##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_41442-1.34100000035721.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3136301 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66201777189116 33.0 33.0 33.0 33.0 33.0 2 32.67127740609081 33.0 33.0 33.0 33.0 33.0 3 32.73450921961891 33.0 33.0 33.0 33.0 33.0 4 36.672394326947575 37.0 37.0 37.0 37.0 37.0 5 36.63507743676388 37.0 37.0 37.0 37.0 37.0 6 36.54722968235511 37.0 37.0 37.0 37.0 37.0 7 36.440952574386195 37.0 37.0 37.0 37.0 37.0 8 36.402117972732846 37.0 37.0 37.0 37.0 37.0 9 36.43567788933524 37.0 37.0 37.0 37.0 37.0 10-11 36.38013985264807 37.0 37.0 37.0 37.0 37.0 12-13 36.37134525672121 37.0 37.0 37.0 37.0 37.0 14-15 38.22123482408098 40.0 37.0 40.0 37.0 40.0 16-17 38.26001490290632 40.0 37.0 40.0 37.0 40.0 18-19 38.22015217927106 40.0 37.0 40.0 37.0 40.0 20-21 38.20886706983801 40.0 37.0 40.0 37.0 40.0 22-23 37.98752064932543 40.0 37.0 40.0 33.0 40.0 24-25 38.056739292561524 40.0 37.0 40.0 37.0 40.0 26-27 37.99654162658494 40.0 37.0 40.0 37.0 40.0 28-29 37.78457632095899 40.0 37.0 40.0 33.0 40.0 30-31 37.75648223815253 40.0 37.0 40.0 33.0 40.0 32-33 37.622190121420104 40.0 37.0 40.0 33.0 40.0 34-35 37.62869842531058 40.0 37.0 40.0 33.0 40.0 36-37 37.55898477218864 40.0 37.0 40.0 33.0 40.0 38-39 37.26923388412018 40.0 37.0 40.0 33.0 40.0 40-41 37.11944437093251 40.0 37.0 40.0 33.0 40.0 42-43 36.900472722484224 37.0 37.0 40.0 33.0 40.0 44-45 36.722715389881266 37.0 37.0 40.0 33.0 40.0 46-47 36.860677594401814 37.0 37.0 40.0 33.0 40.0 48-49 36.44513441152492 37.0 37.0 40.0 33.0 40.0 50-51 36.38897876830062 37.0 37.0 40.0 33.0 40.0 52-53 36.39360778828308 37.0 37.0 40.0 33.0 40.0 54-55 35.90084306321364 37.0 37.0 40.0 30.0 40.0 56-57 35.80229655890809 37.0 37.0 40.0 27.0 40.0 58-59 35.66516016798133 37.0 37.0 40.0 27.0 40.0 60-61 35.27678178848268 37.0 33.0 40.0 27.0 40.0 62-63 35.00793992668433 37.0 33.0 38.5 27.0 40.0 64-65 34.77476667577506 37.0 33.0 37.0 27.0 40.0 66-67 34.052283884741925 37.0 33.0 37.0 27.0 40.0 68-69 33.99630169425703 37.0 33.0 37.0 27.0 40.0 70-71 33.58144562655179 37.0 33.0 37.0 27.0 38.5 72-73 32.84959224258131 37.0 33.0 37.0 22.0 37.0 74-75 31.9156474458287 37.0 33.0 37.0 18.5 37.0 76-77 29.448620684047864 33.0 27.0 35.0 15.0 37.0 78-79 31.378711737170633 33.0 33.0 37.0 15.0 37.0 80-81 31.647420480368435 37.0 33.0 37.0 15.0 37.0 82-83 31.598336224743733 37.0 33.0 37.0 15.0 37.0 84-85 31.450233730754796 37.0 33.0 37.0 15.0 37.0 86-87 31.32185096392215 33.0 33.0 37.0 15.0 37.0 88-89 31.125471853626294 33.0 33.0 37.0 15.0 37.0 90-91 30.87640216930709 33.0 33.0 37.0 15.0 37.0 92-93 30.857251265104974 33.0 33.0 37.0 15.0 37.0 94-95 30.794562766775258 33.0 33.0 37.0 15.0 37.0 96-97 30.63026555805709 33.0 33.0 37.0 15.0 37.0 98-99 30.481348410117526 33.0 33.0 37.0 15.0 37.0 100-101 29.010465035084323 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.15701614314084367 2306 2 0.17826687251253048 2306 3 0.05622465005055943 2306 4 0.11537608761231866 2306 5 0.15673795723904504 2306 6 0.16424480506305628 2306 7 0.10792870437221325 2306 8 0.1626309872723226 2306 9 0.23591955866840664 2306 10-11 0.18783310476139548 2306 12-13 0.22909608632769363 2306 14-15 0.3855277791370568 2306 16-17 0.46929501919725425 2306 18-19 0.44281691799043443 2306 20-21 0.3673927082481967 2306 22-23 0.4208025386249048 2306 24-25 0.4068152354325534 2306 26-27 0.4398346758395846 2306 28-29 0.46173462236390606 2306 30-31 0.49120870095943303 2306 32-33 0.3616868835989635 2306 34-35 0.3454098265278134 2306 36-37 0.49700204471531606 2306 38-39 0.5573697480367272 2306 40-41 0.5341714511521545 2306 42-43 0.5088105803058767 2306 44-45 0.4618652372279257 2306 46-47 0.2479147170714313 2306 48-49 0.019529908450387268 2306 50-51 -0.03594320000644302 2306 52-53 0.05210943279001157 2306 54-55 0.030896080814073912 2306 56-57 -0.0023862256971014517 2306 58-59 0.07084801803823382 2306 60-61 -0.08505030539878078 2306 62-63 -0.15286138014367623 2306 64-65 -0.0639350306600619 2306 66-67 -0.10267281784182813 2306 68-69 0.29147121606039406 2306 70-71 0.5288371370888072 2306 72-73 0.42216034580369666 2306 74-75 0.27148003817520916 2306 76-77 -0.07486327282866867 2306 78-79 0.3822800382057423 2306 80-81 0.4815550540027118 2306 82-83 0.49129030228852244 2306 84-85 0.5482836476653503 2306 86-87 0.5937054010997826 2306 88-89 0.7188834266524431 2306 90-91 0.657547187920958 2306 92-93 0.49746896785269357 2306 94-95 0.5770819675122176 2306 96-97 0.5328415650971614 2306 98-99 0.5045194741315768 2306 100-101 0.4399800878515201 2307 1 0.1595199656621702 2307 2 0.14662023263667123 2307 3 0.12908062767814954 2307 4 0.10611961774628753 2307 5 0.2094596208457986 2307 6 0.1340610966773852 2307 7 0.10941863508368499 2307 8 0.07696032661559116 2307 9 0.131652829098293 2307 10-11 0.1743782559854523 2307 12-13 0.12752856838032045 2307 14-15 0.3317918684675334 2307 16-17 0.25952634568320576 2307 18-19 0.3358580818558181 2307 20-21 0.31533323606876706 2307 22-23 0.3508969364285832 2307 24-25 0.36126754912371695 2307 26-27 0.3122214354214634 2307 28-29 0.35762207255744727 2307 30-31 0.31082796930895995 2307 32-33 0.2719908522002896 2307 34-35 0.353977105745237 2307 36-37 0.2856194572236319 2307 38-39 0.3193698361394439 2307 40-41 0.4171596681235812 2307 42-43 0.30961707546591555 2307 44-45 0.35206047001804563 2307 46-47 0.2583609289385791 2307 48-49 0.17845549575449837 2307 50-51 0.12656404715294656 2307 52-53 -0.018553923866861055 2307 54-55 0.2526996572161906 2307 56-57 0.3740568116300338 2307 58-59 0.2934690313900603 2307 60-61 0.17423280843561884 2307 62-63 0.026685572923149437 2307 64-65 -0.02357723413755508 2307 66-67 0.008148327948283907 2307 68-69 0.23770688515794092 2307 70-71 0.3328082251858433 2307 72-73 0.16906325091990482 2307 74-75 0.14031320288183124 2307 76-77 0.1533920517019709 2307 78-79 0.4921444879047705 2307 80-81 0.5979149479384063 2307 82-83 0.6389000185977558 2307 84-85 0.5057737290040549 2307 86-87 0.41146492045680816 2307 88-89 0.5717568543030573 2307 90-91 0.6512734215011697 2307 92-93 0.582781888827391 2307 94-95 0.42599823297992856 2307 96-97 0.395597062991218 2307 98-99 0.4050674872730937 2307 100-101 0.3481475654421651 2201 1 0.18455601041858927 2201 2 0.17936240940151293 2201 3 0.09829637500610033 2201 4 0.1403323528833269 2201 5 0.21880621233179198 2201 6 0.15913470920809658 2201 7 0.1281395708258657 2201 8 0.07828332147794725 2201 9 0.1698873467754538 2201 10-11 0.20035569501396822 2201 12-13 0.19003085290713528 2201 14-15 0.27424548411197947 2201 16-17 0.40658032143675626 2201 18-19 0.35847286663646827 2201 20-21 0.4102614173146577 2201 22-23 0.36883537943048594 2201 24-25 0.39518938784627977 2201 26-27 0.459005667786613 2201 28-29 0.41125751622947604 2201 30-31 0.4899546708583742 2201 32-33 0.5131270573812543 2201 34-35 0.4405523800576745 2201 36-37 0.49516048732523643 2201 38-39 0.41409748669822477 2201 40-41 0.4392728723501236 2201 42-43 0.6137574841762188 2201 44-45 0.528796541173449 2201 46-47 0.5771784917038403 2201 48-49 0.5590930310377544 2201 50-51 0.48464230319594037 2201 52-53 0.42003850301136225 2201 54-55 0.43442919631713295 2201 56-57 0.5414891050242048 2201 58-59 0.5522710593729769 2201 60-61 0.4194419018676925 2201 62-63 0.3754299292957981 2201 64-65 0.4039262688035592 2201 66-67 0.33496579594798703 2201 68-69 0.47170944382796876 2201 70-71 0.4667705084942426 2201 72-73 0.3313562051007608 2201 74-75 0.18483304611251938 2201 76-77 0.19135056469500356 2201 78-79 0.3809772131591167 2201 80-81 0.41460603929916573 2201 82-83 0.459752502470689 2201 84-85 0.3483243101199882 2201 86-87 0.4007239214924567 2201 88-89 0.440734164721178 2201 90-91 0.4712835072639976 2201 92-93 0.5150967744656718 2201 94-95 0.36357751452163 2201 96-97 0.3584807788840827 2201 98-99 0.27582063653561306 2201 100-101 0.4272639443148627 1101 1 0.17575718116491146 1101 2 0.18207635303573966 1101 3 0.13770618342498864 1101 4 0.057763482819567 1101 5 0.14431780340403577 1101 6 0.1654603531429899 1101 7 0.09345165318181614 1101 8 0.09346281583664506 1101 9 0.13593944759116283 1101 10-11 0.11206861147783798 1101 12-13 0.09335401763367912 1101 14-15 0.24681100081448193 1101 16-17 0.20877994735791816 1101 18-19 0.207356164781217 1101 20-21 0.22579895624051005 1101 22-23 0.22739874086585843 1101 24-25 0.24046472973448374 1101 26-27 0.31305509568039014 1101 28-29 0.2406214031332965 1101 30-31 0.3042946040190486 1101 32-33 0.23693592230102922 1101 34-35 0.18033569757378132 1101 36-37 0.281281559945036 1101 38-39 0.260327234268253 1101 40-41 0.28584389768102625 1101 42-43 0.2879147893760461 1101 44-45 0.443991093100891 1101 46-47 0.4987354768692853 1101 48-49 0.4457766055906234 1101 50-51 0.28936875193753764 1101 52-53 0.2789179919981777 1101 54-55 0.4652347843843785 1101 56-57 0.36086632398077967 1101 58-59 0.48669933569644996 1101 60-61 0.35173444519686825 1101 62-63 0.4471728957833463 1101 64-65 0.40044064511905475 1101 66-67 0.25712907208426117 1101 68-69 0.34459027415928034 1101 70-71 0.4059754525858992 1101 72-73 0.10875739896248149 1101 74-75 0.1847526305561793 1101 76-77 0.07449032009497358 1101 78-79 0.28138091878608407 1101 80-81 0.49047901202136046 1101 82-83 0.42125925233110095 1101 84-85 0.28226358172434374 1101 86-87 0.430846221507597 1101 88-89 0.4678288353185458 1101 90-91 0.5294351866305682 1101 92-93 0.3486961519776379 1101 94-95 0.22049365932823406 1101 96-97 0.23619443764539128 1101 98-99 0.3225938402490556 1101 100-101 0.2757192005458471 2304 1 0.12390307858349559 2304 2 0.13209235030979727 2304 3 0.06056898037527958 2304 4 0.0786901395203401 2304 5 0.11416415264945812 2304 6 0.09825969376469601 2304 7 0.15683476182680778 2304 8 0.10733258064680484 2304 9 0.0975911469062325 2304 10-11 0.09361348376032197 2304 12-13 0.10442440193578051 2304 14-15 0.2302634376280963 2304 16-17 0.18365553756900255 2304 18-19 0.18826762763291782 2304 20-21 0.27878355184850534 2304 22-23 0.2089565762110297 2304 24-25 0.2569777484955935 2304 26-27 0.23878280070832147 2304 28-29 0.20493551609086325 2304 30-31 0.12457078563070212 2304 32-33 0.15481278346207006 2304 34-35 0.2740827862266002 2304 36-37 0.20922708830095615 2304 38-39 0.14996534544460616 2304 40-41 0.2010892571182623 2304 42-43 0.2990050456258828 2304 44-45 0.4148950715645441 2304 46-47 0.3946925112966184 2304 48-49 0.37077652724244103 2304 50-51 0.3825768051049039 2304 52-53 0.39753920361432904 2304 54-55 0.5923148217521899 2304 56-57 0.5332834622322764 2304 58-59 0.42352840817080306 2304 60-61 0.3498813682643629 2304 62-63 0.33366660955265814 2304 64-65 0.3918133707782019 2304 66-67 0.4450082238612154 2304 68-69 0.22783602847135143 2304 70-71 0.2851249503170763 2304 72-73 0.028548519862845012 2304 74-75 0.23633429514448423 2304 76-77 0.28022950954173 2304 78-79 0.45171641709334054 2304 80-81 0.6021675492266354 2304 82-83 0.39347470811950913 2304 84-85 0.5325645099650984 2304 86-87 0.5420067631983727 2304 88-89 0.5495807462306814 2304 90-91 0.7238713689031968 2304 92-93 0.40979177863915695 2304 94-95 0.5029602908379367 2304 96-97 0.5475922033831857 2304 98-99 0.4473993586960603 2304 100-101 0.4181368202825233 2202 1 0.09188598324633546 2202 2 0.1231956142274484 2202 3 0.11624076175839804 2202 4 0.08656931458217798 2202 5 0.0936588745148157 2202 6 0.10974225950054262 2202 7 0.10375778054697093 2202 8 0.10093821003305692 2202 9 0.13517772149707952 2202 10-11 0.13704411137199202 2202 12-13 0.11278505613898204 2202 14-15 0.21182097292291502 2202 16-17 0.18949218021827363 2202 18-19 0.14677520220271134 2202 20-21 0.12300779125187944 2202 22-23 0.08061245103659331 2202 24-25 0.17652587963888777 2202 26-27 0.1259074361375383 2202 28-29 0.17032011047442808 2202 30-31 0.1850704240825678 2202 32-33 0.2292253945103866 2202 34-35 0.2752136479280054 2202 36-37 0.2419054617946017 2202 38-39 0.3668897370336097 2202 40-41 0.3314193618697132 2202 42-43 0.3114743608373445 2202 44-45 0.22984190799893156 2202 46-47 0.3406034292700397 2202 48-49 0.4286794154932565 2202 50-51 0.44569698495071464 2202 52-53 0.4417878972438274 2202 54-55 0.448677249764323 2202 56-57 0.593431498054791 2202 58-59 0.4712681668423002 2202 60-61 0.421212629813823 2202 62-63 0.36583191232093526 2202 64-65 0.38338699827988876 2202 66-67 0.32638982133450867 2202 68-69 0.2926048209786103 2202 70-71 0.2595123661021077 2202 72-73 -0.06397659934663835 2202 74-75 0.08232083790973732 2202 76-77 -0.03553422285181185 2202 78-79 0.2638857292626504 2202 80-81 0.3913278527242525 2202 82-83 0.30264032368347316 2202 84-85 0.3899058870617509 2202 86-87 0.281642578538154 2202 88-89 0.4339485284312836 2202 90-91 0.5243789013508753 2202 92-93 0.48317746819762775 2202 94-95 0.2950839629188522 2202 96-97 0.20653199762447016 2202 98-99 0.03413892781805572 2202 100-101 0.22477253499726046 1102 1 0.13407749229264 1102 2 0.11531929021830223 1102 3 0.1085219335733143 1102 4 0.0873650964020527 1102 5 0.13243861270898094 1102 6 0.09555626702329789 1102 7 0.051426120654667784 1102 8 0.05624158492715026 1102 9 0.15300783268044427 1102 10-11 0.13223314404234543 1102 12-13 0.12719043148550924 1102 14-15 0.21505097852148936 1102 16-17 0.24599977865731404 1102 18-19 0.17520901033550018 1102 20-21 0.08198887885186679 1102 22-23 0.08046977233791353 1102 24-25 0.22679578762837593 1102 26-27 0.2301218992102605 1102 28-29 0.14883847202224842 1102 30-31 0.1755811410155701 1102 32-33 0.06431347146338595 1102 34-35 0.1602145529222625 1102 36-37 0.05626610475054861 1102 38-39 0.10687160393775486 1102 40-41 0.2433341700409457 1102 42-43 0.21479593882542503 1102 44-45 0.24284661792879092 1102 46-47 0.38116333011792847 1102 48-49 0.3192009770292259 1102 50-51 0.3577493624670609 1102 52-53 0.34314001102811176 1102 54-55 0.5213715358081643 1102 56-57 0.30656831032143117 1102 58-59 0.32011375310805334 1102 60-61 0.29631863293384697 1102 62-63 0.4235066787180912 1102 64-65 0.2702109844979148 1102 66-67 0.31911052529103046 1102 68-69 0.21884440444366504 1102 70-71 0.13567645248134852 1102 72-73 0.060028433877576504 1102 74-75 -0.020490532803371764 1102 76-77 -0.19028721817841543 1102 78-79 0.14423501489199353 1102 80-81 0.20355719270178696 1102 82-83 -0.05391061484730031 1102 84-85 -0.1373251792386263 1102 86-87 0.0374177128215436 1102 88-89 0.2412428076541353 1102 90-91 0.384716062484177 1102 92-93 0.18016917022421453 1102 94-95 0.2512306366211021 1102 96-97 0.053149650262259485 1102 98-99 -0.001913948371193186 1102 100-101 -0.061246587524912854 2305 1 0.09896489473884884 2305 2 0.09424682158694964 2305 3 0.07071297307362556 2305 4 0.08404790531884032 2305 5 0.10547917479796354 2305 6 0.0608003103246233 2305 7 0.12666716161004388 2305 8 0.10688171214901843 2305 9 0.04756010170309821 2305 10-11 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36.0 11 30.0 12 43.0 13 131.0 14 439.0 15 1243.0 16 2708.0 17 4394.0 18 5441.0 19 6520.0 20 8500.0 21 11191.0 22 14519.0 23 19340.0 24 25323.0 25 32442.0 26 43977.0 27 64182.0 28 98435.0 29 112413.0 30 72651.0 31 64499.0 32 84540.0 33 120093.0 34 183827.0 35 321582.0 36 647618.0 37 979838.0 38 210142.0 39 181.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.63308525249944 23.73996230874884 21.978056025808925 13.648896412942793 2 27.876182802607275 28.64272274886881 30.655475989071203 12.825618459452711 3 34.502173101370055 17.789619044855705 32.33414139778038 15.374066455993859 4 27.44642674359613 17.47468856354509 31.001714586549312 24.077170106309467 5 36.405976339643416 16.32273177861436 31.622761973420282 15.648529908321937 6 31.85188538982706 17.08455279005427 35.28325246843336 15.780309351685313 7 30.299292064122675 26.017719600255205 26.55051284937256 17.132475486249565 8 27.09140481095405 33.50383142434352 22.913999644804502 16.49076411989793 9 34.78113229565657 23.50721439045551 23.927390897748655 17.784262416139267 10-11 26.37403650365375 29.878494398058308 27.15411222504443 16.593356873243515 12-13 21.95879795976215 28.545283121741182 27.11008924207211 22.38582967642455 14-15 21.075225241454824 29.147505293656444 27.957743851754024 21.819525613134708 16-17 24.557757732862715 25.68640017345335 28.583708776930706 21.172133316753232 18-19 19.560048605028662 25.77458286050988 33.017924618842386 21.647443915619068 20-21 24.055870275206363 26.08128173922082 32.45052053358399 17.412327451988823 22-23 19.613949681487842 34.39394050507269 28.364385306129737 17.627724507309726 24-25 20.017896879157966 25.37336180424009 32.63011107671107 21.97863023989088 26-27 19.835149751251553 25.509541335477685 28.189019485055805 26.466289428214957 28-29 23.65661012766313 30.15332393159968 27.89992095784174 18.290144982895455 30-31 18.886611967410015 26.553892627015074 31.977367605979147 22.582127799595767 32-33 18.552587905306282 30.914076805765774 28.426751769042575 22.106583519885366 34-35 19.00844338601429 26.563729055342584 32.746235134956756 21.681592423686375 36-37 23.32590207381243 27.17304557183765 28.34485593060105 21.15619642374887 38-39 23.896375749903946 31.2139874722398 27.426388647534385 17.46324813032187 40-41 23.669681123966036 30.847062394833852 27.295097204848428 18.188159276351684 42-43 19.097349100094448 30.91663270856809 32.075624207245795 17.910393984091666 44-45 18.741433864130183 26.726327971266407 32.48672503024702 22.04551313435639 46-47 23.43978065677016 26.342599597364806 28.03473880448884 22.182880941376197 48-49 19.473757299603783 31.299581781949133 31.34184841504909 17.884812503397992 50-51 23.71788034639348 26.802416151424147 30.97739916001957 18.502304342162805 52-53 19.050062944581985 26.710440892677894 26.95113855078231 27.288357611957814 54-55 23.74304303844618 26.715928268831153 31.00025576361064 18.540772929112023 56-57 19.390848730634545 26.79531445160424 30.867320132100563 22.946516685660654 58-59 19.294394068852064 26.674601739401112 31.514996107413072 22.51600808433375 60-61 23.118583406978676 26.92080396157196 31.55290201985442 18.40771061159494 62-63 19.005116906966617 26.987638459663206 31.178326750854158 22.82891788251602 64-65 23.33299583441293 26.82094353536763 30.99778765471469 18.848272975504756 66-67 19.536248699005274 34.914640234993094 26.548935181560424 19.000175884441212 68-69 19.612681806062476 35.330653895857864 26.481190782667046 18.575473515412614 70-71 18.96171502355107 36.14566212091807 26.390032852052858 18.502590003478005 72-73 18.88456083762781 35.85589375233008 26.341306989979962 18.918238420062146 74-75 19.62765982894806 34.54003677473165 26.271542959935317 19.560760436384978 76-77 19.927977486825082 33.47657888916229 26.710522727740262 19.88492089627237 78-79 19.78459694166118 32.99706138092613 27.223055203910253 19.99528647350244 80-81 19.162099300513773 33.11068884852628 27.230824077428178 20.49638777353177 82-83 18.589555753314542 32.449247557515285 27.48912476129955 21.472071927870616 84-85 18.82382371130914 31.846786756824365 27.913348610462073 21.416040921404417 86-87 18.908248058900355 31.7759726228805 28.45667229581577 20.859107022403375 88-89 19.304629699911146 32.1773647775916 27.95631881992819 20.561686702569066 90-91 19.729660689387725 31.485030864394393 27.55782529438854 21.22748315182935 92-93 19.14720415801291 32.092891804964594 27.601636895410753 21.158267141611738 94-95 18.95734506448529 32.41481947914783 27.578143616018497 21.049691840348387 96-97 19.456500001754247 32.80668258670034 26.907557380536755 20.829260031008662 98-99 19.90628168826293 31.932263551930713 26.590996011844215 21.570458747962142 100-101 19.780413444473226 31.983605888049482 26.279063536274638 21.956917131202655 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 916.0 1 601.5 2 291.0 3 413.0 4 487.0 5 391.5 6 353.0 7 386.0 8 434.0 9 444.0 10 444.5 11 457.0 12 449.5 13 449.5 14 467.0 15 495.5 16 503.0 17 551.5 18 604.5 19 611.0 20 682.5 21 794.0 22 954.0 23 1175.5 24 1515.5 25 2236.0 26 3550.5 27 5787.0 28 8135.5 29 10247.0 30 12678.5 31 15109.5 32 18653.5 33 27968.0 34 45421.5 35 76708.5 36 117382.5 37 161179.0 38 210427.5 39 243553.5 40 254461.5 41 240874.5 42 216755.0 43 210687.0 44 201484.5 45 184001.0 46 174210.0 47 154056.5 48 119640.5 49 92676.0 50 73211.5 51 59274.0 52 49209.0 53 38243.5 54 28509.0 55 20565.0 56 14155.0 57 9197.0 58 6136.0 59 4371.0 60 3224.5 61 2352.0 62 1599.5 63 1068.5 64 792.0 65 587.5 66 397.5 67 304.0 68 231.5 69 166.0 70 117.5 71 85.0 72 59.0 73 35.5 74 28.0 75 20.0 76 13.0 77 10.0 78 7.5 79 7.5 80 9.5 81 6.5 82 3.5 83 1.5 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 1.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17858617524274617 2 0.0 3 0.0 4 0.008545098190511689 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0021362745476279222 12-13 0.0 14-15 0.0 16-17 3.507316421478678E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.002104389852887207 40-41 0.006121861390217329 42-43 0.002646429663479366 44-45 0.001323214831739683 46-47 0.006743612937661277 48-49 0.008210308895734179 50-51 0.004989954726921937 52-53 0.006663901200809489 54-55 0.018907623981244147 56-57 0.021027956181501713 58-59 0.02722952930857083 60-61 0.02480629250827647 62-63 0.0103465834433621 64-65 0.015575673380839402 66-67 0.022941037865944626 68-69 0.026049795603164366 70-71 0.028074473719199786 72-73 0.021809131202649237 74-75 0.031757155961752395 76-77 0.028457090056088367 78-79 0.02054968576039098 80-81 0.009087138001103848 82-83 0.0015304653475543323 84-85 0.0076682690851420186 86-87 0.026655604803237955 88-89 0.02432802208716574 90-91 0.0206931668867242 92-93 0.04278926034203988 94-95 0.04463857263700136 96-97 0.03341516008826959 98-99 0.04042979293122695 100-101 0.040589216404930524 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3136301.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.90939031612847 #Duplication Level Percentage of deduplicated Percentage of total 1 86.22609907478683 32.68778845263258 2 6.1413657723365995 4.656308642752399 3 2.063415499093041 2.346684706184014 4 1.1609443099460255 1.7604276392412923 5 0.737980024892955 1.398818640458662 6 0.5595929822142588 1.2728297268556006 7 0.34263421841154823 0.9092338024997495 8 0.28397294895839237 0.861219308902858 9 0.2528312912120622 0.8626212092419927 >10 1.810902627477211 13.480941331380128 >50 0.20733930843154535 5.535678426179587 >100 0.17864450966189485 14.944841979834395 >500 0.026757029086758054 6.876712237722436 >1k 0.007099201944056255 4.235966081790289 >5k 3.3696123863544006E-4 0.7428078151439121 >10k+ 8.424030839275778E-5 7.427119999179952 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 232570 7.415423455848147 TruSeq Adapter, Index 6 (97% over 36bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6748 0.21515792011034657 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT 5736 0.1828906090327427 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5574 0.17772528848474684 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5202 0.16586418204120076 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 4999 0.15939158900883557 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4496 0.14335358755425578 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3590 0.11446605411916777 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005292859326958732 0.0 0.0 2 0.005675475663847316 0.0 0.0 3 0.006281284863920905 0.0 0.0 4 0.006727670590290919 0.0 0.0 5 0.007939288990438099 0.0 0.0 6 0.008959599222140987 0.0 0.0 7 0.010234987011769597 0.0 0.0 8 0.01189299113828679 0.0 0.0 9 0.01364664934902613 0.0 0.0 10-11 0.02243088275009318 0.0 0.0 12-13 0.030417998782642355 0.0 0.0 14-15 0.039903695468005144 0.0 0.0 16-17 0.050728549332477973 0.0 0.0 18-19 0.06266936751287584 0.0 0.0 20-21 0.07223477593509042 0.0 0.0 22-23 0.08135379863093498 0.0 0.0 24-25 0.0924815570954446 0.0 0.0 26-27 0.10496441508643461 0.0 0.0 28-29 0.1195038358882008 0.0 0.0 30-31 0.2143926874365694 0.0 0.0 32-33 0.2331249455967396 0.0 0.0 34-35 0.24721798067213574 0.0 0.0 36-37 0.261135649926458 0.0 0.0 38-39 0.27631276462303844 0.0 0.0 40-41 0.29161741809858177 0.0 0.0 42-43 0.3088989226480494 0.0 0.0 44-45 0.3271210256923682 0.0 0.0 46-47 0.3464909777473527 0.0 0.0 48-49 0.36743922219200265 0.0 0.0 50-51 0.3899338743315772 0.0 0.0 52-53 0.41383145303974334 0.0 0.0 54-55 0.439897190990278 0.0 0.0 56-57 0.4686731279937736 0.0 0.0 58-59 0.49982447475545233 0.0 0.0 60-61 0.5343555991596469 0.0 0.0 62-63 0.5714056144483581 0.0 0.0 64-65 0.6093324588424389 0.0 0.0 66-67 0.6502245798474062 0.0 0.0 68-69 0.6975733515373683 0.0 0.0 70-71 0.7449540079220713 0.0 0.0 72-73 0.7966072134020299 0.0 0.0 74-75 0.8535851629036881 0.0 0.0 76-77 0.9154414707006757 0.0 0.0 78-79 0.9862095506776933 0.0 0.0 80-81 1.0603414659498562 0.0 0.0 82-83 1.1408662625175325 0.0 0.0 84-85 1.2259346280857608 0.0 0.0 86-87 1.3217003087394992 0.0 0.0 88-89 1.4262023957521934 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 33600 0.0 77.969475 3 CGGAAGA 33500 0.0 77.94702 4 GAAGAGC 33635 0.0 77.78577 6 GATCGGA 33670 0.0 77.65477 1 ATCGGAA 33915 0.0 76.81118 2 GGAAGAG 34615 0.0 75.569824 5 TCGGACG 95 0.0 64.99192 3 GAGCACA 42610 0.0 61.479675 9 AGAGCAC 42620 0.0 61.387245 8 AAGAGCA 43235 0.0 60.404194 7 GTCCGCG 25 0.004987875 57.098515 1 CGGAGCC 50 4.1940257E-7 57.098515 1 CTCGGAA 345 0.0 48.182415 2 CGAGGCG 100 1.2732926E-11 47.582096 1 GCCGTCT 28350 0.0 44.87178 50-51 CGTCTTC 28475 0.0 44.709232 52-53 ATGCCGT 28290 0.0 44.688824 48-49 TCGTATG 28810 0.0 44.555023 44-45 TCTCGTA 28215 0.0 44.42421 42-43 CTGCTTG 28585 0.0 44.398407 58-59 >>END_MODULE