##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_50220371-2.34100000035905.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3410268 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65283256330588 33.0 33.0 33.0 33.0 33.0 2 32.679287082422846 33.0 33.0 33.0 33.0 33.0 3 32.73376315292523 33.0 33.0 33.0 33.0 33.0 4 36.681614172258605 37.0 37.0 37.0 37.0 37.0 5 36.627381777619824 37.0 37.0 37.0 37.0 37.0 6 36.52319964296061 37.0 37.0 37.0 37.0 37.0 7 36.49220882347077 37.0 37.0 37.0 37.0 37.0 8 36.42924515023453 37.0 37.0 37.0 37.0 37.0 9 36.41477326708634 37.0 37.0 37.0 37.0 37.0 10-11 36.37424419429793 37.0 37.0 37.0 37.0 37.0 12-13 36.37444945675824 37.0 37.0 37.0 37.0 37.0 14-15 38.259990270559385 40.0 37.0 40.0 37.0 40.0 16-17 38.11945366170635 40.0 37.0 40.0 37.0 40.0 18-19 37.91834395419949 40.0 37.0 40.0 35.0 40.0 20-21 37.807816130579766 40.0 37.0 40.0 33.0 40.0 22-23 37.66257006780699 40.0 37.0 40.0 33.0 40.0 24-25 37.57768641643413 40.0 37.0 40.0 33.0 40.0 26-27 37.45693520274653 40.0 37.0 40.0 33.0 40.0 28-29 37.3108353947549 40.0 37.0 40.0 33.0 40.0 30-31 37.231095913869524 40.0 37.0 40.0 33.0 40.0 32-33 37.08759458200939 40.0 37.0 40.0 33.0 40.0 34-35 36.984204027366765 37.0 37.0 40.0 33.0 40.0 36-37 36.88171369522864 37.0 37.0 40.0 33.0 40.0 38-39 36.75628968163206 37.0 37.0 40.0 33.0 40.0 40-41 36.6293726768688 37.0 37.0 40.0 33.0 40.0 42-43 36.492239466223765 37.0 37.0 40.0 33.0 40.0 44-45 36.335852050337394 37.0 37.0 40.0 33.0 40.0 46-47 36.2314586419601 37.0 37.0 40.0 33.0 40.0 48-49 36.01652494759942 37.0 37.0 40.0 33.0 40.0 50-51 35.841717571756824 37.0 37.0 40.0 27.0 40.0 52-53 35.67619817562725 37.0 37.0 40.0 27.0 40.0 54-55 35.39561333596069 37.0 37.0 40.0 27.0 40.0 56-57 35.21308779837831 37.0 33.0 40.0 27.0 40.0 58-59 34.98772999658678 37.0 33.0 40.0 27.0 40.0 60-61 34.719288044224086 37.0 33.0 40.0 27.0 40.0 62-63 34.37107728776741 37.0 33.0 37.0 27.0 40.0 64-65 34.13001661453001 37.0 33.0 37.0 27.0 40.0 66-67 33.8370245388339 37.0 33.0 37.0 27.0 40.0 68-69 33.561313216439295 37.0 33.0 37.0 24.5 40.0 70-71 33.17196683662398 37.0 33.0 37.0 22.0 40.0 72-73 32.95196697151074 37.0 33.0 37.0 22.0 37.0 74-75 32.60543306273877 37.0 33.0 37.0 22.0 37.0 76-77 29.858011305856316 33.0 30.0 35.0 18.5 37.0 78-79 32.18623580316854 35.0 33.0 37.0 22.0 37.0 80-81 32.52515667390364 37.0 33.0 37.0 22.0 37.0 82-83 32.46333748549968 37.0 33.0 37.0 22.0 37.0 84-85 32.282551107420296 37.0 33.0 37.0 22.0 37.0 86-87 32.08873848037749 37.0 33.0 37.0 22.0 37.0 88-89 31.835852930033653 33.0 33.0 37.0 15.0 37.0 90-91 31.53448380010017 33.0 33.0 37.0 15.0 37.0 92-93 31.487498489854758 33.0 33.0 37.0 15.0 37.0 94-95 31.38162293989798 33.0 33.0 37.0 15.0 37.0 96-97 31.173197238457504 33.0 33.0 37.0 15.0 37.0 98-99 30.951275237019495 33.0 33.0 37.0 15.0 37.0 100-101 29.422097324902325 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.15887254697032915 2306 2 0.16793305417643722 2306 3 0.1381380642168395 2306 4 0.1268421896251155 2306 5 0.15809781557539537 2306 6 0.10398185012770256 2306 7 0.15009091838796706 2306 8 0.12398452607670407 2306 9 0.16624366214026054 2306 10-11 0.0708313331393029 2306 12-13 0.09672441893561512 2306 14-15 0.2722414864116871 2306 16-17 0.3671581073758645 2306 18-19 0.36223990064623024 2306 20-21 0.3403447490248297 2306 22-23 0.46962145905092 2306 24-25 0.3574708920545433 2306 26-27 0.28293269679127064 2306 28-29 0.3604681537082186 2306 30-31 0.39395843044460577 2306 32-33 0.3276576709175174 2306 34-35 0.22632113079758653 2306 36-37 0.31392787332743666 2306 38-39 0.29506032097399526 2306 40-41 0.2857380063108863 2306 42-43 0.17971477565264848 2306 44-45 0.2587423026436966 2306 46-47 0.2103465710353376 2306 48-49 0.3271031987009323 2306 50-51 0.32823004420213664 2306 52-53 0.19489661945736003 2306 54-55 0.14271847554179828 2306 56-57 0.24291830699132788 2306 58-59 0.17839130258855818 2306 60-61 0.18598162072962054 2306 62-63 0.11051174079342019 2306 64-65 0.11990071387150181 2306 66-67 0.13904425281680943 2306 68-69 0.366459881395393 2306 70-71 0.38502527458341973 2306 72-73 0.12958468804488632 2306 74-75 0.05926671480668233 2306 76-77 -0.20323933690425378 2306 78-79 0.2174605596110908 2306 80-81 0.34177232620164943 2306 82-83 0.4074687056366386 2306 84-85 0.47183949385237156 2306 86-87 0.28034337993499747 2306 88-89 0.564410647525289 2306 90-91 0.5392397061557581 2306 92-93 0.4689863104275389 2306 94-95 0.38040330049998516 2306 96-97 0.49951083933243723 2306 98-99 0.3784542993730504 2306 100-101 0.32762187407965015 2307 1 0.13028112500673927 2307 2 0.15474975694954196 2307 3 0.11259675982151407 2307 4 0.10309782220435437 2307 5 0.17688129534181485 2307 6 0.254892540428969 2307 7 0.13537798872513918 2307 8 0.149149774657225 2307 9 0.22653099927378406 2307 10-11 0.17045614123649244 2307 12-13 0.13734098785317173 2307 14-15 0.17589776529874257 2307 16-17 0.30410803046228807 2307 18-19 0.4362570863969637 2307 20-21 0.4437519671621004 2307 22-23 0.45774495578050534 2307 24-25 0.5559554379391969 2307 26-27 0.48499231383432573 2307 28-29 0.4585963612991648 2307 30-31 0.5174188249068195 2307 32-33 0.5222792596720325 2307 34-35 0.49936560244678674 2307 36-37 0.4441824275412216 2307 38-39 0.493721460636543 2307 40-41 0.503133077845348 2307 42-43 0.5510721796072389 2307 44-45 0.48249881565091357 2307 46-47 0.5131502704081896 2307 48-49 0.6510224320755711 2307 50-51 0.339329026666249 2307 52-53 0.3715788212946549 2307 54-55 0.599179089031125 2307 56-57 0.5367256332700592 2307 58-59 0.4987026869668938 2307 60-61 0.15895692859990618 2307 62-63 0.2584513360658889 2307 64-65 0.230601127990262 2307 66-67 0.3186459690542378 2307 68-69 0.3290054845152355 2307 70-71 0.4767653610660929 2307 72-73 0.323253178582263 2307 74-75 0.36296812039151405 2307 76-77 0.10396218276778413 2307 78-79 0.4510743086182245 2307 80-81 0.6547180984449739 2307 82-83 0.5197808238320007 2307 84-85 0.5325150832055243 2307 86-87 0.45963383375813294 2307 88-89 0.6451546587093908 2307 90-91 0.7881843360024909 2307 92-93 0.6755570726977567 2307 94-95 0.7540635381367728 2307 96-97 0.6948898756436712 2307 98-99 0.6732906973071842 2307 100-101 0.640031766786862 2201 1 0.1762216971927515 2201 2 0.12935806198734667 2201 3 0.09186490217803822 2201 4 0.09713278859391039 2201 5 0.14354588086635545 2201 6 0.08666245575850695 2201 7 0.06303636764349108 2201 8 0.11025434884754048 2201 9 0.06987734774983068 2201 10-11 0.1293361345768389 2201 12-13 0.1339659356245093 2201 14-15 0.22653459479077043 2201 16-17 0.3984963107505095 2201 18-19 0.4442482719596015 2201 20-21 0.49703080658917287 2201 22-23 0.500236595192213 2201 24-25 0.3977344462589656 2201 26-27 0.5192129341107332 2201 28-29 0.5333966917204975 2201 30-31 0.5659813160019382 2201 32-33 0.4553579732700541 2201 34-35 0.45652993423206567 2201 36-37 0.4722343024101434 2201 38-39 0.4244801810222967 2201 40-41 0.5799334658983142 2201 42-43 0.6192050909489808 2201 44-45 0.5119536204477839 2201 46-47 0.6797673898833594 2201 48-49 0.8470290405022851 2201 50-51 0.7858745708608978 2201 52-53 0.687349956493648 2201 54-55 0.8271776546439327 2201 56-57 0.8580395703587271 2201 58-59 0.7196372958802684 2201 60-61 0.6929562274150953 2201 62-63 0.7282275492516845 2201 64-65 0.519900135285468 2201 66-67 0.61048284913403 2201 68-69 0.6101889179835212 2201 70-71 0.6559377708845986 2201 72-73 0.7110774565460076 2201 74-75 0.7029025163880362 2201 76-77 0.6902125569731474 2201 78-79 0.9121936909352399 2201 80-81 0.8980136990705958 2201 82-83 0.8856308023001773 2201 84-85 0.9185574232417366 2201 86-87 0.8837100067618806 2201 88-89 1.114259223294436 2201 90-91 1.3004642752331428 2201 92-93 1.1091491080067328 2201 94-95 1.1276913850991797 2201 96-97 1.0616413088302075 2201 98-99 1.071898093045963 2201 100-101 1.233384838952869 1101 1 0.10632270729376359 1101 2 0.14547429679923596 1101 3 0.10162335628431407 1101 4 0.04980004082638345 1101 5 0.10990366157196263 1101 6 0.03410499711409187 1101 7 0.1259085679069969 1101 8 0.08520530771154 1101 9 0.020039842260153762 1101 10-11 0.06706047947509575 1101 12-13 0.05326398861821957 1101 14-15 0.11512777294916532 1101 16-17 0.19623822262285984 1101 18-19 0.3602539812261796 1101 20-21 0.3277547123130873 1101 22-23 0.41459613259522854 1101 24-25 0.31223598336919167 1101 26-27 0.5161189507993598 1101 28-29 0.45194522048641517 1101 30-31 0.466555902446089 1101 32-33 0.4356866220769575 1101 34-35 0.292462447773282 1101 36-37 0.44312509438789505 1101 38-39 0.4391332332840463 1101 40-41 0.3707891239278851 1101 42-43 0.41061630453410913 1101 44-45 0.5653996751981865 1101 46-47 0.6074705080212581 1101 48-49 0.6467033195243843 1101 50-51 0.506193704223513 1101 52-53 0.6522687092384913 1101 54-55 0.7800849888556058 1101 56-57 0.6056570494979496 1101 58-59 0.5673931149404368 1101 60-61 0.5619849201394373 1101 62-63 0.45694442818595604 1101 64-65 0.5393079818672248 1101 66-67 0.2570353304691153 1101 68-69 0.4236049624865288 1101 70-71 0.49056154485619885 1101 72-73 0.4961623635874304 1101 74-75 0.6693364206480297 1101 76-77 0.46109822525012234 1101 78-79 0.7216359395079266 1101 80-81 0.728122760701396 1101 82-83 0.7397729483988442 1101 84-85 0.7882814753570955 1101 86-87 0.7335672750974069 1101 88-89 0.884072574194743 1101 90-91 0.9886109594667865 1101 92-93 0.8261927326590524 1101 94-95 0.7215487998261629 1101 96-97 0.8954760328823248 1101 98-99 0.7941232430972001 1101 100-101 0.9013543895311109 2304 1 0.12873595841212904 2304 2 0.14752281975091108 2304 3 0.072828772799852 2304 4 0.06473132684580918 2304 5 0.09928140710857036 2304 6 0.08892948427807568 2304 7 0.04797603254247207 2304 8 0.10468017975442478 2304 9 0.13724936952408484 2304 10-11 0.08489044763694409 2304 12-13 0.11291316445346666 2304 14-15 0.11932355741699041 2304 16-17 0.21744384064567157 2304 18-19 0.33391073373493896 2304 20-21 0.30139655328354564 2304 22-23 0.348176513847001 2304 24-25 0.3010148492862612 2304 26-27 0.27075381961812894 2304 28-29 0.28008510683631727 2304 30-31 0.32880250704851477 2304 32-33 0.3127379661304843 2304 34-35 0.3645833118364692 2304 36-37 0.28690597537769236 2304 38-39 0.31904557761966856 2304 40-41 0.4175466703753088 2304 42-43 0.37346682643794793 2304 44-45 0.3492460460521727 2304 46-47 0.4445047239564488 2304 48-49 0.49864282704060514 2304 50-51 0.5736502794058822 2304 52-53 0.5193694225742362 2304 54-55 0.5912432526689315 2304 56-57 0.6089244899525497 2304 58-59 0.422867664571676 2304 60-61 0.28979675264659477 2304 62-63 0.46083775023488727 2304 64-65 0.3007404970810299 2304 66-67 0.4422064101450971 2304 68-69 0.31718756031830253 2304 70-71 0.46751132924341476 2304 72-73 0.3361698193565843 2304 74-75 0.3976268329319126 2304 76-77 0.4910720293044477 2304 78-79 0.6085722229077604 2304 80-81 0.6701239294435837 2304 82-83 0.6443961297957017 2304 84-85 0.7204176539731435 2304 86-87 0.7089239429731222 2304 88-89 0.8468044083139539 2304 90-91 0.8553525868395333 2304 92-93 0.5935898631954686 2304 94-95 0.7585064429970849 2304 96-97 0.8175360417486424 2304 98-99 0.7516027901088513 2304 100-101 0.6843290399159905 2202 1 0.13350976777196166 2202 2 0.1294311650598985 2202 3 0.10716484714226482 2202 4 0.01772784204532485 2202 5 0.1029322597904283 2202 6 0.033406450466173965 2202 7 0.0017454174147317758 2202 8 0.15268303860699461 2202 9 0.13823956096619838 2202 10-11 0.08681669138320558 2202 12-13 0.04814209457227747 2202 14-15 0.05746720098479585 2202 16-17 0.14967235295572578 2202 18-19 0.1559150220138008 2202 20-21 0.20309460917196276 2202 22-23 0.297568472023265 2202 24-25 0.36890265003586364 2202 26-27 0.31936789594703896 2202 28-29 0.41990583933311143 2202 30-31 0.4039914392487134 2202 32-33 0.4600771518472371 2202 34-35 0.45446901129882633 2202 36-37 0.41089818393313493 2202 38-39 0.3119602375034489 2202 40-41 0.40905447788943405 2202 42-43 0.38183524499355315 2202 44-45 0.43437764050526084 2202 46-47 0.4130401282744245 2202 48-49 0.5700741587180076 2202 50-51 0.515769419178973 2202 52-53 0.48527152217800307 2202 54-55 0.5535589691650387 2202 56-57 0.5300944615935919 2202 58-59 0.5624071796380363 2202 60-61 0.4784148310630201 2202 62-63 0.37779768650756296 2202 64-65 0.414390025936612 2202 66-67 0.33287921232565765 2202 68-69 0.3752880379670671 2202 70-71 0.41900973988666834 2202 72-73 0.3408904461003246 2202 74-75 0.4181620183976804 2202 76-77 0.38337436214651177 2202 78-79 0.6741324233335249 2202 80-81 0.7647495773915054 2202 82-83 0.6943627280519209 2202 84-85 0.6731947430551699 2202 86-87 0.6928499755193442 2202 88-89 0.9005353027460821 2202 90-91 0.8460230551067305 2202 92-93 0.9149995774785538 2202 94-95 0.858260004701922 2202 96-97 0.8286452780768805 2202 98-99 0.8201616866039991 2202 100-101 0.7854730018142817 1102 1 0.05982609692951968 1102 2 0.06723269428668743 1102 3 0.056939913793222274 1102 4 -0.024328803633380858 1102 5 0.08883774560428748 1102 6 0.10551638782227712 1102 7 0.08110332833443579 1102 8 0.09575301156844773 1102 9 0.12217933438930118 1102 10-11 0.041842929971089404 1102 12-13 0.12802652261032677 1102 14-15 0.1858210204883335 1102 16-17 0.14144006872956538 1102 18-19 0.2826020221316128 1102 20-21 0.34698850142794413 1102 22-23 0.2839702947615379 1102 24-25 0.38935536748961397 1102 26-27 0.4144189622896519 1102 28-29 0.4131867463970238 1102 30-31 0.4432124193559659 1102 32-33 0.4530589840977228 1102 34-35 0.39779987931029837 1102 36-37 0.3663069473887788 1102 38-39 0.4162922589073901 1102 40-41 0.41585538460427784 1102 42-43 0.4058681334053773 1102 44-45 0.40732927801713004 1102 46-47 0.46415555915304907 1102 48-49 0.5730610888759529 1102 50-51 0.5112647525210363 1102 52-53 0.5003061675861815 1102 54-55 0.41611509341725395 1102 56-57 0.49435347601753676 1102 58-59 0.4377355256034221 1102 60-61 0.3187597870435752 1102 62-63 0.3251417930792826 1102 64-65 0.44485874980166074 1102 66-67 0.43429030001984614 1102 68-69 0.3967845174292606 1102 70-71 0.45159471349536773 1102 72-73 0.4854099410511452 1102 74-75 0.5618068331494612 1102 76-77 0.16019891849765955 1102 78-79 0.7360045480337263 1102 80-81 0.7528603990636427 1102 82-83 0.7761050561011729 1102 84-85 0.7919328982380875 1102 86-87 0.7805367109241317 1102 88-89 0.8835802152380587 1102 90-91 1.0091510031298903 1102 92-93 0.9910602113062623 1102 94-95 0.9344749938588741 1102 96-97 0.7100409338743141 1102 98-99 0.7218462504892891 1102 100-101 0.7741894272575642 2305 1 0.1011179128235824 2305 2 0.14365480026762611 2305 3 0.10481917950661312 2305 4 0.024658731416373314 2305 5 0.10729734247986755 2305 6 0.04880803900442743 2305 7 0.06163766563476969 2305 8 -0.006079100455728792 2305 9 0.03370702367055145 2305 10-11 0.08637280636187938 2305 12-13 0.04946897918124904 2305 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-1.1029882858785953 1213 64-65 -1.0491372962082792 1213 66-67 -1.1410711361260937 1213 68-69 -1.3050380052400428 1213 70-71 -1.2550267604909493 1213 72-73 -1.2731308363649276 1213 74-75 -1.2320176343716227 1213 76-77 -1.08554819609083 1213 78-79 -1.0863669186557061 1213 80-81 -1.2188374631880876 1213 82-83 -1.4127952307127387 1213 84-85 -1.5451941707069778 1213 86-87 -1.7947196130769427 1213 88-89 -1.9061757287706023 1213 90-91 -1.6767766789755179 1213 92-93 -1.881342696660937 1213 94-95 -1.9771745537524943 1213 96-97 -2.237199327365385 1213 98-99 -2.4160280013948814 1213 100-101 -2.169200570573686 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 25.0 9 108.0 10 154.0 11 133.0 12 181.0 13 468.0 14 1518.0 15 3744.0 16 7291.0 17 11090.0 18 13565.0 19 15138.0 20 17096.0 21 19716.0 22 22773.0 23 26246.0 24 30229.0 25 34616.0 26 40323.0 27 47190.0 28 55392.0 29 65414.0 30 77499.0 31 92953.0 32 116974.0 33 155232.0 34 225324.0 35 373790.0 36 725455.0 37 975176.0 38 255399.0 39 54.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.779593222808565 31.543386786145277 20.13918999425403 15.537829996792127 2 30.325769118438785 24.88555151677229 31.200421785032727 13.588257579756194 3 33.39101208468073 22.843717854432555 26.18093358058663 17.58433648030008 4 29.506326600814614 23.084328968579463 29.863074921252903 17.546269509353024 5 29.293680144786276 21.47526235474749 31.084096616453603 18.146960884012632 6 27.482297578958605 22.136911233955807 32.06991356691029 18.310877620175305 7 30.924754300835005 22.115945139795464 26.48211225628015 20.477188303089374 8 27.557540932266907 26.899381514883874 25.886000748328286 19.657076804520933 9 24.878924471625105 24.605720136951113 30.561263806832777 19.954091584591005 10-11 19.905585656597115 30.150555058514488 34.538093432577334 15.405765852311065 12-13 19.91707103371348 29.73773615446059 34.325982591397505 16.019210220428423 14-15 20.17219761027579 29.60811291077417 34.452526898179265 15.767162580770776 16-17 20.055168806019363 29.466429324094157 34.351305420802895 16.12709644908358 18-19 19.310757880430202 29.757724284092085 34.038986677731295 16.892531157746422 20-21 19.366073282217116 29.97669098147125 33.602886928534645 17.054348807776982 22-23 19.26443904115454 29.61397755249734 33.683745676292894 17.437837730055232 24-25 19.216671534319296 29.043259943206813 34.071926311949674 17.668142210524216 26-27 19.292721275864537 29.448594655903875 33.84083010484807 17.41785396338352 28-29 19.110228287043714 29.834282818828317 33.69213211395702 17.363356780170943 30-31 18.432173072614823 30.924402422331614 33.50189779806162 17.141526706991943 32-33 18.22595760802377 31.355541558610646 33.4885557381414 16.929945095224188 34-35 18.58900825389676 30.79382617436518 33.95441355342161 16.66275201831645 36-37 18.69007069239133 30.38167381566493 34.36111179531931 16.567143696624427 38-39 19.021048106438705 30.244652200993023 33.65175175092986 17.082547941638406 40-41 18.82217562454885 30.073592946009605 33.20774835144968 17.896483077991864 42-43 19.072378303443593 29.746823657747516 33.14116807874088 18.039629960068 44-45 18.67678174172455 29.614422632416908 33.37093595185184 18.337859674006705 46-47 18.826126775246706 29.911710169356827 33.23375910808708 18.028403947309386 48-49 18.705300009736195 30.59067331074067 32.75466017743337 17.949366502089763 50-51 19.263747927644268 30.49331706973042 31.81029941187398 18.432635590751328 52-53 19.23647075383722 30.387270455891752 31.810781168438893 18.56547762183213 54-55 19.54596274129904 30.502489173108838 31.78696350209968 18.164584583492445 56-57 19.51591073639061 30.548769319142878 31.683841600809743 18.25147834365677 58-59 19.29275125621876 31.12380798178127 31.55845043424647 18.024990327753496 60-61 19.170116144435898 31.045593235626036 31.374895232424528 18.409395387513538 62-63 18.720082302125107 30.92538129324663 31.777479852270268 18.577056552357995 64-65 18.68670234543729 30.49116868308031 32.09727059750991 18.724858373972488 66-67 18.949731845492554 30.464405183880515 31.72022179758111 18.865641173045816 68-69 19.307342787693983 30.546189808966144 31.62568111668966 18.520786286650214 70-71 18.879322634067876 30.751075177035357 31.842502330454657 18.52709985844211 72-73 18.848056590995547 30.649633210312405 31.642140206314984 18.860169992377063 74-75 19.16676285627249 30.27254829805998 31.221723372578204 19.338965473089324 76-77 19.20330729198749 30.002208733207908 30.890424540228572 19.904059434576034 78-79 18.911236890314186 30.174260053227712 31.089393700311657 19.825109356146438 80-81 18.626978887854982 30.57202296936626 30.705207404368355 20.0957907384104 82-83 18.185014477165257 30.77808092081457 30.542684031836277 20.494220570183902 84-85 18.145609038156298 30.88108094237882 31.041433436339577 19.931876583125305 86-87 18.402468111355397 30.589501228497262 31.300655438085805 19.707375222061533 88-89 18.779760106483764 30.535137824087084 31.120997986124028 19.564104083305125 90-91 18.62487382619684 30.45342498357889 30.6973328951438 20.22436829508047 92-93 18.289641349582762 30.658352337660606 30.65007927617166 20.401927036584976 94-95 18.07734068491909 30.521911150214738 30.640390634649282 20.76035753021689 96-97 18.07925439926172 30.99040938430943 30.36679715911616 20.56353905731269 98-99 18.62982075616581 30.78316555122404 30.071024850410115 20.515988842200038 100-101 18.736883052419678 30.777544196449263 29.6274038814137 20.858168869717357 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 152306.0 1 84496.0 2 14126.5 3 11712.5 4 10414.5 5 8464.5 6 8088.5 7 8430.5 8 8495.0 9 8076.0 10 7440.5 11 6688.0 12 5973.5 13 5478.5 14 5152.0 15 5040.0 16 5084.5 17 5079.5 18 5136.5 19 5275.5 20 5498.0 21 5764.0 22 6228.5 23 6965.5 24 7856.5 25 9209.0 26 11191.0 27 14023.0 28 17203.0 29 20499.0 30 24987.5 31 30763.5 32 39664.0 33 54839.5 34 75312.5 35 97608.5 36 127523.5 37 166551.0 38 195822.5 39 200217.0 40 199016.0 41 197784.5 42 189955.5 43 184452.0 44 173769.0 45 160871.0 46 156310.5 47 145361.5 48 121097.5 49 97399.0 50 78612.0 51 63301.5 52 51036.0 53 40106.0 54 31824.0 55 26471.0 56 21081.0 57 15652.5 58 11287.0 59 8021.5 60 5815.5 61 4528.0 62 3478.0 63 2619.5 64 2026.0 65 1521.0 66 1029.0 67 734.0 68 565.0 69 450.0 70 351.5 71 263.5 72 198.0 73 157.5 74 127.0 75 98.0 76 84.0 77 69.0 78 45.5 79 32.0 80 31.0 81 24.5 82 15.0 83 16.0 84 15.5 85 10.5 86 7.0 87 5.0 88 2.5 89 1.0 90 2.0 91 2.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1801617937358589 2 0.0 3 0.0 4 0.008826285793374597 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.002551119149580033 12-13 0.0 14-15 0.0 16-17 2.1992406461896836E-4 18-19 1.4661604307931225E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.002345856689268996 40-41 0.006641706751492844 42-43 0.0028003664228148637 44-45 0.0013048827834058788 46-47 0.007301478945349749 48-49 0.009148841088149083 50-51 0.005849980118864558 52-53 0.007272155736733887 54-55 0.02058489244833544 56-57 0.023267966036686853 58-59 0.03017358166572246 60-61 0.027431861660139324 62-63 0.01150935938172601 64-65 0.01712475383166367 66-67 0.02501269694933067 68-69 0.028648774817697613 70-71 0.031141247550045922 72-73 0.02407435427362307 74-75 0.03526115836057459 76-77 0.03149312605343627 78-79 0.022461577799750634 80-81 0.00982327488631392 82-83 0.0020526246031103714 84-85 0.008225160016749417 86-87 0.02995365760110349 88-89 0.027035998343825175 90-91 0.02300405715914409 92-93 0.047342320310309924 94-95 0.04870584951094753 96-97 0.03690325804306289 98-99 0.04490849399519334 100-101 0.0442780450099523 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3410268.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.041199262969045 #Duplication Level Percentage of deduplicated Percentage of total 1 80.99174577666936 34.85981868629358 2 9.194972297100321 7.915252697139502 3 3.2313105633972854 4.17238445519158 4 1.7761581693257713 3.0579191075400334 5 1.026979957735309 2.2101224499980483 6 0.6729880030490201 1.7379726444492296 7 0.42690964465385056 1.2862292157980781 8 0.3394493057212169 1.1688244165778716 9 0.2797386798201501 1.083625943372908 >10 1.7650061977895513 14.259492770465751 >50 0.15719460561905818 4.704935380893304 >100 0.12330680818671574 11.106410757955874 >500 0.01205202186319631 3.4098237432453584 >1k 0.0020605262500090612 1.237799145558566 >5k 0.0 0.0 >10k+ 1.2744281918796677E-4 7.789388585520178 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 176855 5.185956059758353 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 107155 3.1421284192327406 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT 3874 0.11359811017785112 TruSeq Adapter, Index 19 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.0526246031103714E-4 0.0 0.0 2 2.0526246031103714E-4 0.0 0.0 3 2.63908877542762E-4 0.0 0.0 4 2.932320861586245E-4 0.0 0.0 5 3.518785033903494E-4 0.0 0.0 6 4.398481292379367E-4 0.0 0.0 7 5.27817755085524E-4 0.0 0.0 8 6.451105895489738E-4 0.0 0.0 9 8.503730498600111E-4 0.0 0.0 10-11 0.0020526246031103714 0.0 0.0 12-13 0.007858619909051135 0.0 0.0 14-15 0.017887157255676092 0.0 0.0 16-17 0.036815288417215306 0.0 0.0 18-19 0.054819738507354845 0.0 0.0 20-21 0.06311820654564393 0.0 0.0 22-23 0.07084487201592368 0.0 0.0 24-25 0.07909935524128894 0.0 0.0 26-27 0.0896410487386915 0.0 0.0 28-29 0.10163424106257925 0.0 0.0 30-31 0.11510825542156805 0.0 0.0 32-33 0.12971121331226754 0.0 0.0 34-35 0.14492995858390015 0.0 0.0 36-37 0.16116035455278002 0.0 0.0 38-39 0.18020577854878267 0.0 0.0 40-41 0.19998428276018188 0.0 0.0 42-43 0.22124360900668216 0.0 0.0 44-45 0.24326533867719485 0.0 0.0 46-47 0.2679848035403669 0.0 0.0 48-49 0.29375990391371 0.0 0.0 50-51 0.3221301082495569 0.0 0.0 52-53 0.34978189397431525 0.0 0.0 54-55 0.3814949440923705 0.0 0.0 56-57 0.41455686180675533 0.0 0.0 58-59 0.4503898227353393 0.0 0.0 60-61 0.4866479701888532 0.0 0.0 62-63 0.5235365666276082 0.0 0.0 64-65 0.5664950672498467 0.0 0.0 66-67 0.6114622076622718 0.0 0.0 68-69 0.6617954952513996 0.0 0.0 70-71 0.7169817738664528 0.0 0.0 72-73 0.7782232950606814 0.0 0.0 74-75 0.8451388571220796 0.0 0.0 76-77 0.9216577700051727 0.0 0.0 78-79 1.0046277887837554 0.0 0.0 80-81 1.1016289628850284 0.0 0.0 82-83 1.2140981295311688 0.0 0.0 84-85 1.3449382863751471 0.0 0.0 86-87 1.4931964291369475 0.0 1.4661604307931225E-5 88-89 1.6616436010307694 0.0 2.932320861586245E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAG 185 0.0 64.30593 1 GCGACTA 30 1.3029345E-4 63.32013 4 CGGGTCG 90 2.2191671E-10 47.586384 1 CGCGTAG 40 5.395224E-4 47.490097 5 CGCGTTG 55 4.97149E-5 43.172813 6 GTGCGAC 45 9.542035E-4 42.299007 1 GCGACGA 45 9.637629E-4 42.213417 4 CGCTGCC 70 4.318881E-6 40.705795 2 GTCGACC 50 0.0016018988 38.06911 1 CGGTTCG 50 0.0016018988 38.06911 1 CGCGAGG 80 1.0648853E-5 35.689793 1 CGGGGTG 630 0.0 35.500954 1 GCGCAAC 95 8.720817E-7 34.992702 2 GTCCGGC 70 2.0323678E-4 33.990276 1 CGGCGTG 85 1.6129576E-5 33.59039 1 GGCGCCG 100 1.2818582E-6 33.31047 1 CCGTTGT 130 8.314601E-9 32.877758 7 CGGGTGG 380 0.0 32.559105 1 CGACGGG 105 1.8763276E-6 31.724257 1 CGCAGCC 165 7.4578566E-11 31.724257 1 >>END_MODULE