FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_35010-1.341000000357eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_35010-1.341000000357eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6928491
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT718731.0373543099067315TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG146650.052.981153
CGGAAGA148100.052.4944954
GATCGGA153050.050.7071381
ATCGGAA153650.050.3510672
GAAGAGC174050.044.7743876
GCCGTCT93250.041.4907950-51
TATGCCG92750.041.45644448-49
CGTATGC93650.041.20841246-47
TGCCGTC93850.041.12430650-51
ATGCCGT94450.040.76056348-49
CTCGTAT95000.040.3719644-45
TCTCGTA95550.040.08798242-43
CCGTCTT97250.039.68969352-53
TCGTATG98500.039.371444-45
CATCTCG98500.038.69241340-41
GTATGCC100150.038.65244746-47
ATCTCGT99300.038.40668542-43
CGTCTTC101850.038.0603852-53
TCACGAG99650.037.8843530-31
CGAGATT101150.037.4876732-33
Produced by FastQC (version 0.11.2)