##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_35011-1.341000000358a7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6120387 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.671652952664594 33.0 33.0 33.0 33.0 33.0 2 32.68815795471757 33.0 33.0 33.0 33.0 33.0 3 32.74734163052108 33.0 33.0 33.0 33.0 33.0 4 36.69160789995796 37.0 37.0 37.0 37.0 37.0 5 36.6518924375207 37.0 37.0 37.0 37.0 37.0 6 36.55791602720547 37.0 37.0 37.0 37.0 37.0 7 36.500012009044525 37.0 37.0 37.0 37.0 37.0 8 36.449500497272474 37.0 37.0 37.0 37.0 37.0 9 36.47588216235346 37.0 37.0 37.0 37.0 37.0 10-11 36.4273885785327 37.0 37.0 37.0 37.0 37.0 12-13 36.41138918176252 37.0 37.0 37.0 37.0 37.0 14-15 38.29269946491946 40.0 37.0 40.0 37.0 40.0 16-17 38.30298476223807 40.0 37.0 40.0 37.0 40.0 18-19 38.28324524249855 40.0 37.0 40.0 37.0 40.0 20-21 38.25414830794196 40.0 37.0 40.0 37.0 40.0 22-23 38.124485265392536 40.0 37.0 40.0 37.0 40.0 24-25 38.12217405533343 40.0 37.0 40.0 37.0 40.0 26-27 38.06370185414746 40.0 37.0 40.0 37.0 40.0 28-29 37.92242010186611 40.0 37.0 40.0 33.0 40.0 30-31 37.904682579712684 40.0 37.0 40.0 33.0 40.0 32-33 37.774531087658346 40.0 37.0 40.0 33.0 40.0 34-35 37.649125047157966 40.0 37.0 40.0 33.0 40.0 36-37 37.463643393791926 40.0 37.0 40.0 33.0 40.0 38-39 37.51333796375948 40.0 37.0 40.0 33.0 40.0 40-41 37.35506308669697 40.0 37.0 40.0 33.0 40.0 42-43 37.209841142398346 40.0 37.0 40.0 33.0 40.0 44-45 37.169144859630606 40.0 37.0 40.0 33.0 40.0 46-47 37.13981346277613 40.0 37.0 40.0 33.0 40.0 48-49 36.89818977133309 37.0 37.0 40.0 33.0 40.0 50-51 36.8071675042771 37.0 37.0 40.0 33.0 40.0 52-53 36.71151734359282 37.0 37.0 40.0 33.0 40.0 54-55 36.41201455071387 37.0 37.0 40.0 33.0 40.0 56-57 36.31437775094941 37.0 37.0 40.0 33.0 40.0 58-59 36.10636271856666 37.0 37.0 40.0 33.0 40.0 60-61 35.84520292589341 37.0 37.0 40.0 30.0 40.0 62-63 35.576033917463064 37.0 35.0 40.0 27.0 40.0 64-65 35.3405613076428 37.0 33.0 38.5 27.0 40.0 66-67 34.906823865876454 37.0 33.0 37.0 27.0 40.0 68-69 34.68176857117042 37.0 33.0 37.0 27.0 40.0 70-71 34.238506159822904 37.0 33.0 37.0 27.0 40.0 72-73 33.79272340131433 37.0 33.0 37.0 27.0 40.0 74-75 33.22156703489502 37.0 33.0 37.0 24.5 37.0 76-77 30.669807971293316 33.0 30.0 35.0 22.0 37.0 78-79 32.80779752979673 35.0 33.0 37.0 27.0 37.0 80-81 33.103650880246626 37.0 33.0 37.0 27.0 37.0 82-83 33.05358860477287 37.0 33.0 37.0 27.0 37.0 84-85 32.906623224969266 37.0 33.0 37.0 27.0 37.0 86-87 32.75868576611251 37.0 33.0 37.0 27.0 37.0 88-89 32.53521974999293 37.0 33.0 37.0 22.0 37.0 90-91 32.286312695586076 35.0 33.0 37.0 22.0 37.0 92-93 32.27448084573737 33.0 33.0 37.0 22.0 37.0 94-95 32.19854030472256 33.0 33.0 37.0 22.0 37.0 96-97 32.03174717546456 33.0 33.0 37.0 22.0 37.0 98-99 31.87583865203295 33.0 33.0 37.0 22.0 37.0 100-101 30.331704514763526 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.1659812770780391 2306 2 0.14734172819699864 2306 3 0.12002651601276426 2306 4 0.1537579095950008 2306 5 0.19391887249888384 2306 6 0.18173361745522243 2306 7 0.15490513550089702 2306 8 0.159885451025076 2306 9 0.16620719852900834 2306 10-11 0.17469475073507112 2306 12-13 0.19926059234348514 2306 14-15 0.3895905996895266 2306 16-17 0.3988647367363214 2306 18-19 0.40702462971032816 2306 20-21 0.36842508969329657 2306 22-23 0.42105817498293874 2306 24-25 0.43466653767882946 2306 26-27 0.3948219685581833 2306 28-29 0.5059697369436051 2306 30-31 0.4799773851035738 2306 32-33 0.4417742005784717 2306 34-35 0.48952108375933534 2306 36-37 0.5652486793041547 2306 38-39 0.5353333936013627 2306 40-41 0.5150317357295222 2306 42-43 0.37758342792321287 2306 44-45 0.38995769746809117 2306 46-47 0.44799526004035783 2306 48-49 0.573642454131658 2306 50-51 0.42721070296895647 2306 52-53 0.40498238590137703 2306 54-55 0.44653000936943954 2306 56-57 0.5204443971973447 2306 58-59 0.5010650727940416 2306 60-61 0.43392476221868037 2306 62-63 0.4042207381960381 2306 64-65 0.40977587450964137 2306 66-67 0.38676810550173 2306 68-69 0.4449478256681729 2306 70-71 0.41544090339711914 2306 72-73 0.3689513686681778 2306 74-75 0.3330608545301885 2306 76-77 0.048905633300460494 2306 78-79 0.5746097954595157 2306 80-81 0.4810283113471243 2306 82-83 0.4488848228550353 2306 84-85 0.5578233040694371 2306 86-87 0.4880066853173517 2306 88-89 0.6328793301276079 2306 90-91 0.6834637076831385 2306 92-93 0.5920010694669813 2306 94-95 0.4812503567733941 2306 96-97 0.5383548246561816 2306 98-99 0.5123169006958079 2306 100-101 0.4124694579166537 2307 1 0.1255180536891416 2307 2 0.13669303116361675 2307 3 0.10507133771321975 2307 4 0.07578789603660141 2307 5 0.14818524898361574 2307 6 0.16571601347457943 2307 7 0.10479763362509686 2307 8 0.13069026710911658 2307 9 0.19148707637177864 2307 10-11 0.22092944268734982 2307 12-13 0.18815790830685586 2307 14-15 0.3435086042792008 2307 16-17 0.2913487321203192 2307 18-19 0.30507322933384984 2307 20-21 0.2506337705794621 2307 22-23 0.31436080812071765 2307 24-25 0.32474051652070557 2307 26-27 0.35010877917498107 2307 28-29 0.37646951097027426 2307 30-31 0.36255108970597405 2307 32-33 0.3012057789254001 2307 34-35 0.4302657865495618 2307 36-37 0.34909621531728163 2307 38-39 0.3306357590814173 2307 40-41 0.34675180962061347 2307 42-43 0.33868657268217106 2307 44-45 0.3041882911855893 2307 46-47 0.36719665273813007 2307 48-49 0.40549131088211254 2307 50-51 0.32341917762703076 2307 52-53 0.1876589412780021 2307 54-55 0.4390842539383968 2307 56-57 0.47367267852756356 2307 58-59 0.5030307336234259 2307 60-61 0.33012869547098234 2307 62-63 0.2862459857794448 2307 64-65 0.26880353101262955 2307 66-67 0.171650160586573 2307 68-69 0.16877149187703822 2307 70-71 0.22467540228480232 2307 72-73 0.19201381190060118 2307 74-75 0.22359632356744896 2307 76-77 -0.01709378311792875 2307 78-79 0.3415763119160786 2307 80-81 0.4031668527441141 2307 82-83 0.3553222326302503 2307 84-85 0.28799311315489007 2307 86-87 0.24026823957509436 2307 88-89 0.4240132335865354 2307 90-91 0.4519482383826059 2307 92-93 0.2702776388846715 2307 94-95 0.37254246350301656 2307 96-97 0.2446884622125154 2307 98-99 0.21574079156165737 2307 100-101 0.2445309051112936 2201 1 0.15061382383736088 2201 2 0.14805460556124217 2201 3 0.11405523794560679 2201 4 0.1295914636399047 2201 5 0.14655681723269964 2201 6 0.16059600694178044 2201 7 0.15260616170461105 2201 8 0.07232967405727919 2201 9 0.11545211270373557 2201 10-11 0.15654479718960346 2201 12-13 0.14111543139219407 2201 14-15 0.2508469950701411 2201 16-17 0.2704354884589222 2201 18-19 0.21218183941154933 2201 20-21 0.27439121782372666 2201 22-23 0.2439682465775661 2201 24-25 0.20615930464524013 2201 26-27 0.24433648717573675 2201 28-29 0.29519392525050847 2201 30-31 0.25613319906684495 2201 32-33 0.2482274496630481 2201 34-35 0.25762704213104115 2201 36-37 0.26704314848230837 2201 38-39 0.17313416041643848 2201 40-41 0.23634028210207703 2201 42-43 0.2655515361517615 2201 44-45 0.17540357688810104 2201 46-47 0.30807334989729895 2201 48-49 0.3972524967184796 2201 50-51 0.34508455684527917 2201 52-53 0.2687878528219727 2201 54-55 0.4250979065556848 2201 56-57 0.37116376978791266 2201 58-59 0.33064933626899773 2201 60-61 0.3822247996345496 2201 62-63 0.3586292942378648 2201 64-65 0.33599607319209923 2201 66-67 0.31646526820189536 2201 68-69 0.29813075982885806 2201 70-71 0.4759550983043539 2201 72-73 0.2167322714415718 2201 74-75 0.2571076748121044 2201 76-77 0.11584898820046874 2201 78-79 0.4983269567303097 2201 80-81 0.46156558637896694 2201 82-83 0.4169810488973198 2201 84-85 0.35960627522559463 2201 86-87 0.400857921512376 2201 88-89 0.5080587791474329 2201 90-91 0.6007085125530907 2201 92-93 0.5078136243821163 2201 94-95 0.3264344512463566 2201 96-97 0.36962774187463054 2201 98-99 0.35018190508901625 2201 100-101 0.3844441328400343 1101 1 0.12079451223463167 1101 2 0.17783646088766147 1101 3 0.10361426316565314 1101 4 0.10882131653377769 1101 5 0.17502441791239676 1101 6 0.1769488432522266 1101 7 0.15314848478591614 1101 8 0.13326880787258233 1101 9 0.10837502870656834 1101 10-11 0.1608420930918868 1101 12-13 0.13471848842847578 1101 14-15 0.2775080100680114 1101 16-17 0.27148269722438556 1101 18-19 0.22308144590542156 1101 20-21 0.24829988182668927 1101 22-23 0.2613025732573888 1101 24-25 0.28862935954171576 1101 26-27 0.29425279014459704 1101 28-29 0.31899416050764984 1101 30-31 0.30817818526296037 1101 32-33 0.2731027782219897 1101 34-35 0.29787998451599407 1101 36-37 0.36631468432141645 1101 38-39 0.39337225093473904 1101 40-41 0.3728611407736153 1101 42-43 0.32757928075568543 1101 44-45 0.44699635557478246 1101 46-47 0.4540892990969212 1101 48-49 0.48717584086315924 1101 50-51 0.30824066422139396 1101 52-53 0.3585410012353236 1101 54-55 0.47439950369960826 1101 56-57 0.32862954383224263 1101 58-59 0.5071157088963432 1101 60-61 0.4698144516283591 1101 62-63 0.3308174993395241 1101 64-65 0.4540048469472495 1101 66-67 0.375428753354349 1101 68-69 0.4580315090034688 1101 70-71 0.5627954171010643 1101 72-73 0.38702122811172046 1101 74-75 0.31827736107010907 1101 76-77 0.08578938250253643 1101 78-79 0.3803983061791314 1101 80-81 0.4540265101753391 1101 82-83 0.3945861414103575 1101 84-85 0.4620344274232835 1101 86-87 0.3761889045398803 1101 88-89 0.4507594363276155 1101 90-91 0.4668791440912017 1101 92-93 0.38950750367541787 1101 94-95 0.28963481140053915 1101 96-97 0.2910776637582799 1101 98-99 0.3186383021131576 1101 100-101 0.3407767223717677 2304 1 0.14180554394049238 2304 2 0.15652152505046502 2304 3 0.09920839380288271 2304 4 0.10632590321869628 2304 5 0.12474253078552522 2304 6 0.104470952207258 2304 7 0.14563456269362973 2304 8 0.08619371234360784 2304 9 0.064944737448414 2304 10-11 0.07230589434718127 2304 12-13 0.1256057019460286 2304 14-15 0.22135678486147725 2304 16-17 0.2117990537375718 2304 18-19 0.1389924105288074 2304 20-21 0.17377959492116446 2304 22-23 0.22716729458968388 2304 24-25 0.20065708315907216 2304 26-27 0.15507894460385785 2304 28-29 0.1787868540218156 2304 30-31 0.18833428929803375 2304 32-33 0.24335526091385873 2304 34-35 0.32332978678612534 2304 36-37 0.2359163262359445 2304 38-39 0.17948790511130142 2304 40-41 0.23304126092259025 2304 42-43 0.2960145979985569 2304 44-45 0.3059280814205323 2304 46-47 0.2698608197534256 2304 48-49 0.3229871553994599 2304 50-51 0.33866135139666653 2304 52-53 0.3128101825035614 2304 54-55 0.4295185092496894 2304 56-57 0.3719327969570543 2304 58-59 0.25547088685252817 2304 60-61 0.30046218177870543 2304 62-63 0.3449285707630949 2304 64-65 0.3251087654803442 2304 66-67 0.3242093583041239 2304 68-69 0.26964711068974623 2304 70-71 0.32707204363261866 2304 72-73 0.16825052490725767 2304 74-75 0.33122474203396735 2304 76-77 0.2913704242448496 2304 78-79 0.48228715604657424 2304 80-81 0.5484618097157465 2304 82-83 0.45105588762713467 2304 84-85 0.4410556269051682 2304 86-87 0.4493154570543112 2304 88-89 0.4914855344598834 2304 90-91 0.6077071301564629 2304 92-93 0.31781102498204916 2304 94-95 0.39731987968922056 2304 96-97 0.42625792760757264 2304 98-99 0.32182428802093455 2304 100-101 0.3639808853999362 2202 1 0.12988739084893552 2202 2 0.13161249620409876 2202 3 0.061466624919560786 2202 4 0.06395339118638788 2202 5 0.1372594968774834 2202 6 0.06485168650090856 2202 7 0.09963952221181671 2202 8 0.13951085445442146 2202 9 0.11076323505561447 2202 10-11 0.10106755768552489 2202 12-13 0.10428812496199669 2202 14-15 0.14378320565186442 2202 16-17 0.1440927221134345 2202 18-19 0.12712657687828965 2202 20-21 0.1697817594701334 2202 22-23 0.1651887560741443 2202 24-25 0.13009107536027642 2202 26-27 0.1572375734345215 2202 28-29 0.15981726742068503 2202 30-31 0.18027726120678977 2202 32-33 0.16441656715999642 2202 34-35 0.26601908764450144 2202 36-37 0.28516309874829915 2202 38-39 0.2247773876665633 2202 40-41 0.18525979758614142 2202 42-43 0.28915569339883973 2202 44-45 0.2226730254886462 2202 46-47 0.2230735750441113 2202 48-49 0.2912002343907716 2202 50-51 0.18316119014334475 2202 52-53 0.18526467172054595 2202 54-55 0.29014565401511305 2202 56-57 0.2992123889987397 2202 58-59 0.39114790008803624 2202 60-61 0.37783026415360865 2202 62-63 0.30453812810380754 2202 64-65 0.2644544311886037 2202 66-67 0.22822266903325072 2202 68-69 0.2302108386543935 2202 70-71 0.3333800104225233 2202 72-73 0.20770352921392998 2202 74-75 0.2943694187041359 2202 76-77 0.09455150264289003 2202 78-79 0.4373899780925541 2202 80-81 0.4994389909439221 2202 82-83 0.41596605937552766 2202 84-85 0.3673034729361717 2202 86-87 0.3396993295194477 2202 88-89 0.44209143836138765 2202 90-91 0.5238700432445782 2202 92-93 0.42249589180511293 2202 94-95 0.2946985878026567 2202 96-97 0.26141293377712316 2202 98-99 0.18861529309680236 2202 100-101 0.25594958089568465 1102 1 0.1295420110643022 1102 2 0.12919433054605634 1102 3 0.08921898102082793 1102 4 0.0555591028855531 1102 5 0.123068559166299 1102 6 0.12538703784623095 1102 7 0.1091679596218782 1102 8 0.13243564052562107 1102 9 0.07145992846258764 1102 10-11 0.09353316868696027 1102 12-13 0.11867951988298842 1102 14-15 0.23843764835927317 1102 16-17 0.15316471046892133 1102 18-19 0.16033657387333022 1102 20-21 0.1645680969355965 1102 22-23 0.1430871441750199 1102 24-25 0.15034029654407277 1102 26-27 0.0809655386733894 1102 28-29 0.19346757926414426 1102 30-31 0.22657894613776364 1102 32-33 0.14235977602220373 1102 34-35 0.19962540287075115 1102 36-37 0.12442969922985014 1102 38-39 0.16982380599958447 1102 40-41 0.2467627575314708 1102 42-43 0.26030773277518193 1102 44-45 0.14718817515956317 1102 46-47 0.2793196999818264 1102 48-49 0.24459821232144918 1102 50-51 0.18853007705428837 1102 52-53 0.1057762243193281 1102 54-55 0.211114796791108 1102 56-57 0.25571044845630553 1102 58-59 0.1258742588798185 1102 60-61 0.09388367249673735 1102 62-63 0.17560990852138048 1102 64-65 0.18738066268181086 1102 66-67 0.16269496954915752 1102 68-69 0.06832037248043576 1102 70-71 0.13361644469322442 1102 72-73 0.10887406745612083 1102 74-75 -0.025948301535706264 1102 76-77 -0.24198689549986696 1102 78-79 0.14589990015387855 1102 80-81 0.1781996873837457 1102 82-83 0.10963438193792996 1102 84-85 0.1262206172894551 1102 86-87 0.11484615396533826 1102 88-89 0.36215510032536 1102 90-91 0.33437518653787635 1102 92-93 0.16018542910524047 1102 94-95 0.06270125549379912 1102 96-97 0.057027757974502435 1102 98-99 0.015966005727889865 1102 100-101 0.006715020601934185 2305 1 0.09707913344013264 2305 2 0.09933713079638551 2305 3 0.0327702127441043 2305 4 0.01205338518629162 2305 5 0.08361927885680132 2305 6 0.029420718223995834 2305 7 0.029728919967780598 2305 8 0.07461576227563427 2305 9 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-1.3290698092752464 1213 86-87 -1.472659651382962 1213 88-89 -1.5129938131883343 1213 90-91 -1.316348510713027 1213 92-93 -1.4892791043762443 1213 94-95 -1.4611847325294605 1213 96-97 -1.5629217336867711 1213 98-99 -1.5554287102115367 1213 100-101 -1.50663409749151 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 3.0 9 26.0 10 37.0 11 38.0 12 57.0 13 180.0 14 452.0 15 1180.0 16 2238.0 17 3678.0 18 5563.0 19 8055.0 20 10813.0 21 14511.0 22 19098.0 23 24643.0 24 31283.0 25 40954.0 26 53706.0 27 76246.0 28 117747.0 29 156553.0 30 129831.0 31 129139.0 32 171712.0 33 241029.0 34 369867.0 35 637619.0 36 1248530.0 37 2026534.0 38 598563.0 39 501.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.10410535704319 25.30039567162447 22.118752556528435 15.47674641480391 2 28.87469370809395 25.73717969141494 31.6054524003139 13.782674200177212 3 35.95061880890865 19.44970146495638 28.114905152239555 16.484774573895407 4 28.041018219989255 19.904516752706822 32.04751057098603 20.006954456317892 5 34.623072037764935 18.55369603262016 30.18804203067551 16.635189898939398 6 28.85778301274086 19.48831013463691 34.818942004811134 16.834964847811094 7 31.09602382986566 23.075027771936643 27.578256080865476 18.25069231733222 8 27.760417764432216 29.140689959638173 25.40414519539369 17.69474708053592 9 31.987960892015487 23.60514130887475 25.82451077031567 18.582387028794095 10-11 24.43712266655774 30.053519055594137 29.214117070381114 16.295241207467015 12-13 22.718849314593996 28.47833805280614 29.16009232749498 19.64272030510489 14-15 21.923286877120677 28.78826943459621 29.694069999168356 19.594373689114757 16-17 23.129571364861444 27.332234978988602 30.351554812426734 19.186638843723216 18-19 20.72169619339431 27.45419529843456 32.3704367060449 19.453671802126237 20-21 22.709152215374615 27.810185859162175 31.856947934828305 17.623713990634908 22-23 20.871466134412742 31.545390838847283 29.73810316243074 17.845039864309236 24-25 20.725527650457398 27.451875183709788 31.745108601792666 20.07748856404015 26-27 20.654943878548856 27.714432110257082 29.539692506372557 22.09093150482151 28-29 22.25306994476003 29.644187532585764 29.45823523904616 18.644507283608046 30-31 19.980942381584693 28.064932822058474 31.343761432079376 20.610363364277454 32-33 19.989226171482294 30.053843000450787 29.757399327852962 20.199531500213958 34-35 22.240864834200842 29.85791584747827 29.79171905305988 18.109500265261 36-37 20.25179126744763 29.672045248119115 31.82497283260029 18.251190651832964 38-39 20.829149136064704 27.528777419223072 31.13528042155141 20.506793023160817 40-41 22.53975339752221 27.76618148511649 30.717907964715423 18.97615715264587 42-43 20.3985909689074 29.816907327399573 31.110338264844124 18.674163438848908 44-45 20.14400249791248 27.872834229570543 31.425799393674552 20.557363878842423 46-47 22.25839339690059 27.726782194925814 29.37368448185528 20.64113992631832 48-49 20.409338922974456 30.011738769246254 30.739325138417474 18.839597169361813 50-51 22.65753330648172 27.959985653723702 30.27020182008168 19.112279219712892 52-53 20.57989329390842 27.807301256146253 28.433478954533896 23.179326495411427 54-55 22.602520062291912 28.02424348002579 30.111695813876644 19.261540643805652 56-57 20.519125084734906 28.241016139016885 30.2444532547682 20.995405521480013 58-59 20.50453929945109 28.233879560474186 30.557556015908855 20.70402512416587 60-61 22.211263317316625 28.502608101334587 30.42487447332565 18.861254108023136 62-63 20.177131346406576 28.320132227569662 30.394464482019494 21.108271944004272 64-65 22.06394342228318 28.099274100363086 30.348719760514076 19.48806271683966 66-67 20.37297579360559 31.27526657529012 28.62276746718379 19.728990163920496 68-69 20.195081627489962 31.689691983947103 28.57580736788362 19.539419020679315 70-71 19.9667393027168 32.30893240369213 28.30084759128387 19.4234807023072 72-73 19.95377387390413 32.15227591274169 28.156075848695806 19.737874364658374 74-75 20.196295246342753 31.428364092689364 28.410534292543527 19.96480636842435 76-77 20.110682290735422 31.157550247654935 28.54811906133991 20.183648400269735 78-79 19.941130582721925 31.064007151989497 28.68422693299945 20.310635332289124 80-81 19.825879064882063 31.171689984046935 28.48179368469076 20.52063726638024 82-83 19.543957362755936 30.860484719415755 28.638659356111475 20.956898561716837 84-85 19.72650096929498 30.608806139696128 28.85292304856671 20.811769842442178 86-87 20.011829206716694 30.50189062200332 29.130173326969295 20.35610684431069 88-89 20.29559282811098 30.597804503758034 28.689280245273245 20.41732242285774 90-91 20.306192512401555 30.333514378727628 28.604123667704595 20.756169441166225 92-93 19.89464901298118 30.934321257987325 28.70875284755867 20.462276881472825 94-95 19.602307393235577 31.07519656887268 28.734596080726888 20.58789995716485 96-97 19.82675151781812 31.18370527082426 28.60071764774721 20.388825563610418 98-99 20.32293879699291 30.652428068394634 28.197123920836926 20.82750921377553 100-101 20.45398153481624 30.597017730943925 27.68996249034798 21.25903824389185 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 146.0 1 91.0 2 48.0 3 86.0 4 104.0 5 84.0 6 65.0 7 63.0 8 67.0 9 64.0 10 71.5 11 76.0 12 78.5 13 102.5 14 114.0 15 126.0 16 182.0 17 272.0 18 380.0 19 586.5 20 895.5 21 1403.0 22 2261.5 23 3553.5 24 5543.0 25 8685.5 26 13680.0 27 20445.5 28 28555.0 29 37949.5 30 49027.0 31 61370.5 32 79337.0 33 108483.0 34 146506.0 35 195087.5 36 264522.0 37 337393.5 38 398877.0 39 431523.5 40 438355.0 41 427134.0 42 397508.5 43 369797.0 44 335169.0 45 302257.0 46 282031.5 47 253777.5 48 216787.0 49 186944.0 50 157002.5 51 128151.5 52 104270.0 53 81937.5 54 65045.0 55 51312.5 56 37433.5 57 25713.0 58 18087.0 59 13097.5 60 9428.5 61 6702.0 62 4588.5 63 3074.0 64 2148.5 65 1507.5 66 997.0 67 682.5 68 483.5 69 330.5 70 243.0 71 168.5 72 115.0 73 77.0 74 54.5 75 42.0 76 27.5 77 17.5 78 14.0 79 14.0 80 11.0 81 6.0 82 2.5 83 2.0 84 2.5 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18018468439985905 2 0.0 3 0.0 4 0.009313136571265836 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.002238420544321789 12-13 0.0 14-15 0.0 16-17 3.022684676638912E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.002238420544321789 40-41 0.006135232951772495 42-43 0.0024998419217608296 44-45 0.0012499209608804148 46-47 0.0067397698871002765 48-49 0.008087723864520332 50-51 0.005424493581860102 52-53 0.0065191955998860854 54-55 0.01852007070794706 56-57 0.021289503425191904 58-59 0.027065281982985717 60-61 0.02561929498902602 62-63 0.01036699149906697 64-65 0.015456538941083302 66-67 0.02261294914847705 68-69 0.026387220285253202 70-71 0.028658318501754872 72-73 0.021836854434204897 74-75 0.0318688997934281 76-77 0.029295533109262535 78-79 0.02043171453047005 80-81 0.009149748210366435 82-83 0.0016992389533537669 84-85 0.007760947142721531 86-87 0.026689488752917098 88-89 0.024459237626640276 90-91 0.021166962154517355 92-93 0.04324072971202638 94-95 0.04489095215711032 96-97 0.03373969652572623 98-99 0.041002309167704594 100-101 0.04076539604440046 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6120387.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.44091791289303 #Duplication Level Percentage of deduplicated Percentage of total 1 91.69849912375133 50.83848962655391 2 4.300498516323635 4.768471704560338 3 1.0246644309760742 1.7042500981801731 4 0.6460026277440691 1.4325991462508851 5 0.3694096492189362 1.0240205019288826 6 0.21564378889454397 0.7173293759116587 7 0.2524991472465819 0.9799149146901266 8 0.1465399867979026 0.6499449103215157 9 0.11434323479607687 0.5705364504801558 >10 0.9969111746489161 11.000776305609955 >50 0.12985816540898895 5.051233223304133 >100 0.08891338767078459 9.843037669666172 >500 0.012768183987127535 4.908179007218207 >1k 0.003330916149947257 2.5752605744127637 >5k 0.0 0.0 >10k+ 1.1766638489965975E-4 3.935956490911171 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 207976 3.3980857746413746 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 12389 0.20242184031826746 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 10532 0.17208062169924876 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 10442 0.17061012645115417 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.332806405869432E-4 0.0 0.0 2 8.986359849467036E-4 0.0 0.0 3 9.313136571265836E-4 0.0 0.0 4 9.476524932165237E-4 0.0 0.0 5 0.0011600573623857446 0.0 0.0 6 0.001274429215015325 0.0 0.0 7 0.0014051399037348455 0.0 1.6338836089940064E-5 8 0.0015685282646342462 0.0 1.6338836089940064E-5 9 0.001829949642073287 0.0 3.267767217988013E-5 10-11 0.002965498750324122 0.0 4.084709022485016E-5 12-13 0.004133725530754836 0.0 4.901650826982019E-5 14-15 0.0053754770735902815 0.0 4.901650826982019E-5 16-17 0.006886819411909737 0.0 6.535534435976026E-5 18-19 0.008569719529173564 0.0 6.535534435976026E-5 20-21 0.010628412876506012 0.0 6.535534435976026E-5 22-23 0.01276063098624319 0.0 6.535534435976026E-5 24-25 0.015505555449353121 0.0 7.352476240473029E-5 26-27 0.018283157584642933 0.0 8.169418044970032E-5 28-29 0.021346689351506694 0.0 1.0620243458461042E-4 30-31 0.031231685185920434 0.0 1.1437185262958044E-4 32-33 0.03538174955276521 0.0 1.1437185262958044E-4 34-35 0.039008971164731905 0.0 1.1437185262958044E-4 36-37 0.0429629694984974 0.0 1.2254127067455047E-4 38-39 0.04744798000518595 0.0 1.3071068871952052E-4 40-41 0.052316953159988085 0.0 1.3071068871952052E-4 42-43 0.057708769069668306 0.0 1.3071068871952052E-4 44-45 0.06348454762746211 0.0 1.3071068871952052E-4 46-47 0.06948906989051509 0.0 1.3071068871952052E-4 48-49 0.07558345575206274 0.0 1.3071068871952052E-4 50-51 0.08206997367976895 0.0 1.3071068871952052E-4 52-53 0.08898947076385856 0.0 1.3071068871952052E-4 54-55 0.09643998002087123 0.0 1.3071068871952052E-4 56-57 0.10422543541772766 0.0 1.3071068871952052E-4 58-59 0.11219878742961842 0.0 1.3888010676449056E-4 60-61 0.12065413510616241 0.0 1.4704952480946057E-4 62-63 0.12962415611953948 0.0 1.552189428544306E-4 64-65 0.1400156558727414 0.0 1.9606603307928077E-4 66-67 0.15106070906954086 0.0 2.287437052591609E-4 68-69 0.16307792301369178 0.0 2.287437052591609E-4 70-71 0.17622251664804855 0.0 2.287437052591609E-4 72-73 0.1904944899726112 0.0 2.287437052591609E-4 74-75 0.20639217748812289 0.0 2.369131233041309E-4 76-77 0.22507563655696936 0.0 2.4508254134910095E-4 78-79 0.24566257003029385 0.0 2.6142137743904103E-4 80-81 0.26918232458176256 0.0 2.6142137743904103E-4 82-83 0.29497317734973294 0.0 2.6142137743904103E-4 84-85 0.3250366357552227 0.0 2.6142137743904103E-4 86-87 0.35898873715011814 0.0 2.6142137743904103E-4 88-89 0.3969356839690039 0.0 2.6142137743904103E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 27390 0.0 81.71995 3 GATCGGA 27625 0.0 80.95671 1 CGGAAGA 27620 0.0 80.901886 4 ATCGGAA 27945 0.0 79.65509 2 GAAGAGC 29535 0.0 75.7355 6 GGAAGAG 33415 0.0 67.083565 5 AGAGCAC 33490 0.0 66.663895 8 GAGCACA 33850 0.0 66.15134 9 AAGAGCA 35450 0.0 62.96471 7 GCCGTCT 24440 0.0 45.325653 50-51 TATGCCG 24330 0.0 45.25576 48-49 TGCCGTC 24440 0.0 45.23819 50-51 CGTATGC 24395 0.0 45.210106 46-47 ATGCCGT 24420 0.0 45.088966 48-49 TCGTATG 24610 0.0 45.016685 44-45 TCGTATC 24230 0.0 44.809887 38-39 CTCGTAT 24510 0.0 44.764336 44-45 TCTCGTA 24510 0.0 44.71479 42-43 AATTCGT 24070 0.0 44.692284 34-35 CCGTCTT 24760 0.0 44.68376 52-53 >>END_MODULE