FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_35011-1.341000000358a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_35011-1.341000000358a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6120387
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2079763.3980857746413746TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC123890.20242184031826746No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG105320.17208062169924876No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA104420.17061012645115417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG273900.081.719953
GATCGGA276250.080.956711
CGGAAGA276200.080.9018864
ATCGGAA279450.079.655092
GAAGAGC295350.075.73556
GGAAGAG334150.067.0835655
AGAGCAC334900.066.6638958
GAGCACA338500.066.151349
AAGAGCA354500.062.964717
GCCGTCT244400.045.32565350-51
TATGCCG243300.045.2557648-49
TGCCGTC244400.045.2381950-51
CGTATGC243950.045.21010646-47
ATGCCGT244200.045.08896648-49
TCGTATG246100.045.01668544-45
TCGTATC242300.044.80988738-39
CTCGTAT245100.044.76433644-45
TCTCGTA245100.044.7147942-43
AATTCGT240700.044.69228434-35
CCGTCTT247600.044.6837652-53
Produced by FastQC (version 0.11.2)