##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_35008-2.341000000358b3.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5863258 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65894269022444 33.0 33.0 33.0 33.0 33.0 2 32.67540606263616 33.0 33.0 33.0 33.0 33.0 3 32.735054810823605 33.0 33.0 33.0 33.0 33.0 4 36.68026223645625 37.0 37.0 37.0 37.0 37.0 5 36.63661773027897 37.0 37.0 37.0 37.0 37.0 6 36.54336496875969 37.0 37.0 37.0 37.0 37.0 7 36.483425597167994 37.0 37.0 37.0 37.0 37.0 8 36.43064197413793 37.0 37.0 37.0 37.0 37.0 9 36.45755892031359 37.0 37.0 37.0 37.0 37.0 10-11 36.40282288447822 37.0 37.0 37.0 37.0 37.0 12-13 36.38185885731107 37.0 37.0 37.0 37.0 37.0 14-15 38.246444212415696 40.0 37.0 40.0 37.0 40.0 16-17 38.24654168382152 40.0 37.0 40.0 37.0 40.0 18-19 38.21080216835077 40.0 37.0 40.0 37.0 40.0 20-21 38.178184466724815 40.0 37.0 40.0 37.0 40.0 22-23 38.0449242554225 40.0 37.0 40.0 35.0 40.0 24-25 38.03368315022126 40.0 37.0 40.0 35.0 40.0 26-27 37.96850181247355 40.0 37.0 40.0 33.0 40.0 28-29 37.8104119757309 40.0 37.0 40.0 33.0 40.0 30-31 37.77846105356441 40.0 37.0 40.0 33.0 40.0 32-33 37.64316289680583 40.0 37.0 40.0 33.0 40.0 34-35 37.51752984432887 40.0 37.0 40.0 33.0 40.0 36-37 37.33913312359783 40.0 37.0 40.0 33.0 40.0 38-39 37.37257656067668 40.0 37.0 40.0 33.0 40.0 40-41 37.204059244877165 40.0 37.0 40.0 33.0 40.0 42-43 37.06531172600626 40.0 37.0 40.0 33.0 40.0 44-45 37.02600388725858 40.0 37.0 40.0 33.0 40.0 46-47 36.997675609703684 40.0 37.0 40.0 33.0 40.0 48-49 36.75880568448463 37.0 37.0 40.0 33.0 40.0 50-51 36.681697274791595 37.0 37.0 40.0 33.0 40.0 52-53 36.59743200793825 37.0 37.0 40.0 33.0 40.0 54-55 36.28680001801047 37.0 37.0 40.0 33.0 40.0 56-57 36.18145815858692 37.0 37.0 40.0 33.0 40.0 58-59 35.979923192873315 37.0 37.0 40.0 30.0 40.0 60-61 35.76245996679661 37.0 37.0 40.0 27.0 40.0 62-63 35.51477045697119 37.0 33.0 40.0 27.0 40.0 64-65 35.316843468256046 37.0 33.0 40.0 27.0 40.0 66-67 34.91220964521773 37.0 33.0 40.0 27.0 40.0 68-69 34.70002206964115 37.0 33.0 37.0 27.0 40.0 70-71 34.26737651660561 37.0 33.0 37.0 27.0 40.0 72-73 33.836011906690786 37.0 33.0 37.0 27.0 40.0 74-75 33.26870666445174 37.0 33.0 37.0 22.0 40.0 76-77 30.760401810733896 33.0 30.0 35.0 22.0 37.0 78-79 32.817407318593176 37.0 33.0 37.0 22.0 37.0 80-81 33.068065826201064 37.0 33.0 37.0 22.0 37.0 82-83 32.96570976409362 37.0 33.0 37.0 22.0 37.0 84-85 32.78126188545686 37.0 33.0 37.0 22.0 37.0 86-87 32.60000071632529 37.0 33.0 37.0 22.0 37.0 88-89 32.33934512177359 37.0 33.0 37.0 22.0 37.0 90-91 32.055345253441004 33.0 33.0 37.0 22.0 37.0 92-93 32.02857046372512 33.0 33.0 37.0 22.0 37.0 94-95 31.93585392285313 33.0 33.0 37.0 22.0 37.0 96-97 31.759776816916464 33.0 33.0 37.0 18.5 37.0 98-99 31.58674162044379 33.0 33.0 37.0 15.0 37.0 100-101 29.998400462677917 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.1308662627683006 2306 2 0.1581737004788124 2306 3 0.10552942559405665 2306 4 0.12456631272104346 2306 5 0.18876237718541944 2306 6 0.17813773239493713 2306 7 0.19734256375166126 2306 8 0.1949156948098718 2306 9 0.2109778674756555 2306 10-11 0.2190355456613915 2306 12-13 0.1875328572816315 2306 14-15 0.38331595705940913 2306 16-17 0.3737853643192963 2306 18-19 0.3411274160077511 2306 20-21 0.3048820822249354 2306 22-23 0.3787258653884962 2306 24-25 0.32895384285952645 2306 26-27 0.35794798066470435 2306 28-29 0.42042491870790144 2306 30-31 0.32808210584318687 2306 32-33 0.35559483017856053 2306 34-35 0.31388353641500544 2306 36-37 0.35153979267998636 2306 38-39 0.3414202717401267 2306 40-41 0.3200013288706529 2306 42-43 0.21929697813775562 2306 44-45 0.3509435456103418 2306 46-47 0.34749275703737226 2306 48-49 0.4088390654970979 2306 50-51 0.3133051672231346 2306 52-53 0.21223269958225188 2306 54-55 0.28887020420925325 2306 56-57 0.2870391683868334 2306 58-59 0.311554356660686 2306 60-61 0.3569237193039996 2306 62-63 0.2885085994857306 2306 64-65 0.14271451372804478 2306 66-67 0.15359557937114232 2306 68-69 0.24617624356582013 2306 70-71 0.2731665606867395 2306 72-73 0.2247419770653991 2306 74-75 0.09851536664180571 2306 76-77 -0.22108603212807765 2306 78-79 0.2342889891451776 2306 80-81 0.2457435766415017 2306 82-83 0.2202558730863302 2306 84-85 0.1940871062849041 2306 86-87 0.20039634580930255 2306 88-89 0.34672771796276436 2306 90-91 0.3813798429287303 2306 92-93 0.23961040672276823 2306 94-95 0.21918844982898378 2306 96-97 0.23211538608230242 2306 98-99 0.16813167216463754 2306 100-101 0.17927975273436658 2307 1 0.16213836139890248 2307 2 0.1668060837366454 2307 3 0.09504138169459964 2307 4 0.12428224321412529 2307 5 0.221804135194823 2307 6 0.18648256031124788 2307 7 0.16969354796405867 2307 8 0.20548282033029608 2307 9 0.24207999264854152 2307 10-11 0.20989901706984426 2307 12-13 0.1987199150748964 2307 14-15 0.35827320095670245 2307 16-17 0.3550116216234258 2307 18-19 0.38838120786741825 2307 20-21 0.44919045830049953 2307 22-23 0.3891329806949031 2307 24-25 0.4283815780742728 2307 26-27 0.4305749285178706 2307 28-29 0.5167753903465169 2307 30-31 0.4877426958503577 2307 32-33 0.4920685588478477 2307 34-35 0.5865983273003081 2307 36-37 0.5569587775133797 2307 38-39 0.45362819521908904 2307 40-41 0.49649941051701063 2307 42-43 0.5113755802743682 2307 44-45 0.4561664548483648 2307 46-47 0.46682492003208864 2307 48-49 0.5241359066858067 2307 50-51 0.5004822592753939 2307 52-53 0.3496599340854871 2307 54-55 0.5516792156432118 2307 56-57 0.571628324798219 2307 58-59 0.5817563550532441 2307 60-61 0.5584150529988889 2307 62-63 0.5051775692311864 2307 64-65 0.44978790168041627 2307 66-67 0.40760280639075575 2307 68-69 0.450448648949866 2307 70-71 0.6065263152590106 2307 72-73 0.45260102219229026 2307 74-75 0.4448854786172234 2307 76-77 0.18350412529013127 2307 78-79 0.6162288436183374 2307 80-81 0.7397749173122179 2307 82-83 0.5599432499114982 2307 84-85 0.6014872105894042 2307 86-87 0.566668492305098 2307 88-89 0.6746775092497188 2307 90-91 0.7291336751616555 2307 92-93 0.5589686302332311 2307 94-95 0.5636147667772988 2307 96-97 0.5102371226647513 2307 98-99 0.44370315653915426 2307 100-101 0.46382377539166697 2201 1 0.13265776899083903 2201 2 0.1447455858079536 2201 3 0.10617389598887428 2201 4 0.13413312661682397 2201 5 0.1881184589499867 2201 6 0.17829409389348427 2201 7 0.199476509185466 2201 8 0.15727453777292766 2201 9 0.15580655350096606 2201 10-11 0.18752240406724496 2201 12-13 0.15689099809873852 2201 14-15 0.34393303834088584 2201 16-17 0.3562315900187656 2201 18-19 0.27801808302832853 2201 20-21 0.261498894253414 2201 22-23 0.2948126224781191 2201 24-25 0.28960879725517685 2201 26-27 0.36257362061743237 2201 28-29 0.38974450876067834 2201 30-31 0.329593215490803 2201 32-33 0.3298525823959295 2201 34-35 0.42356432604709937 2201 36-37 0.35825736375695527 2201 38-39 0.1897237670495855 2201 40-41 0.32976543935392755 2201 42-43 0.42363811545758523 2201 44-45 0.31400074470455763 2201 46-47 0.44441663192728953 2201 48-49 0.4947952228372259 2201 50-51 0.4400082275514663 2201 52-53 0.3348161740341382 2201 54-55 0.5064689142444578 2201 56-57 0.5358498170717141 2201 58-59 0.5082391766547119 2201 60-61 0.5093595654109606 2201 62-63 0.45510040672548513 2201 64-65 0.39627375822140465 2201 66-67 0.40441541056698327 2201 68-69 0.3042987490603082 2201 70-71 0.4050754047942746 2201 72-73 0.27140845532859714 2201 74-75 0.3297327114338202 2201 76-77 0.30716861615040614 2201 78-79 0.542635596055483 2201 80-81 0.42984172267099297 2201 82-83 0.48073086367953977 2201 84-85 0.4191809031055129 2201 86-87 0.4722804300860943 2201 88-89 0.6809535332813184 2201 90-91 0.7182168450767321 2201 92-93 0.5240722802176236 2201 94-95 0.45168063024816973 2201 96-97 0.4966314555266784 2201 98-99 0.3414460450139778 2201 100-101 0.6337688436712021 1101 1 0.08719401630805379 1101 2 0.1367333062700169 1101 3 0.09861157236336737 1101 4 0.10592194989221326 1101 5 0.14149324771066318 1101 6 0.09657398882235668 1101 7 0.07888016821702593 1101 8 0.12643176111099308 1101 9 0.13586474179054875 1101 10-11 0.13803642405807892 1101 12-13 0.1799867596672513 1101 14-15 0.31266646494600536 1101 16-17 0.2045112330444283 1101 18-19 0.19409290107557808 1101 20-21 0.20852292820798368 1101 22-23 0.2457933844181852 1101 24-25 0.23322199865972237 1101 26-27 0.2885665755290603 1101 28-29 0.2386188731838672 1101 30-31 0.2564109168330049 1101 32-33 0.2544258216319264 1101 34-35 0.30835794769559755 1101 36-37 0.2593582709104325 1101 38-39 0.24905792332682353 1101 40-41 0.2916944825908345 1101 42-43 0.2192532691796032 1101 44-45 0.2540213511982614 1101 46-47 0.3536162047463094 1101 48-49 0.3748559256525894 1101 50-51 0.25031830598258864 1101 52-53 0.24470397436169122 1101 54-55 0.3591801611099257 1101 56-57 0.2399318387396292 1101 58-59 0.36121039304033076 1101 60-61 0.18849805463069913 1101 62-63 0.15948579376169647 1101 64-65 0.18750466951213696 1101 66-67 0.19857619502332113 1101 68-69 0.23689652502083902 1101 70-71 0.27149279994113584 1101 72-73 0.13860161322283204 1101 74-75 0.2793810225579847 1101 76-77 0.015592273007225543 1101 78-79 0.3026075045665735 1101 80-81 0.3989287375652282 1101 82-83 0.3445653721931734 1101 84-85 0.36202142859092845 1101 86-87 0.35520944975495894 1101 88-89 0.43427434085145933 1101 90-91 0.3912330498434855 1101 92-93 0.31216823578316166 1101 94-95 0.20257410930322806 1101 96-97 0.30950880619708343 1101 98-99 0.24473567111255434 1101 100-101 0.29390067241685713 2304 1 0.1375021962988754 2304 2 0.14402572625689913 2304 3 0.10952080144310372 2304 4 0.11405765248964883 2304 5 0.1580334967338004 2304 6 0.056321415153483656 2304 7 0.1675190248708205 2304 8 0.05973693934461011 2304 9 0.10575772126659899 2304 10-11 0.034693255126860834 2304 12-13 0.11519894758635019 2304 14-15 0.22894596784654198 2304 16-17 0.2106796291941535 2304 18-19 0.2339010352832105 2304 20-21 0.19973467511357512 2304 22-23 0.2472420016726531 2304 24-25 0.22387188937184277 2304 26-27 0.23683606468217988 2304 28-29 0.2853345371988425 2304 30-31 0.2544600549568159 2304 32-33 0.24684580851364046 2304 34-35 0.3270414725396691 2304 36-37 0.2981939329570409 2304 38-39 0.35696034971089574 2304 40-41 0.38051186250964975 2304 42-43 0.30113944907449053 2304 44-45 0.38955246414069933 2304 46-47 0.289334436979793 2304 48-49 0.34735225521176716 2304 50-51 0.2839502577850439 2304 52-53 0.2007684395736291 2304 54-55 0.4284707335741089 2304 56-57 0.39228674498377103 2304 58-59 0.2491096800062067 2304 60-61 0.25257769120565854 2304 62-63 0.17094473247639286 2304 64-65 0.1888190517225965 2304 66-67 0.22486197053125068 2304 68-69 0.23317544239496613 2304 70-71 0.3082770639811585 2304 72-73 0.12884605804101312 2304 74-75 0.34775786237229056 2304 76-77 0.30152599764661403 2304 78-79 0.44164766482639806 2304 80-81 0.5159623860238938 2304 82-83 0.42163148622256585 2304 84-85 0.518359160224243 2304 86-87 0.47357118423368405 2304 88-89 0.5439594459939485 2304 90-91 0.6995280280824616 2304 92-93 0.5308184354538952 2304 94-95 0.42056926687523344 2304 96-97 0.5193804700202129 2304 98-99 0.37231327983515783 2304 100-101 0.30201438662889046 2202 1 0.15740399881609335 2202 2 0.14991664711787678 2202 3 0.09227608908538798 2202 4 0.028622787452697196 2202 5 0.14207517797463254 2202 6 0.11619666610464918 2202 7 0.15069803157696526 2202 8 0.18090783015042433 2202 9 0.1345604471139481 2202 10-11 0.13678876380920002 2202 12-13 0.1502453712555294 2202 14-15 0.23551546954836056 2202 16-17 0.2132998464753726 2202 18-19 0.1498450457414222 2202 20-21 0.21684394200070045 2202 22-23 0.1869921439338995 2202 24-25 0.20055701568030315 2202 26-27 0.20408123142512125 2202 28-29 0.20523927871842318 2202 30-31 0.22843565311629987 2202 32-33 0.23336901319407843 2202 34-35 0.360211323866757 2202 36-37 0.28428877804358166 2202 38-39 0.27548921274429716 2202 40-41 0.22593816576867454 2202 42-43 0.3573792894557286 2202 44-45 0.3246288965708146 2202 46-47 0.2862613426376228 2202 48-49 0.40451898346213255 2202 50-51 0.2630414795844018 2202 52-53 0.29833747857593806 2202 54-55 0.3839169853116502 2202 56-57 0.45559788304862536 2202 58-59 0.45327128801174155 2202 60-61 0.4471017497436236 2202 62-63 0.2749584235868596 2202 64-65 0.31352322276634226 2202 66-67 0.41375861297241556 2202 68-69 0.288089019290382 2202 70-71 0.38992724806518453 2202 72-73 0.11429480599701236 2202 74-75 0.23986724043878382 2202 76-77 -0.08356084697278021 2202 78-79 0.4226210856105226 2202 80-81 0.5355571208011654 2202 82-83 0.47807311231844096 2202 84-85 0.4137876193961816 2202 86-87 0.3981016447695467 2202 88-89 0.5251151816020609 2202 90-91 0.5928934622823547 2202 92-93 0.5035631523554684 2202 94-95 0.4507599945934899 2202 96-97 0.3781257325707692 2202 98-99 0.25892759358874073 2202 100-101 0.300928850638968 1102 1 0.07798370538635169 1102 2 0.12610028286866992 1102 3 0.07160260368357285 1102 4 0.04215934693059609 1102 5 0.10238865657819218 1102 6 0.09737066236064607 1102 7 0.06264433529646851 1102 8 0.047916807917374626 1102 9 0.06207713096719658 1102 10-11 0.04950941242507412 1102 12-13 0.12297746656623332 1102 14-15 0.195144051082778 1102 16-17 0.13059559729675385 1102 18-19 0.13657885477251597 1102 20-21 0.15837677017425733 1102 22-23 0.08303979417614471 1102 24-25 0.10381514959465932 1102 26-27 0.12870623103289347 1102 28-29 0.17697031729326085 1102 30-31 0.13767351019519936 1102 32-33 0.09058136499947977 1102 34-35 0.11485472969557975 1102 36-37 0.1790873878021415 1102 38-39 0.05814026025989705 1102 40-41 0.125610833789203 1102 42-43 0.1963447223492807 1102 44-45 0.12648491983024002 1102 46-47 0.1848979264231403 1102 48-49 0.11240837669024728 1102 50-51 0.03869782741738703 1102 52-53 0.11013541894438816 1102 54-55 0.1824569260378226 1102 56-57 0.21493665216333113 1102 58-59 0.13655944560104416 1102 60-61 0.02821553153938794 1102 62-63 0.07127683639746607 1102 64-65 0.24581282297780405 1102 66-67 0.16583439365327735 1102 68-69 0.06794950622078488 1102 70-71 0.0766222130592169 1102 72-73 0.10386991075659324 1102 74-75 0.07502803016002701 1102 76-77 -0.07624230711451219 1102 78-79 0.24857175930244324 1102 80-81 0.23153884272237235 1102 82-83 0.20781882580998 1102 84-85 0.13919760350318455 1102 86-87 0.08754477248003667 1102 88-89 0.21852626819959653 1102 90-91 0.2595881448671733 1102 92-93 0.1755400365412001 1102 94-95 0.07353677915517665 1102 96-97 -0.016250741092672882 1102 98-99 0.13297970750195987 1102 100-101 0.09839767963099533 2305 1 0.11166590742379157 2305 2 0.1327139834796398 2305 3 0.07701156807179643 2305 4 0.06379025146793538 2305 5 0.11936562627713698 2305 6 0.06748430217843548 2305 7 0.07762924284741501 2305 8 0.07264219373693237 2305 9 0.008834181347367576 2305 10-11 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-1.5488133343913155 1213 98-99 -1.5605098433578242 1213 100-101 -1.3754605761217178 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 4.0 8 8.0 9 23.0 10 41.0 11 48.0 12 82.0 13 226.0 14 608.0 15 1469.0 16 2875.0 17 4660.0 18 7015.0 19 9731.0 20 12817.0 21 16870.0 22 21574.0 23 27493.0 24 35001.0 25 44473.0 26 57575.0 27 80037.0 28 119690.0 29 154739.0 30 131518.0 31 135157.0 32 178207.0 33 246585.0 34 365142.0 35 595197.0 36 1057137.0 37 1752748.0 38 800308.0 39 4200.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.01713231026215 27.211850507192725 19.263669650059487 16.50734753248564 2 29.57193764968214 27.35327355541919 28.13006011333631 14.944728681562367 3 33.70233750587131 21.735765337291994 27.4913196724415 17.070577484395194 4 29.260758940077224 22.336611499525223 28.04110508945578 20.361524470941774 5 32.848511868316216 20.64751372018765 29.28893799317717 17.215036418318963 6 30.217193239662997 21.35488494621932 30.901761443893484 17.5261603702242 7 30.887758989967693 24.857391573081042 25.055302018093013 19.199547418858252 8 28.54691026729508 30.33951431098546 22.80486378051247 18.308711641206987 9 30.755119423364963 25.372924063720205 24.69343835799141 19.178518154923424 10-11 26.061175670373416 29.843071061940197 27.86738622526205 16.228367042424345 12-13 24.12758401557632 29.513582721415293 27.212779993648585 19.1460532693598 14-15 23.31678053396252 29.777233067349247 27.77448988258746 19.131496516100775 16-17 24.676204912388418 27.933850539057985 27.734694356147095 19.6552501924065 18-19 22.03699905726467 28.35001703404635 29.850914785850698 19.762069122838284 20-21 23.599353806364995 28.113345856518677 30.08268611069136 18.20461422642497 22-23 21.629655389546222 32.36288595862573 27.625494221813195 18.38196443001485 24-25 21.245628283797167 28.404770862888856 30.0897981975209 20.259802655793077 26-27 21.41067304218917 28.20136859063681 27.9137213474147 22.47423701975932 28-29 22.999422846478872 30.668358786190204 27.78711085202118 18.54510751530975 30-31 20.901783615866808 28.485869119182546 30.01052486518587 20.60182239976477 32-33 21.001625717305973 30.624031894895293 27.700588989943814 20.673753397854913 34-35 22.63889803245909 30.546455571288185 28.214612080860164 18.60003431539257 36-37 20.865813852980715 30.211658774012673 29.91817859626849 19.00434877673812 38-39 20.716572338567367 28.721522591712674 29.846611910403897 20.71529315931606 40-41 22.50127645907566 28.373764273763225 30.044260530861994 19.08069873629912 42-43 20.670695302552343 30.326126435127982 29.758023451549047 19.245154810770632 44-45 20.385776277798215 28.34448852169407 30.24412815478807 21.025607045719642 46-47 22.49206638489907 28.164024459353904 28.000776752748802 21.343132402998226 48-49 20.398588141421584 30.511873358124237 29.81527413200777 19.27426436844641 50-51 22.280674150116727 28.219318387753578 30.029520185060814 19.470487277068877 52-53 20.503336888189196 28.38611107439229 27.823699048546597 23.286852988871924 54-55 22.11393242121641 28.3728471317821 30.18811953053747 19.325100916464017 56-57 20.44075258955026 28.15714707683343 29.88867145168773 21.513428881928586 58-59 20.23848132423166 28.643255215739366 29.91737114896139 21.20089231106758 60-61 22.06019007013457 28.24669026947842 30.151195952185457 19.54192370820155 62-63 20.265404141885472 28.469705818739804 29.752258537274308 21.51263150210042 64-65 21.94161437093389 28.454695425865435 30.121199188104804 19.482491015095867 66-67 20.323174504013604 31.841161183883088 27.94261635709161 19.8930479550117 68-69 20.183988333712062 32.31408559599678 27.871260711100376 19.630665359190786 70-71 20.12882784201806 31.930403655624158 28.15103223238043 19.789736269977354 72-73 20.130427821713273 32.08377534432076 27.849059172309783 19.93673766165618 74-75 19.98505627228806 31.771151627567978 28.30970555789291 19.93408654225105 76-77 20.282483234712657 31.331484892304314 28.05303269666073 20.332999176322303 78-79 20.18608060570149 31.445721997816626 28.13330263861688 20.234894757865003 80-81 20.126877805594752 30.965425222304805 28.416176600396316 20.491520371704127 82-83 20.244849381872953 31.040947148681287 28.0459899110641 20.66821355838166 84-85 20.09148347442757 30.856650162912562 28.521216157958452 20.530650204701416 86-87 20.36356371876067 30.429149448081176 28.35001324714359 20.857273586014564 88-89 20.332810198677723 30.8826184949468 28.212064271608988 20.572507034766492 90-91 20.336612565883115 30.26779380352223 28.450245961632664 20.94534766896199 92-93 20.376414617622295 30.498255437526133 28.135705691453243 20.98962425339833 94-95 20.14792674253686 30.40256044839585 28.555468305288116 20.894044503779178 96-97 20.366043278194923 30.192769610488057 28.273365295396292 21.167821815920732 98-99 20.268413559004497 30.487549459162317 28.21219124894777 21.03184573288542 100-101 20.387608122146663 30.152331839877235 28.301753833377933 21.15830620459817 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 24.0 1 18.0 2 16.0 3 33.0 4 42.0 5 32.0 6 30.5 7 40.5 8 53.5 9 61.0 10 62.5 11 73.0 12 87.0 13 107.0 14 142.0 15 224.5 16 364.0 17 571.5 18 876.0 19 1462.0 20 2484.5 21 4009.0 22 6289.0 23 9662.5 24 14302.5 25 20203.5 26 27473.5 27 36700.5 28 47633.0 29 60235.5 30 74310.5 31 90341.5 32 109854.0 33 131533.5 34 155725.0 35 183675.0 36 215681.5 37 250730.0 38 283646.0 39 308833.0 40 325829.5 41 331783.5 42 324899.5 43 310119.5 44 291440.5 45 273133.0 46 257625.0 47 241008.5 48 219798.0 49 198020.0 50 177735.0 51 157535.5 52 136197.5 53 114719.5 54 95418.5 55 78609.0 56 64443.0 57 52401.5 58 42066.0 59 33410.5 60 25998.0 61 19772.5 62 14742.0 63 10776.5 64 7904.0 65 5722.5 66 4108.5 67 2940.0 68 2066.0 69 1440.0 70 1005.5 71 759.0 72 549.0 73 387.0 74 318.0 75 238.5 76 173.0 77 128.5 78 92.0 79 72.0 80 52.5 81 44.0 82 41.5 83 33.5 84 23.5 85 14.0 86 9.0 87 7.0 88 4.0 89 6.0 90 4.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18332810870679747 2 0.0 3 0.0 4 0.0090222876086981 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0021575035586017194 12-13 0.0 14-15 0.0 16-17 3.496349640421759E-4 18-19 8.527682049809168E-6 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0021063374663028646 40-41 0.006131403393812792 42-43 0.0025327215687933227 44-45 0.0011427093946744283 46-47 0.006626008952701723 48-49 0.008348600726763175 50-51 0.005466244193927676 52-53 0.006574842860402869 54-55 0.018888815740327306 56-57 0.02190761518595975 58-59 0.027714966661879795 60-61 0.0260435409801172 62-63 0.010471993557165658 64-65 0.01577621179214696 66-67 0.023510819411323877 68-69 0.026981586005596206 70-71 0.029241421748795637 72-73 0.022487497565346775 74-75 0.0330191848968611 76-77 0.02971044426153514 78-79 0.020952514796381126 80-81 0.009047870654847526 82-83 0.0017481748202108794 84-85 0.00768344152687806 86-87 0.027578523749082845 88-89 0.02521635582128571 90-91 0.021600618632166623 92-93 0.04467652625895023 94-95 0.04642470107916111 96-97 0.034767359717071976 98-99 0.04244227356190023 100-101 0.04208411091580824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5863258.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.04797284265925 #Duplication Level Percentage of deduplicated Percentage of total 1 96.97912256141453 85.3881514959235 2 2.039216906664242 3.5909782963652965 3 0.37891370176437666 1.0008774996798397 4 0.13020070117730687 0.4585563120541883 5 0.12069202203229681 0.5313343939112652 6 0.0777731446219083 0.41086606353347954 7 0.03139279359087003 0.19348502862808925 8 0.04638608893361704 0.3267360878963432 9 0.02292285007394846 0.18164794327086273 >10 0.15073537243915894 2.2972560453497093 >50 0.013782372911624936 0.8482067224987258 >100 0.008599052837412668 1.2211134985311853 >500 1.2931973623431077E-4 0.0754418803503457 >1k 1.138192459752322E-4 0.14680390310921562 >5k 0.0 0.0 >10k+ 1.929255651286512E-5 3.32854482889807 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 195950 3.341998595320213 TruSeq Adapter, Index 7 (97% over 35bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0013814844920690852 0.0 1.7055364099618336E-5 2 0.0015008720407664136 0.0 1.7055364099618336E-5 3 0.0016543703176629784 0.0 1.7055364099618336E-5 4 0.0018590346868583986 0.0 1.7055364099618336E-5 5 0.002285418789348857 0.0 1.7055364099618336E-5 6 0.002711802891839315 0.0 1.7055364099618336E-5 7 0.003342851363525194 0.0 1.7055364099618336E-5 8 0.003973899835211072 0.0 1.7055364099618336E-5 9 0.004587892942797332 0.0 1.7055364099618336E-5 10-11 0.006574842860402868 0.0 2.5583046149427503E-5 12-13 0.00828890695241451 0.0 3.411072819923667E-5 14-15 0.009994443362376345 0.0 3.411072819923667E-5 16-17 0.01188758877743398 0.0 5.1166092298855005E-5 18-19 0.014010981607836462 0.0 5.1166092298855005E-5 20-21 0.016731312181725584 0.0 9.380450254790085E-5 22-23 0.019818333083756506 0.0 1.2791523074713753E-4 24-25 0.023425542590825785 0.0 1.5349827689656502E-4 26-27 0.02713508428249277 0.0 1.5349827689656502E-4 28-29 0.031108984117703846 0.0 1.876090050958017E-4 30-31 0.04701311114059794 0.0 2.0466436919542002E-4 32-33 0.052513466062724855 0.0 2.0466436919542002E-4 34-35 0.05763860297466016 0.0 2.2171973329503835E-4 36-37 0.06330098385573345 0.0 2.5583046149427505E-4 38-39 0.06927036129059987 0.0 2.899411896935117E-4 40-41 0.07602428547404874 0.0 3.155242358429392E-4 42-43 0.0833580920368846 0.0 3.411072819923667E-4 44-45 0.09093920137916496 0.0 3.411072819923667E-4 46-47 0.09921958064952967 0.0 3.496349640421759E-4 48-49 0.10778137342753807 0.0 3.666903281417942E-4 50-51 0.11803164725140869 0.0 3.922733742912217E-4 52-53 0.12874241590596902 0.0 4.2638410249045837E-4 54-55 0.1399819008476175 0.0 4.775501947893134E-4 56-57 0.15164776989175643 0.0 4.775501947893134E-4 58-59 0.16524089507915224 0.0 5.201886050383592E-4 60-61 0.17904721231779328 0.0 5.287162870881684E-4 62-63 0.19425206941260303 0.0 5.372439691379775E-4 64-65 0.2102585286200948 0.0 5.542993332375959E-4 66-67 0.22754414013505803 0.0 5.62827015287405E-4 68-69 0.24740511162906356 0.0 5.713546973372143E-4 70-71 0.2679824084152531 0.0 5.969377434866417E-4 72-73 0.2915273385547762 1.7055364099618336E-5 5.969377434866417E-4 74-75 0.31718713384265196 1.7055364099618336E-5 6.139931075862601E-4 76-77 0.347349545252827 1.7055364099618336E-5 6.310484716858784E-4 78-79 0.381801380734056 1.7055364099618336E-5 6.310484716858784E-4 80-81 0.418760354737929 1.7055364099618336E-5 6.566315178353059E-4 82-83 0.46070802274094025 1.7055364099618336E-5 6.651591998851151E-4 84-85 0.508488625266021 1.7055364099618336E-5 6.651591998851151E-4 86-87 0.5611470619235926 1.7055364099618336E-5 6.907422460345426E-4 88-89 0.6195616839647855 1.7055364099618336E-5 7.077976101341609E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 27550 0.0 76.656555 3 CGGAAGA 27615 0.0 76.28695 4 GATCGGA 27935 0.0 75.62102 1 ATCGGAA 28065 0.0 75.04683 2 GAAGAGC 30305 0.0 69.49499 6 AGAGCAC 31455 0.0 66.81837 8 GAGCACA 31465 0.0 66.812225 9 AAGAGCA 34550 0.0 60.86025 7 GGAAGAG 37305 0.0 56.69664 5 TATGCCG 23195 0.0 44.78631 48-49 GCCGTCT 23370 0.0 44.768833 50-51 ATGCCGT 23325 0.0 44.63851 48-49 CGTATGC 23360 0.0 44.62959 46-47 TGCCGTC 23345 0.0 44.603127 50-51 TCTCGTA 23335 0.0 44.552425 42-43 CTCGTAT 23335 0.0 44.3506 44-45 CGTCTTC 23595 0.0 44.334694 52-53 TATCTCG 23345 0.0 44.23684 40-41 TCGTATC 23225 0.0 44.16928 38-39 ATCTCGT 23450 0.0 44.111153 42-43 >>END_MODULE