FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_50330665-2.34100000035758.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_50330665-2.34100000035758.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522373
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT194460.770940697509845TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC75370.29880592600697836No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG66530.26375956291952063No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA35890.14228664832679386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA41100.054.0797924
TCGGAAG41100.054.0797923
GATCGGA42150.053.4167141
ATCGGAA42600.052.1755752
GAAGAGC46600.047.6969836
CGATCGG707.795097E-847.600731
GCGCAAC652.5931859E-643.839832
TCGGACG554.9692328E-543.1755943
TATGCCG25100.042.95878648-49
TGCCGTC25150.042.68493350-51
GCCGTCT25450.042.3684250-51
ATGCCGT25350.042.0666848-49
CGTATGC25950.041.82249546-47
CGCGGGG802.2266977E-741.6506421
GCACGGG802.2266977E-741.6506421
AATCTCG25900.041.25975840-41
CTCGTAT26050.040.7499144-45
TCGTATG26700.040.73624844-45
CCGTCTT26550.040.70451452-53
TCTCGTA26400.040.3883742-43
Produced by FastQC (version 0.11.2)