FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_50220371-2.34100000035905.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_50220371-2.34100000035905.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3410268
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1768555.185956059758353No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1071553.1421284192327406No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT38740.11359811017785112TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAG1850.064.305931
GCGACTA301.3029345E-463.320134
CGGGTCG902.2191671E-1047.5863841
CGCGTAG405.395224E-447.4900975
CGCGTTG554.97149E-543.1728136
GTGCGAC459.542035E-442.2990071
GCGACGA459.637629E-442.2134174
CGCTGCC704.318881E-640.7057952
GTCGACC500.001601898838.069111
CGGTTCG500.001601898838.069111
CGCGAGG801.0648853E-535.6897931
CGGGGTG6300.035.5009541
GCGCAAC958.720817E-734.9927022
GTCCGGC702.0323678E-433.9902761
CGGCGTG851.6129576E-533.590391
GGCGCCG1001.2818582E-633.310471
CCGTTGT1308.314601E-932.8777587
CGGGTGG3800.032.5591051
CGACGGG1051.8763276E-631.7242571
CGCAGCC1657.4578566E-1131.7242571
Produced by FastQC (version 0.11.2)