FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_50330665-1.341000000356c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_50330665-1.341000000356c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2852210
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT35681112.509983486489423TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC205560.720704295966987No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA191260.6705677351948138No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC111810.39201180838718047No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT109330.3833167964490693TruSeq Adapter, Index 27 (97% over 39bp)
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT85570.30001297239684316No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT77220.27073742816973506No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC77020.2700362175295648No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT54500.1910798994463942No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT50850.17828280526328708No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC49750.17442614674235069No Hit
GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA41970.1471490528397278No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT38230.13403641386854404No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC37700.13217820567209285No Hit
GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA36900.12937336311141184No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG35800.12551670459047545TruSeq Adapter, Index 27 (97% over 38bp)
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC35170.12330789107393915No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34880.12229113564569229No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC30960.10854740709835531TruSeq Adapter, Index 27 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG468500.082.607153
CGGAAGA468950.082.3354644
GATCGGA470550.082.013271
GAAGAGC471750.081.964716
ATCGGAA476800.080.75082
GGAAGAG491300.078.71285
GAGCACA552100.069.975749
AGAGCAC552800.069.861368
AAGAGCA554850.069.5697
GTACCCC701.8189894E-1161.1730271
CTCGGAA2800.061.0614852
GCGCACA1600.059.3632559
ATCGGAC1850.059.044442
CGCGTCG250.00498910457.0948261
GACGCAT504.2500324E-756.988727
CGGCAGA1750.051.5630344
TTGTGTT242300.051.5455741
CGTGGCA1500.047.579021
CGAGGGG2900.045.9383661
TTCCCTA6100.045.9335378
Produced by FastQC (version 0.11.2)