##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_52150223-1.3410000003573e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3217964 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.664590405610504 33.0 33.0 33.0 33.0 33.0 2 32.67819932106139 33.0 33.0 33.0 33.0 33.0 3 32.73661762530594 33.0 33.0 33.0 33.0 33.0 4 36.68235474355835 37.0 37.0 37.0 37.0 37.0 5 36.63937756917107 37.0 37.0 37.0 37.0 37.0 6 36.54046937753188 37.0 37.0 37.0 37.0 37.0 7 36.48052712833332 37.0 37.0 37.0 37.0 37.0 8 36.4251995982553 37.0 37.0 37.0 37.0 37.0 9 36.44433312491998 37.0 37.0 37.0 37.0 37.0 10-11 36.39358084801446 37.0 37.0 37.0 37.0 37.0 12-13 36.370561013112635 37.0 37.0 37.0 37.0 37.0 14-15 38.20837197060004 40.0 37.0 40.0 37.0 40.0 16-17 38.21620052306365 40.0 37.0 40.0 37.0 40.0 18-19 38.19178337607257 40.0 37.0 40.0 37.0 40.0 20-21 38.147705350339535 40.0 37.0 40.0 37.0 40.0 22-23 37.99996876907262 40.0 37.0 40.0 33.0 40.0 24-25 38.00654823981871 40.0 37.0 40.0 33.0 40.0 26-27 37.9412773107468 40.0 37.0 40.0 33.0 40.0 28-29 37.770094227281604 40.0 37.0 40.0 33.0 40.0 30-31 37.72099112979511 40.0 37.0 40.0 33.0 40.0 32-33 37.5950771978804 40.0 37.0 40.0 33.0 40.0 34-35 37.545043698437894 40.0 37.0 40.0 33.0 40.0 36-37 37.47931456038663 40.0 37.0 40.0 33.0 40.0 38-39 37.28154183825549 40.0 37.0 40.0 33.0 40.0 40-41 37.15724818549866 40.0 37.0 40.0 33.0 40.0 42-43 37.00940548122975 40.0 37.0 40.0 33.0 40.0 44-45 36.852854941820354 37.0 37.0 40.0 33.0 40.0 46-47 36.87834139847432 37.0 37.0 40.0 33.0 40.0 48-49 36.60061781300226 37.0 37.0 40.0 33.0 40.0 50-51 36.52732442003702 37.0 37.0 40.0 33.0 40.0 52-53 36.466911687017 37.0 37.0 40.0 33.0 40.0 54-55 36.107340852787665 37.0 37.0 40.0 30.0 40.0 56-57 35.97939893050388 37.0 37.0 40.0 27.0 40.0 58-59 35.83845266758733 37.0 37.0 40.0 27.0 40.0 60-61 35.57762175089591 37.0 35.0 40.0 27.0 40.0 62-63 35.32788744684527 37.0 33.0 40.0 27.0 40.0 64-65 35.14713884306971 37.0 33.0 40.0 27.0 40.0 66-67 34.68921669105062 37.0 33.0 40.0 27.0 40.0 68-69 34.52387099420628 37.0 33.0 37.0 27.0 40.0 70-71 34.15089541088713 37.0 33.0 37.0 27.0 40.0 72-73 33.71270234844144 37.0 33.0 37.0 27.0 40.0 74-75 33.111472968622394 37.0 33.0 37.0 22.0 40.0 76-77 30.602010153003576 33.0 30.0 35.0 22.0 37.0 78-79 32.64490233576261 37.0 33.0 37.0 22.0 37.0 80-81 32.888351920655424 37.0 33.0 37.0 22.0 37.0 82-83 32.78072626045537 37.0 33.0 37.0 22.0 37.0 84-85 32.590338021183584 37.0 33.0 37.0 22.0 37.0 86-87 32.413182372456625 37.0 33.0 37.0 22.0 37.0 88-89 32.16101827118016 37.0 33.0 37.0 22.0 37.0 90-91 31.879292154915344 33.0 33.0 37.0 18.5 37.0 92-93 31.85061097638134 33.0 33.0 37.0 22.0 37.0 94-95 31.761183158046517 33.0 33.0 37.0 18.5 37.0 96-97 31.58493320621362 33.0 33.0 37.0 15.0 37.0 98-99 31.41870605140393 33.0 33.0 37.0 15.0 37.0 100-101 29.833436141610036 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.17996299976789487 2306 2 0.15255106553243536 2306 3 0.09223657943940111 2306 4 0.06497145354003919 2306 5 0.13041609465042825 2306 6 0.1188264445407583 2306 7 0.11367588929309846 2306 8 0.059870194034793656 2306 9 0.11112536005073537 2306 10-11 0.15496442526303156 2306 12-13 0.17057282708446309 2306 14-15 0.3675305573903316 2306 16-17 0.3318996838314021 2306 18-19 0.41565369103971506 2306 20-21 0.42439400792277837 2306 22-23 0.4018010517529973 2306 24-25 0.4204293818862155 2306 26-27 0.3111340181732274 2306 28-29 0.4944828743677405 2306 30-31 0.4016268253079289 2306 32-33 0.5124099934725521 2306 34-35 0.4341692685266878 2306 36-37 0.4372762004843338 2306 38-39 0.5073384690115788 2306 40-41 0.403045776355448 2306 42-43 0.4915059375534341 2306 44-45 0.5283448013571146 2306 46-47 0.38617509936160843 2306 48-49 0.42036416166940427 2306 50-51 0.38340314992651514 2306 52-53 0.21822353257138616 2306 54-55 0.20910421166694704 2306 56-57 0.25231237877487445 2306 58-59 0.4028305863365773 2306 60-61 0.29432942665423667 2306 62-63 0.3350918552571116 2306 64-65 0.20176695361883645 2306 66-67 0.197342652354088 2306 68-69 0.3435967062659415 2306 70-71 0.42528169051758624 2306 72-73 0.41583134532508126 2306 74-75 0.5227995821667193 2306 76-77 0.1851356409818976 2306 78-79 0.4429084993107395 2306 80-81 0.5205709286433304 2306 82-83 0.486526355289314 2306 84-85 0.5668366900517441 2306 86-87 0.4430389469722442 2306 88-89 0.5439948785280251 2306 90-91 0.484832852800654 2306 92-93 0.4229853247121298 2306 94-95 0.4602376195367128 2306 96-97 0.49541111926654935 2306 98-99 0.3822492694933928 2306 100-101 0.40050876386125367 2307 1 0.15983215857401234 2307 2 0.17093637913122706 2307 3 0.12915191992428987 2307 4 0.12562160753739704 2307 5 0.15089587830610185 2307 6 0.16211487667012392 2307 7 0.1729009231427554 2307 8 0.1485637448973378 2307 9 0.23993873351517436 2307 10-11 0.16328870042779897 2307 12-13 0.2179641866413462 2307 14-15 0.355364450179799 2307 16-17 0.27895062066649956 2307 18-19 0.32309497056606773 2307 20-21 0.2337912200111134 2307 22-23 0.2903190307483712 2307 24-25 0.3798861200528023 2307 26-27 0.37720796503247556 2307 28-29 0.4528336769048238 2307 30-31 0.3261724001080921 2307 32-33 0.3835122378941094 2307 34-35 0.4331677914320551 2307 36-37 0.38199358460618527 2307 38-39 0.2908750090147194 2307 40-41 0.3273755041538351 2307 42-43 0.31884209408674025 2307 44-45 0.4817921674020411 2307 46-47 0.3452333664342646 2307 48-49 0.3771525702573868 2307 50-51 0.27479215901349363 2307 52-53 0.2107325817378296 2307 54-55 0.2419536183322748 2307 56-57 0.35311180758541383 2307 58-59 0.3012936293145927 2307 60-61 0.22595892068971324 2307 62-63 0.2053426352311547 2307 64-65 0.35991523908231216 2307 66-67 0.1612326107398374 2307 68-69 0.3235017376615872 2307 70-71 0.4091884519503921 2307 72-73 0.3846893516284595 2307 74-75 0.35452024557319106 2307 76-77 0.03708946399102331 2307 78-79 0.45916357217387826 2307 80-81 0.717731572417712 2307 82-83 0.5065252220415957 2307 84-85 0.5158020206207112 2307 86-87 0.5572906987673534 2307 88-89 0.7481151104035533 2307 90-91 0.8024639452045683 2307 92-93 0.5179258016911916 2307 94-95 0.5832035570862075 2307 96-97 0.38271116270091454 2307 98-99 0.30740029615263964 2307 100-101 0.20325436218241322 2201 1 0.11514926203007292 2201 2 0.16549153123929017 2201 3 0.13336541681063352 2201 4 0.11389320086232146 2201 5 0.19513439729844606 2201 6 0.20688149379427045 2201 7 0.12445022421701424 2201 8 0.12930782331247315 2201 9 0.1786039076351642 2201 10-11 0.17438167730587395 2201 12-13 0.14790699325620693 2201 14-15 0.35189108084974663 2201 16-17 0.3445218960413641 2201 18-19 0.3291796694541844 2201 20-21 0.3503306439382712 2201 22-23 0.44709155534717837 2201 24-25 0.4649400210555612 2201 26-27 0.3806688233095272 2201 28-29 0.45017672433395006 2201 30-31 0.4535396230305935 2201 32-33 0.447529696927468 2201 34-35 0.3974274757396188 2201 36-37 0.37896054790082445 2201 38-39 0.3797338692860066 2201 40-41 0.4997428211444088 2201 42-43 0.5256869570295706 2201 44-45 0.48544652369425734 2201 46-47 0.5507088805162539 2201 48-49 0.5454692221867319 2201 50-51 0.4055770629699893 2201 52-53 0.36044219361298246 2201 54-55 0.4447250517414929 2201 56-57 0.48336974222691964 2201 58-59 0.4134888017413658 2201 60-61 0.39154091982428696 2201 62-63 0.39986514571973686 2201 64-65 0.5845226930359928 2201 66-67 0.4451509220298888 2201 68-69 0.45937657069077176 2201 70-71 0.5152761610166792 2201 72-73 0.2770916619402044 2201 74-75 0.35559986478565264 2201 76-77 0.22229603746758997 2201 78-79 0.5107822014140027 2201 80-81 0.5192850124869466 2201 82-83 0.3706983637269232 2201 84-85 0.43735339119245964 2201 86-87 0.5622626664831998 2201 88-89 0.6730426779914538 2201 90-91 0.6813743547769384 2201 92-93 0.7342043748667493 2201 94-95 0.34895835926106145 2201 96-97 0.48056819805169226 2201 98-99 0.33710877921097904 2201 100-101 0.45736278503719063 1101 1 0.13883217960742655 1101 2 0.11538712561078057 1101 3 0.11146335579295652 1101 4 0.09788554204113353 1101 5 0.1270689721796785 1101 6 0.09751280464487166 1101 7 0.1604340395382735 1101 8 0.16909883142287896 1101 9 0.1467910791096685 1101 10-11 0.13842450528947126 1101 12-13 0.10539963247877182 1101 14-15 0.19346619690617928 1101 16-17 0.26249718550506174 1101 18-19 0.2582084080969125 1101 20-21 0.29943669332320866 1101 22-23 0.16419547326773198 1101 24-25 0.15711158587556184 1101 26-27 0.2663133203381207 1101 28-29 0.32234892136796134 1101 30-31 0.3165722633369512 1101 32-33 0.15295156430703116 1101 34-35 0.1508836091187078 1101 36-37 0.13652372414402691 1101 38-39 0.23650569421692325 1101 40-41 0.26640924696764046 1101 42-43 0.3527452652248755 1101 44-45 0.41934620036193593 1101 46-47 0.26552291928000216 1101 48-49 0.48517777152651576 1101 50-51 0.3532826679988048 1101 52-53 0.2783617579272146 1101 54-55 0.6234759774834373 1101 56-57 0.2469271201644645 1101 58-59 0.40650970093032157 1101 60-61 0.3678932255991114 1101 62-63 0.3452538670217393 1101 64-65 0.36528496250367226 1101 66-67 0.3401930997362186 1101 68-69 0.36245391755950607 1101 70-71 0.2766142871343362 1101 72-73 0.3619741604499822 1101 74-75 0.3112230471703654 1101 76-77 0.0927184467448825 1101 78-79 0.4103894158790524 1101 80-81 0.5099663585608951 1101 82-83 0.504337297272194 1101 84-85 0.4796369482091336 1101 86-87 0.4103023514599826 1101 88-89 0.43525840747611255 1101 90-91 0.56560106672217 1101 92-93 0.4025876230017147 1101 94-95 0.17571175966929786 1101 96-97 0.3201914888159507 1101 98-99 0.21078311674100902 1101 100-101 0.37165940178905643 2304 1 0.11646479500228679 2304 2 0.11499075626845467 2304 3 0.08276765064410796 2304 4 0.04977674751237515 2304 5 0.16887311228494184 2304 6 0.1559366685222372 2304 7 0.16007171887211058 2304 8 0.10330411623547064 2304 9 0.09970756198219277 2304 10-11 0.11650853284172058 2304 12-13 0.1490534707341169 2304 14-15 0.25675054747807025 2304 16-17 0.22002466835972 2304 18-19 0.15309159441783038 2304 20-21 0.14324373535286838 2304 22-23 0.21995750936024905 2304 24-25 0.18327295491938855 2304 26-27 0.23030043253248067 2304 28-29 0.18737332724527533 2304 30-31 0.18381861253253362 2304 32-33 0.21901843987682668 2304 34-35 0.23371296860509005 2304 36-37 0.3727106877151485 2304 38-39 0.3331754588550595 2304 40-41 0.26132759656265137 2304 42-43 0.2713259484489683 2304 44-45 0.32548071828383485 2304 46-47 0.25815275505087243 2304 48-49 0.3755287431429011 2304 50-51 0.22831310950019912 2304 52-53 0.2707986003049001 2304 54-55 0.2902660133444783 2304 56-57 0.4024675802979658 2304 58-59 0.251677926583568 2304 60-61 0.2154071454766182 2304 62-63 0.2563356728596986 2304 64-65 0.3919278989225816 2304 66-67 0.20007302547651307 2304 68-69 0.20798713586022188 2304 70-71 0.4453980791094452 2304 72-73 0.32387018006813406 2304 74-75 0.26116950829906926 2304 76-77 0.3552612995994835 2304 78-79 0.5143449101368134 2304 80-81 0.618222902968391 2304 82-83 0.36512424615505523 2304 84-85 0.500518787546099 2304 86-87 0.45963056191805407 2304 88-89 0.5842817525005088 2304 90-91 0.6214303604014724 2304 92-93 0.35506139674169646 2304 94-95 0.3235470654592305 2304 96-97 0.4086981215133392 2304 98-99 0.29854327420163074 2304 100-101 0.23301471926853168 2202 1 0.13370934022157854 2202 2 0.1357279974876775 2202 3 0.12160705378764902 2202 4 0.08619827385157208 2202 5 0.1492602604002542 2202 6 0.0810429844775058 2202 7 0.12092530067443619 2202 8 0.14539982444751587 2202 9 0.1093215038769273 2202 10-11 0.07674082224392009 2202 12-13 0.04841776035272005 2202 14-15 0.20909478864052033 2202 16-17 0.19063470620625367 2202 18-19 0.11228084547965267 2202 20-21 0.09063142900621557 2202 22-23 0.1080613822538794 2202 24-25 0.15246784525078283 2202 26-27 0.0422778973076916 2202 28-29 0.09869408095657661 2202 30-31 0.09930372430120826 2202 32-33 0.1380165021108084 2202 34-35 0.26949907771962955 2202 36-37 0.15822356142669491 2202 38-39 0.16462099606344793 2202 40-41 0.17404177754002603 2202 42-43 0.20381461727486538 2202 44-45 0.16894874647699254 2202 46-47 0.15595338661708524 2202 48-49 0.2259445193459797 2202 50-51 0.12868958937539077 2202 52-53 0.14181070261446393 2202 54-55 0.22500092903127467 2202 56-57 0.2448257437403072 2202 58-59 0.24796589604410002 2202 60-61 0.23001807718468825 2202 62-63 0.16728498366153843 2202 64-65 0.0536846566517184 2202 66-67 -0.027916833432477972 2202 68-69 -7.093453794766447E-4 2202 70-71 0.07759857486413324 2202 72-73 -0.034564232375146275 2202 74-75 -0.09676054130571998 2202 76-77 -0.29850873599774275 2202 78-79 0.14704303945038077 2202 80-81 0.17866862379948856 2202 82-83 0.263763439238474 2202 84-85 0.13453219120506787 2202 86-87 0.17397011044069188 2202 88-89 0.32217957618485116 2202 90-91 0.33884432356339644 2202 92-93 0.14056074526747864 2202 94-95 0.24783167640958936 2202 96-97 -0.009315933486597316 2202 98-99 -0.08067048742836036 2202 100-101 0.07312483693365124 1102 1 0.15521003809365652 1102 2 0.12409991521934671 1102 3 0.07001182478944656 1102 4 0.08118321177084908 1102 5 0.1333469576849211 1102 6 0.07259444377089608 1102 7 0.024029466853761505 1102 8 0.07786478139257724 1102 9 0.0412609124660861 1102 10-11 0.10481256401305927 1102 12-13 0.0869283427964902 1102 14-15 0.14772714131414233 1102 16-17 0.08327209083643794 1102 18-19 0.1882134858793023 1102 20-21 0.10816452269781252 1102 22-23 0.03385795709253614 1102 24-25 0.16801310816791926 1102 26-27 0.1274905468563503 1102 28-29 0.1807634471015902 1102 30-31 0.284091282684642 1102 32-33 0.18217516988990923 1102 34-35 0.1486811768460541 1102 36-37 0.2100319208165189 1102 38-39 6.727815503140278E-4 1102 40-41 0.06836367200929772 1102 42-43 0.23334240349988278 1102 44-45 0.07853363605205033 1102 46-47 0.1977464370068276 1102 48-49 0.2268444754203287 1102 50-51 0.21852859795519208 1102 52-53 0.10864462465552549 1102 54-55 0.30468453436576937 1102 56-57 0.298446881076849 1102 58-59 0.23409188729535657 1102 60-61 0.09928285530815373 1102 62-63 0.09511292505587932 1102 64-65 0.20802432931463244 1102 66-67 0.23891903797191816 1102 68-69 0.07914515414098133 1102 70-71 0.19686101375159382 1102 72-73 0.012756302738090142 1102 74-75 0.05862853862077344 1102 76-77 -0.13863819971489022 1102 78-79 0.19794676429384594 1102 80-81 0.19039612359805602 1102 82-83 0.18306317594446853 1102 84-85 0.15789570068287162 1102 86-87 0.01769744909189086 1102 88-89 0.12508802628504867 1102 90-91 0.19283220112258803 1102 92-93 0.06034665318994925 1102 94-95 -0.052184698242442096 1102 96-97 -0.15236853250775795 1102 98-99 -0.22359793732322686 1102 100-101 -0.006105346488443075 2305 1 0.11939156685953378 2305 2 0.11638678351245346 2305 3 0.09071264450595606 2305 4 0.11117270813789304 2305 5 0.07493809565067977 2305 6 0.12029393158343282 2305 7 0.053966986480396884 2305 8 0.11917004728609015 2305 9 0.07560129514483549 2305 10-11 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-1.4464547667163075 1213 94-95 -1.4343852513855353 1213 96-97 -1.4666903721824198 1213 98-99 -1.474849832829154 1213 100-101 -1.5126413955648133 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 15.0 10 17.0 11 16.0 12 47.0 13 123.0 14 351.0 15 846.0 16 1619.0 17 2566.0 18 4071.0 19 5611.0 20 7564.0 21 10170.0 22 13374.0 23 17160.0 24 21956.0 25 27513.0 26 35962.0 27 49366.0 28 70928.0 29 81413.0 30 68519.0 31 75911.0 32 100195.0 33 138194.0 34 204364.0 35 330042.0 36 582005.0 37 952148.0 38 414985.0 39 910.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.84396393894688 26.401904726919305 19.615038700661444 16.139092633472377 2 29.636938138524854 27.469045645010326 28.454233795033133 14.439782421431687 3 34.941907367515604 21.081652871194333 27.298907010768296 16.67753275052176 4 29.48844173691875 21.742376071340445 28.321738087290978 20.447444104449826 5 34.16980426132797 20.017874656149043 28.80277715971962 17.009543922803363 6 30.909295442708494 20.734166075195372 31.14264174490454 17.213896737191593 7 31.238136908927512 24.621064747772195 25.161996840238114 18.978801503062183 8 28.693297998361693 30.083400560105705 23.10321060148591 18.120090840046686 9 31.675463118916188 24.59163620226951 24.834926680348197 18.89797399846611 10-11 26.28540911641991 29.95766475257358 28.01648349257867 15.740442638427837 12-13 24.090123444513363 29.32565435784863 27.75383751962421 18.8303846780138 14-15 23.368626870903466 29.604013593688432 27.992388976383825 19.034970559024277 16-17 24.834020908649777 27.666475913784254 28.23279910613914 19.26670407142683 18-19 22.10467550289562 27.78489753148264 30.47762498275307 19.63280198286867 20-21 23.924242160571094 27.81959338264816 30.4435351048054 17.81262935197535 22-23 21.657917863593255 32.308301149422434 28.050267187575745 17.98351379940857 24-25 21.363647946341228 28.011376137209737 30.39771420687118 20.227261709577856 26-27 21.335462422823873 27.959184751600702 28.279589206094286 22.42576361948114 28-29 23.24061114418931 30.298598741315935 28.1210728274151 18.339717287079658 30-31 20.942698551009272 28.20729193987254 30.358077343313973 20.491932165804215 32-33 20.888238650277007 30.402142472693917 28.18883306339039 20.520785813638685 34-35 20.772581909736065 28.294623027051585 30.40774552100195 20.5250495422104 36-37 22.93024409222726 28.17829845206472 28.27920076172387 20.612256693984147 38-39 22.84755714507466 30.403250955713297 28.19600889963577 18.553182999576272 40-41 22.699411574949142 30.28176280136816 28.24211823073447 18.77670739294822 42-43 20.551829133754026 30.248749014096937 30.295565813574356 18.90385603857468 44-45 20.37458375654946 28.190381453103925 30.455370451426912 20.979664338919708 46-47 22.52923620165804 28.165472729168485 28.153802955482842 21.151488113690633 48-49 20.301252756852914 30.462352573536666 30.165693211860095 19.07070145775032 50-51 22.437010045761436 28.18497253536994 30.177032266084485 19.200985152784146 52-53 20.383681195727352 28.262108288670547 27.998661165261712 23.35554935034039 54-55 22.29568587203048 28.23373106688718 30.29801576657291 19.172567294509424 56-57 20.199146625471776 28.22639990488705 30.248948823171506 21.32550464646967 58-59 20.103508177780167 28.495149501537202 30.203769582633477 21.197572738049146 60-61 22.104643605045677 28.328822529421764 30.28367864011307 19.28285522541949 62-63 20.034485362834438 28.435195582293243 30.138827205520148 21.39149184935217 64-65 22.05013875977449 28.40060905735041 30.197897017838145 19.351355165036953 66-67 20.135628286364856 32.29133754126873 27.95994498901288 19.613089183353534 68-69 20.0459300350288 32.511088516341125 27.919126334639593 19.523855113990475 70-71 19.98630390564056 32.40131918940632 28.008746103288633 19.603630801664483 72-73 19.879009885910286 32.392915589774354 27.941489584903668 19.786584939411693 74-75 19.844271492211167 32.05580686009319 28.16705500872662 19.932866638969028 76-77 19.947158506916193 31.753948074583626 28.14041867534437 20.158474743155807 78-79 19.928451583986632 31.59961793599651 28.18359213517581 20.288338344841048 80-81 19.901927831676137 31.327198694815948 28.29046094633924 20.480412527168674 82-83 19.976335479921286 31.242946675585532 28.132173259092312 20.64854458540087 84-85 19.944068675226 31.04479105546329 28.350798210893757 20.660342058416955 86-87 20.097698125846563 30.78860229977596 28.34147095827625 20.77222861610123 88-89 20.146404237330284 30.909415875068387 28.201217237777886 20.742962649823447 90-91 20.184312918564512 30.543514546292077 28.247047319353257 21.02512521579015 92-93 20.140167448570033 30.715185091916393 28.148614492104794 20.996032967408777 94-95 20.061315780310306 30.625369971496653 28.278522626088776 21.034791622104272 96-97 20.150779059855893 30.527094098344143 28.224063098221663 21.098063743578297 98-99 20.13517702093237 30.59837772652278 28.140671647707187 21.125773604837658 100-101 20.27202490346503 30.49163544950919 27.984298854015933 21.252040793009844 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7.0 1 5.0 2 3.0 3 10.5 4 15.0 5 10.0 6 7.5 7 10.0 8 15.5 9 22.5 10 30.5 11 30.0 12 33.5 13 48.0 14 90.0 15 135.0 16 204.0 17 362.5 18 634.0 19 1086.5 20 1829.0 21 2950.5 22 4585.0 23 7032.0 24 10247.0 25 14241.0 26 19298.0 27 25391.5 28 32097.0 29 39798.0 30 48346.5 31 57571.5 32 67813.5 33 78561.5 34 90667.5 35 103753.5 36 117539.5 37 131920.0 38 144637.0 39 154145.5 40 160555.0 41 162880.0 42 162361.5 43 161351.5 44 156926.5 45 149112.0 46 140581.5 47 130985.5 48 119412.0 49 107211.5 50 96225.5 51 86405.0 52 76403.0 53 65409.0 54 55242.5 55 46472.5 56 38423.5 57 31579.0 58 25763.5 59 20833.0 60 16757.5 61 13093.5 62 9957.0 63 7522.5 64 5585.0 65 4184.0 66 3123.0 67 2269.0 68 1649.5 69 1161.0 70 872.5 71 648.5 72 467.0 73 343.5 74 261.5 75 189.5 76 132.5 77 98.0 78 69.0 79 61.0 80 52.0 81 41.0 82 32.5 83 19.5 84 14.0 85 15.5 86 10.5 87 7.5 88 6.0 89 3.0 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17852903264300035 2 0.0 3 0.0 4 0.008266096202443533 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0024238928713932162 12-13 0.0 14-15 0.0 16-17 4.195199200488259E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 3.107554963324636E-5 36-37 0.0 38-39 0.002470506195843086 40-41 0.006898772018580693 42-43 0.0031697060625911293 44-45 0.0014139375083127095 46-47 0.007753349633494968 48-49 0.009338202664790532 50-51 0.0058732788806835625 52-53 0.00747366968679575 54-55 0.02023018281124338 56-57 0.022887142304885946 58-59 0.028698270086303018 60-61 0.027128954829824078 62-63 0.011404726715401416 64-65 0.016563267954520313 66-67 0.024503070885814757 68-69 0.027874768021021987 70-71 0.03071818081246403 72-73 0.02358634217163399 74-75 0.033670358027622437 76-77 0.030593878613931046 78-79 0.02181503584253895 80-81 0.010363695802687662 82-83 0.0020665240506108832 84-85 0.008328247301710025 86-87 0.02832536349070406 88-89 0.02622776389045993 90-91 0.022312244636670888 92-93 0.04518384916674022 94-95 0.04661332444986955 96-97 0.03536397548263436 98-99 0.042480276348647775 100-101 0.042993022917596345 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3217964.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.01390934161287 #Duplication Level Percentage of deduplicated Percentage of total 1 97.67667075968494 87.9225898655285 2 1.683026798467588 3.029916433135328 3 0.24520032562441707 0.6621431964387071 4 0.11048223157204982 0.39779750306342293 5 0.06436330958878982 0.2896796557125744 6 0.03132797447142723 0.1691972072356448 7 0.0326486007187486 0.20571797296595676 8 0.016826028659691447 0.1211661294682277 9 0.01048298155279142 0.08492527360104903 >10 0.11178330982324944 2.004786354925031 >50 0.013344012257301727 0.8272686804174987 >100 0.0035794230210812947 0.512915095762199 >500 1.5909264787957396E-4 0.11219400666902866 >1k 7.08062034637364E-5 0.12861068357741717 >5k 0.0 0.0 >10k+ 3.434570659278849E-5 3.531091941499421 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 114216 3.5493249769108663 TruSeq Adapter, Index 6 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0018023818787282892 0.0 0.0 2 0.0019888351765277672 0.0 0.0 3 0.002299590672860231 0.0 0.0 4 0.0025792706195594483 0.0 0.0 5 0.0034183104596571 0.0 0.0 6 0.003915519253789042 0.0 0.0 7 0.004599181345720462 0.0 3.107554963324636E-5 8 0.005407145636184867 0.0 6.215109926649273E-5 9 0.0061529588273827795 0.0 6.215109926649273E-5 10-11 0.008654540572859113 0.0 6.215109926649273E-5 12-13 0.011684406662100633 0.0 9.322664889973908E-5 14-15 0.014885188274325008 0.0 1.5537774816623182E-4 16-17 0.018039356562099512 0.0 1.864532977994782E-4 18-19 0.021426591472123366 0.0 1.864532977994782E-4 20-21 0.02523334630219605 0.0 1.864532977994782E-4 22-23 0.029568385476033916 0.0 1.864532977994782E-4 24-25 0.03495999333740216 0.0 2.0199107261610136E-4 26-27 0.04114402771441819 0.0 2.1752884743272453E-4 28-29 0.04740575096551733 0.0 2.486043970659709E-4 30-31 0.08020599360340885 0.0 2.7967994669921725E-4 32-33 0.08928005409631681 0.0 2.7967994669921725E-4 34-35 0.09740631032541072 0.0 2.952177215158404E-4 36-37 0.10694650406281736 0.0 3.262932711490868E-4 38-39 0.11821139080486917 0.0 4.039821452322027E-4 40-41 0.1308436017307838 0.0 4.3505769486544907E-4 42-43 0.14501405236354414 0.0 4.3505769486544907E-4 44-45 0.15916896522148788 0.0 4.3505769486544907E-4 46-47 0.17545255322930897 0.0 4.3505769486544907E-4 48-49 0.19282378547429369 0.0 4.3505769486544907E-4 50-51 0.21097190646010955 0.0 4.5059546968207224E-4 52-53 0.23072041825203762 0.0 4.6613324449869546E-4 54-55 0.2509661388380976 0.0 4.972087941319418E-4 56-57 0.27458355655936484 0.0 4.972087941319418E-4 58-59 0.2984029653532482 0.0 4.972087941319418E-4 60-61 0.32402475602585984 0.0 5.12746568948565E-4 62-63 0.35253657281436335 0.0 5.282843437651881E-4 64-65 0.3838607268446757 0.0 5.282843437651881E-4 66-67 0.41889840905616094 0.0 5.282843437651881E-4 68-69 0.4553344910011423 0.0 5.438221185818113E-4 70-71 0.49708449193340887 0.0 5.748976682150577E-4 72-73 0.5421285011267994 0.0 6.059732178483042E-4 74-75 0.5923621271089421 0.0 6.370487674815504E-4 76-77 0.647210472211622 0.0 6.525865422981736E-4 78-79 0.7065181586866727 0.0 6.525865422981736E-4 80-81 0.7724760127832382 0.0 6.8366209193142E-4 82-83 0.8460318387651322 0.0 6.8366209193142E-4 84-85 0.9260047657462918 0.0 7.302754163812895E-4 86-87 1.0157509530871072 0.0 7.61350966014536E-4 88-89 1.1167309516203412 0.0 8.079642904644054E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 18490 0.0 70.40384 3 CGGAAGA 18525 0.0 69.80936 4 GATCGGA 18665 0.0 69.676605 1 ATCGGAA 18740 0.0 69.21119 2 GAAGAGC 19650 0.0 65.883095 6 AGAGCAC 19995 0.0 64.48507 8 GAGCACA 20060 0.0 64.27611 9 AAGAGCA 21355 0.0 60.356075 7 GGAAGAG 23245 0.0 56.061573 5 CGACGGG 55 8.0942664E-7 51.911533 1 ATGCCGT 14220 0.0 44.440952 48-49 GCCGTCT 14275 0.0 44.437473 50-51 CGTCTTC 14345 0.0 44.208195 52-53 TCGTATG 14495 0.0 44.023457 44-45 TGCCGTC 14235 0.0 43.961723 50-51 TCTCGTA 14225 0.0 43.75719 42-43 TATGCCG 14320 0.0 43.749172 48-49 CGTATGC 14385 0.0 43.617188 46-47 CTCGTAT 14125 0.0 43.562588 44-45 CCGTCTT 14400 0.0 43.561016 52-53 >>END_MODULE