FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_52370180-2.341000000358c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_52370180-2.341000000358c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5321893
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT3988907.495265312549501TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC84440.15866534708608382No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC76140.14306939278937025No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG66140.1242790864077876No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA63950.120164009310221No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG60630.11392562759153556No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA56720.10657861779633676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA496350.088.116311
TCGGAAG497900.087.825423
ATCGGAA497250.087.567712
CGGAAGA498700.087.42744
GAAGAGC510850.085.4167946
GGAAGAG533700.081.813125
TCGGACG2350.070.736643
AGAGCAC685500.063.6267938
GAGCACA689600.063.351819
AAGAGCA703850.061.9275057
ATCGGAC3250.051.1480262
CGACGGG1200.047.586261
TCGCGAA252.832372E-547.4892430-31
ATGCCGT464050.046.44455748-49
GCCGTCT465950.046.41069850-51
TCTCGTA464850.046.29211842-43
TATGCCG464500.046.28196748-49
TCGTATG470000.046.2255244-45
TGCCGTC466100.046.19703350-51
CGTCTTC468350.046.17040352-53
Produced by FastQC (version 0.11.2)