##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_50330665-1.341000000356c2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2852210 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64180898320951 33.0 33.0 33.0 33.0 33.0 2 32.634281486987284 33.0 33.0 33.0 33.0 33.0 3 32.70865434172098 33.0 33.0 33.0 33.0 33.0 4 36.631613731106754 37.0 37.0 37.0 37.0 37.0 5 36.603527440125376 37.0 37.0 37.0 37.0 37.0 6 36.52582103000831 37.0 37.0 37.0 37.0 37.0 7 36.34713783346948 37.0 37.0 37.0 37.0 37.0 8 36.30438712437022 37.0 37.0 37.0 37.0 37.0 9 36.391707482969345 37.0 37.0 37.0 37.0 37.0 10-11 36.29401201173826 37.0 37.0 37.0 35.0 37.0 12-13 36.29501369113775 37.0 37.0 37.0 35.0 37.0 14-15 38.08807643897188 40.0 37.0 40.0 35.0 40.0 16-17 38.096881891585824 40.0 37.0 40.0 37.0 40.0 18-19 38.04898780244091 40.0 37.0 40.0 37.0 40.0 20-21 38.083706143657025 40.0 37.0 40.0 37.0 40.0 22-23 37.75933591846322 40.0 37.0 40.0 33.0 40.0 24-25 37.94648307803422 40.0 37.0 40.0 33.0 40.0 26-27 37.87517679273265 40.0 37.0 40.0 33.0 40.0 28-29 37.60385017232251 40.0 37.0 40.0 33.0 40.0 30-31 37.54095157789924 40.0 37.0 40.0 33.0 40.0 32-33 37.486252239491485 40.0 37.0 40.0 33.0 40.0 34-35 37.32286349883073 40.0 37.0 40.0 33.0 40.0 36-37 37.29054452512263 40.0 37.0 40.0 33.0 40.0 38-39 37.26185308935878 40.0 37.0 40.0 33.0 40.0 40-41 37.19412999042848 40.0 37.0 40.0 33.0 40.0 42-43 36.50608756017264 37.0 37.0 40.0 33.0 40.0 44-45 36.31794748633516 37.0 37.0 40.0 33.0 40.0 46-47 36.75298102173403 37.0 37.0 40.0 33.0 40.0 48-49 36.41471718421855 37.0 37.0 40.0 33.0 40.0 50-51 36.410845800274174 37.0 37.0 40.0 33.0 40.0 52-53 36.42467630363823 37.0 37.0 40.0 33.0 40.0 54-55 35.94722916615537 37.0 37.0 40.0 30.0 40.0 56-57 35.87985334179461 37.0 37.0 40.0 33.0 40.0 58-59 35.77091728869894 37.0 35.0 40.0 33.0 40.0 60-61 35.48113690787144 37.0 33.0 40.0 27.0 40.0 62-63 35.04708033419699 37.0 33.0 37.0 27.0 40.0 64-65 34.76781408101087 37.0 33.0 37.0 27.0 40.0 66-67 33.884820192061596 37.0 33.0 37.0 27.0 40.0 68-69 33.68797160798118 37.0 33.0 37.0 27.0 37.0 70-71 33.1014942798742 37.0 33.0 37.0 24.5 37.0 72-73 31.820498841249417 33.0 30.0 37.0 18.5 37.0 74-75 30.617747466000047 33.0 27.0 37.0 15.0 37.0 76-77 28.108268325263566 30.0 24.5 33.0 15.0 35.0 78-79 29.957238422135816 33.0 27.0 37.0 15.0 37.0 80-81 30.238494711118747 33.0 27.0 37.0 15.0 37.0 82-83 30.081158470098625 33.0 27.0 37.0 6.0 37.0 84-85 29.912568499514414 33.0 27.0 37.0 6.0 37.0 86-87 29.753947114693517 33.0 27.0 37.0 6.0 37.0 88-89 29.575230961254604 33.0 27.0 37.0 6.0 37.0 90-91 29.324866857629697 33.0 27.0 37.0 6.0 37.0 92-93 29.242823635005838 33.0 27.0 37.0 6.0 37.0 94-95 29.11874160738515 33.0 27.0 37.0 6.0 37.0 96-97 28.935266337331402 33.0 27.0 37.0 6.0 37.0 98-99 28.767264156566313 33.0 27.0 37.0 6.0 37.0 100-101 27.290996630682873 33.0 21.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.15878867826472032 2306 2 0.13717644722296995 2306 3 0.11015227231354174 2306 4 0.15042006347996306 2306 5 0.1365498278187829 2306 6 0.17903723038524078 2306 7 0.1950127666961734 2306 8 0.11623853929374661 2306 9 0.2413870668146174 2306 10-11 0.19314992106726692 2306 12-13 0.18349147675431254 2306 14-15 0.46547004783924706 2306 16-17 0.4341566832868722 2306 18-19 0.4526282443833196 2306 20-21 0.4216112968059136 2306 22-23 0.4197948756487264 2306 24-25 0.3758578002333479 2306 26-27 0.449998503194351 2306 28-29 0.5522793860058073 2306 30-31 0.5147078213077734 2306 32-33 0.5003755040032658 2306 34-35 0.49000391421689926 2306 36-37 0.5677376781193786 2306 38-39 0.5914890531041337 2306 40-41 0.5020916621359248 2306 42-43 0.41564814319285404 2306 44-45 0.6408208903288326 2306 46-47 0.6412075265000681 2306 48-49 0.7138623773194226 2306 50-51 0.6975433541759628 2306 52-53 0.4398015876157828 2306 54-55 0.5299546345601414 2306 56-57 0.4588514679764657 2306 58-59 0.4765478527840017 2306 60-61 0.4122727987989663 2306 62-63 0.558556595963168 2306 64-65 0.474885009450567 2306 66-67 0.4771840374548688 2306 68-69 0.4573569350805613 2306 70-71 0.4879353053731492 2306 72-73 0.05800913819923892 2306 74-75 0.03789864651768937 2306 76-77 -0.17861678674091763 2306 78-79 0.5328100486394014 2306 80-81 0.7037307956023646 2306 82-83 0.5642654375626428 2306 84-85 0.7018487026577809 2306 86-87 0.6343015044414635 2306 88-89 0.7586077623972116 2306 90-91 0.6790515792733167 2306 92-93 0.622553106283565 2306 94-95 0.6233160132469173 2306 96-97 0.6102221295019028 2306 98-99 0.6421203943054721 2306 100-101 0.5766567412165635 2307 1 0.15802553044912315 2307 2 0.18204506653892594 2307 3 0.1410629123485876 2307 4 0.13851358148245652 2307 5 0.21409103550736575 2307 6 0.16593127037854316 2307 7 0.01169044195642499 2307 8 0.1579834701804188 2307 9 0.2015531589703059 2307 10-11 0.2187104992936426 2307 12-13 0.22733893434256203 2307 14-15 0.428096159305845 2307 16-17 0.42275648192135407 2307 18-19 0.4306853078868542 2307 20-21 0.3762879996403967 2307 22-23 0.42073289956029924 2307 24-25 0.37657794573025427 2307 26-27 0.43389241475406237 2307 28-29 0.4621106907764272 2307 30-31 0.44144018405011565 2307 32-33 0.3574925597549097 2307 34-35 0.4139339591915885 2307 36-37 0.42885882310485357 2307 38-39 0.4016404097986239 2307 40-41 0.4526074606144448 2307 42-43 0.4217246575051732 2307 44-45 0.48810990948607724 2307 46-47 0.418604877098538 2307 48-49 0.5633255937093864 2307 50-51 0.44360577519503863 2307 52-53 0.3434176239257951 2307 54-55 0.5959924353183581 2307 56-57 0.4599428668246901 2307 58-59 0.46970991074883983 2307 60-61 0.4856095264486555 2307 62-63 0.3284035994469292 2307 64-65 0.34181920787587927 2307 66-67 0.32588439271135883 2307 68-69 0.3523200448247721 2307 70-71 0.28176269521267017 2307 72-73 0.1210058149800517 2307 74-75 0.08408170337478182 2307 76-77 0.13683904930442736 2307 78-79 0.45867204445154997 2307 80-81 0.7111337881050552 2307 82-83 0.43670221268689247 2307 84-85 0.573644743233622 2307 86-87 0.4872092065580951 2307 88-89 0.6733817536774893 2307 90-91 0.6953124760608738 2307 92-93 0.578687301039345 2307 94-95 0.41696336671221346 2307 96-97 0.44929942588305494 2307 98-99 0.47874025151483934 2307 100-101 0.575251138759814 2201 1 0.11198234812505348 2201 2 0.13339299343951438 2201 3 0.12851770734564383 2201 4 0.14603655909646562 2201 5 0.22001479461708584 2201 6 0.12781641249775788 2201 7 0.1688101904936019 2201 8 0.0887302617854715 2201 9 0.11000623543378651 2201 10-11 0.20515643307378184 2201 12-13 0.1281034880329841 2201 14-15 0.270738411440945 2201 16-17 0.2816204474173034 2201 18-19 0.16501416510257627 2201 20-21 0.19447940300735667 2201 22-23 0.32829715081766153 2201 24-25 0.25844331615219573 2201 26-27 0.38991561811146624 2201 28-29 0.31041545247520475 2201 30-31 0.3417679400345648 2201 32-33 0.3347862884140511 2201 34-35 0.3773529848993036 2201 36-37 0.30366270571106924 2201 38-39 0.20244515572690602 2201 40-41 0.3866225300001247 2201 42-43 0.45922762010565776 2201 44-45 0.4721745883491977 2201 46-47 0.4568419338011367 2201 48-49 0.5990534291771397 2201 50-51 0.4895337878330608 2201 52-53 0.30145179093265284 2201 54-55 0.4601837647892708 2201 56-57 0.48259416671916 2201 58-59 0.4964509060203923 2201 60-61 0.38874524193808213 2201 62-63 0.46823807231131553 2201 64-65 0.4026994539316817 2201 66-67 0.4182884202259203 2201 68-69 0.35497029936059477 2201 70-71 0.5576596084307823 2201 72-73 0.12400590419601087 2201 74-75 0.3986329155852921 2201 76-77 0.38132410653330595 2201 78-79 0.6675668750628923 2201 80-81 0.6889782225164574 2201 82-83 0.6313691713330449 2201 84-85 0.6707594632352105 2201 86-87 0.6322561440627723 2201 88-89 0.6231213602441414 2201 90-91 0.8117015369232767 2201 92-93 0.6134461971766569 2201 94-95 0.4864791764100822 2201 96-97 0.6120231979696378 2201 98-99 0.5338282193466277 2201 100-101 0.5380698942963882 1101 1 0.1872430633857718 1101 2 0.16391526396178335 1101 3 0.14429208312002118 1101 4 0.10030943336933973 1101 5 0.19371352831581845 1101 6 0.08697053165187896 1101 7 0.17789395957736787 1101 8 0.14738332043852864 1101 9 0.1422143876419355 1101 10-11 0.18649287441022722 1101 12-13 0.20437010429960623 1101 14-15 0.35338651408904553 1101 16-17 0.3363099621068244 1101 18-19 0.3130651531535662 1101 20-21 0.37363310216105816 1101 22-23 0.31708523960575263 1101 24-25 0.2853880230969068 1101 26-27 0.3069273151231613 1101 28-29 0.36000826206801406 1101 30-31 0.43739741566404433 1101 32-33 0.43897579260355 1101 34-35 0.32081840836472253 1101 36-37 0.37770961975798656 1101 38-39 0.4069925002742494 1101 40-41 0.5694718128494003 1101 42-43 0.45926206014009807 1101 44-45 0.5130511292257367 1101 46-47 0.6474372843964886 1101 48-49 0.5680069981307057 1101 50-51 0.4907043290036057 1101 52-53 0.4936263431072021 1101 54-55 0.7054043700098731 1101 56-57 0.5515859557109479 1101 58-59 0.6125234820929677 1101 60-61 0.5387983719912057 1101 62-63 0.49750160157483947 1101 64-65 0.5036087548409753 1101 66-67 0.4189453008828039 1101 68-69 0.48949097388126717 1101 70-71 0.35119328196445565 1101 72-73 0.02467580486590748 1101 74-75 0.20904262599500356 1101 76-77 0.06300578821498704 1101 78-79 0.415855583351604 1101 80-81 0.455352568890806 1101 82-83 0.2889151088789781 1101 84-85 0.26127711375285756 1101 86-87 0.24014165194828152 1101 88-89 0.33998983711261843 1101 90-91 0.40812565334739404 1101 92-93 0.36316878689924437 1101 94-95 0.1751005250314286 1101 96-97 0.17167795762439653 1101 98-99 0.3036948692132775 1101 100-101 0.40370419993069007 2304 1 0.17906355719521372 2304 2 0.1510105259741792 2304 3 0.08570207889465564 2304 4 0.08663567925359672 2304 5 0.19710163192491592 2304 6 0.1251381589907723 2304 7 0.1758584505805345 2304 8 0.16687579849454437 2304 9 0.11998626585580752 2304 10-11 0.17064122452541852 2304 12-13 0.18118457895938178 2304 14-15 0.3023709121342151 2304 16-17 0.33099182413674555 2304 18-19 0.3220497903702437 2304 20-21 0.27584312663626775 2304 22-23 0.3925310951621057 2304 24-25 0.34542477578558817 2304 26-27 0.362671337828516 2304 28-29 0.4255961057774087 2304 30-31 0.3143499720088627 2304 32-33 0.32833744384365815 2304 34-35 0.43468708726103245 2304 36-37 0.41614623532167627 2304 38-39 0.42384189397447614 2304 40-41 0.43989068188705716 2304 42-43 0.5512898359247842 2304 44-45 0.47775220470028756 2304 46-47 0.4285429742867066 2304 48-49 0.5248209944474667 2304 50-51 0.40451983210087405 2304 52-53 0.42902835055341626 2304 54-55 0.6364032299590079 2304 56-57 0.5504392080448639 2304 58-59 0.5061282750900276 2304 60-61 0.4902883307739856 2304 62-63 0.4500714994486117 2304 64-65 0.4754574708885926 2304 66-67 0.5643236303495271 2304 68-69 0.3255227199130175 2304 70-71 0.4737406977162877 2304 72-73 0.1665551348667833 2304 74-75 0.39703781067526833 2304 76-77 0.49824212952867697 2304 78-79 0.6639073719558759 2304 80-81 0.922604727413681 2304 82-83 0.875421371186345 2304 84-85 0.9235727616286162 2304 86-87 0.7170635943398374 2304 88-89 0.855217329080439 2304 90-91 0.8467523305376048 2304 92-93 0.5975012193532265 2304 94-95 0.6514080358140752 2304 96-97 0.7043153018639501 2304 98-99 0.495036780831434 2304 100-101 0.770668794574842 2202 1 0.10601173873239134 2202 2 0.15390950292940886 2202 3 0.09021980136713381 2202 4 0.04011970336971871 2202 5 0.12467792513572817 2202 6 0.061134989922798866 2202 7 0.13474459521127358 2202 8 0.2134854059322464 2202 9 0.1397233172421224 2202 10-11 0.09901428624721831 2202 12-13 0.1452054539942722 2202 14-15 0.09676862416316823 2202 16-17 0.12815886943101162 2202 18-19 0.1076317314843962 2202 20-21 0.1836223482339463 2202 22-23 0.14876899733513227 2202 24-25 0.1916852155537967 2202 26-27 0.05386667065567963 2202 28-29 0.1491552452073961 2202 30-31 0.22295297121959834 2202 32-33 0.16770606106766905 2202 34-35 0.24958170674779723 2202 36-37 0.1742215066881272 2202 38-39 0.17433435993550006 2202 40-41 0.1777235315573904 2202 42-43 0.2574849736862106 2202 44-45 0.19105358726621802 2202 46-47 0.16258764734153175 2202 48-49 0.3135076318442671 2202 50-51 0.32451121919832815 2202 52-53 0.3446468139755936 2202 54-55 0.35511708652867213 2202 56-57 0.4016316599011418 2202 58-59 0.3201902639802867 2202 60-61 0.5074760230262712 2202 62-63 0.2884913469372137 2202 64-65 0.2803956350499206 2202 66-67 0.13939542954584994 2202 68-69 0.32978670687662515 2202 70-71 0.24103312526437293 2202 72-73 -0.12403564422576707 2202 74-75 -0.10185916760640978 2202 76-77 -0.1493305024483007 2202 78-79 0.35750861557497515 2202 80-81 0.4579473378734029 2202 82-83 0.4593479544449046 2202 84-85 0.3929866040935259 2202 86-87 0.2261385025839111 2202 88-89 0.5452350650574687 2202 90-91 0.5492283149443509 2202 92-93 0.3813536776316653 2202 94-95 0.3745482340608852 2202 96-97 0.26695391107525523 2202 98-99 0.11975701154918639 2202 100-101 0.29733172998407653 1102 1 0.11683119393134689 1102 2 0.14190457961067438 1102 3 0.07971961067520539 1102 4 0.10645968973236108 1102 5 0.16059388200468305 1102 6 0.13400270751383658 1102 7 0.1339832721560441 1102 8 0.07590869577241222 1102 9 0.12675800005789029 1102 10-11 0.07379975783413073 1102 12-13 0.16194517107680184 1102 14-15 0.2705987581098057 1102 16-17 0.2033553398968735 1102 18-19 0.215365589124211 1102 20-21 0.18754294527302307 1102 22-23 0.17860852719287124 1102 24-25 0.2053940867731754 1102 26-27 0.22198572597945088 1102 28-29 0.302021402591798 1102 30-31 0.2838572265387498 1102 32-33 0.2311674661782135 1102 34-35 0.18487285864258496 1102 36-37 0.22281385358562744 1102 38-39 0.21896334857488142 1102 40-41 0.2533604714721065 1102 42-43 0.32712465263035284 1102 44-45 0.2734173292195905 1102 46-47 0.2564252645014946 1102 48-49 0.30548166885005656 1102 50-51 0.30033567283707185 1102 52-53 0.2467268005693697 1102 54-55 0.43234543721690244 1102 56-57 0.4053433626066507 1102 58-59 0.3458862894983312 1102 60-61 0.38887604845186274 1102 62-63 0.38847516701649454 1102 64-65 0.41440900659868163 1102 66-67 0.4160959103206423 1102 68-69 0.3350212001561772 1102 70-71 0.34540415973916794 1102 72-73 0.3298345350246379 1102 74-75 0.25202488014747004 1102 76-77 0.0032075781508211776 1102 78-79 0.494560489556509 1102 80-81 0.49080066689209545 1102 82-83 0.3825184676951032 1102 84-85 0.3341988846533539 1102 86-87 0.2667642610176948 1102 88-89 0.4670349078810929 1102 90-91 0.5775937384537748 1102 92-93 0.35576542194269223 1102 94-95 0.30612306041566484 1102 96-97 0.10452773196353604 1102 98-99 0.06110422109071578 1102 100-101 0.11619621736951657 2305 1 0.11846725869159513 2305 2 0.15025548836323566 2305 3 0.09048197033031613 2305 4 0.07677790820515895 2305 5 0.11274480316341595 2305 6 0.06474521728370775 2305 7 0.12446153032860963 2305 8 0.06814708048800355 2305 9 0.017599138945058712 2305 10-11 0.0540855651661829 2305 12-13 0.01377663314490718 2305 14-15 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-1.6278071996683074 1213 100-101 -1.4729617061177436 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 12.0 10 22.0 11 29.0 12 31.0 13 97.0 14 277.0 15 774.0 16 1645.0 17 2617.0 18 3459.0 19 4593.0 20 6110.0 21 8563.0 22 11775.0 23 16370.0 24 22057.0 25 30182.0 26 41935.0 27 65064.0 28 116156.0 29 180595.0 30 123941.0 31 68443.0 32 84511.0 33 119665.0 34 185779.0 35 326586.0 36 589560.0 37 728871.0 38 112459.0 39 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.90885395537882 19.832055025371226 19.914418871260565 12.34467214798939 2 29.236101128598524 30.764144295125533 28.75468496358964 11.245069612686303 3 37.33021060861578 14.225635559793984 34.582376472980606 13.86177735860964 4 29.47670669648946 13.781343828115219 28.451621114904608 28.290328360490708 5 43.67406327023606 12.405643343232091 29.316810473282125 14.603482913249726 6 38.21966124513973 13.192226378843072 33.68959508591583 14.89851729010136 7 32.156187657991524 28.115601586138467 23.29341107421964 16.434799681650368 8 27.886936796378947 37.42364692641846 18.79893135498438 15.89048492221821 9 42.476430557357276 21.433695274892102 19.080537548076755 17.009336619673867 10-11 31.116537039264074 30.685248211085746 23.10487635814258 15.093338391507597 12-13 24.175867134607902 29.00729609671097 22.672787066870953 24.144049701810175 14-15 23.36181417216825 29.426059091020644 22.859046143166175 24.353080593644926 16-17 29.40639527158149 23.12251135175957 23.87003931349859 23.601054063160344 18-19 21.091837557543098 23.289116159048596 31.138029808464314 24.48101647494399 20-21 27.302021940880934 24.17726955588824 31.19822874192293 17.3224797613079 22-23 20.313493746954116 37.693893507140075 24.242780159946147 17.74983258595966 24-25 20.777046570904666 22.244224653864897 32.1799762289593 24.79875254627114 26-27 19.32627681692442 22.33822194017972 25.55688396015721 32.77861728273865 28-29 25.892728796266756 30.572187882378927 23.895330287741785 19.639753033612532 30-31 17.39917467507652 24.696147899348226 31.150230873603274 26.754446551971977 32-33 17.940141153701866 30.573835727383326 24.18596456782635 27.30005855108845 34-35 19.038044183282437 30.19372696961304 31.452733143772722 19.315495703331802 36-37 17.813660284481156 32.19834794773176 32.011475312126386 17.9765164556607 38-39 17.76397652723886 25.231202209415542 30.02206750890642 26.98275375443918 40-41 26.09302998295078 24.166337223826826 22.8039778579349 26.93665493528749 42-43 19.607978226856172 31.290654815905615 30.246157487147794 18.855209470090415 44-45 18.10618544411643 25.352502296489 29.947636544678108 26.593675714716465 46-47 25.91415390306353 24.501700109308395 23.92582807463826 25.658317912989816 48-49 18.856642051573708 30.812017560453253 31.40264304421863 18.92869734375441 50-51 25.13755449366886 24.020989803304744 29.638595235745218 21.202860467281184 52-53 17.543431821747863 24.69073317140175 23.393813699201708 34.37202130764868 54-55 25.526289627660553 24.63839367980796 31.17757901423562 18.65773767829587 56-57 19.024164440583075 24.472319560107763 29.916862681838225 26.586653317470937 58-59 18.08481183368412 24.16067805419236 30.204683330799536 27.549826781323983 60-61 24.33499772400012 23.79315895456908 31.717320814983303 20.1545225064475 62-63 17.770830809451937 23.58899178045975 31.6281800686449 27.01199734144342 64-65 25.953440274804002 25.198630468430572 30.201684703367516 18.646244553397914 66-67 19.50150355505291 38.132873938086846 23.21394317175596 19.151679335104284 68-69 18.636534043425456 36.68794698577326 24.867182121726373 19.808336849074912 70-71 18.15927111110176 38.88530878686796 23.75205096007815 19.203369141952127 72-73 20.014935550766406 38.42136243153151 23.30898021469109 18.25472180301099 74-75 21.326416754944695 36.08591268550442 23.50841112350501 19.079259436045877 76-77 20.715321424870226 34.28495124920675 24.01206499497876 20.987662330944264 78-79 20.5040504776376 34.27427174337285 24.545515754273247 20.676162024716312 80-81 19.77077454319007 34.4975125808569 24.688213546371045 21.043499329581987 82-83 19.382607727658797 33.602983004970795 24.918732573499184 22.09567669387122 84-85 20.195799679277307 32.58333241292547 24.806061297761058 22.414806610036162 86-87 19.002893974347014 33.037126478462476 25.579636548805833 22.380342998384677 88-89 18.691863273694413 33.313934332951725 25.663404606979118 22.33079778637475 90-91 19.521134850858303 32.04106652471212 24.949546169769643 23.488252454659936 92-93 19.99235708646103 32.089942255490776 24.21118222438598 23.70651843366221 94-95 20.395250289990752 33.120328535756286 23.71545584932477 22.768965324928192 96-97 19.9479004549723 33.802872226348065 22.98861297894186 23.260614339737785 98-99 19.944561271702554 32.03228044995652 24.620919368848703 23.402238909492226 100-101 20.850704653160808 32.98460041594713 23.875554679475364 22.289140251416697 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1293.0 1 756.5 2 341.0 3 560.0 4 505.5 5 324.5 6 311.5 7 333.0 8 350.0 9 370.0 10 360.0 11 337.0 12 317.5 13 287.0 14 279.5 15 295.5 16 296.5 17 313.5 18 341.5 19 354.0 20 373.0 21 425.5 22 479.5 23 519.0 24 579.5 25 696.0 26 876.5 27 1159.0 28 1490.5 29 1948.0 30 2528.0 31 3529.0 32 5900.0 33 10346.5 34 18198.5 35 32053.0 36 52450.5 37 80389.0 38 117559.5 39 149680.5 40 168166.0 41 171505.5 42 187812.5 43 218815.0 44 209905.0 45 180414.0 46 174235.5 47 168832.5 48 146284.0 49 121270.5 50 98977.0 51 85734.0 52 81574.0 53 82594.5 54 73938.0 55 47993.0 56 28910.0 57 23509.5 58 22481.5 59 19887.5 60 15334.0 61 11325.0 62 7626.5 63 4792.0 64 3267.5 65 2222.0 66 1520.5 67 1158.5 68 894.5 69 669.0 70 501.0 71 362.0 72 254.5 73 190.0 74 133.5 75 82.5 76 58.0 77 36.5 78 19.0 79 18.0 80 16.5 81 8.5 82 5.5 83 3.5 84 2.5 85 4.0 86 2.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17835292632730407 2 0.0 3 0.0 4 0.008870314598153713 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0021386924525192744 12-13 0.0 14-15 0.0 16-17 3.155447880766143E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.001858208196451173 40-41 0.006047941771468441 42-43 0.0025418885706171702 44-45 0.0010518159602553809 46-47 0.0065738497515961305 48-49 0.008186634223987715 50-51 0.0055570943233492626 52-53 0.006398547091553567 54-55 0.01838924903846491 56-57 0.0211239705351289 58-59 0.026523292464439855 60-61 0.02510334091809509 62-63 0.010044842420438887 64-65 0.015146149827677485 66-67 0.022263437825405564 68-69 0.026260338474376012 70-71 0.028101016404822925 72-73 0.02189530223931618 74-75 0.031431766945631635 76-77 0.02876716651298467 78-79 0.02001956377686075 80-81 0.009045617258196276 82-83 0.0018406779304469165 84-85 0.00748542358381746 86-87 0.026926488582537753 88-89 0.024139176287860994 90-91 0.02079089548104803 92-93 0.04217782000624078 94-95 0.04407108873470046 96-97 0.032869248757980654 98-99 0.03977617356365765 100-101 0.03949568930758955 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2852210.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.063974558740558 #Duplication Level Percentage of deduplicated Percentage of total 1 75.63446452694158 18.20065830140288 2 9.665193179974024 4.651659255764153 3 3.805094439307328 2.7469708734329017 4 2.278831946334476 2.1935101592095227 5 1.5402646242615166 1.8532444365978613 6 1.1545881644690086 1.6670388129363107 7 0.8067076850634644 1.358881524679539 8 0.6577644108376771 1.2662740838434268 9 0.4695081747014748 1.0168409494023307 >10 3.210952148208347 15.432557681118848 >50 0.4000602840757129 6.757019757913264 >100 0.329145711211294 16.253709605777168 >500 0.033452020017358974 5.259036934858292 >1k 0.012507165705740635 5.362667064256299 >5k 7.327594455481686E-4 1.2172484805129744 >10k+ 7.327594454177112E-4 14.762682078294208 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 356811 12.509983486489423 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 20556 0.720704295966987 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 19126 0.6705677351948138 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11181 0.39201180838718047 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 10933 0.3833167964490693 TruSeq Adapter, Index 27 (97% over 39bp) TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 8557 0.30001297239684316 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 7722 0.27073742816973506 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7702 0.2700362175295648 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5450 0.1910798994463942 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 5085 0.17828280526328708 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 4975 0.17442614674235069 No Hit GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA 4197 0.1471490528397278 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 3823 0.13403641386854404 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 3770 0.13217820567209285 No Hit GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA 3690 0.12937336311141184 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG 3580 0.12551670459047545 TruSeq Adapter, Index 27 (97% over 38bp) GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 3517 0.12330789107393915 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3488 0.12229113564569229 No Hit CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC 3096 0.10854740709835531 TruSeq Adapter, Index 27 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.006591380017600387 0.0 0.0 2 0.007257530125762128 0.0 0.0 3 0.008028861829949408 0.0 0.0 4 0.008484648746060072 0.0 0.0 5 0.010342856942511245 0.0 0.0 6 0.011359612370758113 0.0 0.0 7 0.013323002163234825 0.0 0.0 8 0.014970847167634922 0.0 0.0 9 0.016934236960111634 0.0 0.0 10-11 0.026190217410358985 0.0 0.0 12-13 0.03346527780212537 0.0 0.0 14-15 0.040565035533849186 0.0 0.0 16-17 0.047875156457624087 0.0 0.0 18-19 0.055097626051377704 0.0 0.0 20-21 0.0604794177146844 0.0 0.0 22-23 0.06552813432391023 0.0 0.0 24-25 0.07120794050928929 0.0 0.0 26-27 0.07748377573881307 0.0 0.0 28-29 0.08491660852461774 0.0 0.0 30-31 0.1665725875724438 0.0 0.0 32-33 0.17786207887918493 0.0 0.0 34-35 0.18771408837357698 0.0 0.0 36-37 0.19942430606442021 0.0 0.0 38-39 0.21178314359742095 0.0 0.0 40-41 0.2260177195928771 0.0 0.0 42-43 0.24198779192275463 0.0 0.0 44-45 0.25893955914887057 0.0 0.0 46-47 0.2773813989853482 0.0 0.0 48-49 0.2984177181904558 0.0 0.0 50-51 0.3207512770798784 0.0 0.0 52-53 0.34415418219556065 0.0 0.0 54-55 0.37153645769420907 0.0 0.0 56-57 0.4006191689952703 0.0 0.0 58-59 0.4333657058912212 0.0 0.0 60-61 0.46669074156531254 0.0 0.0 62-63 0.5059410071488425 0.0 0.0 64-65 0.5465586334807044 0.0 0.0 66-67 0.5906472524814091 0.0 0.0 68-69 0.6386100602690545 0.0 0.0 70-71 0.6946718509506664 0.0 0.0 72-73 0.7537488473850102 0.0 0.0 74-75 0.8176466669705246 0.0 0.0 76-77 0.8893805154599416 0.0 0.0 78-79 0.9705281167936443 0.0 0.0 80-81 1.0615452578877433 0.0 0.0 82-83 1.1650439483768726 0.0 0.0 84-85 1.2847055441219264 0.0 0.0 86-87 1.4184439434683982 0.0 0.0 88-89 1.5652073304560323 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 46850 0.0 82.60715 3 CGGAAGA 46895 0.0 82.335464 4 GATCGGA 47055 0.0 82.01327 1 GAAGAGC 47175 0.0 81.96471 6 ATCGGAA 47680 0.0 80.7508 2 GGAAGAG 49130 0.0 78.7128 5 GAGCACA 55210 0.0 69.97574 9 AGAGCAC 55280 0.0 69.86136 8 AAGAGCA 55485 0.0 69.569 7 GTACCCC 70 1.8189894E-11 61.173027 1 CTCGGAA 280 0.0 61.061485 2 GCGCACA 160 0.0 59.363255 9 ATCGGAC 185 0.0 59.04444 2 CGCGTCG 25 0.004989104 57.094826 1 GACGCAT 50 4.2500324E-7 56.98872 7 CGGCAGA 175 0.0 51.563034 4 TTGTGTT 24230 0.0 51.545574 1 CGTGGCA 150 0.0 47.57902 1 CGAGGGG 290 0.0 45.938366 1 TTCCCTA 610 0.0 45.933537 8 >>END_MODULE