FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_52150223-1.3410000003573e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_52150223-1.3410000003573e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3217964
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT1142163.5493249769108663TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG184900.070.403843
CGGAAGA185250.069.809364
GATCGGA186650.069.6766051
ATCGGAA187400.069.211192
GAAGAGC196500.065.8830956
AGAGCAC199950.064.485078
GAGCACA200600.064.276119
AAGAGCA213550.060.3560757
GGAAGAG232450.056.0615735
CGACGGG558.0942664E-751.9115331
ATGCCGT142200.044.44095248-49
GCCGTCT142750.044.43747350-51
CGTCTTC143450.044.20819552-53
TCGTATG144950.044.02345744-45
TGCCGTC142350.043.96172350-51
TCTCGTA142250.043.7571942-43
TATGCCG143200.043.74917248-49
CGTATGC143850.043.61718846-47
CTCGTAT141250.043.56258844-45
CCGTCTT144000.043.56101652-53
Produced by FastQC (version 0.11.2)