FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_40825-1.341000000356f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_40825-1.341000000356f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1854631
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT682073.677658790346975TruSeq Adapter, Index 27 (97% over 39bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132580.7148591822308589No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA41680.22473473159890026No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38900.2097452269481099No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC38610.20818157358525766No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT23090.12449915913192436TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA104550.070.233384
ATCGGAA104550.070.097092
GATCGGA106100.069.306391
TCGGAAG107450.068.603053
GAAGAGC107300.068.562466
GGAAGAG128400.057.3325655
GAGCACA140950.051.486459
AAGAGCA145150.050.2584237
AGAGCAC148500.049.1566288
TATGCCG84400.042.93948-49
GATCTCG81550.042.89502340-41
GTATGCC85550.042.69491646-47
TACTCGA84300.042.59438336-37
CTCGTAT82250.042.38673444-45
CACATTA85800.042.34451330-31
TCGTATG86750.042.26857444-45
GCCGTCT86850.042.19313450-51
TCTCGTA82900.042.1689842-43
TCACATT86100.042.00391430-31
ACTCGAT86150.041.84509736-37
Produced by FastQC (version 0.11.2)