##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_44065-1.341000000357de.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3948268 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66020569019124 33.0 33.0 33.0 33.0 33.0 2 32.683054189837165 33.0 33.0 33.0 33.0 33.0 3 32.732013885582234 33.0 33.0 33.0 33.0 33.0 4 36.682647935753096 37.0 37.0 37.0 37.0 37.0 5 36.638136013056865 37.0 37.0 37.0 37.0 37.0 6 36.54044431634327 37.0 37.0 37.0 37.0 37.0 7 36.51338080393732 37.0 37.0 37.0 37.0 37.0 8 36.46332847719557 37.0 37.0 37.0 37.0 37.0 9 36.460378576125024 37.0 37.0 37.0 37.0 37.0 10-11 36.430772049921636 37.0 37.0 37.0 37.0 37.0 12-13 36.42647396276038 37.0 37.0 37.0 37.0 37.0 14-15 38.33599910137812 40.0 37.0 40.0 37.0 40.0 16-17 38.32011834049766 40.0 37.0 40.0 37.0 40.0 18-19 38.30624060980663 40.0 37.0 40.0 37.0 40.0 20-21 38.23891159364055 40.0 37.0 40.0 37.0 40.0 22-23 38.1619391084901 40.0 37.0 40.0 37.0 40.0 24-25 38.12411264888807 40.0 37.0 40.0 37.0 40.0 26-27 38.05109240304863 40.0 37.0 40.0 37.0 40.0 28-29 37.94573506661655 40.0 37.0 40.0 33.0 40.0 30-31 37.90667148228033 40.0 37.0 40.0 33.0 40.0 32-33 37.800026365991364 40.0 37.0 40.0 33.0 40.0 34-35 37.72973782428143 40.0 37.0 40.0 33.0 40.0 36-37 37.66510783969072 40.0 37.0 40.0 33.0 40.0 38-39 37.57781766587273 40.0 37.0 40.0 33.0 40.0 40-41 37.487107384807715 40.0 37.0 40.0 33.0 40.0 42-43 37.37471253217866 40.0 37.0 40.0 33.0 40.0 44-45 37.25934270419333 40.0 37.0 40.0 33.0 40.0 46-47 37.19019757524059 40.0 37.0 40.0 33.0 40.0 48-49 36.9959578478462 38.5 37.0 40.0 33.0 40.0 50-51 36.852741252620135 37.0 37.0 40.0 33.0 40.0 52-53 36.72577330110317 37.0 37.0 40.0 33.0 40.0 54-55 36.4864189816902 37.0 37.0 40.0 33.0 40.0 56-57 36.35765796546739 37.0 37.0 40.0 33.0 40.0 58-59 36.14354192775161 37.0 37.0 40.0 33.0 40.0 60-61 35.87353100144165 37.0 37.0 40.0 33.0 40.0 62-63 35.56078247474589 37.0 35.0 40.0 27.0 40.0 64-65 35.32415859308436 37.0 33.0 37.0 27.0 40.0 66-67 35.03175974883165 37.0 33.0 37.0 27.0 40.0 68-69 34.7696834409417 37.0 33.0 37.0 27.0 40.0 70-71 34.3681738423025 37.0 33.0 37.0 27.0 40.0 72-73 34.138918634702605 37.0 33.0 37.0 27.0 38.5 74-75 33.784619863697195 37.0 33.0 37.0 27.0 37.0 76-77 31.134997041740835 33.0 30.0 35.0 24.5 37.0 78-79 33.44405660912582 35.0 33.0 37.0 27.0 37.0 80-81 33.824999848034636 37.0 33.0 37.0 27.0 37.0 82-83 33.818906543324815 37.0 33.0 37.0 27.0 37.0 84-85 33.68700035053345 37.0 33.0 37.0 27.0 37.0 86-87 33.55478364183992 37.0 33.0 37.0 27.0 37.0 88-89 33.3412425397668 37.0 33.0 37.0 27.0 37.0 90-91 33.10065071570623 37.0 33.0 37.0 27.0 37.0 92-93 33.10390733354474 37.0 33.0 37.0 27.0 37.0 94-95 33.046719852857 35.0 33.0 37.0 27.0 37.0 96-97 32.88329933530348 33.0 33.0 37.0 27.0 37.0 98-99 32.73067177810625 33.0 33.0 37.0 27.0 37.0 100-101 31.101051144451187 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.14359896866086785 2306 2 0.11358546667166536 2306 3 0.07869756341437295 2306 4 0.10704928124178537 2306 5 0.16547177318183515 2306 6 0.16377980286984695 2306 7 0.1825285567690429 2306 8 0.1733580373942658 2306 9 0.1712904279652534 2306 10-11 0.16037625621162022 2306 12-13 0.1691108158690966 2306 14-15 0.4106562444624302 2306 16-17 0.35484568200374866 2306 18-19 0.35882054449904643 2306 20-21 0.3581292616022651 2306 22-23 0.36475994847614857 2306 24-25 0.3376589263039804 2306 26-27 0.3472518602447181 2306 28-29 0.3808586186439342 2306 30-31 0.3812068994550373 2306 32-33 0.41017932040361416 2306 34-35 0.3997361234274308 2306 36-37 0.47537769696567267 2306 38-39 0.437744648048799 2306 40-41 0.35413047294794353 2306 42-43 0.3583455080057263 2306 44-45 0.46739588331508486 2306 46-47 0.44908032822043253 2306 48-49 0.5349169524131483 2306 50-51 0.32178428107255286 2306 52-53 0.24343844233421663 2306 54-55 0.40538565311623387 2306 56-57 0.4906251808848481 2306 58-59 0.43318605047961967 2306 60-61 0.43611523162527277 2306 62-63 0.34574134317204397 2306 64-65 0.41743731717694743 2306 66-67 0.30753962905895804 2306 68-69 0.38194907641709364 2306 70-71 0.4028686686483951 2306 72-73 0.3793590299488656 2306 74-75 0.2698206555280578 2306 76-77 -0.06775203812048503 2306 78-79 0.40186160445914965 2306 80-81 0.48279064424757223 2306 82-83 0.29052160465061405 2306 84-85 0.39344059974796153 2306 86-87 0.26890514498558815 2306 88-89 0.47531168721627637 2306 90-91 0.47357483316060467 2306 92-93 0.3783810403453387 2306 94-95 0.5000913508026343 2306 96-97 0.314292664561755 2306 98-99 0.1722151158275551 2306 100-101 0.12693056652006973 2307 1 0.15751433909303358 2307 2 0.1541061561828272 2307 3 0.10549340487824566 2307 4 0.06961933414147126 2307 5 0.12350495364240288 2307 6 0.14825434771465495 2307 7 0.10142163721931752 2307 8 0.13842907133433613 2307 9 0.197035346713605 2307 10-11 0.16013327039195246 2307 12-13 0.15438921424881613 2307 14-15 0.31090469888447103 2307 16-17 0.30442485806214137 2307 18-19 0.36716145530976263 2307 20-21 0.31959210343125477 2307 22-23 0.3673030036406004 2307 24-25 0.3306211762007081 2307 26-27 0.3705029826004065 2307 28-29 0.3903966778421264 2307 30-31 0.425542016648528 2307 32-33 0.43612266981815395 2307 34-35 0.4189742323702603 2307 36-37 0.4050577248028233 2307 38-39 0.34359438822298927 2307 40-41 0.3849725115932898 2307 42-43 0.4157111468232557 2307 44-45 0.3679399420559406 2307 46-47 0.3984781306899663 2307 48-49 0.488874525155893 2307 50-51 0.4305781451559767 2307 52-53 0.29983880563962373 2307 54-55 0.5494904067272657 2307 56-57 0.5950316028641254 2307 58-59 0.44030252157914873 2307 60-61 0.42435494538064233 2307 62-63 0.26647731160673516 2307 64-65 0.3255856277406295 2307 66-67 0.2708796797794619 2307 68-69 0.3116000038465714 2307 70-71 0.4379509933398893 2307 72-73 0.3389806895011773 2307 74-75 0.3427234310143277 2307 76-77 0.006247610813847615 2307 78-79 0.4716442269982295 2307 80-81 0.39824170987039054 2307 82-83 0.42105864207817234 2307 84-85 0.5269138838163201 2307 86-87 0.37852022980991507 2307 88-89 0.527776776030926 2307 90-91 0.5495546020196329 2307 92-93 0.4226774948302392 2307 94-95 0.3874521495452541 2307 96-97 0.3371527146537332 2307 98-99 0.30814296501074523 2307 100-101 0.19504841831246722 2201 1 0.1135675324382106 2201 2 0.14492424178170893 2201 3 0.111113203093268 2201 4 0.1491823981480067 2201 5 0.16854344634631957 2201 6 0.1471247719277784 2201 7 0.18242893493668078 2201 8 0.11972433105886893 2201 9 0.08879821388584475 2201 10-11 0.1716176114480561 2201 12-13 0.14282587212775155 2201 14-15 0.32559105936743293 2201 16-17 0.3035412289869086 2201 18-19 0.28768561463360953 2201 20-21 0.22723259790580386 2201 22-23 0.28368299693584476 2201 24-25 0.3449139257782008 2201 26-27 0.30433451790219834 2201 28-29 0.3500981047850331 2201 30-31 0.26762249406692007 2201 32-33 0.358940985448136 2201 34-35 0.31490319161225244 2201 36-37 0.3088852037869998 2201 38-39 0.20278289966982044 2201 40-41 0.3570425337520504 2201 42-43 0.3417614378529805 2201 44-45 0.2860490633467663 2201 46-47 0.3782220268359211 2201 48-49 0.4190998517721738 2201 50-51 0.3982699311801241 2201 52-53 0.2765546962686827 2201 54-55 0.4584956530134505 2201 56-57 0.4047870371084059 2201 58-59 0.45571590342672863 2201 60-61 0.4097322473505045 2201 62-63 0.329041442872942 2201 64-65 0.34202645618339744 2201 66-67 0.2695025705279619 2201 68-69 0.27620247798875397 2201 70-71 0.364829040506649 2201 72-73 0.2240707314818522 2201 74-75 0.25825701180419713 2201 76-77 0.1292615009609186 2201 78-79 0.4780152806233602 2201 80-81 0.5330294994504072 2201 82-83 0.2882266457525233 2201 84-85 0.3785955630895046 2201 86-87 0.2359502823727908 2201 88-89 0.5007180217416476 2201 90-91 0.5267256503363598 2201 92-93 0.39886884339140494 2201 94-95 0.1933374451919434 2201 96-97 0.31851226191560755 2201 98-99 0.2573951427570762 2201 100-101 0.32065184123676005 1101 1 0.12417695753314462 1101 2 0.16886029058753138 1101 3 0.11362335729129569 1101 4 0.10304532373705655 1101 5 0.19120583367401167 1101 6 0.13875620796440558 1101 7 0.15178166988067687 1101 8 0.12977369874792544 1101 9 0.17086304305660605 1101 10-11 0.17237731560639702 1101 12-13 0.17107191812066702 1101 14-15 0.2903178626792098 1101 16-17 0.25081775137684303 1101 18-19 0.16052723296133564 1101 20-21 0.23589237728577217 1101 22-23 0.26238967390631274 1101 24-25 0.29174234811416966 1101 26-27 0.3116709271006286 1101 28-29 0.2503251138782119 1101 30-31 0.361907905744296 1101 32-33 0.3105301212726985 1101 34-35 0.3118148918141017 1101 36-37 0.3565575644307728 1101 38-39 0.44225051855436703 1101 40-41 0.4454645287075607 1101 42-43 0.41396635174325525 1101 44-45 0.41356812631875783 1101 46-47 0.5158942052445212 1101 48-49 0.5507181686805822 1101 50-51 0.46793275460921535 1101 52-53 0.45115250233762083 1101 54-55 0.6001968977518573 1101 56-57 0.48634837394955355 1101 58-59 0.5091312431897776 1101 60-61 0.5691026356503599 1101 62-63 0.49487094039643864 1101 64-65 0.4307762940211646 1101 66-67 0.35367077251093093 1101 68-69 0.44232846853164887 1101 70-71 0.5114307875465514 1101 72-73 0.5152020672891311 1101 74-75 0.5984752862391858 1101 76-77 0.11626014899244552 1101 78-79 0.45199272929122714 1101 80-81 0.5885355818650879 1101 82-83 0.6101092650096547 1101 84-85 0.5445615565625985 1101 86-87 0.5178492414490918 1101 88-89 0.6217063895441939 1101 90-91 0.7263158377120149 1101 92-93 0.5288171824260459 1101 94-95 0.47809208357330846 1101 96-97 0.5045083819302789 1101 98-99 0.4909370962712245 1101 100-101 0.5840345338390414 2304 1 0.18603273677491217 2304 2 0.14062451837209977 2304 3 0.12488947548304452 2304 4 0.12952229372403679 2304 5 0.18522022014862216 2304 6 0.13393126218795004 2304 7 0.1590178506135871 2304 8 0.13336600598380244 2304 9 0.10331994915777187 2304 10-11 0.11563837791405973 2304 12-13 0.1527247021651803 2304 14-15 0.26397574188012385 2304 16-17 0.26056987901870343 2304 18-19 0.23651195432210415 2304 20-21 0.19275937688492917 2304 22-23 0.20299477495356655 2304 24-25 0.19969879169841676 2304 26-27 0.1768766350759421 2304 28-29 0.22806479561021575 2304 30-31 0.17498985640033737 2304 32-33 0.24612612352147067 2304 34-35 0.3431989806344262 2304 36-37 0.24712123745115377 2304 38-39 0.2688532453338581 2304 40-41 0.2687769182274877 2304 42-43 0.31869794038962596 2304 44-45 0.27343092079177467 2304 46-47 0.3010178084626318 2304 48-49 0.3039147432806999 2304 50-51 0.26672235615312445 2304 52-53 0.2909403549827587 2304 54-55 0.4192604920141676 2304 56-57 0.40638741575621395 2304 58-59 0.3730619668206572 2304 60-61 0.3295764988554808 2304 62-63 0.3211504816103883 2304 64-65 0.2847419230591086 2304 66-67 0.36833112885599917 2304 68-69 0.39938504244023676 2304 70-71 0.41813333431368704 2304 72-73 0.2573133471099496 2304 74-75 0.2976141979776301 2304 76-77 0.2572570340999967 2304 78-79 0.4021511205263337 2304 80-81 0.49860286985966695 2304 82-83 0.4082875843849578 2304 84-85 0.44967929454537625 2304 86-87 0.3253673718135488 2304 88-89 0.45015226866143365 2304 90-91 0.5872427412562118 2304 92-93 0.333427112260928 2304 94-95 0.3875497542040591 2304 96-97 0.4423234409006582 2304 98-99 0.4021436229890014 2304 100-101 0.4083650155182923 2202 1 0.11806892795859625 2202 2 0.14452923586038224 2202 3 0.08389516333586045 2202 4 0.04741064820238705 2202 5 0.14139855583360372 2202 6 0.1362508650073906 2202 7 0.09033395227263696 2202 8 0.09832520921764143 2202 9 0.08412753011345586 2202 10-11 0.10393106875221747 2202 12-13 0.1595964859611101 2202 14-15 0.24803994392923556 2202 16-17 0.1957365055353577 2202 18-19 0.1936737873621226 2202 20-21 0.13548120769395666 2202 22-23 0.1374604573713043 2202 24-25 0.1635165478149645 2202 26-27 0.15501952086912496 2202 28-29 0.16075153570196932 2202 30-31 0.16009511075166927 2202 32-33 0.17206113429472225 2202 34-35 0.29459717028410637 2202 36-37 0.35689426526463564 2202 38-39 0.2897228669624994 2202 40-41 0.30247516179289846 2202 42-43 0.34943516981709877 2202 44-45 0.2829336579151942 2202 46-47 0.3951831213024448 2202 48-49 0.3707234974870488 2202 50-51 0.2736927547142187 2202 52-53 0.391102735091458 2202 54-55 0.4337773528447215 2202 56-57 0.47530750694224366 2202 58-59 0.4004624333519615 2202 60-61 0.48848610356969147 2202 62-63 0.3719629952821606 2202 64-65 0.3744084564198218 2202 66-67 0.42312810625212904 2202 68-69 0.4479827784540831 2202 70-71 0.48423406377813905 2202 72-73 0.2404339249220655 2202 74-75 0.2612058877552812 2202 76-77 0.08925952300534234 2202 78-79 0.5208053315904166 2202 80-81 0.44777781672517847 2202 82-83 0.37980314657020386 2202 84-85 0.32304460548647285 2202 86-87 0.40542713402261654 2202 88-89 0.5009223380319554 2202 90-91 0.6186952342952239 2202 92-93 0.4376382356741644 2202 94-95 0.36669271159480843 2202 96-97 0.28186578611245494 2202 98-99 0.26157434720428796 2202 100-101 0.3226361503475772 1102 1 0.09821788619344574 1102 2 0.11311739054332293 1102 3 0.08115617996222824 1102 4 0.025561289443253088 1102 5 0.1561518455818529 1102 6 0.046754575787872454 1102 7 0.13028846424767693 1102 8 0.07422023910967823 1102 9 0.06270517368194817 1102 10-11 0.060414036831630824 1102 12-13 0.10656083553377727 1102 14-15 0.1805122656967555 1102 16-17 0.13371595208075604 1102 18-19 0.17912789767500925 1102 20-21 0.17550253235788915 1102 22-23 0.12568446830208302 1102 24-25 0.2517974449779956 1102 26-27 0.23931119519960475 1102 28-29 0.20604174269853814 1102 30-31 0.3086557633106395 1102 32-33 0.22253376014419501 1102 34-35 0.24861098842079343 1102 36-37 0.2566474213875125 1102 38-39 0.2674429500183422 1102 40-41 0.2923382732609525 1102 42-43 0.2033006226113656 1102 44-45 0.26708916910862257 1102 46-47 0.34584777093707686 1102 48-49 0.3232029070140996 1102 50-51 0.12133751572728357 1102 52-53 0.22845916547775857 1102 54-55 0.316919751608161 1102 56-57 0.28907583916502233 1102 58-59 0.23548760620218445 1102 60-61 0.21527763997032423 1102 62-63 0.20477414160000507 1102 64-65 0.16987969398770275 1102 66-67 0.22788230255687125 1102 68-69 0.16386108907896357 1102 70-71 0.2018751064872859 1102 72-73 0.27780359058387916 1102 74-75 0.2714271062525384 1102 76-77 -0.032559023313741164 1102 78-79 0.4712907285824244 1102 80-81 0.5699129840724382 1102 82-83 0.43356744832220784 1102 84-85 0.3702733536427587 1102 86-87 0.4953080547216828 1102 88-89 0.5586510090448584 1102 90-91 0.5690596198456817 1102 92-93 0.45573975817251977 1102 94-95 0.30427715852910353 1102 96-97 0.18743894503360536 1102 98-99 0.2295916557705837 1102 100-101 0.26577832752494857 2305 1 0.08509745306690064 2305 2 0.12571398849114956 2305 3 0.05979685229850418 2305 4 0.056590832919361844 2305 5 0.08960878945647721 2305 6 0.048435288003858545 2305 7 0.08766752391677812 2305 8 0.10684155916575833 2305 9 0.05236565653821401 2305 10-11 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-1.4241977702525048 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 12.0 8 47.0 9 231.0 10 311.0 11 205.0 12 126.0 13 208.0 14 431.0 15 845.0 16 1739.0 17 2622.0 18 3860.0 19 4864.0 20 6336.0 21 7917.0 22 10213.0 23 12795.0 24 16281.0 25 20453.0 26 26356.0 27 33761.0 28 43964.0 29 57417.0 30 68137.0 31 84799.0 32 114046.0 33 162140.0 34 248362.0 35 432300.0 36 862283.0 37 1374468.0 38 349967.0 39 772.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.92739276690948 32.27773053899661 15.808692832299451 18.986183861794466 2 30.813283191515872 24.615806221867413 29.881887450395972 14.689023136220744 3 32.67366855542734 22.966272806202618 24.22198797042146 20.13807066794858 4 28.436893265364276 22.810499319254028 28.663065573251433 20.08954184213026 5 31.448295809706938 21.167939967600983 27.81845102713392 19.565313195558154 6 28.434569284557178 21.50517644698891 30.193112524276465 19.867141744177445 7 31.20084553530814 22.29998064974313 24.618490943370613 21.88068287157812 8 27.65288475858275 25.228277310456125 25.317632946902286 21.801204984058835 9 26.738762414304198 22.648943790036544 28.350608418678775 22.261685376980488 10-11 21.982415708694354 29.263423199988452 31.058755381906057 17.695405709411137 12-13 22.070386306096747 28.61158361083898 30.138860887862727 19.179169195201542 14-15 22.073894173343856 28.445827385577676 30.424619098804843 19.055659342273625 16-17 22.373730690057574 27.821336374337285 30.651622899468943 19.153310036136194 18-19 21.568862600005875 27.855809180126577 31.031024236450005 19.54430398341754 20-21 21.97134287743385 28.098890450192336 30.55658835722398 19.373178315149833 22-23 21.56251804588746 28.904192927126527 30.00147153131449 19.53181749567152 24-25 21.334797941781055 28.1936661847676 30.497486492811532 19.974049380639812 26-27 21.310445491542115 28.322254213746383 30.48439214359309 19.882908151118414 28-29 21.327389630085904 29.141461521862244 30.09171363240793 19.439435215643922 30-31 20.685424596304 29.45725822056659 30.297474740823066 19.559842442306348 32-33 20.55324005361338 29.811780760576532 29.997596414427797 19.637382771382285 34-35 21.020888146397358 29.431310133962533 30.30675729205819 19.241044427581915 36-37 20.59551175350812 28.978921896892512 31.15162141982257 19.273944929776803 38-39 20.96137701911045 29.055637147288476 30.436222457407915 19.54676337619316 40-41 20.821087220522987 29.115342963557822 30.332990841108643 19.73057897481055 42-43 21.068964311965225 29.115450800094628 30.029218688816407 19.78636619912374 44-45 20.64832833972715 28.854098769431406 30.38083914112591 20.116733749715536 46-47 21.03965095136422 29.009861604206797 30.03143246237926 19.91905498204972 48-49 20.69584697020241 29.750411417406102 29.92961893768821 19.62412267470327 50-51 21.19694340089077 29.369247790300424 29.345362569046777 20.08844623976203 52-53 20.7285124331181 29.122842833999158 29.299501722895926 20.84914300998682 54-55 21.26018260997548 29.180611008529617 29.560176606234922 19.999029775259977 56-57 20.94484498735883 28.883701252970294 29.68723875341362 20.48421500625725 58-59 20.96989290407475 29.326544401373223 29.660831235437495 20.04273145911453 60-61 21.032645229985718 29.50247377985886 29.33404217237633 20.130838817779093 62-63 20.596104401226434 29.459577586926404 29.256265189378915 20.688052822468244 64-65 20.89051827101981 29.24258640277938 29.29993705107729 20.566958275123522 66-67 21.02121338059381 29.459381986898276 29.081076931865695 20.438327700642215 68-69 21.062775888416436 29.650974552397013 29.146901841485512 20.139347717701042 70-71 21.003540506818165 30.13486860856182 28.958064945182315 19.9035259394377 72-73 20.672908177140407 30.13185806261532 28.94080478777298 20.254428972471295 74-75 20.864144060239944 29.521242062515334 28.939815412429976 20.674798464814746 76-77 20.90189668776659 29.213242751991586 29.00186348676856 20.882997073473263 78-79 20.623058878367356 28.81612479923799 29.688685889154037 20.872130433240617 80-81 20.560642792859085 29.24621640331549 29.38839336487249 20.80474743895294 82-83 20.121014547630907 29.530168534900564 29.26650428055498 21.082312636913553 84-85 20.153524586198415 29.521346049751685 29.3477377090538 20.977391654996104 86-87 20.31372396020701 29.305882507927084 29.84279094088307 20.537602590982836 88-89 20.49298763870154 29.80334937011681 29.481152778768262 20.222510212413386 90-91 20.713637554282045 29.488987540779615 28.93523596824067 20.862138936697676 92-93 20.369780553492603 29.69995015976999 29.052014500542363 20.87825478619504 94-95 19.984687868677 29.83325410789795 29.000221459585905 21.181836563839145 96-97 20.047829233573143 30.233406305133432 28.864212785617077 20.854551675676348 98-99 20.35301218159719 29.962644655991568 28.77895685271535 20.905386309695896 100-101 20.43525432867527 30.00764688712404 28.333173706464937 21.22392507773575 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 162.0 1 110.0 2 78.5 3 136.0 4 138.5 5 86.5 6 67.5 7 73.5 8 77.5 9 75.0 10 83.0 11 90.5 12 83.0 13 85.0 14 97.0 15 118.5 16 158.5 17 194.5 18 234.0 19 300.0 20 485.0 21 779.0 22 1227.5 23 1881.5 24 2839.5 25 4510.0 26 7395.5 27 11864.5 28 17116.5 29 22596.0 30 28395.0 31 32756.0 32 39923.5 33 55796.5 34 78783.5 35 111636.0 36 155705.0 37 208469.5 38 261859.5 39 281464.5 40 286483.5 41 275456.0 42 242113.5 43 227520.0 44 214776.5 45 204632.0 46 208603.5 47 195044.0 48 160443.5 49 131071.5 50 105474.0 51 85126.0 52 70237.0 53 56188.5 54 45656.5 55 35324.5 56 24275.5 57 15426.0 58 10146.5 59 7354.5 60 5374.5 61 4003.0 62 2882.0 63 1911.5 64 1346.0 65 964.0 66 708.5 67 532.0 68 394.0 69 276.0 70 184.5 71 133.5 72 97.0 73 62.0 74 39.5 75 37.5 76 34.0 77 22.5 78 15.5 79 11.5 80 6.5 81 4.5 82 6.5 83 4.5 84 1.0 85 0.5 86 1.5 87 1.5 88 1.5 89 2.0 90 2.5 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18481521517789573 2 0.0 3 0.0 4 0.00995373161092408 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0021528427148309083 12-13 0.0 14-15 0.0 16-17 2.659393941849945E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.002216161618208288 40-41 0.006243243873009633 42-43 0.0027860317486047047 44-45 0.0013296969709249726 46-47 0.007079053397591045 48-49 0.008383422807165065 50-51 0.005572063497209409 52-53 0.00668647619665129 54-55 0.018463792224843904 56-57 0.02147777202560718 58-59 0.02682188747065802 60-61 0.024529743168396873 62-63 0.010194343443758124 64-65 0.01577907072164301 66-67 0.023415330468954994 68-69 0.025834112577970896 70-71 0.027683024556590382 72-73 0.02227559020816216 74-75 0.031722770592067205 76-77 0.02779699858266967 78-79 0.020464669571569102 80-81 0.00939652526120314 82-83 0.001646291487811871 84-85 0.00762359596663651 86-87 0.026340663804989936 88-89 0.025036294395415916 90-91 0.021021875921290043 92-93 0.04196776915852723 94-95 0.043664715769041004 96-97 0.03316644158907146 98-99 0.03994156425045108 100-101 0.03961230595288871 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3948268.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.7753006016471 #Duplication Level Percentage of deduplicated Percentage of total 1 87.1648201452365 36.41336563455761 2 5.750843508218323 4.804864325377025 3 1.9670599523821348 2.4652356243667595 4 1.065283704106853 1.7801018786039946 5 0.5967323437211097 1.2464336518837378 6 0.4498187636718794 1.127478844119241 7 0.31105580457484394 0.9096114818000911 8 0.28890656412599025 0.9655326849721823 9 0.17822077109311438 0.6700703657284972 >10 1.802296273776659 15.17555873511624 >50 0.22605183722715266 6.619608454004173 >100 0.16236733993275226 13.554336232070026 >500 0.02618280094950674 7.650461417542402 >1k 0.01029931834441538 6.147068713484936 >5k 0.0 0.0 >10k+ 6.087263871912616E-5 0.47027195637312375 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 18493 0.46838259206315275 TruSeq Adapter, Index 2 (97% over 37bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.358095245814114E-4 0.0 0.0 2 9.624473313361707E-4 0.0 0.0 3 0.001418343435653304 0.0 0.0 4 0.001646291487811871 0.0 0.0 5 0.0020262049080761488 0.0 0.0 6 0.002887341994008512 0.0 0.0 7 0.0038497893253446828 0.0 0.0 8 0.005090839831541324 0.0 0.0 9 0.005572063497209409 0.0 2.532756135095186E-5 10-11 0.007547613282583654 0.0 2.532756135095186E-5 12-13 0.010878187600233825 0.0 2.532756135095186E-5 14-15 0.015323174617325876 0.0 2.532756135095186E-5 16-17 0.022313581550188588 0.0 2.532756135095186E-5 18-19 0.03117822802302174 0.0 2.532756135095186E-5 20-21 0.04053676194219845 0.0 2.532756135095186E-5 22-23 0.0520861299182325 0.0 2.532756135095186E-5 24-25 0.06553506499558794 0.0 2.532756135095186E-5 26-27 0.08047832619264954 0.0 2.532756135095186E-5 28-29 0.0962320693529416 0.0 2.532756135095186E-5 30-31 0.11563298134777072 0.0 2.532756135095186E-5 32-33 0.1344640232122034 0.0 2.532756135095186E-5 34-35 0.15501733924850086 0.0 2.532756135095186E-5 36-37 0.1787366004536673 0.0 2.532756135095186E-5 38-39 0.204912635109876 0.0 2.532756135095186E-5 40-41 0.23448256298711234 0.0 2.532756135095186E-5 42-43 0.2676996596988857 0.0 2.532756135095186E-5 44-45 0.3047665457360037 0.0 2.532756135095186E-5 46-47 0.34397361070727717 0.0 2.532756135095186E-5 48-49 0.3857007680329704 0.0 2.532756135095186E-5 50-51 0.43253142897088037 0.0 2.532756135095186E-5 52-53 0.4838324044872334 0.0 2.532756135095186E-5 54-55 0.5377547826034099 0.0 2.532756135095186E-5 56-57 0.5942225806353572 0.0 2.532756135095186E-5 58-59 0.6560218303316796 0.0 2.532756135095186E-5 60-61 0.7201638794529652 0.0 2.532756135095186E-5 62-63 0.7904605259825321 0.0 2.532756135095186E-5 64-65 0.8635811956027302 0.0 2.532756135095186E-5 66-67 0.9389180268411363 0.0 2.532756135095186E-5 68-69 1.0221823847823908 0.0 3.799134202642779E-5 70-71 1.1100563588895183 0.0 7.598268405285558E-5 72-73 1.2059338423835464 0.0 7.598268405285558E-5 74-75 1.3061423388685873 0.0 7.598268405285558E-5 76-77 1.413784474610133 0.0 7.598268405285558E-5 78-79 1.5300253174303264 0.0 7.598268405285558E-5 80-81 1.6545356090316057 0.0 7.598268405285558E-5 82-83 1.7860616351270988 0.0 7.598268405285558E-5 84-85 1.9317584317984493 0.0 1.0131024540380744E-4 86-87 2.0885233727801658 0.0 1.0131024540380744E-4 88-89 2.2571289486934525 0.0 1.0131024540380744E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGCGC 35 3.378762E-6 67.9911 1 CGGACGG 50 5.48971E-9 66.63127 1 CGACGTC 25 0.0049831294 57.112522 1 CGCGGAC 35 2.7617463E-4 54.392876 1 CGAGGCG 175 0.0 51.673233 1 CGGACCC 85 1.2732926E-10 50.393402 1 CGGCGCC 50 2.7954826E-5 47.59377 1 CGGTCGC 50 2.7954826E-5 47.59377 1 CGCGGCG 40 5.3376344E-4 47.593765 1 CGAGGTG 245 0.0 44.679867 1 CGCGTGG 65 2.5560657E-6 43.932713 1 CGCGAGC 45 9.5349364E-4 42.30557 1 CACGGGT 70 4.254818E-6 40.79466 1 CGACGGT 60 8.1985985E-5 39.661472 1 CGAGCGG 120 8.54925E-11 39.661472 1 AATCCGC 50 0.0016007089 38.075016 1 TCGCCGG 75 6.9334674E-6 37.994484 2 CGAGGGG 495 0.0 37.498123 1 TTGTGTT 25820 0.0 37.27134 1 GTCGACC 65 1.3134428E-4 36.61059 1 >>END_MODULE