FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_50331580-1.341000000357a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_50331580-1.341000000357a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3126411
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT718492.298130348185188TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG104300.074.086823
CGGAAGA105750.072.846424
GATCGGA106850.072.0396651
ATCGGAA107550.071.494762
GAAGAGC117050.065.9323966
GGAAGAG150500.051.5307665
TTGTGTT229100.049.6088071
AGAGCAC157400.048.9700668
GAGCACA159250.048.3415379
CGGCGAC405.3930306E-447.493892
AAGAGCA169550.045.404847
ATGCCGT85500.044.7777548-49
GCCGTCT86150.044.77179350-51
TCGTATG85900.044.64825444-45
CGTATGC85000.044.6184646-47
TGCCGTC85800.044.5668950-51
TATGCCG85200.044.5172848-49
TCTCGTA85750.044.44870442-43
ATCTCGT85500.044.106542-43
CGTCTTC87600.044.06064252-53
Produced by FastQC (version 0.11.2)