FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_50110001-2.341000000358f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_50110001-2.341000000358f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6072226
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT278970.4594196592814562TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC195970.322731729681998No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156350.25748382882982285No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79200.13042992800333847No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG78160.12871721177703202No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA64020.10543085846936527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGG3100.050.6482051
CGCGGAG4950.043.253291
CGAGCGG1650.043.2532841
CGACGGG2950.041.9336931
CGGACGG1400.040.781671
TGTACCG2000.040.366892
CCGCGCA853.597179E-739.182391
CGGGTGG6950.037.652141
AGTCGCC651.3159896E-436.5989341
GCCGGTA1203.807145E-935.617844
CGCAGCC4700.035.4308851
GCGCGGG2300.035.1016432
CGAGGGG8600.034.85411
TCAGCCG3850.034.5385132
GTTACGG2350.034.418571
AACGGCC1404.4929038E-1033.9847261
ACGTACC702.0345046E-433.9847261
CGTCCCG1553.6379788E-1133.7029044
ATTGACG1703.6379788E-1233.5849041
GTCGCTA2550.033.5204665
Produced by FastQC (version 0.11.2)