##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_50331580-1.341000000357a8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3126411 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66430517292832 33.0 33.0 33.0 33.0 33.0 2 32.67993395622009 33.0 33.0 33.0 33.0 33.0 3 32.73699779075752 33.0 33.0 33.0 33.0 33.0 4 36.681840935180944 37.0 37.0 37.0 37.0 37.0 5 36.639104071729534 37.0 37.0 37.0 37.0 37.0 6 36.545253327217694 37.0 37.0 37.0 37.0 37.0 7 36.499528372949044 37.0 37.0 37.0 37.0 37.0 8 36.44275848568854 37.0 37.0 37.0 37.0 37.0 9 36.459739298511934 37.0 37.0 37.0 37.0 37.0 10-11 36.40976490295102 37.0 37.0 37.0 37.0 37.0 12-13 36.383068476921295 37.0 37.0 37.0 37.0 37.0 14-15 38.22888209515639 40.0 37.0 40.0 37.0 40.0 16-17 38.215211307790305 40.0 37.0 40.0 37.0 40.0 18-19 38.20567177508012 40.0 37.0 40.0 37.0 40.0 20-21 38.16211272286337 40.0 37.0 40.0 37.0 40.0 22-23 38.06302418332075 40.0 37.0 40.0 35.0 40.0 24-25 38.02706106138956 40.0 37.0 40.0 35.0 40.0 26-27 37.93991432988177 40.0 37.0 40.0 33.0 40.0 28-29 37.80614145101204 40.0 37.0 40.0 33.0 40.0 30-31 37.763097846060546 40.0 37.0 40.0 33.0 40.0 32-33 37.64567726380184 40.0 37.0 40.0 33.0 40.0 34-35 37.569389309339044 40.0 37.0 40.0 33.0 40.0 36-37 37.511801871219106 40.0 37.0 40.0 33.0 40.0 38-39 37.38960728451889 40.0 37.0 40.0 33.0 40.0 40-41 37.29745161464695 40.0 37.0 40.0 33.0 40.0 42-43 37.15707819605292 40.0 37.0 40.0 33.0 40.0 44-45 37.05741759480759 40.0 37.0 40.0 33.0 40.0 46-47 37.00649850579467 40.0 37.0 40.0 33.0 40.0 48-49 36.77918210369654 37.0 37.0 40.0 33.0 40.0 50-51 36.66756146264838 37.0 37.0 40.0 33.0 40.0 52-53 36.5659108799195 37.0 37.0 40.0 33.0 40.0 54-55 36.28077178592322 37.0 37.0 40.0 33.0 40.0 56-57 36.16164845249073 37.0 37.0 40.0 33.0 40.0 58-59 35.948162925475884 37.0 37.0 40.0 30.0 40.0 60-61 35.71766092174062 37.0 37.0 40.0 27.0 40.0 62-63 35.44895264889997 37.0 33.0 40.0 27.0 40.0 64-65 35.23490865404453 37.0 33.0 40.0 27.0 40.0 66-67 34.87145580027706 37.0 33.0 38.5 27.0 40.0 68-69 34.60419407429157 37.0 33.0 37.0 27.0 40.0 70-71 34.18329355929211 37.0 33.0 37.0 27.0 40.0 72-73 33.81918500158808 37.0 33.0 37.0 27.0 40.0 74-75 33.33497691122504 37.0 33.0 37.0 24.5 38.5 76-77 30.80179029564571 33.0 30.0 35.0 22.0 37.0 78-79 32.94522569169568 35.0 33.0 37.0 27.0 37.0 80-81 33.24383038570424 37.0 33.0 37.0 27.0 37.0 82-83 33.17032053687119 37.0 33.0 37.0 27.0 37.0 84-85 32.998677717037204 37.0 33.0 37.0 27.0 37.0 86-87 32.830552029147796 37.0 33.0 37.0 27.0 37.0 88-89 32.580887157830496 37.0 33.0 37.0 22.0 37.0 90-91 32.302618081883665 33.0 33.0 37.0 22.0 37.0 92-93 32.27229065532331 33.0 33.0 37.0 22.0 37.0 94-95 32.18110830597769 33.0 33.0 37.0 22.0 37.0 96-97 32.011030059707444 33.0 33.0 37.0 22.0 37.0 98-99 31.845466415004296 33.0 33.0 37.0 22.0 37.0 100-101 30.22441963004864 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.14502975009734342 2306 2 0.13783829292566452 2306 3 0.11015917224969485 2306 4 0.14069272592098514 2306 5 0.20644057317913678 2306 6 0.1957411532464377 2306 7 0.18262212087348928 2306 8 0.20488298366463198 2306 9 0.21131079542023912 2306 10-11 0.18392194587551813 2306 12-13 0.21705762455644617 2306 14-15 0.3650995369276515 2306 16-17 0.35261287688700094 2306 18-19 0.38586520420009407 2306 20-21 0.3563633141730449 2306 22-23 0.40227382402533607 2306 24-25 0.39458890467341234 2306 26-27 0.39010085807895933 2306 28-29 0.35217583038827627 2306 30-31 0.3493621510595304 2306 32-33 0.38127644253134463 2306 34-35 0.3651662982563124 2306 36-37 0.3280592339271493 2306 38-39 0.24791376790755493 2306 40-41 0.36890657146894057 2306 42-43 0.27860196211918975 2306 44-45 0.4200791723453534 2306 46-47 0.5227003683724973 2306 48-49 0.525440351764324 2306 50-51 0.30235234064514316 2306 52-53 0.30315301379734905 2306 54-55 0.37924813746204933 2306 56-57 0.4410069992593222 2306 58-59 0.5629135302949422 2306 60-61 0.5111943624652611 2306 62-63 0.37288861209604107 2306 64-65 0.38389846669670646 2306 66-67 0.3919073251239311 2306 68-69 0.327075219801813 2306 70-71 0.41878945459525596 2306 72-73 0.4529904443570345 2306 74-75 0.2844838919791144 2306 76-77 0.07071585712138173 2306 78-79 0.5166056797578591 2306 80-81 0.524225464387996 2306 82-83 0.5528680200338556 2306 84-85 0.5404229511863363 2306 86-87 0.48172596659044586 2306 88-89 0.5642781707517983 2306 90-91 0.5288814769227201 2306 92-93 0.5266239658916163 2306 94-95 0.6269967356527246 2306 96-97 0.5720803705761668 2306 98-99 0.5596438475978154 2306 100-101 0.4500880538853913 2307 1 0.13621415047023788 2307 2 0.14844969866373958 2307 3 0.08712895009968236 2307 4 0.1423666841213489 2307 5 0.15084053346574677 2307 6 0.1468553202257823 2307 7 0.11224173619512356 2307 8 0.14104467132911225 2307 9 0.1279997041658234 2307 10-11 0.172662296359114 2307 12-13 0.18115294572562135 2307 14-15 0.3662951292567769 2307 16-17 0.30917538615453566 2307 18-19 0.3211233477021125 2307 20-21 0.26996718210281045 2307 22-23 0.31537579460905363 2307 24-25 0.31159049191973764 2307 26-27 0.30580579659412166 2307 28-29 0.344100666344886 2307 30-31 0.29663649711017825 2307 32-33 0.2939483995893042 2307 34-35 0.349462745841727 2307 36-37 0.32559181373753177 2307 38-39 0.30949692685009467 2307 40-41 0.2865781610490785 2307 42-43 0.2765690683409048 2307 44-45 0.2531204575120185 2307 46-47 0.34841530615270244 2307 48-49 0.5038468389728763 2307 50-51 0.36165907116269125 2307 52-53 0.22310892935223592 2307 54-55 0.4963973283016614 2307 56-57 0.34864725007260944 2307 58-59 0.3257807341239314 2307 60-61 0.36741028212042437 2307 62-63 0.30255158335412347 2307 64-65 0.264165165340394 2307 66-67 0.1254473808516252 2307 68-69 0.20400077137313843 2307 70-71 0.26695100171558295 2307 72-73 0.260743452839975 2307 74-75 0.2100190235039463 2307 76-77 -0.10699496877536063 2307 78-79 0.1916716323570924 2307 80-81 0.4477818567614804 2307 82-83 0.29995672531277506 2307 84-85 0.297325570851946 2307 86-87 0.38215446507604867 2307 88-89 0.5395689939923898 2307 90-91 0.48900223529381037 2307 92-93 0.36723674483629765 2307 94-95 0.36707451201216657 2307 96-97 0.2550504810902865 2307 98-99 0.2739817363667889 2307 100-101 0.18895669925897352 2201 1 0.1728836007510708 2201 2 0.15483638893025642 2201 3 0.11460151770376825 2201 4 0.05952048326776804 2201 5 0.18909067893596188 2201 6 0.19154876152763478 2201 7 0.1579571209790558 2201 8 0.10221751429187975 2201 9 0.13203695914894098 2201 10-11 0.19329350318975713 2201 12-13 0.1565669576115667 2201 14-15 0.2713490323626573 2201 16-17 0.3105366586963285 2201 18-19 0.2539431934915086 2201 20-21 0.3177690601902441 2201 22-23 0.2999325182546002 2201 24-25 0.3026799847095347 2201 26-27 0.25603033200241754 2201 28-29 0.3548228559698785 2201 30-31 0.3115441509129795 2201 32-33 0.3202830736021198 2201 34-35 0.37089769083085855 2201 36-37 0.33952652134848194 2201 38-39 0.25638782053632525 2201 40-41 0.3111026743020773 2201 42-43 0.3837416629695909 2201 44-45 0.327989229986855 2201 46-47 0.42578476641559604 2201 48-49 0.4838930729376827 2201 50-51 0.4435429774092725 2201 52-53 0.3745689877350955 2201 54-55 0.3501601984594558 2201 56-57 0.4101872379480582 2201 58-59 0.4047786695424662 2201 60-61 0.340788015317095 2201 62-63 0.27396577296936897 2201 64-65 0.18365074857248942 2201 66-67 0.265978304532716 2201 68-69 0.35483421223675293 2201 70-71 0.3259431304987217 2201 72-73 1.4786490901030902E-4 2201 74-75 0.07874402271268366 2201 76-77 -0.008231952538693577 2201 78-79 0.3230683655464546 2201 80-81 0.27185571667670416 2201 82-83 0.06863047518191223 2201 84-85 0.14110121073829873 2201 86-87 0.14765860762955896 2201 88-89 0.3440670669685204 2201 90-91 0.35257917138673633 2201 92-93 0.3027903959561087 2201 94-95 0.09447154466323582 2201 96-97 0.30030784772635144 2201 98-99 0.2011754150468228 2201 100-101 0.3026535417640339 1101 1 0.1560569677603354 1101 2 0.16234067065681757 1101 3 0.11217369517772369 1101 4 0.05557373134615773 1101 5 0.10227587557810836 1101 6 0.12836985362710607 1101 7 0.13997868513073541 1101 8 0.13705261045580386 1101 9 0.09512332056802109 1101 10-11 0.1108066004856525 1101 12-13 0.09181402300711738 1101 14-15 0.2757500892111864 1101 16-17 0.21723050009200762 1101 18-19 0.1879254994654218 1101 20-21 0.24154405864639017 1101 22-23 0.15962417728696465 1101 24-25 0.18299963691379162 1101 26-27 0.1293389579463664 1101 28-29 0.14286280250990302 1101 30-31 0.18305149146080169 1101 32-33 0.25253420515069536 1101 34-35 0.22382406609649763 1101 36-37 0.14847504658191468 1101 38-39 0.20364107099424444 1101 40-41 0.2308505410872712 1101 42-43 0.17550393550551746 1101 44-45 0.16229044881297483 1101 46-47 0.21846960018035588 1101 48-49 0.3023989982353541 1101 50-51 0.22005214792424965 1101 52-53 0.19590442184806278 1101 54-55 0.3207104038633517 1101 56-57 0.1266016750858583 1101 58-59 0.10062800092458701 1101 60-61 0.03765905475543718 1101 62-63 0.18276351004218583 1101 64-65 0.2381455275005493 1101 66-67 0.018137934890681606 1101 68-69 0.11254833114156071 1101 70-71 0.23184861297834658 1101 72-73 0.15281032548399054 1101 74-75 0.17919491415597832 1101 76-77 -0.028522350763338977 1101 78-79 0.21937983445486253 1101 80-81 0.3363178678002612 1101 82-83 0.3790242778819888 1101 84-85 0.26629773391400846 1101 86-87 0.1891644439783775 1101 88-89 0.27582175618854166 1101 90-91 0.3497163067459468 1101 92-93 0.26800890989814263 1101 94-95 0.2225754069137338 1101 96-97 0.16202571381468545 1101 98-99 0.11639907046003728 1101 100-101 0.22809739378257987 2304 1 0.1581489194638266 2304 2 0.18524451487031968 2304 3 0.13648338474823163 2304 4 0.11027389614621086 2304 5 0.2089317797922874 2304 6 0.1217240392121468 2304 7 0.15808651145166408 2304 8 0.16034659357220704 2304 9 0.18897190631449945 2304 10-11 0.12544689914371787 2304 12-13 0.1536140431867139 2304 14-15 0.2564382135175123 2304 16-17 0.24432497424529487 2304 18-19 0.2097127392326783 2304 20-21 0.2024393601632184 2304 22-23 0.23696248825457644 2304 24-25 0.25055084264479177 2304 26-27 0.2062165014165913 2304 28-29 0.24082466306888506 2304 30-31 0.22166178978253015 2304 32-33 0.25593110421906573 2304 34-35 0.3189742359414538 2304 36-37 0.2836312703063939 2304 38-39 0.33269632946126393 2304 40-41 0.45093596805394043 2304 42-43 0.3779288505830465 2304 44-45 0.4361141463880571 2304 46-47 0.37866534228508897 2304 48-49 0.3101916864941998 2304 50-51 0.2828880541563805 2304 52-53 0.3842473404906457 2304 54-55 0.5465840123118753 2304 56-57 0.5041417820377276 2304 58-59 0.4572183214438752 2304 60-61 0.35466036642933574 2304 62-63 0.39352932580244726 2304 64-65 0.3891326461902338 2304 66-67 0.4821812537619721 2304 68-69 0.3061614882397308 2304 70-71 0.4506492721785591 2304 72-73 0.14714322159268534 2304 74-75 0.20332971387345822 2304 76-77 0.2574158324001452 2304 78-79 0.5002024894173545 2304 80-81 0.5949093318301308 2304 82-83 0.4093306714514213 2304 84-85 0.5333242700859415 2304 86-87 0.4098619854306307 2304 88-89 0.47410312705592617 2304 90-91 0.7453844196171673 2304 92-93 0.5258133695893861 2304 94-95 0.42849475578535134 2304 96-97 0.4280668129301475 2304 98-99 0.31509075982875245 2304 100-101 0.41978003596466706 2202 1 0.15096227387991945 2202 2 0.15677090802379468 2202 3 0.12290238524278863 2202 4 0.09447382974676799 2202 5 0.1427609206447613 2202 6 0.08855945549976951 2202 7 0.05701943113271568 2202 8 0.15723219708500125 2202 9 0.1237967887243343 2202 10-11 0.12573694341626407 2202 12-13 0.11067792482814554 2202 14-15 0.22087380887104757 2202 16-17 0.2733167718507943 2202 18-19 0.16264284912939075 2202 20-21 0.09599167410878806 2202 22-23 0.08528227597642513 2202 24-25 0.18257738241474897 2202 26-27 0.15147753813572962 2202 28-29 0.23737689385884408 2202 30-31 0.2579329546050815 2202 32-33 0.2914926328067864 2202 34-35 0.36671731136109287 2202 36-37 0.3072764121742537 2202 38-39 0.3035303427324081 2202 40-41 0.2760918535642958 2202 42-43 0.39170836066519144 2202 44-45 0.24683309230002237 2202 46-47 0.3043000290541542 2202 48-49 0.33388109465757765 2202 50-51 0.31472033491473184 2202 52-53 0.2995717943052085 2202 54-55 0.42358510834035457 2202 56-57 0.4314826593982062 2202 58-59 0.5337058534165848 2202 60-61 0.4451972578224783 2202 62-63 0.4112774843433087 2202 64-65 0.31274196236974916 2202 66-67 0.321622585602249 2202 68-69 0.3322152042639459 2202 70-71 0.24969204479923235 2202 72-73 0.20067917951720204 2202 74-75 0.345401889192992 2202 76-77 0.10095879915478179 2202 78-79 0.4971520539758103 2202 80-81 0.6342720097159287 2202 82-83 0.5126010628642632 2202 84-85 0.45723313403648547 2202 86-87 0.6056759444442932 2202 88-89 0.5517226382384877 2202 90-91 0.6000710511333907 2202 92-93 0.5231548284139222 2202 94-95 0.5825122659812934 2202 96-97 0.35302268731140174 2202 98-99 0.3376728853737241 2202 100-101 0.4070856128301479 1102 1 0.14528073025406485 1102 2 0.14011128929521277 1102 3 0.08609676240660491 1102 4 0.055783053762773704 1102 5 0.0917445871384146 1102 6 0.11024057985544289 1102 7 0.13049857304245194 1102 8 0.1418605599912297 1102 9 0.11128311255910006 1102 10-11 0.09338161867299988 1102 12-13 0.10746920977824459 1102 14-15 0.23393891907731756 1102 16-17 0.1775511602272033 1102 18-19 0.22897342250779928 1102 20-21 0.24892365641770198 1102 22-23 0.21232714966528476 1102 24-25 0.23562076942540244 1102 26-27 0.25505602351741175 1102 28-29 0.22407894926837457 1102 30-31 0.24063493452675289 1102 32-33 0.15659347521769007 1102 34-35 0.22000123780706815 1102 36-37 0.25141064450233586 1102 38-39 0.24503055344932534 1102 40-41 0.29703883439830747 1102 42-43 0.28030948880007145 1102 44-45 0.21730471595503786 1102 46-47 0.35042792232489006 1102 48-49 0.3766409429990958 1102 50-51 0.38044357957547703 1102 52-53 0.2701256372265064 1102 54-55 0.2988287309981601 1102 56-57 0.4118057498617631 1102 58-59 0.343653735537238 1102 60-61 0.30542426473586914 1102 62-63 0.35833398652896875 1102 64-65 0.2670064737697402 1102 66-67 0.24047637690811996 1102 68-69 0.15987713114216717 1102 70-71 0.34339159684846976 1102 72-73 0.29539324999834093 1102 74-75 0.2538065012611028 1102 76-77 0.022083657284476033 1102 78-79 0.4534295550582428 1102 80-81 0.34271678180869003 1102 82-83 0.25626200551536016 1102 84-85 0.264075315597303 1102 86-87 0.1423024038062195 1102 88-89 0.2858609911461727 1102 90-91 0.5003417256126568 1102 92-93 0.39987106544485584 1102 94-95 0.24423545919488987 1102 96-97 0.1022000739359612 1102 98-99 0.030356019774092857 1102 100-101 0.22989660299773362 2305 1 0.09953528573706905 2305 2 0.11719541967190139 2305 3 0.10203447518620123 2305 4 0.049502239446951535 2305 5 0.06785762016608743 2305 6 0.07255817239921925 2305 7 0.008694576086874406 2305 8 0.058180132582222654 2305 9 0.05361344878409113 2305 10-11 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-1.3439859207134006 1213 92-93 -1.5175947017485143 1213 94-95 -1.5230116892792331 1213 96-97 -1.5452735772104873 1213 98-99 -1.5907815119591397 1213 100-101 -1.4929419212454214 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 4.0 9 16.0 10 21.0 11 12.0 12 36.0 13 130.0 14 354.0 15 853.0 16 1567.0 17 2473.0 18 3731.0 19 4943.0 20 6543.0 21 8337.0 22 10578.0 23 13248.0 24 17022.0 25 21627.0 26 27420.0 27 36696.0 28 53013.0 29 72330.0 30 69904.0 31 74821.0 32 97816.0 33 135415.0 34 202153.0 35 333774.0 36 606500.0 37 962464.0 38 361307.0 39 1302.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.29339249734685 25.327029853730565 22.05881410502894 16.320763543893648 2 29.089841354831464 24.84036807700587 31.560981585594472 14.508808982568192 3 36.13213361902833 20.206204494546622 26.918341830296782 16.74332005612826 4 28.667576415372576 20.936055925874033 31.81574197940078 18.580625679352607 5 32.51047287128915 19.175629819623843 31.6798719042378 16.634025404849204 6 27.838726258319845 20.35020347612646 35.180883127650205 16.630187137903494 7 31.993298385912794 22.617083934262002 27.163735030359092 18.22588264946611 8 28.463660088196978 30.07103032838613 24.197106522462978 17.26820306095392 9 31.58845717981417 25.207818165941713 24.561997766768346 18.64172688747577 10-11 26.68509612693457 28.994717456702496 27.718734556597425 16.601451859765508 12-13 25.597322297036442 27.942487408085505 27.242291560514598 19.21789873436346 14-15 24.65347646230774 28.38040168103298 27.881666869774957 19.08445498688432 16-17 24.793033019239832 27.63533957298232 28.18507649921819 19.38655090855966 18-19 22.574655091732982 28.098417002754918 29.713031971804092 19.61389593370801 20-21 23.574315724963864 28.062657149044064 29.69980594362034 18.663221182371736 22-23 22.137828327753454 30.92306161921769 28.14556371507137 18.793546337957483 24-25 21.955286748927126 28.064256426938112 29.766463846244147 20.213992977890623 26-27 22.049244325202285 27.949732136945528 28.262311001336677 21.73871253651551 28-29 22.88505893818823 29.814953951991598 28.09609804980855 19.203889060011623 30-31 21.25120465607369 28.464427741586118 29.62510367318948 20.659263929150708 32-33 21.186753756943666 29.99872697479634 28.252859268982867 20.561659999277126 34-35 22.39623964987329 28.6524068652522 28.605500044619852 20.345853440254658 36-37 21.14947458923347 28.582198565703614 29.794803050526625 20.47352379453629 38-39 21.25322401290069 29.988873587027914 28.222049745317573 20.53585265475382 40-41 21.11968948943224 28.369435667387243 30.842289343696482 19.66858549948404 42-43 21.10464986101915 29.732929665134517 29.507601564774035 19.6548189090723 44-45 20.73896694513587 28.348027653725815 30.010126734515147 20.902878666623163 46-47 22.217088185477873 28.213227261895053 28.416813410551807 21.15287114207527 48-49 20.900294852202034 30.00256706148232 29.34755853260048 19.74957955371516 50-51 22.16119035178252 28.314725490130222 29.445638372413086 20.078445785674177 52-53 20.791505328362433 28.52573176017491 28.048778147236497 22.63398476422616 54-55 21.943713216787277 28.4533091435031 29.693027279301525 19.909950360408104 56-57 20.869696108323947 28.287614227115505 29.39002676330099 21.452662901259558 58-59 20.696931076190953 28.625489490012157 29.649269278335872 21.028310155461018 60-61 21.819415903376395 28.454386387566217 29.708444391548156 20.01775331750924 62-63 20.585993243288634 28.703371669987654 29.27149587166279 21.43913921506092 64-65 21.779996212324786 28.590149869094873 29.53623691908451 20.09361699949583 66-67 20.841473521653022 30.78050948980795 27.96176083050956 20.41625615802947 68-69 20.8273964494675 31.082380512463658 27.931228928961353 20.158994109107486 70-71 20.67080583181718 30.95636087492295 28.10178628476413 20.271047008495735 72-73 20.553419243318366 31.153447044970694 27.89270353792822 20.40043017378272 74-75 20.573579788741082 30.717380283172762 28.2649152998178 20.444124628268355 76-77 20.941542418089202 30.205870218367465 28.156686546759012 20.69590081678432 78-79 20.764383457340468 30.190679965614777 28.438306703551554 20.60662987349321 80-81 20.463900270126796 30.07762486746769 28.601835915364216 20.856638947041297 82-83 20.421417610458192 30.15955494461997 28.30285452456177 21.116172920360068 84-85 20.32087160897231 29.92944247318406 28.730468328283226 21.019217589560395 86-87 20.52539629241374 29.679278735089294 28.678771466722253 21.116553505774714 88-89 20.63789196385123 30.113410409839396 28.44967061135339 20.799027014955982 90-91 20.745207704016895 29.465925894170542 28.42620609608074 21.362660305731815 92-93 20.520988095988553 29.9325841474189 28.22722035044852 21.319207406144034 94-95 20.234425949380455 29.9642436347974 28.56021270781347 21.24111770800867 96-97 20.496187706366918 29.927932859988935 28.21199677028121 21.36388266336293 98-99 20.53829518206549 29.917566510528935 28.11243900444958 21.43169930295599 100-101 20.581605944053706 29.595378127059906 28.100341104718506 21.72267482416788 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 70.0 1 49.0 2 47.0 3 85.5 4 81.0 5 55.5 6 46.5 7 52.5 8 61.5 9 56.5 10 56.5 11 64.5 12 72.0 13 85.5 14 98.0 15 121.0 16 183.5 17 273.0 18 409.0 19 662.0 20 1009.5 21 1566.5 22 2447.5 23 3751.0 24 5504.0 25 7731.0 26 10706.0 27 14412.5 28 18726.5 29 23887.0 30 29824.5 31 36803.0 32 45525.0 33 56655.5 34 70111.0 35 86681.5 36 108028.5 37 133430.0 38 158257.0 39 175077.0 40 183440.0 41 181106.5 42 172560.5 43 168297.0 44 162736.0 45 157135.0 46 154392.5 47 144577.0 48 127337.5 49 110939.5 50 98465.5 51 87755.5 52 76266.5 53 64345.0 54 53184.5 55 42957.5 56 33797.0 57 26508.0 58 21028.5 59 16709.0 60 13047.0 61 9952.5 62 7399.5 63 5402.0 64 3969.5 65 2860.5 66 2068.5 67 1491.5 68 1076.0 69 778.0 70 558.5 71 404.5 72 293.0 73 223.0 74 162.5 75 117.5 76 85.5 77 66.0 78 48.0 79 36.0 80 27.0 81 20.0 82 15.0 83 10.0 84 7.0 85 6.0 86 3.0 87 2.0 88 3.0 89 4.5 90 3.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17895919634366692 2 0.0 3 0.0 4 0.008732057301487232 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0024309023989488266 12-13 0.0 14-15 0.0 16-17 2.0790612622588646E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.002127039599080223 40-41 0.006557039365585651 42-43 0.0025908301883533547 44-45 0.0010555234100698852 46-47 0.006908880502275613 48-49 0.008588122291023157 50-51 0.005613465408098935 52-53 0.006908880502275613 54-55 0.0185356307919848 56-57 0.021606244348551743 58-59 0.026755919167377542 60-61 0.02552447518896268 62-63 0.010683176332222474 64-65 0.01544902445647741 66-67 0.022885666663787965 68-69 0.026340106914925773 70-71 0.028834980429636413 72-73 0.022309926621931667 74-75 0.03212949289136969 76-77 0.029506677145135426 78-79 0.020790612622588647 80-81 0.009163862332879458 82-83 0.0018711551360329783 84-85 0.007628555554595989 86-87 0.0270757747461866 88-89 0.024692850684059135 90-91 0.021270395990802233 92-93 0.042540791981604466 94-95 0.043916170970483406 96-97 0.03395266969058131 98-99 0.041117434655904166 100-101 0.04065364406663104 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3126411.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.17591620851833 #Duplication Level Percentage of deduplicated Percentage of total 1 94.6770383402254 73.06787177815912 2 3.1159381489532243 4.809507629890795 3 0.9010301854855416 2.0861349028913367 4 0.2938738698587631 0.907199406243717 5 0.21992787432236954 0.848656760031037 6 0.13957856842647706 0.6463262340832041 7 0.11536274164770291 0.6232257698091646 8 0.08552768680123493 0.5280542072064405 9 0.0536348078979177 0.3725383896170706 >10 0.32484467338307577 4.907194371924084 >50 0.033698029439691086 1.860060750426065 >100 0.037297780195113865 5.75320906460007 >500 0.0018596616750124332 0.9566670596997888 >1k 3.461293240336609E-4 0.3320424247128226 >5k 0.0 0.0 >10k+ 4.15023643442882E-5 2.301311250705367 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 71849 2.298130348185188 TruSeq Adapter, Index 2 (97% over 37bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.316245049035459E-4 0.0 0.0 2 0.0010235378521889797 0.0 0.0 3 0.0013114078731171301 3.1985557880905615E-5 0.0 4 0.0013753789888789414 3.1985557880905615E-5 0.0 5 0.0015992778940452806 3.1985557880905615E-5 0.0 6 0.0019191334728543368 3.1985557880905615E-5 0.0 7 0.002654801304115166 3.1985557880905615E-5 3.1985557880905615E-5 8 0.003422454693256901 3.1985557880905615E-5 3.1985557880905615E-5 9 0.0037742958299468625 3.1985557880905615E-5 3.1985557880905615E-5 10-11 0.005437544839753955 3.1985557880905615E-5 4.797833682135842E-5 12-13 0.006477075470883387 3.1985557880905615E-5 7.996389470226404E-5 14-15 0.007772490565060065 3.1985557880905615E-5 9.595667364271685E-5 16-17 0.009099891217117647 3.1985557880905615E-5 9.595667364271685E-5 18-19 0.010491262984937041 3.1985557880905615E-5 1.1194945258316965E-4 20-21 0.012090540878982321 3.1985557880905615E-5 1.2794223152362246E-4 22-23 0.013785775446670319 3.1985557880905615E-5 1.5992778940452808E-4 24-25 0.015704908919524654 3.1985557880905615E-5 1.5992778940452808E-4 26-27 0.01771999906602171 3.1985557880905615E-5 1.5992778940452808E-4 28-29 0.020022959233446914 3.1985557880905615E-5 1.5992778940452808E-4 30-31 0.03401664080634312 3.1985557880905615E-5 1.5992778940452808E-4 32-33 0.03705526880502916 3.1985557880905615E-5 1.919133472854337E-4 34-35 0.039502163982918434 3.1985557880905615E-5 2.398916841067921E-4 36-37 0.042444835307961754 3.1985557880905615E-5 2.878700209281505E-4 38-39 0.045051658275255556 3.1985557880905615E-5 3.0386279986860333E-4 40-41 0.04901786745248785 3.1985557880905615E-5 3.1985557880905615E-4 42-43 0.05303205496654151 3.1985557880905615E-5 3.5184113668996174E-4 44-45 0.0581177586696055 3.1985557880905615E-5 3.6783391563041456E-4 46-47 0.06336339016207401 3.1985557880905615E-5 4.1581225245177297E-4 48-49 0.06884891333864934 3.1985557880905615E-5 4.1581225245177297E-4 50-51 0.07562985160940133 3.1985557880905615E-5 4.1581225245177297E-4 52-53 0.08221887653286788 3.1985557880905615E-5 4.1581225245177297E-4 54-55 0.09040717935037972 3.1985557880905615E-5 4.477978103326786E-4 56-57 0.09832360492590386 3.1985557880905615E-5 4.477978103326786E-4 58-59 0.10774335172183055 3.1985557880905615E-5 4.477978103326786E-4 60-61 0.11865042695921937 6.397111576181123E-5 4.6379058927313143E-4 62-63 0.13067699672243988 6.397111576181123E-5 4.797833682135842E-4 64-65 0.1451824472214306 6.397111576181123E-5 4.957761471540371E-4 66-67 0.16218277123513192 6.397111576181123E-5 5.117689260944898E-4 68-69 0.18079836592181897 6.397111576181123E-5 5.117689260944898E-4 70-71 0.2008852962710277 6.397111576181123E-5 5.117689260944898E-4 72-73 0.22409081851362472 6.397111576181123E-5 5.277617050349426E-4 74-75 0.25039893987066963 6.397111576181123E-5 5.437544839753954E-4 76-77 0.27971370366851955 6.397111576181123E-5 5.75740041856301E-4 78-79 0.31358640946439864 6.397111576181123E-5 5.75740041856301E-4 80-81 0.3521929778266517 6.397111576181123E-5 5.75740041856301E-4 82-83 0.3929105930090445 6.397111576181123E-5 6.397111576181123E-4 84-85 0.4409209153882839 6.397111576181123E-5 6.397111576181123E-4 86-87 0.49492852987019303 6.397111576181123E-5 6.716967154990179E-4 88-89 0.554757515886427 6.397111576181123E-5 7.036822733799235E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 10430 0.0 74.08682 3 CGGAAGA 10575 0.0 72.84642 4 GATCGGA 10685 0.0 72.039665 1 ATCGGAA 10755 0.0 71.49476 2 GAAGAGC 11705 0.0 65.932396 6 GGAAGAG 15050 0.0 51.530766 5 TTGTGTT 22910 0.0 49.608807 1 AGAGCAC 15740 0.0 48.970066 8 GAGCACA 15925 0.0 48.341537 9 CGGCGAC 40 5.3930306E-4 47.49389 2 AAGAGCA 16955 0.0 45.40484 7 ATGCCGT 8550 0.0 44.77775 48-49 GCCGTCT 8615 0.0 44.771793 50-51 TCGTATG 8590 0.0 44.648254 44-45 CGTATGC 8500 0.0 44.61846 46-47 TGCCGTC 8580 0.0 44.56689 50-51 TATGCCG 8520 0.0 44.51728 48-49 TCTCGTA 8575 0.0 44.448704 42-43 ATCTCGT 8550 0.0 44.1065 42-43 CGTCTTC 8760 0.0 44.060642 52-53 >>END_MODULE