FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_44065-1.341000000357de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_44065-1.341000000357de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3948268
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT184930.46838259206315275TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC353.378762E-667.99111
CGGACGG505.48971E-966.631271
CGACGTC250.004983129457.1125221
CGCGGAC352.7617463E-454.3928761
CGAGGCG1750.051.6732331
CGGACCC851.2732926E-1050.3934021
CGGCGCC502.7954826E-547.593771
CGGTCGC502.7954826E-547.593771
CGCGGCG405.3376344E-447.5937651
CGAGGTG2450.044.6798671
CGCGTGG652.5560657E-643.9327131
CGCGAGC459.5349364E-442.305571
CACGGGT704.254818E-640.794661
CGACGGT608.1985985E-539.6614721
CGAGCGG1208.54925E-1139.6614721
AATCCGC500.001600708938.0750161
TCGCCGG756.9334674E-637.9944842
CGAGGGG4950.037.4981231
TTGTGTT258200.037.271341
GTCGACC651.3134428E-436.610591
Produced by FastQC (version 0.11.2)