FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_35008-2.341000000358b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_35008-2.341000000358b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5863258
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT1959503.341998595320213TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG275500.076.6565553
CGGAAGA276150.076.286954
GATCGGA279350.075.621021
ATCGGAA280650.075.046832
GAAGAGC303050.069.494996
AGAGCAC314550.066.818378
GAGCACA314650.066.8122259
AAGAGCA345500.060.860257
GGAAGAG373050.056.696645
TATGCCG231950.044.7863148-49
GCCGTCT233700.044.76883350-51
ATGCCGT233250.044.6385148-49
CGTATGC233600.044.6295946-47
TGCCGTC233450.044.60312750-51
TCTCGTA233350.044.55242542-43
CTCGTAT233350.044.350644-45
CGTCTTC235950.044.33469452-53
TATCTCG233450.044.2368440-41
TCGTATC232250.044.1692838-39
ATCTCGT234500.044.11115342-43
Produced by FastQC (version 0.11.2)