FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_41442-1.34100000035721.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_41442-1.34100000035721.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3136301
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2325707.415423455848147TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67480.21515792011034657No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT57360.1828906090327427TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC55740.17772528848474684No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC52020.16586418204120076No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG49990.15939158900883557No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG44960.14335358755425578No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA35900.11446605411916777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG336000.077.9694753
CGGAAGA335000.077.947024
GAAGAGC336350.077.785776
GATCGGA336700.077.654771
ATCGGAA339150.076.811182
GGAAGAG346150.075.5698245
TCGGACG950.064.991923
GAGCACA426100.061.4796759
AGAGCAC426200.061.3872458
AAGAGCA432350.060.4041947
GTCCGCG250.00498787557.0985151
CGGAGCC504.1940257E-757.0985151
CTCGGAA3450.048.1824152
CGAGGCG1001.2732926E-1147.5820961
GCCGTCT283500.044.8717850-51
CGTCTTC284750.044.70923252-53
ATGCCGT282900.044.68882448-49
TCGTATG288100.044.55502344-45
TCTCGTA282150.044.4242142-43
CTGCTTG285850.044.39840758-59
Produced by FastQC (version 0.11.2)