FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_52260024-1.34100000035820.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_52260024-1.34100000035820.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6635557
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1668572.514589204794714TruSeq Adapter, Index 7 (97% over 38bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC68130.10267412366437362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA276050.065.601074
TCGGAAG278300.065.2584153
GAAGAGC278550.064.941646
GATCGGA284700.063.672551
ATCGGAA290850.062.1322982
GGAAGAG307550.058.9879235
CGAGCGT558.1033795E-751.9042931
TTGTGTT612950.045.5723841
GCCGTCT202450.044.46381850-51
ATGCCGT203250.044.24072648-49
TGCCGTC202400.044.19316550-51
TATGCCG202600.044.17165448-49
TCGTATG206050.044.0162744-45
CGTCTTC204800.044.01275652-53
CGTATGC204900.043.89291446-47
TCTCGTA203350.043.7937242-43
CTCGTAT203650.043.5554444-45
CATCTCG204200.043.4702340-41
ATCTCGT203500.043.3646742-43
CTGCTTG212200.042.86009258-59
Produced by FastQC (version 0.11.2)