##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_40255-1.341000000357f8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7707756 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65806247629012 33.0 33.0 33.0 33.0 33.0 2 32.674037943079675 33.0 33.0 33.0 33.0 33.0 3 32.728859086873015 33.0 33.0 33.0 33.0 33.0 4 36.67578786873897 37.0 37.0 37.0 37.0 37.0 5 36.63635447204089 37.0 37.0 37.0 37.0 37.0 6 36.54070536223513 37.0 37.0 37.0 37.0 37.0 7 36.48193287384811 37.0 37.0 37.0 37.0 37.0 8 36.426201218616676 37.0 37.0 37.0 37.0 37.0 9 36.44436253560699 37.0 37.0 37.0 37.0 37.0 10-11 36.40148351867911 37.0 37.0 37.0 37.0 37.0 12-13 36.38627104957656 37.0 37.0 37.0 37.0 37.0 14-15 38.26105036277744 40.0 37.0 40.0 37.0 40.0 16-17 38.24194305061032 40.0 37.0 40.0 37.0 40.0 18-19 38.21942891030801 40.0 37.0 40.0 37.0 40.0 20-21 38.16049612883438 40.0 37.0 40.0 37.0 40.0 22-23 38.026503239075026 40.0 37.0 40.0 35.0 40.0 24-25 38.02145371493337 40.0 37.0 40.0 35.0 40.0 26-27 37.93331042134702 40.0 37.0 40.0 33.0 40.0 28-29 37.782781720127105 40.0 37.0 40.0 33.0 40.0 30-31 37.73909209113522 40.0 37.0 40.0 33.0 40.0 32-33 37.59850188563312 40.0 37.0 40.0 33.0 40.0 34-35 37.481149117849604 40.0 37.0 40.0 33.0 40.0 36-37 37.34618869616527 40.0 37.0 40.0 33.0 40.0 38-39 37.288294349224344 40.0 37.0 40.0 33.0 40.0 40-41 37.227433380091426 40.0 37.0 40.0 33.0 40.0 42-43 37.03088077775166 38.5 37.0 40.0 33.0 40.0 44-45 36.92178845568023 37.0 37.0 40.0 33.0 40.0 46-47 36.89919167135026 37.0 37.0 40.0 33.0 40.0 48-49 36.65203471671911 37.0 37.0 40.0 33.0 40.0 50-51 36.52279755612399 37.0 37.0 40.0 33.0 40.0 52-53 36.40300244325326 37.0 37.0 40.0 33.0 40.0 54-55 36.11092670811064 37.0 37.0 40.0 33.0 40.0 56-57 35.971147568760614 37.0 37.0 40.0 33.0 40.0 58-59 35.77885535037694 37.0 37.0 40.0 27.0 40.0 60-61 35.48009284414296 37.0 33.0 40.0 27.0 40.0 62-63 35.192063682348014 37.0 33.0 38.5 27.0 40.0 64-65 34.95233534896538 37.0 33.0 37.0 27.0 40.0 66-67 34.52937547581942 37.0 33.0 37.0 27.0 40.0 68-69 34.28062019607263 37.0 33.0 37.0 27.0 40.0 70-71 33.863808091486035 37.0 33.0 37.0 27.0 40.0 72-73 33.45814261634644 37.0 33.0 37.0 27.0 37.0 74-75 32.93308123661413 37.0 33.0 37.0 24.5 37.0 76-77 30.3408199481146 33.0 30.0 35.0 22.0 37.0 78-79 32.56903877081734 35.0 33.0 37.0 27.0 37.0 80-81 32.949374629918225 37.0 33.0 37.0 27.0 37.0 82-83 32.90532250891181 37.0 33.0 37.0 27.0 37.0 84-85 32.76754064347652 37.0 33.0 37.0 27.0 37.0 86-87 32.62253339363623 37.0 33.0 37.0 22.0 37.0 88-89 32.41307152172435 35.0 33.0 37.0 22.0 37.0 90-91 32.17282189524422 33.0 33.0 37.0 22.0 37.0 92-93 32.154045353796874 33.0 33.0 37.0 22.0 37.0 94-95 32.087870646138775 33.0 33.0 37.0 22.0 37.0 96-97 31.91798235439731 33.0 33.0 37.0 22.0 37.0 98-99 31.749373617950543 33.0 33.0 37.0 22.0 37.0 100-101 30.15257890882898 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.17604707309099155 2306 2 0.17036613545909063 2306 3 0.1371702081548989 2306 4 0.15564093828075443 2306 5 0.18599633631107793 2306 6 0.18861792927109633 2306 7 0.15591382282022437 2306 8 0.18563128069626345 2306 9 0.24761964608642018 2306 10-11 0.19450624607206635 2306 12-13 0.15393746828321042 2306 14-15 0.3946108753855171 2306 16-17 0.39856015873425577 2306 18-19 0.46606770302086176 2306 20-21 0.42060689024691555 2306 22-23 0.4580800473386262 2306 24-25 0.4658439142409776 2306 26-27 0.48537081422502837 2306 28-29 0.48207845550935957 2306 30-31 0.5186921692486308 2306 32-33 0.5227370569552505 2306 34-35 0.5446142142903909 2306 36-37 0.5425699550212713 2306 38-39 0.5018612949852255 2306 40-41 0.526679142283264 2306 42-43 0.537353507034851 2306 44-45 0.6178058602495824 2306 46-47 0.6292984378073214 2306 48-49 0.6779577736004043 2306 50-51 0.6321976439470731 2306 52-53 0.5075736696275754 2306 54-55 0.6815892857110839 2306 56-57 0.6860176019342816 2306 58-59 0.7238478671864286 2306 60-61 0.6155312911804458 2306 62-63 0.5674175771582881 2306 64-65 0.5051721168188266 2306 66-67 0.5718669597625095 2306 68-69 0.5383984597480023 2306 70-71 0.5978233126143948 2306 72-73 0.5524379914806516 2306 74-75 0.6244174961725619 2306 76-77 0.2232042081546659 2306 78-79 0.7473193409027203 2306 80-81 0.7266458020232633 2306 82-83 0.6990769292055248 2306 84-85 0.7918272524954588 2306 86-87 0.6959448141878823 2306 88-89 0.7503637759159218 2306 90-91 0.8421008746569285 2306 92-93 0.7330880390593251 2306 94-95 0.6933779694348701 2306 96-97 0.6573311676521314 2306 98-99 0.5474204708382757 2306 100-101 0.48852397383559776 2307 1 0.14821406733618403 2307 2 0.15655452086427601 2307 3 0.1088928191169316 2307 4 0.10603398264694874 2307 5 0.161406407780035 2307 6 0.19065041189746523 2307 7 0.11610819604833722 2307 8 0.17613093849221428 2307 9 0.19026568278518852 2307 10-11 0.19052436377364046 2307 12-13 0.20737297088107454 2307 14-15 0.3694155332163689 2307 16-17 0.37585012539138773 2307 18-19 0.3674117222946478 2307 20-21 0.34603700533318005 2307 22-23 0.3363519850445229 2307 24-25 0.35664015441736296 2307 26-27 0.3900529267757591 2307 28-29 0.4631573140137206 2307 30-31 0.46005533134033527 2307 32-33 0.3757266970975266 2307 34-35 0.46959155303650135 2307 36-37 0.409275562148423 2307 38-39 0.3238781282997749 2307 40-41 0.4245965173738 2307 42-43 0.43289115961812996 2307 44-45 0.45565838110841383 2307 46-47 0.5127526477813333 2307 48-49 0.5205714180851331 2307 50-51 0.3181863244554748 2307 52-53 0.27654832724902434 2307 54-55 0.5811053802704222 2307 56-57 0.5818821350027648 2307 58-59 0.5565540646529641 2307 60-61 0.474228442759717 2307 62-63 0.3843654361642379 2307 64-65 0.4436069662540376 2307 66-67 0.3559684489401249 2307 68-69 0.42164317569537246 2307 70-71 0.4731118758859054 2307 72-73 0.3531822645072893 2307 74-75 0.33501073073706067 2307 76-77 0.1052462073442797 2307 78-79 0.5110264827128077 2307 80-81 0.5173806316142944 2307 82-83 0.411348176852556 2307 84-85 0.380900157081534 2307 86-87 0.35926825202390944 2307 88-89 0.5363557637873768 2307 90-91 0.6410626119565705 2307 92-93 0.5207499461147762 2307 94-95 0.5319307704057437 2307 96-97 0.448306022053373 2307 98-99 0.3886272395312744 2307 100-101 0.4302238055423402 2201 1 0.10116795406140966 2201 2 0.1354536754822533 2201 3 0.11856852427676756 2201 4 0.09735070373199761 2201 5 0.13547806891828174 2201 6 0.14602695296640889 2201 7 0.17207520582836366 2201 8 0.09559373205735255 2201 9 0.17484244937082138 2201 10-11 0.1658683760456583 2201 12-13 0.1501314709200372 2201 14-15 0.25468020211477693 2201 16-17 0.26424110350129126 2201 18-19 0.24044478432549 2201 20-21 0.2957430391270677 2201 22-23 0.3108138847934967 2201 24-25 0.27769517835176316 2201 26-27 0.3418765867772535 2201 28-29 0.35026069263883386 2201 30-31 0.3616865229271511 2201 32-33 0.31853465496440947 2201 34-35 0.34167628375336534 2201 36-37 0.4195209414244161 2201 38-39 0.2463042967925233 2201 40-41 0.36956914520861517 2201 42-43 0.3520054107147743 2201 44-45 0.2879728454986221 2201 46-47 0.41257819320017575 2201 48-49 0.5869390714128073 2201 50-51 0.46926396117496694 2201 52-53 0.4276884394267668 2201 54-55 0.5036080960964711 2201 56-57 0.48870691800406263 2201 58-59 0.5287891398328313 2201 60-61 0.42729615735581206 2201 62-63 0.4676066427702281 2201 64-65 0.4564508142777868 2201 66-67 0.4521970030817002 2201 68-69 0.36823924871021774 2201 70-71 0.515358807499041 2201 72-73 0.32933527972355847 2201 74-75 0.40898448462745307 2201 76-77 0.2584156947521663 2201 78-79 0.4820775188520088 2201 80-81 0.40236974539174497 2201 82-83 0.3766964977768694 2201 84-85 0.3400290453728445 2201 86-87 0.49870497977056516 2201 88-89 0.6165700810416936 2201 90-91 0.710941755787637 2201 92-93 0.4916420357652953 2201 94-95 0.3068632605470256 2201 96-97 0.38417231326631907 2201 98-99 0.31835069128125326 2201 100-101 0.45003786122174105 1101 1 0.1561312394589649 1101 2 0.14937712646550239 1101 3 0.1186137503263538 1101 4 0.10339104665879972 1101 5 0.16427833713232332 1101 6 0.11638994672328096 1101 7 0.11126774409130036 1101 8 0.12239813430266366 1101 9 0.09047591917254749 1101 10-11 0.17280400958559738 1101 12-13 0.1641644800444979 1101 14-15 0.2866129494079104 1101 16-17 0.19848154364165538 1101 18-19 0.26993021877402157 1101 20-21 0.26555423018959345 1101 22-23 0.26526116677664646 1101 24-25 0.3230569481716543 1101 26-27 0.2639779568151397 1101 28-29 0.268142236580978 1101 30-31 0.31499346037029596 1101 32-33 0.284755621530941 1101 34-35 0.2466644476865767 1101 36-37 0.3408546750213759 1101 38-39 0.3316470307100303 1101 40-41 0.3687411465805468 1101 42-43 0.29178959099613877 1101 44-45 0.32429563380241433 1101 46-47 0.356318530585952 1101 48-49 0.3718159237134202 1101 50-51 0.3143210723891059 1101 52-53 0.355417240242069 1101 54-55 0.40277767063633974 1101 56-57 0.36246809388135404 1101 58-59 0.3943910117199394 1101 60-61 0.40625400833289405 1101 62-63 0.3532292619673072 1101 64-65 0.3274249488771872 1101 66-67 0.24631410965259448 1101 68-69 0.42158632006399444 1101 70-71 0.46902922266266955 1101 72-73 0.37945881669802617 1101 74-75 0.4044056978050108 1101 76-77 0.06565332159096471 1101 78-79 0.5098154097666168 1101 80-81 0.46714931399078097 1101 82-83 0.5236764034670855 1101 84-85 0.48048568258319335 1101 86-87 0.5219915416439136 1101 88-89 0.5508804504181555 1101 90-91 0.677892974358933 1101 92-93 0.5600100613410177 1101 94-95 0.38393948902555053 1101 96-97 0.3779888854431803 1101 98-99 0.45375399079090073 1101 100-101 0.5221615286559675 2304 1 0.1561105791099351 2304 2 0.1314375825650984 2304 3 0.12182610383093362 2304 4 0.07788335229447085 2304 5 0.14042036654905132 2304 6 0.12877887921856512 2304 7 0.127547539361494 2304 8 0.03401107477488097 2304 9 0.07006550439237458 2304 10-11 0.10411200025291834 2304 12-13 0.15495525668458043 2304 14-15 0.2719417734928484 2304 16-17 0.19766600689634828 2304 18-19 0.20533127393264294 2304 20-21 0.24906462551290076 2304 22-23 0.26096180412043424 2304 24-25 0.30093916672777254 2304 26-27 0.2511086584830835 2304 28-29 0.2412499553407983 2304 30-31 0.24158800359046495 2304 32-33 0.25640676024057996 2304 34-35 0.3678266192897581 2304 36-37 0.35609474840429556 2304 38-39 0.38932236691204736 2304 40-41 0.3033706191231076 2304 42-43 0.3386364489832303 2304 44-45 0.36640641210041025 2304 46-47 0.393553123022528 2304 48-49 0.39994079069015953 2304 50-51 0.32723286183307465 2304 52-53 0.3832515010902213 2304 54-55 0.49224488221716456 2304 56-57 0.43247812916245465 2304 58-59 0.4225984183176692 2304 60-61 0.3673067950921123 2304 62-63 0.33510325332817814 2304 64-65 0.34632704368137013 2304 66-67 0.385845392486992 2304 68-69 0.4095213632142318 2304 70-71 0.4778879961244158 2304 72-73 0.24822431784367183 2304 74-75 0.35587151013061913 2304 76-77 0.3288684199704832 2304 78-79 0.5312494659653595 2304 80-81 0.572956177333289 2304 82-83 0.443133063258081 2304 84-85 0.4650578213850167 2304 86-87 0.4871189079885596 2304 88-89 0.553519670065576 2304 90-91 0.6868807732269815 2304 92-93 0.4536581540740343 2304 94-95 0.3923518513743858 2304 96-97 0.4339953005324446 2304 98-99 0.31361675162366964 2304 100-101 0.36346875168446147 2202 1 0.13457706442661532 2202 2 0.1161894861350774 2202 3 0.10135166916840177 2202 4 0.07132004112165191 2202 5 0.1307447983633594 2202 6 0.07564262899915519 2202 7 0.07505734209158277 2202 8 0.13324079388726773 2202 9 0.09418279054634837 2202 10-11 0.11692136672983366 2202 12-13 0.10253931251401127 2202 14-15 0.14172625224976088 2202 16-17 0.14797447244028916 2202 18-19 0.15103567651571126 2202 20-21 0.24549337356903322 2202 22-23 0.18192737529431469 2202 24-25 0.1841376741384977 2202 26-27 0.09470004358473005 2202 28-29 0.11851317428125441 2202 30-31 0.1663450599176528 2202 32-33 0.23357524158902976 2202 34-35 0.3150549717187019 2202 36-37 0.3006444619898829 2202 38-39 0.255858527499349 2202 40-41 0.3120422524970863 2202 42-43 0.36103723476828264 2202 44-45 0.29723363612325926 2202 46-47 0.30415751312347084 2202 48-49 0.4863481536682812 2202 50-51 0.3682712914273054 2202 52-53 0.4197854512341408 2202 54-55 0.38889097586218924 2202 56-57 0.41172157212015037 2202 58-59 0.4338390026700978 2202 60-61 0.4880138755608314 2202 62-63 0.4115294841022674 2202 64-65 0.3405137954174364 2202 66-67 0.4212976321009094 2202 68-69 0.37602655256495865 2202 70-71 0.4591463771185502 2202 72-73 0.23129143620278825 2202 74-75 0.3423540624831176 2202 76-77 0.16542808986771718 2202 78-79 0.4643485938066192 2202 80-81 0.5674640088336247 2202 82-83 0.48047594112744463 2202 84-85 0.4722527937549401 2202 86-87 0.4658578980082879 2202 88-89 0.5338692848973281 2202 90-91 0.6931122745527958 2202 92-93 0.5398122708776825 2202 94-95 0.43699218201481216 2202 96-97 0.40333653417306436 2202 98-99 0.2873041786133399 2202 100-101 0.3403434651530155 1102 1 0.10206061836219504 1102 2 0.12555818683434694 1102 3 0.08287231251193106 1102 4 0.050687074263592535 1102 5 0.12736234548300018 1102 6 0.07800267928229232 1102 7 0.11508911615518969 1102 8 0.142463256369048 1102 9 0.11935810382008327 1102 10-11 0.03736277031314472 1102 12-13 0.08925549678608746 1102 14-15 0.16191524108013056 1102 16-17 0.16217828542225732 1102 18-19 0.15148771576166098 1102 20-21 0.14022159195361183 1102 22-23 0.11292551233820092 1102 24-25 0.16031848932233572 1102 26-27 0.1422785972623899 1102 28-29 0.2157206396382847 1102 30-31 0.16141668620026195 1102 32-33 0.17841516573296445 1102 34-35 0.11890849775345913 1102 36-37 0.21015074796065392 1102 38-39 0.11293266673811075 1102 40-41 0.1550627939819833 1102 42-43 0.14530137867955517 1102 44-45 0.15075918849144188 1102 46-47 0.2371214296955202 1102 48-49 0.24795599709643312 1102 50-51 0.13566911559301076 1102 52-53 0.10332988271822074 1102 54-55 0.2531844934806955 1102 56-57 0.3568489129982453 1102 58-59 0.20074707566717365 1102 60-61 0.08582992001793599 1102 62-63 0.21366004264994132 1102 64-65 0.24663376941400372 1102 66-67 0.16602146827002429 1102 68-69 0.06129992241189797 1102 70-71 0.07012760232682069 1102 72-73 0.1423658863987569 1102 74-75 0.10006389953201733 1102 76-77 -0.11493959130184095 1102 78-79 0.2809162046806435 1102 80-81 0.3406816895550975 1102 82-83 0.22046500145395953 1102 84-85 0.25237787727751737 1102 86-87 0.3211696105253452 1102 88-89 0.3693061495430783 1102 90-91 0.38819016543109086 1102 92-93 0.3773966569325111 1102 94-95 0.2763978459725607 1102 96-97 0.16217556352177098 1102 98-99 0.12838480721856627 1102 100-101 0.1921463609055536 2305 1 0.047504154749788086 2305 2 0.08355711364109197 2305 3 0.06650194821639133 2305 4 0.0390426071930392 2305 5 0.0773087624001434 2305 6 0.01504006558867843 2305 7 0.018992610473944183 2305 8 0.07159154612637764 2305 9 0.0495740475127846 2305 10-11 0.03775194558559036 2305 12-13 0.06311379084004187 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-1.1289715461167091 1213 56-57 -1.2593017092221288 1213 58-59 -1.3068724682850288 1213 60-61 -1.398812158230875 1213 62-63 -1.284526908722846 1213 64-65 -1.3088928780153921 1213 66-67 -1.4020268186561538 1213 68-69 -1.6051095154341866 1213 70-71 -1.4428816084072693 1213 72-73 -1.2962755373233747 1213 74-75 -1.276769526960166 1213 76-77 -1.0185675695899619 1213 78-79 -1.2791015233009446 1213 80-81 -1.3457894048010814 1213 82-83 -1.491462797533302 1213 84-85 -1.5573313063916032 1213 86-87 -1.6961529322085092 1213 88-89 -1.7535174609969886 1213 90-91 -1.5553446913406717 1213 92-93 -1.7060630059740767 1213 94-95 -1.6660430770902437 1213 96-97 -1.813981104791356 1213 98-99 -1.7555707059085535 1213 100-101 -1.6881946820685805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 9.0 9 41.0 10 54.0 11 49.0 12 91.0 13 312.0 14 1036.0 15 3018.0 16 6719.0 17 10692.0 18 12511.0 19 14680.0 20 17835.0 21 22175.0 22 27562.0 23 34756.0 24 42321.0 25 53903.0 26 68632.0 27 93356.0 28 135125.0 29 179599.0 30 167073.0 31 175030.0 32 232979.0 33 330333.0 34 511715.0 35 897104.0 36 1711086.0 37 2400117.0 38 557546.0 39 297.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.177947344978215 27.38840704671595 20.345486275155586 15.088159333150248 2 31.621421331967436 25.551366701281154 28.29090593941998 14.536306027331431 3 35.01698808317233 19.99265415251858 26.989723078935036 18.000634685374056 4 30.977259460238667 19.696971859485437 28.27847874283791 21.047289937437988 5 31.750680742877694 18.3382945697814 31.175999862995145 18.735024824345764 6 29.027800049716156 18.838855303670744 33.30475173318927 18.82859291342383 7 31.90845688420858 21.90048569259328 25.890894833723333 20.30016258947481 8 29.933290571211646 28.25313878643797 22.166918620672476 19.646652021677905 9 29.376721318111265 25.56715599196446 24.691739074251963 20.364383615672317 10-11 24.458716535474444 28.689166974318216 28.952031463557937 17.900085026649403 12-13 22.707049885855234 27.943132865129616 28.603513136637954 20.746304112377196 14-15 22.758809438181487 28.51848839013586 28.47741288125882 20.245289290423827 16-17 23.63741110948864 27.558554393438843 28.957760761230954 19.84627373584156 18-19 21.4399690389784 27.342672757155263 30.750220946278006 20.46713725758833 20-21 22.68339189771965 27.846554820884318 30.383732956777564 19.086320324618473 22-23 21.319337301284577 30.52677718391708 28.772148469671325 19.38173704512701 24-25 21.40920781612703 26.94902381445391 30.636089154872053 21.00567921454701 26-27 21.129463620799623 27.18709569944871 29.178946505312307 22.50449417443936 28-29 22.29936962197558 29.311935925319897 28.574801797047026 19.813892655657497 30-31 20.232419137294954 28.785900851038875 29.938266078998872 21.043413932667303 32-33 20.107006500984205 30.130669678697664 28.714511720402154 21.04781209991598 34-35 22.040889721989124 29.911481370193865 28.830226333059844 19.217402574757166 36-37 21.66435341232909 30.498714541560474 29.067558703207524 18.769373342902913 38-39 20.400858765286497 28.677596819493544 31.26278652404079 19.658757891179167 40-41 20.601571647724683 28.094576783206332 28.140098788655347 23.163752780413642 42-43 20.902319671744575 29.606685510132973 29.76129396501566 19.729700853106788 44-45 20.308737403181425 28.197581091669893 29.966771326748663 21.52691017840002 46-47 21.946846185420032 28.090738807363014 28.660005318404608 21.30240968881235 48-49 20.893976707131625 29.681915848803957 29.390229503261935 20.033877940802483 50-51 22.050248500953217 28.372230041994523 28.834083518974087 20.74343793807817 52-53 20.568029091131788 28.19306576936184 27.837295858037685 23.401609281468687 54-55 22.41230797499393 28.02608077974765 29.393600394898296 20.168010850360123 56-57 20.959533582449076 27.646274070271872 29.42976086406841 21.964431483210642 58-59 20.699609609913587 27.86205818969153 29.739433240076796 21.698898960318083 60-61 21.79831070271759 28.010458685486554 29.51214278535044 20.679087826445414 62-63 20.19709061164835 28.160325432546784 29.558265359718654 22.084318596086213 64-65 21.99488109401255 28.23048299497199 29.533598748956972 20.241037162058486 66-67 21.11654271450688 30.63772315606293 27.938632974864813 20.30710115456538 68-69 20.9660463900926 30.41009188616884 28.08335232907225 20.54050939466631 70-71 20.738109530589767 31.082837546303722 27.948735901240536 20.23031702186598 72-73 20.81445791200088 31.304998475864743 27.77077602686423 20.109767585270145 74-75 21.606856874394893 30.57744320113792 27.465023892769352 20.35067603169783 76-77 21.689185480001708 29.59374512928002 27.738287139170897 20.978782251547372 78-79 21.370824710595564 29.538318695090364 28.268439628075193 20.82241696623888 80-81 20.882740460779186 30.077479458474855 28.153970985247785 20.885809095498185 82-83 20.18918041583805 30.036849267010705 28.356582632035636 21.417387685115603 84-85 20.418239396424692 29.821667982978383 28.486665807722993 21.27342681287393 86-87 20.2831989307577 30.034623325099325 28.930568874617673 20.751608869525302 88-89 20.321508536720756 30.41080038622863 28.78556972389039 20.48212135316022 90-91 20.82463838735652 29.43900972021534 28.53135922752156 21.20499266490659 92-93 20.452656624157964 29.949997293748247 28.06458998728386 21.53275609480993 94-95 20.251550538877403 30.350227153637267 27.704410691387892 21.693811616097438 96-97 20.550801964488375 30.690364531436636 27.41400009772735 21.344833406347643 98-99 20.703813332982026 29.94784080207819 27.731160878154963 21.617184986784817 100-101 20.931173253560996 30.10115915287922 27.3184874682527 21.64918012530709 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3154.0 1 1966.5 2 933.0 3 1484.5 4 1567.5 5 1122.5 6 1094.5 7 1213.0 8 1276.0 9 1300.0 10 1241.0 11 1224.5 12 1240.0 13 1273.0 14 1317.5 15 1390.0 16 1492.5 17 1675.0 18 1923.5 19 2297.0 20 2868.0 21 3606.5 22 4690.0 23 6487.0 24 8913.0 25 11900.5 26 15935.5 27 21113.0 28 27466.0 29 34654.0 30 42830.5 31 52744.0 32 68028.5 33 91375.0 34 123144.0 35 174102.0 36 245768.5 37 332921.5 38 417266.0 39 458747.5 40 484854.5 41 481397.0 42 454025.0 43 463990.0 44 462391.5 45 447504.5 46 458284.5 47 426892.0 48 339380.5 49 267667.0 50 223346.5 51 196813.0 52 177219.5 53 151481.5 54 118663.0 55 86958.5 56 65366.5 57 54131.0 58 46214.0 59 36093.0 60 26138.5 61 19153.0 62 13850.5 63 9645.5 64 6802.0 65 4796.0 66 3453.0 67 2549.5 68 1852.0 69 1208.5 70 767.5 71 524.0 72 348.5 73 226.5 74 164.0 75 126.0 76 93.5 77 58.0 78 43.0 79 32.5 80 21.0 81 12.5 82 9.5 83 9.5 84 10.0 85 8.5 86 5.0 87 2.5 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17889772328029066 2 0.0 3 0.0 4 0.008861204220787476 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0022380054583980087 12-13 0.0 14-15 0.0 16-17 3.8273136824777535E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0023158491265162003 40-41 0.0064156156474076236 42-43 0.0028737287480299066 44-45 0.0013687511644115357 46-47 0.0072329741626486355 48-49 0.00877038660798292 50-51 0.005727996579030265 52-53 0.006843755822057677 54-55 0.01904575079958421 56-57 0.02204921899447777 58-59 0.027634502181958016 60-61 0.02604519395787827 62-63 0.010950009315292285 64-65 0.015912543157827 66-67 0.023690422997302975 68-69 0.02699229191998294 70-71 0.029145966737919575 72-73 0.02266548136708012 74-75 0.032382965937167704 76-77 0.029489776272108248 78-79 0.021128068921745836 80-81 0.009672075763685306 82-83 0.0018163522560911375 84-85 0.007978975982114639 86-87 0.027407458149946627 88-89 0.025396496723560008 90-91 0.021419982677189055 92-93 0.043761115427110045 94-95 0.045395832457592065 96-97 0.034322570667779315 98-99 0.041133891628121075 100-101 0.0410495609876597 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7707756.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.330472921065116 #Duplication Level Percentage of deduplicated Percentage of total 1 88.40303156195081 37.421421336732244 2 5.956485175058111 5.042816688150464 3 1.6810473606796525 2.1347858934083406 4 0.8091732110843944 1.3701073880103707 5 0.6528289911541495 1.3817279966068492 6 0.3061980080776766 0.77769038936497 7 0.22613946469651944 0.6700813340704124 8 0.21715167248683304 0.735370639357431 9 0.17609820246958544 0.6708888171978326 >10 1.2007264931530874 10.594201937831702 >50 0.17545061074109505 5.157923204680075 >100 0.16033427054447028 14.053111418928093 >500 0.02108556052854095 6.335671286965934 >1k 0.01391181446204171 10.021447049696588 >5k 2.149152863930325E-4 0.6105991333793205 >10k+ 1.2268762659448993E-4 3.0221554856193933 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 194059 2.5177107318913574 TruSeq Adapter, Index 7 (97% over 38bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15939 0.206791704355976 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 12430 0.16126613245152024 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10340 0.13415058805701685 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 9834 0.12758577204571603 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 9761 0.12663867408361135 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0022574663754275563 0.0 0.0 2 0.0024520755457230355 0.0 0.0 3 0.0027375023288230714 0.0 1.2973944686365266E-5 4 0.002984007277864011 0.0 1.2973944686365266E-5 5 0.003541886899377718 0.0 1.2973944686365266E-5 6 0.00413868835495052 0.0 1.2973944686365266E-5 7 0.004748463755209688 0.0 1.2973944686365266E-5 8 0.005500952547018873 0.0 1.2973944686365266E-5 9 0.006344258951632615 0.0 2.5947889372730532E-5 10-11 0.010794321979055901 0.0 2.5947889372730532E-5 12-13 0.014718940246681396 0.0 2.5947889372730532E-5 14-15 0.01958416950406837 0.0 2.5947889372730532E-5 16-17 0.025986811206789626 0.0 2.5947889372730532E-5 18-19 0.03295381950336777 0.0 2.5947889372730532E-5 20-21 0.038000683986363866 0.0 2.5947889372730532E-5 22-23 0.043384871031205446 0.0 2.5947889372730532E-5 24-25 0.049566955674258506 0.0 2.5947889372730532E-5 26-27 0.05655991186020938 0.0 2.5947889372730532E-5 28-29 0.06445455720186265 0.0 2.5947889372730532E-5 30-31 0.11236084795626639 0.0 3.89218340590958E-5 32-33 0.12373899744620873 0.0 3.89218340590958E-5 34-35 0.13266507139042805 0.0 3.89218340590958E-5 36-37 0.14233714715411333 0.0 3.89218340590958E-5 38-39 0.15313795610551242 0.0 3.89218340590958E-5 40-41 0.1647626105444957 0.0 3.89218340590958E-5 42-43 0.1775743809222814 0.0 3.89218340590958E-5 44-45 0.1909440309215808 0.0 3.89218340590958E-5 46-47 0.2049558911828553 0.0 3.89218340590958E-5 48-49 0.22081005158959366 0.0 3.89218340590958E-5 50-51 0.23813026774589127 0.0 3.89218340590958E-5 52-53 0.25596944168964353 0.0 3.89218340590958E-5 54-55 0.27600121228539154 0.0 3.89218340590958E-5 56-57 0.29730442946040325 0.0 3.89218340590958E-5 58-59 0.32059266017242893 0.0 3.89218340590958E-5 60-61 0.3456129125000843 0.0 3.89218340590958E-5 62-63 0.3725987174477241 0.0 3.89218340590958E-5 64-65 0.40172522326861415 0.0 3.89218340590958E-5 66-67 0.43380978847799545 0.0 3.89218340590958E-5 68-69 0.4686772648226021 0.0 3.89218340590958E-5 70-71 0.5075017942965501 0.0 3.89218340590958E-5 72-73 0.550192559287035 0.0 3.89218340590958E-5 74-75 0.5986113208565502 0.0 4.540880640227843E-5 76-77 0.6518758507664228 0.0 5.1895778745461064E-5 78-79 0.7126328337326713 0.0 5.1895778745461064E-5 80-81 0.7786637252139273 0.0 6.486972343182633E-5 82-83 0.853490951192539 0.0 7.78436681181916E-5 84-85 0.9357781953658107 0.0 8.433064046137423E-5 86-87 1.0259471109360494 0.0 9.081761280455686E-5 88-89 1.1250485874228504 0.0 1.0379155749092213E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 33355 0.0 63.36182 4 TCGGAAG 33590 0.0 63.07407 3 GAAGAGC 34360 0.0 61.76796 6 GATCGGA 34360 0.0 61.623283 1 ATCGGAA 35065 0.0 60.190617 2 GGAAGAG 38920 0.0 54.48221 5 GCCGTCT 24200 0.0 43.369812 50-51 ATGCCGT 24175 0.0 43.17649 48-49 TGCCGTC 24195 0.0 43.103916 50-51 TATGCCG 24125 0.0 43.08878 48-49 TCGTATG 24655 0.0 42.77415 44-45 CGTATGC 24570 0.0 42.535942 46-47 CTCGTAT 24450 0.0 42.39465 44-45 CATCTCG 24310 0.0 42.373676 40-41 TCTCGTA 24620 0.0 42.322933 42-43 CGTCTTC 24950 0.0 42.106373 52-53 ATCTCGT 24485 0.0 41.867702 42-43 GTATGCC 25305 0.0 41.600758 46-47 GTCACGA 25690 0.0 40.33505 28-29 CGAGATT 25790 0.0 40.253094 32-33 >>END_MODULE