##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_50330665-2.34100000035758.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2522373 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66845268324708 33.0 33.0 33.0 33.0 33.0 2 32.686254570596816 33.0 33.0 33.0 33.0 33.0 3 32.74291589705408 33.0 33.0 33.0 33.0 33.0 4 36.698249228008706 37.0 37.0 37.0 37.0 37.0 5 36.65390566740129 37.0 37.0 37.0 37.0 37.0 6 36.56688562714555 37.0 37.0 37.0 37.0 37.0 7 36.53679729365958 37.0 37.0 37.0 37.0 37.0 8 36.47303313189604 37.0 37.0 37.0 37.0 37.0 9 36.47668960934802 37.0 37.0 37.0 37.0 37.0 10-11 36.4399924198364 37.0 37.0 37.0 37.0 37.0 12-13 36.42420113916538 37.0 37.0 37.0 37.0 37.0 14-15 38.29410023814876 40.0 37.0 40.0 37.0 40.0 16-17 38.295963562883045 40.0 37.0 40.0 37.0 40.0 18-19 38.326503851730095 40.0 37.0 40.0 37.0 40.0 20-21 38.279296321360874 40.0 37.0 40.0 37.0 40.0 22-23 38.19376912137895 40.0 37.0 40.0 37.0 40.0 24-25 38.16747067146691 40.0 37.0 40.0 37.0 40.0 26-27 38.10197996093361 40.0 37.0 40.0 37.0 40.0 28-29 38.00208553612015 40.0 37.0 40.0 37.0 40.0 30-31 37.96984109804537 40.0 37.0 40.0 35.0 40.0 32-33 37.86109488961387 40.0 37.0 40.0 33.0 40.0 34-35 37.79064832996548 40.0 37.0 40.0 33.0 40.0 36-37 37.728710226441535 40.0 37.0 40.0 33.0 40.0 38-39 37.63764716796445 40.0 37.0 40.0 33.0 40.0 40-41 37.55268966960874 40.0 37.0 40.0 33.0 40.0 42-43 37.44717751894744 40.0 37.0 40.0 33.0 40.0 44-45 37.33173523503463 40.0 37.0 40.0 33.0 40.0 46-47 37.29413017028013 40.0 37.0 40.0 33.0 40.0 48-49 37.10547686642697 40.0 37.0 40.0 33.0 40.0 50-51 36.98043608142015 37.0 37.0 40.0 33.0 40.0 52-53 36.87221992940775 37.0 37.0 40.0 33.0 40.0 54-55 36.64173300300946 37.0 37.0 40.0 33.0 40.0 56-57 36.52389932020364 37.0 37.0 40.0 33.0 40.0 58-59 36.33891934301549 37.0 37.0 40.0 33.0 40.0 60-61 36.09297990424096 37.0 37.0 40.0 33.0 40.0 62-63 35.829732755623375 37.0 37.0 40.0 33.0 40.0 64-65 35.61414231757159 37.0 35.0 40.0 30.0 40.0 66-67 35.3077060767777 37.0 33.0 38.5 27.0 40.0 68-69 35.06557396546823 37.0 33.0 37.0 27.0 40.0 70-71 34.70185178797902 37.0 33.0 37.0 27.0 40.0 72-73 34.46468979805921 37.0 33.0 37.0 27.0 40.0 74-75 34.107368141032275 37.0 33.0 37.0 27.0 38.5 76-77 31.501559642447805 33.0 30.0 35.0 24.5 37.0 78-79 33.74191683783485 37.0 33.0 37.0 27.0 37.0 80-81 34.08189094158556 37.0 33.0 37.0 27.0 37.0 82-83 34.05049649675127 37.0 33.0 37.0 27.0 37.0 84-85 33.90271442804058 37.0 33.0 37.0 27.0 37.0 86-87 33.74640745044448 37.0 33.0 37.0 27.0 37.0 88-89 33.53148384477633 37.0 33.0 37.0 27.0 37.0 90-91 33.28479947256017 37.0 33.0 37.0 27.0 37.0 92-93 33.27196849950424 37.0 33.0 37.0 27.0 37.0 94-95 33.20654677163132 37.0 33.0 37.0 27.0 37.0 96-97 33.05003780170498 35.0 33.0 37.0 27.0 37.0 98-99 32.89718768794306 33.0 33.0 37.0 27.0 37.0 100-101 31.377548839921772 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.19319837784016158 2306 2 0.19198115620061174 2306 3 0.12001862814594233 2306 4 0.16826328617736408 2306 5 0.2095696932936164 2306 6 0.14032137859038585 2306 7 0.14825868337445058 2306 8 0.2423812117467179 2306 9 0.2112593597963226 2306 10-11 0.1541086894699788 2306 12-13 0.23394438863653022 2306 14-15 0.3587229824728766 2306 16-17 0.3394934677666228 2306 18-19 0.3163599924251912 2306 20-21 0.4016363355825803 2306 22-23 0.3704680197588104 2306 24-25 0.4497069790029897 2306 26-27 0.47177372153361574 2306 28-29 0.48064426370233804 2306 30-31 0.4537898814266157 2306 32-33 0.3979476759222038 2306 34-35 0.4888202212620172 2306 36-37 0.5177609760669739 2306 38-39 0.36761246553272287 2306 40-41 0.4034726066439944 2306 42-43 0.40682791396837814 2306 44-45 0.42065881187160414 2306 46-47 0.4356359749702605 2306 48-49 0.6248457589305545 2306 50-51 0.43427509387126406 2306 52-53 0.21406490160801184 2306 54-55 0.3822602389017362 2306 56-57 0.4049697925384237 2306 58-59 0.5193655429665327 2306 60-61 0.4638870656765377 2306 62-63 0.450788857761232 2306 64-65 0.4698969437803626 2306 66-67 0.4011692553070034 2306 68-69 0.4957939135999254 2306 70-71 0.5497081347052557 2306 72-73 0.4469310967353195 2306 74-75 0.34554695685960723 2306 76-77 -0.03086914915403227 2306 78-79 0.5322561958261716 2306 80-81 0.4690713990118027 2306 82-83 0.41054733927905573 2306 84-85 0.4724099500270782 2306 86-87 0.5151364830201928 2306 88-89 0.6652524809353011 2306 90-91 0.6322372901822959 2306 92-93 0.4138067360442932 2306 94-95 0.4612460591105858 2306 96-97 0.42031928997005963 2306 98-99 0.26601811974927614 2306 100-101 0.36015919805738505 2307 1 0.13734453462775775 2307 2 0.12161749692532453 2307 3 0.11340071589519596 2307 4 0.08374341071404956 2307 5 0.20791249941008516 2307 6 0.15968844024603612 2307 7 0.13263619550873784 2307 8 0.13951488010371094 2307 9 0.11726072639737595 2307 10-11 0.21535995504249428 2307 12-13 0.15943883098073997 2307 14-15 0.2639282061913377 2307 16-17 0.314511853873789 2307 18-19 0.3004796373696337 2307 20-21 0.2376758486033168 2307 22-23 0.3621713228487664 2307 24-25 0.3482760944710037 2307 26-27 0.340148340775535 2307 28-29 0.37814158047132906 2307 30-31 0.3436535689464435 2307 32-33 0.3030032579117474 2307 34-35 0.36223825561606304 2307 36-37 0.47900241036800395 2307 38-39 0.3118015322897705 2307 40-41 0.44223185129816756 2307 42-43 0.3345335776498146 2307 44-45 0.3531589856841393 2307 46-47 0.40563593151713206 2307 48-49 0.4371805012317438 2307 50-51 0.12083025979705297 2307 52-53 0.1048633746649017 2307 54-55 0.4005007780682206 2307 56-57 0.3371909157584767 2307 58-59 0.3136176710837546 2307 60-61 0.2856398673173288 2307 62-63 0.15563005285276432 2307 64-65 0.2918136452095368 2307 66-67 0.2150696683290434 2307 68-69 0.28065459742189347 2307 70-71 0.24347557027512323 2307 72-73 0.2127282118817746 2307 74-75 0.24314761061200585 2307 76-77 0.1857318218062396 2307 78-79 0.2855860637720937 2307 80-81 0.4122406750391363 2307 82-83 0.30153293239261103 2307 84-85 0.34195753229472814 2307 86-87 0.3287945090312334 2307 88-89 0.4315560966705476 2307 90-91 0.37285005540935856 2307 92-93 0.3895746887064462 2307 94-95 0.3495439401185152 2307 96-97 0.2136443726396422 2307 98-99 0.12675462863762021 2307 100-101 0.10478852653265491 2201 1 0.15773071348954915 2201 2 0.18406902913295653 2201 3 0.10912304234923198 2201 4 0.12082752578596967 2201 5 0.16402569515742726 2201 6 0.1265177085387137 2201 7 0.1067262517939156 2201 8 0.07305615590108516 2201 9 0.10972007599087163 2201 10-11 0.16240029275355994 2201 12-13 0.1560695376724155 2201 14-15 0.3083426839899559 2201 16-17 0.4073394023415773 2201 18-19 0.28657172617513993 2201 20-21 0.33426512314990475 2201 22-23 0.3011214479269384 2201 24-25 0.3168697467536745 2201 26-27 0.3437638817386386 2201 28-29 0.38964877246633023 2201 30-31 0.34230897857746356 2201 32-33 0.4167438770487095 2201 34-35 0.3371366295998115 2201 36-37 0.22947458047432434 2201 38-39 0.1930101001446829 2201 40-41 0.27797684817121393 2201 42-43 0.26518242067670883 2201 44-45 0.22013365735393364 2201 46-47 0.25807495590737517 2201 48-49 0.42255026358321146 2201 50-51 0.3514181272141883 2201 52-53 0.3351604353278148 2201 54-55 0.32031941471612413 2201 56-57 0.42608944608993937 2201 58-59 0.37923055413565976 2201 60-61 0.42735812873071666 2201 62-63 0.36386879581711895 2201 64-65 0.2993816564665721 2201 66-67 0.38825212611516235 2201 68-69 0.4110165423875003 2201 70-71 0.401002931125646 2201 72-73 0.3278986308936638 2201 74-75 0.30367684763513836 2201 76-77 0.1899946423190606 2201 78-79 0.41336748966327974 2201 80-81 0.3855208501485805 2201 82-83 0.4656401869266844 2201 84-85 0.44311997757302635 2201 86-87 0.46988425262097877 2201 88-89 0.37063051818399373 2201 90-91 0.37096606541560817 2201 92-93 0.4771802234156439 2201 94-95 0.3591945029703041 2201 96-97 0.5250554107884895 2201 98-99 0.4166389313268013 2201 100-101 0.5427286453569202 1101 1 0.11356817632403704 1101 2 0.15033062350158843 1101 3 0.09036011383407327 1101 4 0.04520962535141848 1101 5 0.07667594546872891 1101 6 0.09774995168374545 1101 7 0.18929899454504806 1101 8 0.15007884909820746 1101 9 0.13144455567373114 1101 10-11 0.15863622068424377 1101 12-13 0.18824915191348168 1101 14-15 0.32272097170818626 1101 16-17 0.25957060358170025 1101 18-19 0.2712984167293797 1101 20-21 0.2556404376273065 1101 22-23 0.24030596674475646 1101 24-25 0.2286064243476389 1101 26-27 0.2231316233462266 1101 28-29 0.2326743626673391 1101 30-31 0.21971616009762585 1101 32-33 0.308489703075999 1101 34-35 0.18446071285055154 1101 36-37 0.2655887834170727 1101 38-39 0.29817871085342773 1101 40-41 0.3674841033870919 1101 42-43 0.338050200402769 1101 44-45 0.3899382366530588 1101 46-47 0.41445090569263954 1101 48-49 0.48377361241805517 1101 50-51 0.4341405484995491 1101 52-53 0.4016929249722949 1101 54-55 0.5471444001040453 1101 56-57 0.4344102003274415 1101 58-59 0.5478359262990082 1101 60-61 0.5858399970440971 1101 62-63 0.5674438002642717 1101 64-65 0.5495803807683686 1101 66-67 0.34280093046574933 1101 68-69 0.4536451922312068 1101 70-71 0.5048752596560604 1101 72-73 0.5163810074533401 1101 74-75 0.4939294460443264 1101 76-77 0.2843546331706257 1101 78-79 0.5786240269812595 1101 80-81 0.6166560182510423 1101 82-83 0.6261817233552378 1101 84-85 0.6053277994835256 1101 86-87 0.5779066662826366 1101 88-89 0.5183098655957465 1101 90-91 0.6277968956359317 1101 92-93 0.4869334698388599 1101 94-95 0.44050501604493775 1101 96-97 0.48947918655021994 1101 98-99 0.5056303165520788 1101 100-101 0.4698870403151183 2304 1 0.03867146428104462 2304 2 0.10735400286569075 2304 3 0.05171358613380761 2304 4 0.038859046504057915 2304 5 0.04681802720348571 2304 6 0.01596139917644024 2304 7 0.06053274380936102 2304 8 0.0820865285135497 2304 9 0.04693400633913569 2304 10-11 0.08302757591816601 2304 12-13 0.08569240236990794 2304 14-15 0.23002448477624426 2304 16-17 0.18145657869755638 2304 18-19 0.19685040272247534 2304 20-21 0.1448614422536849 2304 22-23 0.17023914614911462 2304 24-25 0.21734986365170528 2304 26-27 0.11030704007284697 2304 28-29 0.10985717103293524 2304 30-31 0.14335792962641847 2304 32-33 0.09858362933622544 2304 34-35 0.20452695072147264 2304 36-37 0.0859992995363612 2304 38-39 0.12463936640082096 2304 40-41 0.10223318884546728 2304 42-43 0.060412911578843875 2304 44-45 0.11860076238819772 2304 46-47 0.0599971630534597 2304 48-49 0.23586480973357737 2304 50-51 0.12061514909778026 2304 52-53 0.20953541358338867 2304 54-55 0.2728546406841872 2304 56-57 0.15039588591876196 2304 58-59 0.21094505853672985 2304 60-61 0.20186183398069346 2304 62-63 0.22194171791988282 2304 64-65 0.17298601230974242 2304 66-67 0.0293943172573492 2304 68-69 0.11623659596575919 2304 70-71 0.24758783875533652 2304 72-73 0.15535088013695741 2304 74-75 0.18019773967841957 2304 76-77 -0.043901262770877025 2304 78-79 0.2645114203594474 2304 80-81 0.35091068419513505 2304 82-83 0.26676428670750596 2304 84-85 0.31466157030865105 2304 86-87 0.2688951422288852 2304 88-89 0.418039974399413 2304 90-91 0.5201119444122426 2304 92-93 0.37217726617387825 2304 94-95 0.2060337333916209 2304 96-97 0.21550073660694835 2304 98-99 0.22740851000682483 2304 100-101 0.2853522406670841 2202 1 0.09380985018019317 2202 2 0.14580302655040356 2202 3 0.1211798584348287 2202 4 0.057854365852378464 2202 5 0.13627712478295706 2202 6 0.13073233683607555 2202 7 0.06634532576550356 2202 8 0.2055169564913797 2202 9 0.12820928462397063 2202 10-11 0.10343607950877498 2202 12-13 0.15249316799892654 2202 14-15 0.26236177640833347 2202 16-17 0.2185757978405647 2202 18-19 0.18579211909157323 2202 20-21 0.16654860405748906 2202 22-23 0.21524130588672108 2202 24-25 0.22759885577415417 2202 26-27 0.20156225842190878 2202 28-29 0.21810984975834202 2202 30-31 0.22502895644132082 2202 32-33 0.3007221559640456 2202 34-35 0.33722655765735965 2202 36-37 0.38166259929004553 2202 38-39 0.35201143753631214 2202 40-41 0.182741882297762 2202 42-43 0.32189612663501777 2202 44-45 0.32059113752363544 2202 46-47 0.2621367065068938 2202 48-49 0.3200345838193286 2202 50-51 0.2520137276569159 2202 52-53 0.32750294593471097 2202 54-55 0.4270679389736429 2202 56-57 0.46548462225668885 2202 58-59 0.3917563796291077 2202 60-61 0.37814695920479835 2202 62-63 0.30641491276970356 2202 64-65 0.26413700603307433 2202 66-67 0.3028573650009747 2202 68-69 0.2941017665158867 2202 70-71 0.4844663142652905 2202 72-73 0.26121259510690464 2202 74-75 0.37236435916437927 2202 76-77 0.23167007120671812 2202 78-79 0.4367529351530308 2202 80-81 0.4341615529709415 2202 82-83 0.39694806370219027 2202 84-85 0.3653315160345656 2202 86-87 0.26367160735258466 2202 88-89 0.4588105126499542 2202 90-91 0.6702743111267324 2202 92-93 0.48215854844792716 2202 94-95 0.4718570652281855 2202 96-97 0.26966480020004013 2202 98-99 0.21455329214583685 2202 100-101 0.3150026722892214 1102 1 0.08075966671894719 1102 2 0.10137015414009198 1102 3 0.05684504566143289 1102 4 0.00834165975889789 1102 5 0.09730763222555083 1102 6 0.09557487260701691 1102 7 0.10680734367901579 1102 8 0.04975194711803255 1102 9 0.13871784881938254 1102 10-11 0.07028847732325971 1102 12-13 0.09721224796880534 1102 14-15 0.1882296122065057 1102 16-17 0.15153733506957678 1102 18-19 0.11116655228443761 1102 20-21 0.15314982419496204 1102 22-23 0.15190381329728098 1102 24-25 0.2316376069558359 1102 26-27 0.23713090817916083 1102 28-29 0.23708466990222377 1102 30-31 0.31900077230712043 1102 32-33 0.25619565021985835 1102 34-35 0.2302323979739711 1102 36-37 0.274448025737307 1102 38-39 0.13920049475378704 1102 40-41 0.21282922667615622 1102 42-43 0.32143106438638114 1102 44-45 0.2483490648121034 1102 46-47 0.28416998189104703 1102 48-49 0.36821298986954787 1102 50-51 0.30438540492534827 1102 52-53 0.23176371326739797 1102 54-55 0.22150952379041655 1102 56-57 0.21476970421339558 1102 58-59 0.1833702377630928 1102 60-61 0.17784182469325316 1102 62-63 0.11603200751262222 1102 64-65 0.0930296491568825 1102 66-67 0.2684103123980677 1102 68-69 0.2859625763090321 1102 70-71 0.1527207085227147 1102 72-73 0.21272074270908803 1102 74-75 0.28839959333784293 1102 76-77 -0.07255975206161835 1102 78-79 0.3752263212548499 1102 80-81 0.32257755736119265 1102 82-83 0.24026014009898233 1102 84-85 0.27748352048890723 1102 86-87 0.3355224229139395 1102 88-89 0.47022106127119656 1102 90-91 0.5926381572436057 1102 92-93 0.347193358016753 1102 94-95 0.19712494669139602 1102 96-97 0.18950004558947597 1102 98-99 0.06514843963586259 1102 100-101 0.12009772764804794 2305 1 0.06052732365903779 2305 2 0.06785216689914364 2305 3 0.035431169250578876 2305 4 0.039791019918951065 2305 5 0.12620518233691058 2305 6 0.06773239780425655 2305 7 0.04886879851272852 2305 8 0.08935124499278402 2305 9 -0.012822296890483642 2305 10-11 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-1.5763288335041885 1213 98-99 -1.4679261417854477 1213 100-101 -1.4715443355818003 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 8.0 9 24.0 10 21.0 11 9.0 12 20.0 13 54.0 14 140.0 15 421.0 16 731.0 17 1272.0 18 1991.0 19 2701.0 20 3625.0 21 4740.0 22 6072.0 23 7786.0 24 10006.0 25 12703.0 26 16493.0 27 21668.0 28 29339.0 29 35515.0 30 39623.0 31 49540.0 32 67460.0 33 95673.0 34 147334.0 35 258585.0 36 531075.0 37 892440.0 38 284945.0 39 359.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.98029330852685 25.98833343528139 23.645050766159496 13.38632249003226 2 31.372719260791328 22.803566324251012 33.676779762548996 12.146934652408664 3 38.54866032898386 18.85708418223633 27.01404590042789 15.58020958835192 4 30.64641086822233 18.94821941032662 34.6837331125686 15.72163660888245 5 30.625288171099196 17.514657824199674 36.462331304688085 15.397722700013045 6 25.46157923510916 18.303874962188385 40.77204283426757 15.462502968434883 7 34.114542139485316 19.05408121637839 29.778823354040025 17.05255329009627 8 30.215951407662544 28.688778384481594 24.86170760628979 16.233562601566064 9 29.80776435523216 26.619536444451313 25.778820182423456 17.793879017893072 10-11 24.182832403630005 29.005415670555955 30.852333773406126 15.959418152407912 12-13 24.21362740562161 27.730018518276246 30.00886466831036 18.04748940779179 14-15 22.8889422777678 28.441550872927994 31.00156083180402 17.66794601750019 16-17 21.76110340781032 29.129260506250574 32.01113636296059 17.09849972297851 18-19 20.477007167456993 29.377950842321894 32.350528649014244 17.794513341206873 20-21 21.03227793827479 29.40764510244916 31.828500384360282 17.731576574915763 22-23 20.341261978303766 30.171885759956997 31.6358643230006 17.85098793873864 24-25 20.395833605894133 29.204126431737098 31.81226567204771 18.587774290321057 26-27 20.249344565613413 29.33277512881719 31.084003039994478 19.333877265574916 28-29 20.345979757950154 29.917541933726692 30.794731786298062 18.941746522025092 30-31 19.467104984076503 30.146532650008545 30.940903664921883 19.445458700993072 32-33 19.22701360980315 30.869760340758486 31.13805927989239 18.765166769545978 34-35 19.804287470568386 30.052256347495 31.73711818196595 18.406337999970663 36-37 20.158101121443973 30.299404568634376 31.40909373831705 18.133400571604597 38-39 20.660799064355306 30.544794980821266 30.64932155848275 18.14508439634068 40-41 20.352061482795882 30.247305776322786 30.596128025457126 18.8045047154242 42-43 19.812461518394457 30.687199704715006 31.272615686658533 18.227723090232008 44-45 19.57607381530593 30.197777905084127 31.547015986484027 18.679132293125914 46-47 20.279637933700474 29.89195897216308 30.881257954396773 18.94714513973967 48-49 19.998287167814137 30.781279457888612 30.568464023684662 18.651969350612585 50-51 20.456721553129224 30.13249746157875 30.211256912018975 19.19952407327305 52-53 19.90672026805186 29.943995288237623 30.01986164054891 20.129422803161606 54-55 20.62897423655798 30.01810562646419 30.22638377583601 19.12653636114182 56-57 20.1362356437269 30.313382427759144 30.13706044911447 19.413321479399496 58-59 20.134189995155946 30.33654373319676 30.593974536895207 18.935291734752088 60-61 20.369524014222925 30.739260186615912 30.272036343685926 18.619179455475237 62-63 19.84600921692606 30.618167645282757 30.20293810829789 19.33288502949329 64-65 20.335542074800088 30.240024679325394 30.338400974801633 19.086032271072888 66-67 20.37675079553 30.45119721589779 29.87941095585936 19.292641032712844 68-69 20.37740166362403 30.712662494420844 29.988537328632468 18.921398513322654 70-71 19.73663364491207 31.340362278939278 30.03560607157183 18.88739800457682 72-73 19.594372608948664 31.39615092162358 29.955913945449087 19.05356252397867 74-75 20.084536076750318 30.680317918467658 29.765197834486802 19.469948170295222 76-77 20.140917737971687 30.284960459832654 29.997921965027942 19.576199837167717 78-79 19.917920537293536 30.409848107986896 30.300780248585895 19.371451106133676 80-81 19.35940986329993 30.67519385915264 30.193318934114156 19.772077343433274 82-83 18.80554783008259 30.583972411108107 30.15924429874962 20.45123546005968 84-85 18.85588974095516 30.399681702597704 30.614972546452567 20.12945600999457 86-87 18.976202590276806 30.398455621116398 31.158649001590614 19.466692787016175 88-89 19.19618337895719 30.770446368273554 30.692661756500655 19.3407084962686 90-91 19.469890344155104 30.397545561720023 30.515021220961703 19.617542873163167 92-93 18.856513456387564 31.50323315824607 30.362713433462147 19.277539951904217 94-95 18.644689988626258 31.66848957274585 30.309728765457926 19.377091673169964 96-97 19.156957064382603 31.69494577177705 30.0267542028013 19.12134296103904 98-99 19.4122873121009 30.95276246380835 29.784634910562012 19.850315313528736 100-101 19.24199210484462 30.96413229243884 29.542520444866977 20.251355157849563 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 98.0 1 78.0 2 65.5 3 174.5 4 231.0 5 160.5 6 126.0 7 129.5 8 147.0 9 143.5 10 150.5 11 159.5 12 152.5 13 160.5 14 183.0 15 210.5 16 233.0 17 290.0 18 422.0 19 650.5 20 997.5 21 1574.0 22 2512.0 23 3904.5 24 5736.5 25 8279.5 26 12093.0 27 17393.0 28 23213.0 29 29182.5 30 35845.5 31 42573.5 32 51250.5 33 63841.5 34 79889.5 35 99718.5 36 127690.5 37 158835.5 38 184428.0 39 193842.0 40 186675.0 41 169602.0 42 151097.5 43 140656.0 44 126689.0 45 110864.5 46 103758.0 47 92820.0 48 72363.0 49 53999.5 50 40464.5 51 32030.0 52 26491.0 53 20250.0 54 14640.5 55 10287.5 56 7020.0 57 4830.0 58 3459.5 59 2430.0 60 1666.0 61 1135.0 62 792.5 63 532.5 64 342.5 65 229.0 66 152.5 67 94.0 68 62.5 69 52.0 70 40.5 71 32.0 72 22.5 73 12.5 74 15.5 75 14.5 76 5.0 77 5.0 78 6.5 79 5.5 80 4.0 81 0.5 82 1.0 83 1.5 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18835437899152901 2 0.0 3 0.0 4 0.009158042842989518 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0024976480480880504 12-13 0.0 14-15 0.0 16-17 2.1804863911879804E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0019029699414004194 40-41 0.006799153019795248 42-43 0.002537293255200559 44-45 9.316623671439552E-4 46-47 0.007255072901589099 48-49 0.009296801067883298 50-51 0.005391748167301188 52-53 0.006918088641132775 54-55 0.020020829591816912 56-57 0.022439187225679946 58-59 0.028722952553012576 60-61 0.026542466161824596 62-63 0.010862786748827393 64-65 0.016809567815703703 66-67 0.02483772225598672 68-69 0.02812827444632495 70-71 0.029833018352162826 72-73 0.023806946871061496 74-75 0.03353984521718239 76-77 0.03013035740550664 78-79 0.021745396101211043 80-81 0.009732898346120896 82-83 0.0021210185805192175 84-85 0.008067799647395528 86-87 0.02822738746410622 88-89 0.026403707936930816 90-91 0.022756348882580015 92-93 0.045750569007835085 94-95 0.04818874924525437 96-97 0.03568068640125786 98-99 0.042539307231721876 100-101 0.042816823681509436 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2522373.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.29997404031483 #Duplication Level Percentage of deduplicated Percentage of total 1 91.90978954633778 54.502481341486295 2 3.8732760795257595 4.5937034193369986 3 1.206258348517633 2.14593266259026 4 0.6445655554719917 1.5289088282708083 5 0.38061556087558995 1.1285246439631178 6 0.3144890560050059 1.1189515714235978 7 0.23105706390869296 0.9591174524131799 8 0.14396433915239354 0.6829665259574413 9 0.1368813277201962 0.7305353262370315 >10 0.9449822417878624 10.844946977569943 >50 0.09339640235583987 3.831896966486048 >100 0.11410072798860177 13.53883407764993 >500 0.005472241056185764 2.193365607583166 >1k 9.501532255289857E-4 0.8610758539851509 >5k 1.3423738068609506E-4 0.564781382823826 >10k+ 6.711869034017697E-5 0.7739773622233348 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 19446 0.770940697509845 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7537 0.29880592600697836 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6653 0.26375956291952063 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3589 0.14228664832679386 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.1716165690006987E-4 0.0 0.0 2 3.1716165690006987E-4 0.0 0.0 3 3.5680686401257864E-4 0.0 0.0 4 4.360972782375961E-4 0.0 0.0 5 5.94678106687631E-4 0.0 0.0 6 6.343233138001397E-4 0.0 0.0 7 6.739685209126486E-4 0.0 0.0 8 9.11839763587701E-4 0.0 0.0 9 0.0011497110062627534 0.0 0.0 10-11 0.0017642117165066386 0.0 0.0 12-13 0.0023390672196380155 0.0 0.0 14-15 0.0032112617761132075 0.0 0.0 16-17 0.004202391953925926 0.0 0.0 18-19 0.005471038581526206 0.0 0.0 20-21 0.007334363315814116 0.0 0.0 22-23 0.0090787524287645 0.0 1.9822603556254367E-5 24-25 0.01125923881995248 0.0 3.9645207112508734E-5 26-27 0.013717241660928022 0.0 7.929041422501747E-5 28-29 0.016036486277009782 0.0 1.1893562133752621E-4 30-31 0.024203398942186583 0.0 1.1893562133752621E-4 32-33 0.027275902493406008 0.0 1.5858082845003494E-4 34-35 0.030784503322863033 0.0 1.5858082845003494E-4 36-37 0.03476884663767016 3.9645207112508734E-5 1.5858082845003494E-4 38-39 0.038951415988039834 5.94678106687631E-5 1.5858082845003494E-4 40-41 0.043926889480659675 7.929041422501747E-5 1.5858082845003494E-4 42-43 0.04872395954127324 7.929041422501747E-5 1.5858082845003494E-4 44-45 0.05465091800459329 7.929041422501747E-5 1.5858082845003494E-4 46-47 0.06024089220745703 7.929041422501747E-5 1.5858082845003494E-4 48-49 0.06587051161743326 7.929041422501747E-5 1.5858082845003494E-4 50-51 0.0724119707909972 7.929041422501747E-5 1.5858082845003494E-4 52-53 0.07936970463924249 7.929041422501747E-5 1.5858082845003494E-4 54-55 0.0859706316234752 7.929041422501747E-5 1.5858082845003494E-4 56-57 0.0938798504424207 7.929041422501747E-5 1.5858082845003494E-4 58-59 0.10250268298939133 7.929041422501747E-5 1.5858082845003494E-4 60-61 0.11197788748928092 7.929041422501747E-5 1.784034320062893E-4 62-63 0.12236493175275821 7.929041422501747E-5 1.9822603556254368E-4 64-65 0.13306913767313558 9.911301778127184E-5 1.9822603556254368E-4 66-67 0.14405086004330048 1.1893562133752621E-4 1.9822603556254368E-4 68-69 0.15634087424817822 1.1893562133752621E-4 1.9822603556254368E-4 70-71 0.17057350360156884 1.1893562133752621E-4 2.576938462313068E-4 72-73 0.18674874810347242 1.1893562133752621E-4 2.7751644978756115E-4 74-75 0.20391512278318868 1.1893562133752621E-4 2.7751644978756115E-4 76-77 0.22514513119193713 1.1893562133752621E-4 2.7751644978756115E-4 78-79 0.24944764315190499 1.1893562133752621E-4 2.7751644978756115E-4 80-81 0.27434483321856046 1.1893562133752621E-4 2.7751644978756115E-4 82-83 0.3017000261261915 1.1893562133752621E-4 3.1716165690006987E-4 84-85 0.337896100219912 1.1893562133752621E-4 3.1716165690006987E-4 86-87 0.378889244374246 1.1893562133752621E-4 3.1716165690006987E-4 88-89 0.4248182168140874 1.1893562133752621E-4 3.1716165690006987E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 4110 0.0 54.079792 4 TCGGAAG 4110 0.0 54.079792 3 GATCGGA 4215 0.0 53.416714 1 ATCGGAA 4260 0.0 52.175575 2 GAAGAGC 4660 0.0 47.696983 6 CGATCGG 70 7.795097E-8 47.60073 1 GCGCAAC 65 2.5931859E-6 43.83983 2 TCGGACG 55 4.9692328E-5 43.175594 3 TATGCCG 2510 0.0 42.958786 48-49 TGCCGTC 2515 0.0 42.684933 50-51 GCCGTCT 2545 0.0 42.36842 50-51 ATGCCGT 2535 0.0 42.06668 48-49 CGTATGC 2595 0.0 41.822495 46-47 CGCGGGG 80 2.2266977E-7 41.650642 1 GCACGGG 80 2.2266977E-7 41.650642 1 AATCTCG 2590 0.0 41.259758 40-41 CTCGTAT 2605 0.0 40.74991 44-45 TCGTATG 2670 0.0 40.736248 44-45 CCGTCTT 2655 0.0 40.704514 52-53 TCTCGTA 2640 0.0 40.38837 42-43 >>END_MODULE