 0.12962457427876473 1102 60-61 0.10522100551068547 1102 62-63 0.11444461976911668 1102 64-65 0.08267617601291732 1102 66-67 0.21343276582652493 1102 68-69 0.019831696716906322 1102 70-71 0.13585167227182637 1102 72-73 0.07795195214210793 1102 74-75 0.11759041027352168 1102 76-77 0.0010805338292279032 1102 78-79 0.27273473341906396 1102 80-81 0.3317492013436123 1102 82-83 0.2430011006676125 1102 84-85 0.18921830149469798 1102 86-87 0.21088147912117705 1102 88-89 0.3954089738668394 1102 90-91 0.42993896115152097 1102 92-93 0.24763350160386466 1102 94-95 0.23360180541436293 1102 96-97 0.08249707721149235 1102 98-99 -0.032277648186358476 1102 100-101 0.049767590444698584 2305 1 0.09963392467544452 2305 2 0.14257916661865266 2305 3 0.1014362966472433 2305 4 0.061181636903050673 2305 5 0.11259211844780737 2305 6 0.019848383983564588 2305 7 0.021226563466647974 2305 8 0.0786772777363467 2305 9 0.04828787473041274 2305 10-11 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1213 98-99 -1.4583798399043246 1213 100-101 -1.538197260250037 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 6.0 9 18.0 10 30.0 11 23.0 12 37.0 13 93.0 14 271.0 15 726.0 16 1521.0 17 2701.0 18 4204.0 19 5939.0 20 8433.0 21 11459.0 22 15854.0 23 21601.0 24 28931.0 25 38951.0 26 54982.0 27 85181.0 28 150781.0 29 206760.0 30 130197.0 31 94972.0 32 122702.0 33 176039.0 34 271570.0 35 483170.0 36 1014118.0 37 1776329.0 38 613945.0 39 348.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.618435123937616 24.44408928907244 23.11156013793833 12.825915449051614 2 26.97791932306794 29.41306786889552 32.153803167406785 11.455209640629754 3 34.62874958215056 18.195875039201276 32.41899076137006 14.756384617278101 4 25.515302683304185 18.498974045676437 33.764430213401134 22.22129305761825 5 36.518096098512316 17.583104357791484 31.80063935896494 14.09816018473126 6 30.14639715604955 18.08384723255428 37.49462832116316 14.275127290233005 7 30.05795118391144 26.18932774484568 28.485014636709156 15.267706434533727 8 25.412480108111907 33.67019968270689 26.371142749393872 14.546177459787335 9 36.14852835259935 23.354359059830777 24.566859950021545 15.930252637548332 10-11 26.19703504883898 30.832556963148004 28.190477461087227 14.779930526925797 12-13 22.487740734358997 29.141764030205042 27.569635466177168 20.800859769258796 14-15 21.395103208576348 29.99144289447383 28.19309783191808 20.420356065031747 16-17 24.498189643849607 26.675211264588594 28.877487114048584 19.949111977513216 18-19 19.157919935631927 27.002872849942683 33.27099022847697 20.56821698594842 20-21 23.453149095631947 27.11494763235563 32.95824436906191 16.47365890295051 22-23 18.979242912249457 35.76807162413825 28.746904531902462 16.505780931709825 24-25 19.00069204698403 27.14674083075327 32.96922730314195 20.883339819120753 26-27 19.08165947718227 27.254672350609077 28.379779525819103 25.283888646389546 28-29 22.874003667491998 31.596163244920554 28.33311379992044 17.196719287667 30-31 18.130522353606132 27.925627215729442 32.3488559428008 21.594994487863623 32-33 17.938569978765077 32.24220216377894 28.519447873153407 21.299779984302578 34-35 22.4852885993762 32.1890913627914 28.76600487833934 16.55961515949306 36-37 18.137446581507746 32.22919927176289 33.161546088957444 16.471808057771923 38-39 18.49692311421542 27.961271206967147 32.521157876401055 21.020647802416374 40-41 22.658695088839334 27.808948594710625 32.28684451007249 17.24551180637755 42-43 18.413316510560566 32.098962851443076 32.535707913621344 16.952012724375017 44-45 17.980373274205746 28.0233942303627 32.810586593884615 21.18564590154693 46-47 22.38112982686782 27.926957623740943 28.45658804470359 21.235324504687647 48-49 18.55634233904615 32.26678302580939 32.00825413272741 17.168620502417053 50-51 22.796017366940987 27.865005012585485 31.692149266997454 17.646828353476067 52-53 18.317062390821324 27.738167361701997 27.7811341503381 26.163636097138582 54-55 22.647250740660834 27.741497529376474 31.89824737207297 17.71300435788972 56-57 18.465011638808733 27.64117229881766 31.806034326004863 22.087781736368743 58-59 18.503118190514435 27.70205508964569 32.25890515195687 21.53592156788301 60-61 22.46220513464362 28.089099200761424 31.996250773414246 17.45244489118071 62-63 18.219085678623486 28.240083144213322 31.93346470960929 21.607366467553906 64-65 22.52898398268549 27.9799770178472 31.99014561972364 17.500893379743662 66-67 18.583112606990333 35.96589240006608 27.637359508379646 17.813635484563942 68-69 18.450884671621743 36.191868728953594 27.63079116032255 17.72645543910211 70-71 18.116560919823677 36.658549499277406 27.661547200018944 17.563342380879973 72-73 17.98977473442614 36.54000701405932 27.63294104468888 17.837277206825664 74-75 18.482995465607864 35.48593218185386 27.615667975131892 18.415404377406382 76-77 18.92971369859293 34.57011934934463 27.974154899749347 18.52601205231309 78-79 19.021119332913848 33.89085162189531 28.386870274687396 18.701158770503447 80-81 18.579216642786747 33.9329199283687 28.259120480420957 19.2287429484236 82-83 18.170727445054347 33.59077109942418 28.388935406114168 19.849566049407304 84-85 18.235006438513253 32.97956353410251 28.980986067348585 19.80444396003565 86-87 18.130479972784563 32.843204880735115 29.55736386173506 19.46895128474526 88-89 18.49395294218476 33.14576690917046 29.033480033469882 19.326800115174894 90-91 18.81773781379498 32.45567247426182 28.81939922090531 19.907190491037895 92-93 18.442574260031453 33.05179996007232 28.77015793239289 19.735467847503337 94-95 18.20048175114011 33.231269591054804 28.62042974863576 19.947818909169314 96-97 18.660560934750276 33.35665666535874 28.30322524558811 19.679557154302874 98-99 18.941195113615986 32.53199109283461 28.18851670431873 20.338297089230682 100-101 18.83543756996878 32.64809554749738 27.917649280276162 20.598817602257686 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 424.0 1 305.5 2 259.5 3 578.0 4 678.0 5 454.0 6 384.5 7 374.5 8 358.5 9 362.5 10 363.5 11 372.0 12 377.5 13 413.5 14 447.5 15 488.5 16 587.5 17 763.5 18 1130.5 19 1690.0 20 2584.0 21 4076.5 22 6379.5 23 9828.0 24 14392.5 25 20230.0 26 28444.0 27 39033.0 28 50436.0 29 62749.5 30 75925.0 31 89424.5 32 106073.5 33 127379.5 34 154287.0 35 193898.0 36 251562.0 37 323322.0 38 388921.5 39 410064.0 40 407659.5 41 379005.5 42 327641.5 43 303587.0 44 276229.0 45 246111.0 46 239476.0 47 210921.0 48 148841.0 49 100739.0 50 74337.0 51 57547.0 52 45074.0 53 33517.5 54 25212.5 55 19410.5 56 14541.0 57 10910.5 58 8414.5 59 6465.0 60 4793.5 61 3462.0 62 2567.5 63 1901.0 64 1327.0 65 883.5 66 554.0 67 364.0 68 260.5 69 158.0 70 107.0 71 86.0 72 55.0 73 33.5 74 25.0 75 17.5 76 10.5 77 7.0 78 6.0 79 6.0 80 5.5 81 3.5 82 2.5 83 2.0 84 1.0 85 1.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18158952087161467 2 0.0 3 0.0 4 0.009507895029080818 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0022360464594083345 12-13 0.0 14-15 0.0 16-17 2.2548367657899173E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0021796755402635867 40-41 0.006012898042106446 42-43 0.002602457433849196 44-45 0.0012777408339476198 46-47 0.00661418784631709 48-49 0.008220759041942407 50-51 0.005411608237895801 52-53 0.0064074944761196816 54-55 0.01820780688375358 56-57 0.020979377075037022 58-59 0.026672839908656563 60-61 0.024972317181123336 62-63 0.010080999373719089 64-65 0.015173172403127985 66-67 0.022585948270662338 68-69 0.025545421525761607 70-71 0.027847234057505478 72-73 0.021627642645201624 74-75 0.0312858601253351 76-77 0.028251225644709506 78-79 0.020312321198490836 80-81 0.008953580990824129 82-83 0.0016723372679608554 84-85 0.007525517705823848 86-87 0.025986993725728796 88-89 0.024220704925860027 90-91 0.020706917632504075 92-93 0.042747947018100516 94-95 0.043997502392475754 96-97 0.03298638285286833 98-99 0.040042142899152616 100-101 0.03963815131194859 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5321893.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.84973606350144 #Duplication Level Percentage of deduplicated Percentage of total 1 93.3616499334077 49.34138557433614 2 3.4069313170505806 3.60110841785201 3 1.0197686452286774 1.6168351123851006 4 0.5022249634722067 1.0616982705603109 5 0.27720884971800663 0.7325207271031743 6 0.17260005421144872 0.5473120385772662 7 0.17881159745920325 0.6615102011568367 8 0.1125586595977445 0.475895636112184 9 0.10014368944541537 0.4763310800053911 >10 0.6639062356941916 7.050858750488653 >50 0.08914305802167274 3.3255349842161657 >100 0.08918983726452388 9.869935926384086 >500 0.017423373456029 6.431233416917341 >1k 0.008154650282174356 6.426688323229973 >5k 2.494942838536823E-4 0.8675042129987771 >10k+ 3.564140656608922E-5 7.5136473276766065 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 398890 7.495265312549501 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8444 0.15866534708608382 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 7614 0.14306939278937025 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6614 0.1242790864077876 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6395 0.120164009310221 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6063 0.11392562759153556 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5672 0.10657861779633676 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0010898377701317934 0.0 0.0 2 0.0011649989956581239 0.0 0.0 3 0.001315321446710785 0.0 0.0 4 0.0013904826722371155 0.0 0.0 5 0.0016535469615792727 0.0 0.0 6 0.001897820944539847 0.0 0.0 7 0.0022172561530267518 0.0 0.0 8 0.002687013812566318 0.0 0.0 9 0.003006449021053223 0.0 0.0 10-11 0.005505559769803714 0.0 0.0 12-13 0.007741606229212049 0.0 0.0 14-15 0.01009039452690988 0.0 9.395153190791321E-6 16-17 0.01123660321618642 0.0 1.8790306381582642E-5 18-19 0.012598900428851163 0.0 1.8790306381582642E-5 20-21 0.014337003769147557 0.0 2.8185459572373963E-5 22-23 0.015586559143522805 0.0 3.7580612763165285E-5 24-25 0.017409218862536317 0.0 5.6370919144747934E-5 26-27 0.019241273734740626 0.0 5.6370919144747934E-5 28-29 0.021364578355859466 0.0 5.6370919144747934E-5 30-31 0.03821008802694831 0.0 7.516122552633057E-5 32-33 0.04123532735438311 0.0 1.1274183828949587E-4 34-35 0.04321770467764008 0.0 1.1274183828949587E-4 36-37 0.04553830751576554 0.0 1.3153214467107852E-4 38-39 0.04827229709428581 0.0 1.3153214467107852E-4 40-41 0.0513163267281022 0.0 1.4092729786186983E-4 42-43 0.0548864849406029 0.0 1.5971760424345245E-4 44-45 0.05846603830629439 0.0 1.691127574342438E-4 46-47 0.06226168019537409 0.0 1.691127574342438E-4 48-49 0.06678074888014471 0.0 1.8790306381582644E-4 50-51 0.07174138976488253 0.0 2.6306428934215703E-4 52-53 0.07743485259850208 9.395153190791321E-6 2.6306428934215703E-4 54-55 0.08362625855123355 1.8790306381582642E-5 2.6306428934215703E-4 56-57 0.0908229458953797 1.8790306381582642E-5 2.6306428934215703E-4 58-59 0.09829209268205881 1.8790306381582642E-5 2.8185459572373965E-4 60-61 0.10695442392396841 1.8790306381582642E-5 3.006449021053223E-4 62-63 0.11743941488489153 1.8790306381582642E-5 3.476206680592789E-4 64-65 0.12873238902022272 1.8790306381582642E-5 3.5701582125007025E-4 66-67 0.14157556343203442 1.8790306381582642E-5 3.6641097444086156E-4 68-69 0.1556682932182214 1.8790306381582642E-5 3.7580612763165287E-4 70-71 0.17204404522977068 1.8790306381582642E-5 3.7580612763165287E-4 72-73 0.19018608604118872 1.8790306381582642E-5 3.852012808224442E-4 74-75 0.21033868963543612 1.8790306381582642E-5 3.945964340132355E-4 76-77 0.2322200014167891 1.8790306381582642E-5 4.321770467764008E-4 78-79 0.2572205040574848 1.8790306381582642E-5 4.321770467764008E-4 80-81 0.2861669710383129 1.8790306381582642E-5 4.5096735315798347E-4 82-83 0.31938823272095096 1.8790306381582642E-5 4.603625063487748E-4 84-85 0.3554186452076357 1.8790306381582642E-5 5.073382723027313E-4 86-87 0.39749014119599924 1.8790306381582642E-5 5.073382723027313E-4 88-89 0.4448041326648243 1.8790306381582642E-5 5.073382723027313E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 49635 0.0 88.11631 1 TCGGAAG 49790 0.0 87.82542 3 ATCGGAA 49725 0.0 87.56771 2 CGGAAGA 49870 0.0 87.4274 4 GAAGAGC 51085 0.0 85.416794 6 GGAAGAG 53370 0.0 81.81312 5 TCGGACG 235 0.0 70.73664 3 AGAGCAC 68550 0.0 63.626793 8 GAGCACA 68960 0.0 63.35181 9 AAGAGCA 70385 0.0 61.927505 7 ATCGGAC 325 0.0 51.148026 2 CGACGGG 120 0.0 47.58626 1 TCGCGAA 25 2.832372E-5 47.48924 30-31 ATGCCGT 46405 0.0 46.444557 48-49 GCCGTCT 46595 0.0 46.410698 50-51 TCTCGTA 46485 0.0 46.292118 42-43 TATGCCG 46450 0.0 46.281967 48-49 TCGTATG 47000 0.0 46.22552 44-45 TGCCGTC 46610 0.0 46.197033 50-51 CGTCTTC 46835 0.0 46.170403 52-53 >>END_MODULE