FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_40255-1.341000000357f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_40255-1.341000000357f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7707756
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1940592.5177107318913574TruSeq Adapter, Index 7 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159390.206791704355976No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA124300.16126613245152024No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC103400.13415058805701685No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG98340.12758577204571603No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC97610.12663867408361135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA333550.063.361824
TCGGAAG335900.063.074073
GAAGAGC343600.061.767966
GATCGGA343600.061.6232831
ATCGGAA350650.060.1906172
GGAAGAG389200.054.482215
GCCGTCT242000.043.36981250-51
ATGCCGT241750.043.1764948-49
TGCCGTC241950.043.10391650-51
TATGCCG241250.043.0887848-49
TCGTATG246550.042.7741544-45
CGTATGC245700.042.53594246-47
CTCGTAT244500.042.3946544-45
CATCTCG243100.042.37367640-41
TCTCGTA246200.042.32293342-43
CGTCTTC249500.042.10637352-53
ATCTCGT244850.041.86770242-43
GTATGCC253050.041.60075846-47
GTCACGA256900.040.3350528-29
CGAGATT257900.040.25309432-33
Produced by FastQC (version 0.11.2)