##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_40825-1.341000000356f9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1854631 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.63815227934829 33.0 33.0 33.0 33.0 33.0 2 32.645281999492084 33.0 33.0 33.0 33.0 33.0 3 32.70272253618105 33.0 33.0 33.0 33.0 33.0 4 36.649754587300656 37.0 37.0 37.0 37.0 37.0 5 36.61251483448729 37.0 37.0 37.0 37.0 37.0 6 36.522253213712055 37.0 37.0 37.0 37.0 37.0 7 36.45062279235061 37.0 37.0 37.0 37.0 37.0 8 36.37601442011915 37.0 37.0 37.0 37.0 37.0 9 36.40658006902721 37.0 37.0 37.0 37.0 37.0 10-11 36.34578980940144 37.0 37.0 37.0 37.0 37.0 12-13 36.334053512531604 37.0 37.0 37.0 37.0 37.0 14-15 38.16291893104342 40.0 37.0 40.0 37.0 40.0 16-17 38.10711214252323 40.0 37.0 40.0 37.0 40.0 18-19 38.00990817041233 40.0 37.0 40.0 33.0 40.0 20-21 37.953651696752615 40.0 37.0 40.0 33.0 40.0 22-23 37.75189592970246 40.0 37.0 40.0 33.0 40.0 24-25 37.71049389339443 40.0 37.0 40.0 33.0 40.0 26-27 37.583619598723416 40.0 37.0 40.0 33.0 40.0 28-29 37.37856074874193 40.0 37.0 40.0 33.0 40.0 30-31 37.274377221129164 40.0 37.0 40.0 33.0 40.0 32-33 37.10798940597887 40.0 37.0 40.0 33.0 40.0 34-35 36.93297292021971 37.0 37.0 40.0 33.0 40.0 36-37 36.81803010949348 37.0 37.0 40.0 33.0 40.0 38-39 36.68130264187324 37.0 37.0 40.0 33.0 40.0 40-41 36.5397477988883 37.0 37.0 40.0 33.0 40.0 42-43 36.19601284568197 37.0 37.0 40.0 33.0 40.0 44-45 35.98853545530081 37.0 37.0 40.0 33.0 40.0 46-47 35.98348242858013 37.0 37.0 40.0 33.0 40.0 48-49 35.683989160107856 37.0 37.0 40.0 30.0 40.0 50-51 35.510251365365946 37.0 33.0 40.0 27.0 40.0 52-53 35.3233875094291 37.0 33.0 40.0 27.0 40.0 54-55 34.919668926055905 37.0 33.0 40.0 27.0 40.0 56-57 34.73370578837515 37.0 33.0 38.5 27.0 40.0 58-59 34.50134231553339 37.0 33.0 37.0 27.0 40.0 60-61 34.21230773129534 37.0 33.0 37.0 27.0 40.0 62-63 33.856208054324554 37.0 33.0 37.0 27.0 40.0 64-65 33.59734173536407 37.0 33.0 37.0 27.0 40.0 66-67 33.13707335852793 37.0 33.0 37.0 27.0 37.0 68-69 32.87589121501797 37.0 33.0 37.0 22.0 37.0 70-71 32.43661731093678 35.0 33.0 37.0 22.0 37.0 72-73 31.949835304165628 33.0 33.0 37.0 22.0 37.0 74-75 31.415565414360053 33.0 33.0 37.0 18.5 37.0 76-77 28.686389637615243 30.0 27.0 33.0 15.0 35.0 78-79 31.116578715658264 33.0 33.0 37.0 15.0 37.0 80-81 31.580623584961106 33.0 33.0 37.0 15.0 37.0 82-83 31.5454403059153 33.0 33.0 37.0 15.0 37.0 84-85 31.43751155890309 33.0 33.0 37.0 15.0 37.0 86-87 31.30048645795309 33.0 33.0 37.0 15.0 37.0 88-89 31.088236420074935 33.0 33.0 37.0 15.0 37.0 90-91 30.836019941433094 33.0 33.0 37.0 15.0 37.0 92-93 30.818377078782788 33.0 33.0 37.0 15.0 37.0 94-95 30.73097910042483 33.0 30.0 37.0 15.0 37.0 96-97 30.53583516074087 33.0 27.0 37.0 15.0 37.0 98-99 30.351033170479734 33.0 27.0 37.0 15.0 37.0 100-101 28.600479286715256 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.21395842379051544 2306 2 0.14842145906865056 2306 3 0.11210277065348606 2306 4 0.15399431128037122 2306 5 0.1977307052250552 2306 6 0.16672975245037946 2306 7 0.2074990290121974 2306 8 0.24172191084388572 2306 9 0.1624062898170422 2306 10-11 0.13999177202740043 2306 12-13 0.19134761882499163 2306 14-15 0.3603792949338356 2306 16-17 0.27830295002016214 2306 18-19 0.33550872590362957 2306 20-21 0.3920436489244068 2306 22-23 0.5355112826267643 2306 24-25 0.4075081398861542 2306 26-27 0.4676426403924765 2306 28-29 0.5407812929233629 2306 30-31 0.5307204089668716 2306 32-33 0.5734380940274946 2306 34-35 0.4916322287840842 2306 36-37 0.6674498546651506 2306 38-39 0.5177861950228717 2306 40-41 0.5454286566782969 2306 42-43 0.3988623440657548 2306 44-45 0.6523233926515886 2306 46-47 0.742206408006922 2306 48-49 0.8058787393729787 2306 50-51 0.6905355959463861 2306 52-53 0.6244466417151031 2306 54-55 0.77975515342996 2306 56-57 0.7456600060968341 2306 58-59 0.8535380295114621 2306 60-61 0.8331842921595367 2306 62-63 0.5839775799799654 2306 64-65 0.5480052337817796 2306 66-67 0.7079171922198171 2306 68-69 0.6804997583873202 2306 70-71 0.6197402130980905 2306 72-73 0.4908456737606244 2306 74-75 0.6186004671974494 2306 76-77 0.18110890393667134 2306 78-79 0.7776736514025337 2306 80-81 0.792089977613859 2306 82-83 0.7080246227840128 2306 84-85 0.637967913084271 2306 86-87 0.906213346749599 2306 88-89 0.9870790502283668 2306 90-91 0.8936706008022917 2306 92-93 0.8470894856219751 2306 94-95 0.7059685310291712 2306 96-97 0.7383746359156085 2306 98-99 0.8918568675760561 2306 100-101 0.7029055801575446 2307 1 0.1765947612946519 2307 2 0.1534270544881835 2307 3 0.1461182208813696 2307 4 0.07665413327552528 2307 5 0.17728211348096323 2307 6 0.15979649432526344 2307 7 0.1975316795054809 2307 8 0.1963230812718635 2307 9 0.19526559439407976 2307 10-11 0.1987712474138803 2307 12-13 0.27154403703332974 2307 14-15 0.30788472312355 2307 16-17 0.42043745341990046 2307 18-19 0.34088358285433884 2307 20-21 0.44487177643850373 2307 22-23 0.4522468750994335 2307 24-25 0.49924929162055065 2307 26-27 0.42197172337642996 2307 28-29 0.5110324630801628 2307 30-31 0.538351286643227 2307 32-33 0.3387773626636772 2307 34-35 0.5788730903521397 2307 36-37 0.6064699511703182 2307 38-39 0.6168752896496414 2307 40-41 0.5272575543535325 2307 42-43 0.6161399871557265 2307 44-45 0.6038378169018941 2307 46-47 0.6637927953184217 2307 48-49 0.7800478331319667 2307 50-51 0.7056787085182776 2307 52-53 0.4933307758533161 2307 54-55 0.46116923074618654 2307 56-57 0.8193378509473277 2307 58-59 0.7407112307965065 2307 60-61 0.6931128172286449 2307 62-63 0.5747446857831093 2307 64-65 0.6391443581483358 2307 66-67 0.6530554144492697 2307 68-69 0.6492939951955989 2307 70-71 0.645774280977129 2307 72-73 0.6765799302025925 2307 74-75 0.6350228879259951 2307 76-77 0.18949276779532553 2307 78-79 0.7796645902578838 2307 80-81 0.9045129389015081 2307 82-83 0.7914601019019791 2307 84-85 0.9143888694219164 2307 86-87 0.7231351066943539 2307 88-89 1.0671240576497567 2307 90-91 0.9207914365536816 2307 92-93 0.8204418650763934 2307 94-95 0.837625181985274 2307 96-97 0.7071397204977394 2307 98-99 0.6315202565381703 2307 100-101 0.48658973454411836 2201 1 0.1562977891196553 2201 2 0.18293059448258475 2201 3 0.11780256958696356 2201 4 0.1088880957020919 2201 5 0.21901315998508863 2201 6 0.13270230249845838 2201 7 0.0647321787630446 2201 8 0.2182181080639225 2201 9 0.12226466904599675 2201 10-11 0.2240218445861899 2201 12-13 0.20964685826899654 2201 14-15 0.3197057220690809 2201 16-17 0.39276050749895575 2201 18-19 0.265907362148063 2201 20-21 0.33755593146658924 2201 22-23 0.4140614181281208 2201 24-25 0.3182088130219185 2201 26-27 0.2805292563554076 2201 28-29 0.27252270388586197 2201 30-31 0.35771796120045707 2201 32-33 0.27238686577327087 2201 34-35 0.42761483215850404 2201 36-37 0.4857455973000029 2201 38-39 0.3920374407611291 2201 40-41 0.36792100740824907 2201 42-43 0.38735434511479383 2201 44-45 0.47569475378455195 2201 46-47 0.6065511933902528 2201 48-49 0.5525598303774402 2201 50-51 0.5805430266658504 2201 52-53 0.5867283096930791 2201 54-55 0.644202220481283 2201 56-57 0.7055839504975623 2201 58-59 0.6130913121410586 2201 60-61 0.41276161535213163 2201 62-63 0.4570023199047881 2201 64-65 0.6038593294195067 2201 66-67 0.5644081442261069 2201 68-69 0.47645963250219125 2201 70-71 0.5247852345160453 2201 72-73 0.43995529872784545 2201 74-75 0.5977778428421736 2201 76-77 0.45106650512558844 2201 78-79 0.705060660756498 2201 80-81 0.8818832287721712 2201 82-83 0.6931063606981631 2201 84-85 0.6377511109162519 2201 86-87 0.7040776705541312 2201 88-89 0.834879120164544 2201 90-91 0.8577072298782618 2201 92-93 0.7799109735142764 2201 94-95 0.6830132901864445 2201 96-97 0.6262634373842673 2201 98-99 0.7319101939157768 2201 100-101 0.4705338693436616 1101 1 0.200911162222269 1101 2 0.21945840914683146 1101 3 0.17598149050464684 1101 4 0.1484296593704002 1101 5 0.2100609852726265 1101 6 0.16773550031659568 1101 7 0.15215500660426784 1101 8 0.13886531229690036 1101 9 0.12532663149671208 1101 10-11 0.15851958713455616 1101 12-13 0.15571930101552311 1101 14-15 0.24049041634270907 1101 16-17 0.2673753106614356 1101 18-19 0.298593831738863 1101 20-21 0.29343981060610247 1101 22-23 0.2807745276911362 1101 24-25 0.4008186722631635 1101 26-27 0.17615511183764454 1101 28-29 0.2542590008480019 1101 30-31 0.46923913630107705 1101 32-33 0.3508174772260091 1101 34-35 0.3019477092346392 1101 36-37 0.5698890935920602 1101 38-39 0.49898119854985623 1101 40-41 0.655953653631876 1101 42-43 0.6133904234820804 1101 44-45 0.6130985822590844 1101 46-47 0.6736344841622852 1101 48-49 0.663102231652239 1101 50-51 0.498202835855011 1101 52-53 0.4782094431176631 1101 54-55 0.7497496269655741 1101 56-57 0.6175970661894041 1101 58-59 0.755316280986186 1101 60-61 0.7375371981015206 1101 62-63 0.46301408725447857 1101 64-65 0.6472179271436715 1101 66-67 0.5354361529699077 1101 68-69 0.6601352949263415 1101 70-71 0.8087448659691958 1101 72-73 0.7909616522794991 1101 74-75 0.6686891995603936 1101 76-77 0.3475307842732711 1101 78-79 0.7763628324045406 1101 80-81 0.976281071424733 1101 82-83 1.0162876309999014 1101 84-85 0.9045528841904762 1101 86-87 0.9596943327944238 1101 88-89 0.8617027529251757 1101 90-91 1.0161304867374419 1101 92-93 0.8865940518202677 1101 94-95 0.8187639694648752 1101 96-97 0.6109115405120136 1101 98-99 0.8151106678784679 1101 100-101 0.7879479125314681 2304 1 0.11759044924120587 2304 2 0.1304528011212014 2304 3 0.12250102680996378 2304 4 0.018625511940207673 2304 5 0.15547490223224258 2304 6 0.09046605503079519 2304 7 0.0935158783111234 2304 8 0.11200882023472047 2304 9 0.1644829953667113 2304 10-11 0.1342256002352471 2304 12-13 0.10073108765966765 2304 14-15 0.29447703265034875 2304 16-17 0.16283959429964767 2304 18-19 0.269142518763303 2304 20-21 0.36646040396152557 2304 22-23 0.3721290509783586 2304 24-25 0.2734133478512746 2304 26-27 0.30147673960303223 2304 28-29 0.3830326773937358 2304 30-31 0.46072364921666775 2304 32-33 0.5073369982339244 2304 34-35 0.49630113416878885 2304 36-37 0.39604947761790044 2304 38-39 0.4244817293695178 2304 40-41 0.5604221761787116 2304 42-43 0.5518463195576828 2304 44-45 0.5074253132360056 2304 46-47 0.49732452984031283 2304 48-49 0.5967132509789721 2304 50-51 0.5624352660300431 2304 52-53 0.45532033088678503 2304 54-55 0.6977252842182295 2304 56-57 0.722352300193684 2304 58-59 0.7239059451471377 2304 60-61 0.849407633740789 2304 62-63 0.8729342195463587 2304 64-65 0.6240700976912876 2304 66-67 0.6220947579085134 2304 68-69 0.6563598974234921 2304 70-71 0.7132214196492868 2304 72-73 0.5874198460240905 2304 74-75 0.5089621969017095 2304 76-77 0.5892330938742241 2304 78-79 0.7945082219221469 2304 80-81 0.8778903193129288 2304 82-83 0.6567108487767008 2304 84-85 0.8874008693703566 2304 86-87 0.9914981941632952 2304 88-89 0.891425403474507 2304 90-91 1.074285069990463 2304 92-93 0.9005167861993293 2304 94-95 0.879844753487049 2304 96-97 0.7325642788989946 2304 98-99 0.7501843095461318 2304 100-101 0.5582578836805787 2202 1 0.17269469536375937 2202 2 0.15299284688779125 2202 3 0.11127549922287727 2202 4 0.1033381994560969 2202 5 0.1639480779250988 2202 6 0.13469437946506702 2202 7 0.1945265571822148 2202 8 0.14631469361381733 2202 9 0.07595642531840241 2202 10-11 0.131241237153624 2202 12-13 0.07551178941405823 2202 14-15 0.19501415444032943 2202 16-17 0.26940265049085355 2202 18-19 0.3056413745985438 2202 20-21 0.19366609935943302 2202 22-23 0.22055264619829273 2202 24-25 0.25833897714189646 2202 26-27 0.22926723032259133 2202 28-29 0.36858759730220214 2202 30-31 0.3601420321304758 2202 32-33 0.43329046886702827 2202 34-35 0.5323931758672416 2202 36-37 0.5025839296901111 2202 38-39 0.4886248770938977 2202 40-41 0.5091000302341371 2202 42-43 0.3501745677143049 2202 44-45 0.389875097115997 2202 46-47 0.43183698852325136 2202 48-49 0.46108840989074196 2202 50-51 0.4006090519441017 2202 52-53 0.39392317858573733 2202 54-55 0.45417392393345324 2202 56-57 0.4834220942440268 2202 58-59 0.6081450755819233 2202 60-61 0.6397489762274304 2202 62-63 0.3377003014177049 2202 64-65 0.4296733267784987 2202 66-67 0.5157192176084919 2202 68-69 0.4831206398592869 2202 70-71 0.5256737461176328 2202 72-73 0.444971333438275 2202 74-75 0.5309810120555305 2202 76-77 0.3266409250463589 2202 78-79 0.7717329867327258 2202 80-81 0.8332075072102079 2202 82-83 0.6341835159518929 2202 84-85 0.7343592980507516 2202 86-87 0.8056000248269122 2202 88-89 0.9307497106191818 2202 90-91 0.9735985711346302 2202 92-93 0.8339422883605323 2202 94-95 0.7819455671153221 2202 96-97 0.7698608718305984 2202 98-99 0.7419781056305474 2202 100-101 0.9604508661367177 1102 1 0.15278608028865648 1102 2 0.11331712697576535 1102 3 0.09662322227199382 1102 4 0.08242707811533023 1102 5 0.10337317127070378 1102 6 0.12862819359222044 1102 7 0.08233962727240396 1102 8 0.045559404029312134 1102 9 0.091959016831197 1102 10-11 0.13559054579939556 1102 12-13 0.14740666265157643 1102 14-15 0.18005839770154353 1102 16-17 0.17160373142447582 1102 18-19 0.2596334977635948 1102 20-21 0.21930332166690647 1102 22-23 0.2393675405770992 1102 24-25 0.2608298983223918 1102 26-27 0.3782749537126975 1102 28-29 0.40377164585895997 1102 30-31 0.2906860793232866 1102 32-33 0.20491766611183948 1102 34-35 0.2246655234027486 1102 36-37 0.18532991028786938 1102 38-39 0.2787890630690413 1102 40-41 0.2439456476791051 1102 42-43 0.21391129047358248 1102 44-45 0.1298619690577567 1102 46-47 0.3253718460752921 1102 48-49 0.13149145832816345 1102 50-51 0.0990711936930353 1102 52-53 0.19877288789302128 1102 54-55 0.3184315066657959 1102 56-57 0.22678304764794888 1102 58-59 0.3771926946295423 1102 60-61 0.23375098805973238 1102 62-63 0.3784816766244319 1102 64-65 0.43889365651439505 1102 66-67 0.4164395558691041 1102 68-69 0.31347458594900246 1102 70-71 0.23512850546497788 1102 72-73 0.2056724058471886 1102 74-75 0.17606054762915946 1102 76-77 0.029558934355346622 1102 78-79 0.4452177189714881 1102 80-81 0.6177019064736733 1102 82-83 0.5123389385219177 1102 84-85 0.43792603802995345 1102 86-87 0.3484546102701067 1102 88-89 0.3187435046280136 1102 90-91 0.5300969599638456 1102 92-93 0.2996993681347959 1102 94-95 0.34403275788135446 1102 96-97 0.27687756321893175 1102 98-99 0.2606753589679691 1102 100-101 0.2402225743187394 2305 1 0.08159628568949984 2305 2 0.07838381299083608 2305 3 0.0768340978310107 2305 4 -0.0011899950875786658 2305 5 0.08471889637393559 2305 6 0.013947376210325046 2305 7 0.025156204452727593 2305 8 0.06632276431848538 2305 9 0.0682389793656597 2305 10-11 0.08770342391767372 2305 12-13 0.06673159042487953 2305 14-15 0.10540452516352161 2305 16-17 0.1501652659283863 2305 18-19 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-1.4946775596274904 1213 66-67 -1.555834140646013 1213 68-69 -1.809335638686619 1213 70-71 -1.824963332992116 1213 72-73 -1.5321976952118561 1213 74-75 -1.6292274551542398 1213 76-77 -1.2510167382138029 1213 78-79 -1.3799554111031362 1213 80-81 -1.6156979881153468 1213 82-83 -1.640192245560332 1213 84-85 -1.5221622407970195 1213 86-87 -1.9860609412945784 1213 88-89 -1.9555947773567013 1213 90-91 -1.7005064432722037 1213 92-93 -1.9296936460336802 1213 94-95 -1.9164764759140027 1213 96-97 -2.1303064593238616 1213 98-99 -2.1014216995144572 1213 100-101 -1.7729694149059156 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 14.0 10 16.0 11 17.0 12 34.0 13 188.0 14 799.0 15 2384.0 16 4875.0 17 6994.0 18 6685.0 19 6398.0 20 6961.0 21 7659.0 22 9301.0 23 11289.0 24 13962.0 25 17228.0 26 21880.0 27 29384.0 28 43447.0 29 58879.0 30 53189.0 31 54386.0 32 72440.0 33 103101.0 34 160442.0 35 272951.0 36 442628.0 37 398826.0 38 48257.0 39 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.16098227350879 23.23760044984678 21.949260437412256 12.65215683923217 2 33.45015800986827 23.46536858275312 30.24763416550246 12.836839241876147 3 38.50744433798422 15.902300781125733 28.90429416956796 16.68596071132209 4 33.45440917559323 15.363187084284855 30.966494741127594 20.21590899899433 5 33.562417537504764 14.617301231350064 35.150334487021944 16.669946744123223 6 29.97572023761061 14.798900697766834 38.53337941617497 16.691999648447588 7 35.88859455061411 19.21514306619484 26.576553503095763 18.319708880095288 8 32.864758542265285 29.051816776490845 21.08570384081793 16.997720840425938 9 34.23796970933841 24.949167785936933 21.83447812529824 18.97838437942642 10-11 27.670537524712408 25.821767625039467 27.57881886751453 18.928875982733597 12-13 25.699343966535658 24.580738702200062 26.189010104975058 23.53090722628922 14-15 25.027673968568408 25.9061236440025 25.938906445540923 23.12729594188817 16-17 26.298684317655248 24.748022109027854 26.918081102022136 22.03521247129477 18-19 23.90418902735908 24.522667851448617 28.743992740334868 22.82915038085743 20-21 25.34533823709406 25.058003451899598 29.014828286597172 20.581830024409168 22-23 23.772869104420234 29.598340586348442 25.746496203287876 20.882294105943448 24-25 23.86423498798413 24.500372311257603 28.33903347889688 23.296359221861383 26-27 22.762802951099168 24.798598750910557 26.5401311635576 25.898467134432675 28-29 24.85370405218073 27.0119500860279 25.934350283156054 22.199995578635317 30-31 22.52620602157518 25.325846489139884 28.371519725487172 23.77642776379776 32-33 22.976996502269184 27.144698864625898 25.856922482154133 24.02138215095078 34-35 23.290535961061796 26.83903159172903 28.208171868150593 21.662260579058586 36-37 22.740965723100715 27.639918668457497 28.508069799329355 21.111045809112433 38-39 22.70166189878062 26.060157053114818 27.276864415987205 23.961316632117356 40-41 25.41883189188694 25.470328168787 24.814411192648382 24.29642874667768 42-43 23.330109800519306 27.358708027459432 27.17200913085665 22.139173041164614 44-45 22.80428466978538 25.19570131110982 27.134004309257136 24.866009709847667 46-47 25.13380345296624 25.115415673021058 25.36979793663226 24.38098293738044 48-49 22.989713764348114 27.097413625521277 27.137344204013168 22.77552840611744 50-51 25.019850048219777 24.891812182011662 26.277042236319236 23.81129553344932 52-53 22.842029061702533 25.002069288124446 24.492606507675237 27.663295142497784 54-55 25.16143714925777 25.02321653340754 27.103887111021947 22.71145920631274 56-57 23.033156288029527 24.96891572717244 26.869593284772385 25.128334700025647 58-59 22.591334532106373 25.14063290470186 27.161836517416905 25.106196045774865 60-61 24.50770661652339 25.12882563390787 27.246099095894706 23.117368653674035 62-63 22.77329406153331 24.998995646362907 27.323164331398413 24.90454596070537 64-65 25.27803767927586 25.582135913952982 26.85833465905567 22.28149174771549 66-67 23.078532732533525 29.410633725147573 24.738660173282746 22.772173369036157 68-69 22.679936142557423 28.35003478720478 25.54214105807036 23.42788801216743 70-71 22.736951564431536 28.88497442413861 25.18718057140638 23.190893440023473 72-73 22.995416384201814 28.968786218196072 25.245993852917653 22.789803544684457 74-75 23.597725498339432 28.34383971154767 25.33083966974689 22.72759512036601 76-77 23.61905003327785 27.626712029539085 25.23523720043191 23.519000736751153 78-79 23.427656463150935 27.784871105324992 25.378017431878664 23.40945499964541 80-81 23.643400715840844 28.02286376780334 25.354840014289852 22.978895502065964 82-83 23.148703326661604 27.741654229070328 25.479620315411694 23.630022128856375 84-85 22.93595321188382 27.851622658113723 25.374308164841185 23.838115965161276 86-87 22.62262650974098 28.102189387344765 25.49605582360638 23.77912827930788 88-89 22.660041932678503 28.251272474769607 25.6682194791449 23.420466113406995 90-91 23.023890503365145 27.690570293175526 25.251472241450024 24.034066962009305 92-93 22.739765413105538 27.511851255682885 24.91272038372823 24.835662947483346 94-95 22.985073177626003 27.990988128561312 24.524255074909227 24.49968361890346 96-97 22.861364648923992 28.689725812384776 24.257410789509738 24.19149874918149 98-99 22.976805378409818 27.551134621261138 24.819828287680306 24.65223171264874 100-101 23.623131806395616 27.752671044192585 24.400967061751597 24.2232300876602 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2955.0 1 1780.5 2 689.5 3 1838.0 4 2249.0 5 1270.0 6 1149.5 7 1410.0 8 1490.5 9 1461.0 10 1306.5 11 1185.0 12 1166.0 13 1126.5 14 1040.0 15 1060.0 16 1127.5 17 1148.5 18 1208.5 19 1249.5 20 1278.0 21 1375.5 22 1462.0 23 1542.0 24 1697.5 25 1911.5 26 2181.0 27 2513.5 28 2898.0 29 3476.0 30 4280.0 31 5244.5 32 6393.5 33 7955.0 34 10736.5 35 15792.0 36 22502.0 37 31381.5 38 40693.5 39 46618.5 40 54203.5 41 59370.5 42 64304.0 43 75241.5 44 80641.5 45 83624.5 46 93571.5 47 99312.5 48 95959.0 49 92784.5 50 89661.0 51 87212.0 52 84878.5 53 81499.5 54 76088.5 55 66979.5 56 57711.0 57 51168.0 58 47556.0 59 41291.0 60 31909.5 61 23631.0 62 16623.0 63 11603.0 64 8765.0 65 7202.0 66 6689.0 67 6297.5 68 5320.0 69 4041.0 70 2771.5 71 1959.5 72 1655.0 73 1359.0 74 908.0 75 536.5 76 408.0 77 351.0 78 266.5 79 210.5 80 162.5 81 127.5 82 112.0 83 75.5 84 52.0 85 42.5 86 32.0 87 27.5 88 23.0 89 17.0 90 9.5 91 5.5 92 4.5 93 4.5 94 2.0 95 1.0 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17976621764652914 2 0.0 3 0.0 4 0.007872185895738829 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0025072372887113394 12-13 0.0 14-15 0.0 16-17 2.1567632591065284E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.001914127392457044 40-41 0.006901642429140891 42-43 0.00218372279984536 44-45 0.001024462548075601 46-47 0.0074677927846563555 48-49 0.008761850740120272 50-51 0.005472786769982816 52-53 0.006928601969879723 54-55 0.018817759435704462 56-57 0.021432834887371126 58-59 0.02766048879804123 60-61 0.025746361405584187 62-63 0.011026452162182128 64-65 0.015960048117388312 66-67 0.02350871952426116 68-69 0.02701345982030927 70-71 0.029062384916460474 72-73 0.02253817605766322 74-75 0.03227057026438143 76-77 0.02938589940532645 78-79 0.020731886828161506 80-81 0.009085365228986252 82-83 0.002102844177628865 84-85 0.007710428651305839 86-87 0.027229136146219923 88-89 0.025261089672285214 90-91 0.021594592131804115 92-93 0.04415972773020617 94-95 0.04604689558192438 96-97 0.034777807553092774 98-99 0.041760328604450156 100-101 0.04140985457484535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1854631.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.99555690554495 #Duplication Level Percentage of deduplicated Percentage of total 1 82.59709897860404 38.816966652819154 2 7.801345684591646 7.3325717012010845 3 2.911285311743526 4.104524236089602 4 1.6296413620655033 3.063436134663166 5 1.0159059153631975 2.387153212806544 6 0.6613666790027322 1.8648777239102508 7 0.438165465935625 1.4414281061895595 8 0.3843139085137871 1.4448836925721664 9 0.30497730694108477 1.2899320544924642 >10 1.9914982564587025 17.952425370078615 >50 0.1614090327778744 5.203882924585934 >100 0.09720003497796913 8.20441112139288 >500 0.004427759364970328 1.3606235137182225 >1k 0.0011372319704885954 1.1861716482471172 >5k 0.0 0.0 >10k+ 2.2707168894186853E-4 4.346711907233229 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 68207 3.677658790346975 TruSeq Adapter, Index 27 (97% over 39bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13258 0.7148591822308589 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 4168 0.22473473159890026 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3890 0.2097452269481099 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 3861 0.20818157358525766 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 2309 0.12449915913192436 TruSeq Adapter, Index 27 (97% over 39bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.00301946856274914 0.0 0.0 2 0.003289063970137456 0.0 0.0 3 0.003558659377525772 0.0 0.0 4 0.003990012029347078 0.0 0.0 5 0.004906636414467353 0.0 0.0 6 0.0057154226366323 0.0 0.0 7 0.0064163706958419225 0.0 0.0 8 0.007440833243917523 0.0 0.0 9 0.008303538547560135 0.0 0.0 10-11 0.01625660306551546 0.0 0.0 12-13 0.02887366813128865 0.0 0.0 14-15 0.043243103345085895 0.0 0.0 16-17 0.062087822321529186 0.0 0.0 18-19 0.08106733900166663 0.0 0.0 20-21 0.08840033408262885 0.0 0.0 22-23 0.0942505544229553 0.0 0.0 24-25 0.10217665940017179 0.0 0.0 26-27 0.1102106025403436 0.0 0.0 28-29 0.11951164409524051 0.0 0.0 30-31 0.17574924607644324 0.0 0.0 32-33 0.18755752492005148 0.0 0.0 34-35 0.19693944509716488 0.0 0.0 36-37 0.2056473767558075 0.0 0.0 38-39 0.21449010611814426 0.0 0.0 40-41 0.22249708971757726 0.0 0.0 42-43 0.2319329289761683 0.0 0.0 44-45 0.2419888376717525 0.0 0.0 46-47 0.2513977173896047 0.0 0.0 48-49 0.26253200771474217 0.0 0.0 50-51 0.2744211651805669 0.0 0.0 52-53 0.28598680815752564 0.0 0.0 54-55 0.29906218541585905 0.0 0.0 56-57 0.313943851903694 0.0 0.0 58-59 0.33025437405068714 0.0 0.0 60-61 0.34891037624195864 0.0 0.0 62-63 0.3684290837368727 0.0 0.0 64-65 0.38875657745395176 0.0 0.0 66-67 0.4123461756004294 0.0 0.0 68-69 0.43739158894680397 0.0 0.0 70-71 0.4640815342782473 0.0 0.0 72-73 0.4965947404092782 0.0 0.0 74-75 0.5352277622880239 0.0 0.0 76-77 0.5801693166996562 0.0 0.0 78-79 0.6307993342071819 0.0 0.0 80-81 0.6843679416552403 0.0 0.0 82-83 0.7406594627179206 0.0 0.0 84-85 0.8076539214539172 0.0 0.0 86-87 0.87958197614512 0.0 0.0 88-89 0.9592204594876286 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 10455 0.0 70.23338 4 ATCGGAA 10455 0.0 70.09709 2 GATCGGA 10610 0.0 69.30639 1 TCGGAAG 10745 0.0 68.60305 3 GAAGAGC 10730 0.0 68.56246 6 GGAAGAG 12840 0.0 57.332565 5 GAGCACA 14095 0.0 51.48645 9 AAGAGCA 14515 0.0 50.258423 7 AGAGCAC 14850 0.0 49.156628 8 TATGCCG 8440 0.0 42.939 48-49 GATCTCG 8155 0.0 42.895023 40-41 GTATGCC 8555 0.0 42.694916 46-47 TACTCGA 8430 0.0 42.594383 36-37 CTCGTAT 8225 0.0 42.386734 44-45 CACATTA 8580 0.0 42.344513 30-31 TCGTATG 8675 0.0 42.268574 44-45 GCCGTCT 8685 0.0 42.193134 50-51 TCTCGTA 8290 0.0 42.16898 42-43 TCACATT 8610 0.0 42.003914 30-31 ACTCGAT 8615 0.0 41.845097 36-37 >>END_MODULE