##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_35008-1.341000000357b4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2422980 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.648328091853834 33.0 33.0 33.0 33.0 33.0 2 32.669610149485344 33.0 33.0 33.0 33.0 33.0 3 32.726460391749 33.0 33.0 33.0 33.0 33.0 4 36.67164524676225 37.0 37.0 37.0 37.0 37.0 5 36.6317493334654 37.0 37.0 37.0 37.0 37.0 6 36.529370857373976 37.0 37.0 37.0 37.0 37.0 7 36.486045695796086 37.0 37.0 37.0 37.0 37.0 8 36.42436586352343 37.0 37.0 37.0 37.0 37.0 9 36.4343539773337 37.0 37.0 37.0 37.0 37.0 10-11 36.39708850258772 37.0 37.0 37.0 37.0 37.0 12-13 36.374226984952415 37.0 37.0 37.0 37.0 37.0 14-15 38.207041535629685 40.0 37.0 40.0 37.0 40.0 16-17 38.194684025456255 40.0 37.0 40.0 37.0 40.0 18-19 38.207627178102996 40.0 37.0 40.0 37.0 40.0 20-21 38.16581234677959 40.0 37.0 40.0 37.0 40.0 22-23 38.07588465443379 40.0 37.0 40.0 37.0 40.0 24-25 38.03223901971952 40.0 37.0 40.0 35.0 40.0 26-27 37.93386614829673 40.0 37.0 40.0 33.0 40.0 28-29 37.82788962352144 40.0 37.0 40.0 33.0 40.0 30-31 37.79453214636522 40.0 37.0 40.0 33.0 40.0 32-33 37.68076232573112 40.0 37.0 40.0 33.0 40.0 34-35 37.603292845999555 40.0 37.0 40.0 33.0 40.0 36-37 37.53672935806321 40.0 37.0 40.0 33.0 40.0 38-39 37.4334614813164 40.0 37.0 40.0 33.0 40.0 40-41 37.345858818479726 40.0 37.0 40.0 33.0 40.0 42-43 37.211791058943945 40.0 37.0 40.0 33.0 40.0 44-45 37.1050089146423 40.0 37.0 40.0 33.0 40.0 46-47 37.05520350972769 38.5 37.0 40.0 33.0 40.0 48-49 36.837396924448406 37.0 37.0 40.0 33.0 40.0 50-51 36.67253464741764 37.0 37.0 40.0 33.0 40.0 52-53 36.55155799882789 37.0 37.0 40.0 33.0 40.0 54-55 36.308895244698675 37.0 37.0 40.0 33.0 40.0 56-57 36.18762391765512 37.0 37.0 40.0 33.0 40.0 58-59 35.94502141990442 37.0 37.0 40.0 33.0 40.0 60-61 35.68275202436669 37.0 35.0 40.0 27.0 40.0 62-63 35.39421022872661 37.0 33.0 40.0 27.0 40.0 64-65 35.14072113678198 37.0 33.0 37.0 27.0 40.0 66-67 34.793009228305635 37.0 33.0 37.0 27.0 40.0 68-69 34.519919272961396 37.0 33.0 37.0 27.0 40.0 70-71 34.09050631866545 37.0 33.0 37.0 27.0 40.0 72-73 33.78217422347687 37.0 33.0 37.0 27.0 37.0 74-75 33.36296399475027 37.0 33.0 37.0 27.0 37.0 76-77 30.78600504337634 33.0 30.0 35.0 24.5 37.0 78-79 33.049096360679826 35.0 33.0 37.0 27.0 37.0 80-81 33.42924724925505 37.0 33.0 37.0 27.0 37.0 82-83 33.42777633327555 37.0 33.0 37.0 27.0 37.0 84-85 33.29943231062576 37.0 33.0 37.0 27.0 37.0 86-87 33.164264253109806 37.0 33.0 37.0 27.0 37.0 88-89 32.96768586616481 37.0 33.0 37.0 27.0 37.0 90-91 32.710722952727636 33.0 33.0 37.0 27.0 37.0 92-93 32.68445302891481 33.0 33.0 37.0 27.0 37.0 94-95 32.621839016417795 33.0 33.0 37.0 27.0 37.0 96-97 32.45730546682185 33.0 33.0 37.0 27.0 37.0 98-99 32.296248834080345 33.0 33.0 37.0 22.0 37.0 100-101 30.743949805611273 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.20263115329152015 2306 2 0.1739205849987755 2306 3 0.1042803016614684 2306 4 0.1427878665477067 2306 5 0.1803989209449668 2306 6 0.13023962485644347 2306 7 0.2278378520814357 2306 8 0.1941784091454153 2306 9 0.20851280936054195 2306 10-11 0.13686990315098058 2306 12-13 0.2096862086895186 2306 14-15 0.4126982584927674 2306 16-17 0.3951663221985058 2306 18-19 0.42828011861244875 2306 20-21 0.4365821835792545 2306 22-23 0.3900033743557927 2306 24-25 0.4549740933723001 2306 26-27 0.4631310002016207 2306 28-29 0.48218135028516684 2306 30-31 0.3811497299689037 2306 32-33 0.47511056713902633 2306 34-35 0.4245462239278055 2306 36-37 0.49679595427939205 2306 38-39 0.49261064450374903 2306 40-41 0.39998983759225126 2306 42-43 0.32468565717639564 2306 44-45 0.3856975999630592 2306 46-47 0.47418366004022516 2306 48-49 0.5476428784718834 2306 50-51 0.4900106191825415 2306 52-53 0.1407022578692434 2306 54-55 0.33597348635139923 2306 56-57 0.44336991349148747 2306 58-59 0.5414651923037752 2306 60-61 0.3951219324570161 2306 62-63 0.45464978777282283 2306 64-65 0.3863496200323553 2306 66-67 0.34388241767936023 2306 68-69 0.35340778143007157 2306 70-71 0.32576508385482583 2306 72-73 0.4112368800445907 2306 74-75 0.35852352176768676 2306 76-77 -0.07885984611424135 2306 78-79 0.3490773431337857 2306 80-81 0.35958809916961343 2306 82-83 0.4408898868957891 2306 84-85 0.39386982148123906 2306 86-87 0.2494731079128627 2306 88-89 0.5272679784351837 2306 90-91 0.5130263982511636 2306 92-93 0.38771668411560256 2306 94-95 0.37502523903916085 2306 96-97 0.4891787122765763 2306 98-99 0.40868394952639164 2306 100-101 0.43646783869027317 2307 1 0.18319775516779657 2307 2 0.17738133392278854 2307 3 0.15683439833136958 2307 4 0.1295485619204797 2307 5 0.2020628769932884 2307 6 0.1471873528954788 2307 7 0.16963831420880382 2307 8 0.2649981893869935 2307 9 0.20938351286517332 2307 10-11 0.1965068951579667 2307 12-13 0.19734425922602838 2307 14-15 0.32632283454543654 2307 16-17 0.33295924252616516 2307 18-19 0.3802569279501995 2307 20-21 0.3041145126615348 2307 22-23 0.3526627884655511 2307 24-25 0.3008262274884075 2307 26-27 0.34075513495240983 2307 28-29 0.5088155308520186 2307 30-31 0.3832195102104734 2307 32-33 0.3489133498201511 2307 34-35 0.43891754228001645 2307 36-37 0.3176161064045786 2307 38-39 0.2600609112682619 2307 40-41 0.2823283409342494 2307 42-43 0.2593397558296857 2307 44-45 0.24139944351410492 2307 46-47 0.41948119172470655 2307 48-49 0.45637445783680164 2307 50-51 0.17164675833643628 2307 52-53 0.12423128462503286 2307 54-55 0.4385058898104077 2307 56-57 0.32463342526283867 2307 58-59 0.31567493962126036 2307 60-61 0.22253297789622906 2307 62-63 0.21073665482690984 2307 64-65 0.3132753573536107 2307 66-67 0.2597009831011192 2307 68-69 0.36969665566398646 2307 70-71 0.2593212581145323 2307 72-73 0.21102403865543096 2307 74-75 0.3084741852080555 2307 76-77 -0.05763074597341955 2307 78-79 0.296846909170398 2307 80-81 0.29979045344082067 2307 82-83 0.2635491274598607 2307 84-85 0.18164067671438744 2307 86-87 0.23625462876809422 2307 88-89 0.4791324789211089 2307 90-91 0.42869137573980964 2307 92-93 0.1606155416891326 2307 94-95 0.20695665378360673 2307 96-97 0.17501763212821686 2307 98-99 -0.04067145220707857 2307 100-101 0.1693594569294241 2201 1 0.1385652757074709 2201 2 0.14264550088383032 2201 3 0.12241121841410063 2201 4 0.051693661456702955 2201 5 0.14127237974899742 2201 6 0.13308805027574522 2201 7 0.2263187404616005 2201 8 0.20607102878062022 2201 9 0.21455499016423119 2201 10-11 0.12274758440972278 2201 12-13 0.103796522293365 2201 14-15 0.30512153436426104 2201 16-17 0.3560964044239725 2201 18-19 0.2331182618988663 2201 20-21 0.3816218625025769 2201 22-23 0.3819762145550172 2201 24-25 0.3400506625762034 2201 26-27 0.3533223897962756 2201 28-29 0.4184861981492105 2201 30-31 0.4167760688515756 2201 32-33 0.4726515796289874 2201 34-35 0.4387544498771305 2201 36-37 0.37084298574183805 2201 38-39 0.26868178101844364 2201 40-41 0.2972991314884652 2201 42-43 0.3611279322714509 2201 44-45 0.4382084112697626 2201 46-47 0.44872280492935346 2201 48-49 0.5347131753560106 2201 50-51 0.35135298310102314 2201 52-53 0.48372682344244566 2201 54-55 0.6390590982914048 2201 56-57 0.4542598368311488 2201 58-59 0.410855737164475 2201 60-61 0.4194605259360884 2201 62-63 0.4033614834255772 2201 64-65 0.36927243356025485 2201 66-67 0.29156346985431725 2201 68-69 0.29032941145555213 2201 70-71 0.35020064190537425 2201 72-73 0.4205074464367655 2201 74-75 0.46044568035944167 2201 76-77 0.22156920388813006 2201 78-79 0.49424533607651 2201 80-81 0.4934338380141412 2201 82-83 0.39976986103857115 2201 84-85 0.4700266221850242 2201 86-87 0.5757842864212321 2201 88-89 0.644300570925175 2201 90-91 0.6478094612866414 2201 92-93 0.5246000386523448 2201 94-95 0.44432708197356874 2201 96-97 0.4875413719248556 2201 98-99 0.3630009993732273 2201 100-101 0.3533910726409921 1101 1 0.17928305457706273 1101 2 0.18894515642601561 1101 3 0.13496324519961433 1101 4 0.09121326797421858 1101 5 0.11516917984788932 1101 6 0.1071767364496381 1101 7 0.14318142098512254 1101 8 0.20049963890515698 1101 9 0.07085619418241862 1101 10-11 0.14791935591535577 1101 12-13 0.08081169423444123 1101 14-15 0.2539639258359472 1101 16-17 0.2195788332375841 1101 18-19 0.21328304295015243 1101 20-21 0.24531570068371167 1101 22-23 0.2890324010290115 1101 24-25 0.2509919842607218 1101 26-27 0.33860885562516074 1101 28-29 0.36148298115685407 1101 30-31 0.38622211606536183 1101 32-33 0.2623879504742632 1101 34-35 0.3430549515030279 1101 36-37 0.38726832220068985 1101 38-39 0.3712467846575862 1101 40-41 0.4240160098322505 1101 42-43 0.4393886679626675 1101 44-45 0.45445926527435887 1101 46-47 0.47480848964803357 1101 48-49 0.390130476250782 1101 50-51 0.2926901746832087 1101 52-53 0.3557462860454095 1101 54-55 0.5517037435766028 1101 56-57 0.24816314766918168 1101 58-59 0.46627254718788436 1101 60-61 0.5515813400863863 1101 62-63 0.2740076218521992 1101 64-65 0.31447289426533587 1101 66-67 0.27584030255598435 1101 68-69 0.3927480461816586 1101 70-71 0.5331483957896168 1101 72-73 0.4272892804481714 1101 74-75 0.36027579424605705 1101 76-77 0.06837717903040286 1101 78-79 0.5389093859526142 1101 80-81 0.6001498283270124 1101 82-83 0.5047680247334583 1101 84-85 0.5386512184991119 1101 86-87 0.4982900909750043 1101 88-89 0.520643829231048 1101 90-91 0.6679925295271332 1101 92-93 0.6029812609487877 1101 94-95 0.5601747297849755 1101 96-97 0.5434042055109884 1101 98-99 0.5513073950253613 1101 100-101 0.7153047212297601 2304 1 0.15558461052709305 2304 2 0.168137345840222 2304 3 0.09389724858319681 2304 4 0.08084582374923599 2304 5 0.16468080988681066 2304 6 -0.006161763937889475 2304 7 0.1222940210967991 2304 8 0.12009235835911625 2304 9 0.08682197494930932 2304 10-11 0.12106442512887838 2304 12-13 0.05185098051424575 2304 14-15 0.2622143362833995 2304 16-17 0.21339878640074517 2304 18-19 0.23822803882485033 2304 20-21 0.18844185181171014 2304 22-23 0.20559781010135936 2304 24-25 0.21459467112889996 2304 26-27 0.20284325653856428 2304 28-29 0.1898855280145284 2304 30-31 0.25053214765106446 2304 32-33 0.24800978877880198 2304 34-35 0.35766000521538643 2304 36-37 0.2459098036451266 2304 38-39 0.4407163698764762 2304 40-41 0.34325974477904 2304 42-43 0.3393638744364793 2304 44-45 0.34062135243089386 2304 46-47 0.22158137261425992 2304 48-49 0.280396412862693 2304 50-51 0.3550661028778279 2304 52-53 0.3268080740128383 2304 54-55 0.5088244859656186 2304 56-57 0.5285668862829098 2304 58-59 0.4858215133603494 2304 60-61 0.44039050077343234 2304 62-63 0.5437680622185539 2304 64-65 0.45507281425933144 2304 66-67 0.39510612249248567 2304 68-69 0.4865136202663862 2304 70-71 0.5444275750613983 2304 72-73 0.38098351584915946 2304 74-75 0.24320763913442534 2304 76-77 0.3410445040797825 2304 78-79 0.38076148319323977 2304 80-81 0.44402770250090384 2304 82-83 0.32049253740523653 2304 84-85 0.4025418436293293 2304 86-87 0.28244619434941853 2304 88-89 0.378143350028445 2304 90-91 0.43561926819919705 2304 92-93 0.23147902328096137 2304 94-95 0.19255820021191994 2304 96-97 0.3234685436696836 2304 98-99 0.2929403091605849 2304 100-101 0.4568186681091113 2202 1 0.09786760498400326 2202 2 0.1307452106219955 2202 3 0.06624647528025207 2202 4 -0.029232634155306414 2202 5 0.07457825674077867 2202 6 0.04016994326338619 2202 7 0.10756624984728802 2202 8 0.1072358259373658 2202 9 0.12769189625401367 2202 10-11 0.1836396485736742 2202 12-13 0.09855153482349976 2202 14-15 0.13325405188833628 2202 16-17 0.20948716727173178 2202 18-19 0.0728453636595674 2202 20-21 0.03799971006522895 2202 22-23 0.06350235496243073 2202 24-25 0.10814118206671708 2202 26-27 0.15855905138479187 2202 28-29 0.11438433512203972 2202 30-31 0.08265686129752225 2202 32-33 0.15793453566006832 2202 34-35 0.09463878241155754 2202 36-37 0.20802211358352451 2202 38-39 0.09470009668628876 2202 40-41 0.11736952649751942 2202 42-43 0.1555091699296085 2202 44-45 0.20442656080131627 2202 46-47 0.17101430356893133 2202 48-49 0.2772883641024677 2202 50-51 0.37173454998105626 2202 52-53 0.3836268493098842 2202 54-55 0.22672173103678261 2202 56-57 0.3120560078155208 2202 58-59 0.25054216286361 2202 60-61 0.24816091941342222 2202 62-63 0.15475543298853012 2202 64-65 0.13322003262394588 2202 66-67 0.14569752417083492 2202 68-69 0.20468564604710338 2202 70-71 0.21792399638390236 2202 72-73 -0.05925049286310724 2202 74-75 0.12839173627504152 2202 76-77 0.024039257726713004 2202 78-79 0.4345891250643561 2202 80-81 0.337254921815088 2202 82-83 0.39224828524794475 2202 84-85 0.2742892827680734 2202 86-87 0.20726933765082833 2202 88-89 0.19623881703694934 2202 90-91 0.3452834778634113 2202 92-93 0.09918754207644298 2202 94-95 0.1662353358455846 2202 96-97 0.20678407263738308 2202 98-99 0.014521870959541161 2202 100-101 0.11399560789474705 1102 1 0.1314225894982286 1102 2 0.10509192443970505 1102 3 0.06854382727677688 1102 4 0.060075706373375226 1102 5 0.10036283656100409 1102 6 -0.037815610996439375 1102 7 0.07328313031045042 1102 8 0.1047899802629999 1102 9 0.05838949547243999 1102 10-11 0.0664764167549663 1102 12-13 0.07551609471460807 1102 14-15 0.13294387783540174 1102 16-17 0.1359612966917112 1102 18-19 0.15427665924683964 1102 20-21 0.13489470152827465 1102 22-23 0.10286923296025208 1102 24-25 0.04570045053755223 1102 26-27 0.21075142580443185 1102 28-29 0.11828272416629915 1102 30-31 0.07339750648248611 1102 32-33 0.07317503235135803 1102 34-35 0.12684413712798204 1102 36-37 0.10968088543187804 1102 38-39 0.029332205032780223 1102 40-41 -0.0055023985653477325 1102 42-43 0.06147769844326234 1102 44-45 0.08228449076505484 1102 46-47 0.1531770021863892 1102 48-49 0.15567691529724215 1102 50-51 0.02800379252569485 1102 52-53 0.01985431869093901 1102 54-55 0.20776069371383699 1102 56-57 0.15510994370064424 1102 58-59 0.169757015442066 1102 60-61 0.08806180131679753 1102 62-63 -0.08904366595870528 1102 64-65 -0.030047619490339628 1102 66-67 0.15923768225066937 1102 68-69 -0.006049242857834258 1102 70-71 -0.02843131516041808 1102 72-73 -0.04661333687824509 1102 74-75 0.23563054506907832 1102 76-77 -0.18133982541942828 1102 78-79 0.2697771960340134 1102 80-81 0.3244348720725725 1102 82-83 0.3188683283397822 1102 84-85 0.22781764162053975 1102 86-87 0.14506998001498062 1102 88-89 0.26505617669322845 1102 90-91 0.3630261790264697 1102 92-93 0.26851534158360124 1102 94-95 0.22659543588100917 1102 96-97 0.12733794832237066 1102 98-99 0.01761099812631528 1102 100-101 0.07882840630683674 2305 1 0.11221396715644971 2305 2 0.14803770074057354 2305 3 0.07094930063524885 2305 4 0.020467263370676392 2305 5 0.13538560559160118 2305 6 0.027305794529667082 2305 7 0.019914368717145692 2305 8 0.13678860005535398 2305 9 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-1.612422280174755 1213 100-101 -1.5669185047041658 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 12.0 9 33.0 10 31.0 11 16.0 12 27.0 13 77.0 14 208.0 15 477.0 16 934.0 17 1416.0 18 2232.0 19 2913.0 20 4268.0 21 5431.0 22 6939.0 23 8773.0 24 11345.0 25 14728.0 26 18897.0 27 25095.0 28 34985.0 29 45741.0 30 47490.0 31 54752.0 32 73928.0 33 104116.0 34 160050.0 35 279496.0 36 551893.0 37 801561.0 38 165058.0 39 57.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.609904445298966 25.4896196418456 24.759459336533652 15.141016576321784 2 28.772255652130845 23.324625048494003 33.9744446920734 13.928674607301753 3 36.84908666187917 19.406227042732503 26.848343775020844 16.896342520367483 4 27.897617846709093 19.273384222826305 34.76736919702522 18.061628733439385 5 32.24001023533005 18.231846734186828 32.67187512897342 16.85626790150971 6 25.6768111994321 19.41423371220563 38.12664570074866 16.7823093876136 7 31.475744744075477 20.794187323048476 29.69859429297807 18.03147363989798 8 27.545584363057063 28.092349090789025 26.672774847501834 17.689291698652074 9 30.32365104127975 24.563966685651554 25.971407110252663 19.140975162816037 10-11 24.23238624392235 28.76762675694912 29.06821515988286 17.93177183924567 12-13 23.681602819668342 26.883073735647837 29.035877308108198 20.399446136575623 14-15 22.97656191961964 27.404827939149314 29.826845454770574 19.791764686460475 16-17 22.630486008806134 27.646684101189017 30.62137983107925 19.101450058925604 18-19 20.91554614565535 27.943916169345183 31.481832289164586 19.65870539583488 20-21 21.799705321546195 28.100644660707065 31.01243097342941 19.087219044317326 22-23 21.124730703513855 29.458600566244876 30.32957350040033 19.08709522984094 24-25 21.49491535216964 27.407448678899538 31.177289948740807 19.920346020190014 26-27 21.37368034403916 27.569253563793346 30.291376734434454 20.765689357733038 28-29 21.373288264863927 28.735936739056868 30.077012604313698 19.813762391765515 30-31 20.16285730794311 28.733811257212196 30.512447482026268 20.59088395281843 32-33 19.941456388414267 29.486892174099662 30.438757232828994 20.132894204657077 34-35 21.03420168552774 28.93533995328067 30.66079786048585 19.36966050070574 36-37 20.15062031052671 29.389677174388563 31.30576810374002 19.153934411344707 38-39 20.752807551188024 29.7267358116 29.76629535314681 19.754161284065162 40-41 20.651915499910434 28.601163770722803 31.006899415307988 19.740021314058776 42-43 20.66485108764492 29.451527156196132 30.728272535786903 19.15534922037204 44-45 20.14347365725343 28.91231713691575 31.155715534780175 19.78849367105064 46-47 21.076236237797975 28.803851074240615 30.30306407306079 19.816848614900625 48-49 20.716209439254072 30.14665122969512 30.017562650783923 19.119576680266885 50-51 21.32114160053227 29.389923577415633 29.672979358142744 19.61595546390936 52-53 20.23052186988068 28.985924178029865 29.408636346188533 21.374917605900922 54-55 21.42518057895897 29.11352222468984 29.762875822605373 19.698421373745813 56-57 20.73605137989767 28.9760760493819 29.430083725983785 20.857788844736643 58-59 20.587481874055 28.77546534009691 30.217490698358475 20.419562087489616 60-61 20.768584060475796 29.16064713872976 30.39580370012358 19.674965100670864 62-63 20.260192474821032 29.27067310877318 29.96076908201633 20.508365334389453 64-65 21.10611735743104 29.019038364852044 29.835130451724645 20.039713825992266 66-67 20.85469221028384 30.140573833513766 28.939756872595996 20.064977083606394 68-69 21.082458596693517 30.202363414448374 28.784682164743803 19.93049582411431 70-71 20.60536215298592 31.07550213934563 28.74971720344343 19.569418504225023 72-73 20.23064178322909 31.406639945393326 28.696430771860058 19.666287499517527 74-75 20.932473494731973 30.49486821679823 28.599720910261915 19.97293737820788 76-77 21.315295580575803 29.582518652341495 29.143643671745213 19.958542095337492 78-79 21.187282903648796 29.395503859616824 29.677531744133173 19.739681492601203 80-81 20.381583148230202 30.051963709811396 29.431573273082208 20.134879868876197 82-83 19.518657759005304 29.91137187862809 29.713347418191095 20.85662294417551 84-85 19.701062786060493 29.46320409215353 30.080779004970733 20.75495411681525 86-87 19.64917828603535 29.667179887416196 30.763706740475776 19.91993508607267 88-89 19.984973316283828 30.125569821960628 30.235607254503105 19.65384960725244 90-91 20.32194006163624 29.509397245791792 29.663415603739786 20.50524708883218 92-93 19.682634126018556 30.405943993358857 29.60146349509997 20.309958385522613 94-95 19.313692821670877 30.906863443402944 29.602705556250626 20.176738178675553 96-97 19.834639601486888 31.35574099124996 29.02468866421274 19.78493074305041 98-99 20.29635578384092 30.4106452731717 28.70782271916496 20.585176223822423 100-101 20.223970093278894 30.266558020554264 28.367845402665353 21.14162648350149 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 118.0 1 79.5 2 68.5 3 146.0 4 159.0 5 97.5 6 70.0 7 73.0 8 81.5 9 76.0 10 80.0 11 92.0 12 90.5 13 86.0 14 86.0 15 90.5 16 115.5 17 146.0 18 164.5 19 237.0 20 336.5 21 498.5 22 751.0 23 1137.0 24 1652.5 25 2423.5 26 3659.0 27 5324.5 28 7186.5 29 9051.0 30 11246.5 31 13928.0 32 19422.5 33 30096.5 34 46325.5 35 70191.5 36 103843.5 37 145642.5 38 187539.5 39 205421.0 40 201945.5 41 182690.5 42 156405.0 43 146463.0 44 138413.5 45 128493.5 46 125612.0 47 111855.0 48 83543.5 49 62691.0 50 50399.0 51 40773.0 52 33276.0 53 26340.5 54 19669.0 55 13852.0 56 9603.0 57 6474.5 58 4627.0 59 3339.5 60 2411.0 61 1800.0 62 1301.0 63 916.5 64 646.5 65 453.0 66 315.5 67 227.5 68 176.5 69 132.5 70 90.5 71 63.5 72 55.0 73 40.5 74 27.0 75 21.5 76 18.0 77 13.0 78 10.5 79 8.0 80 5.0 81 3.0 82 1.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18448356981898323 2 0.0 3 0.0 4 0.008832099315718659 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0027032827344839827 12-13 0.0 14-15 0.0 16-17 3.9207917523050126E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0020635746064763226 40-41 0.006851067693501391 42-43 0.0025381967659658766 44-45 0.001093694541432451 46-47 0.007243146868731892 48-49 0.009286085729143452 50-51 0.005612922929615597 52-53 0.007139968138408077 54-55 0.020388117111986067 56-57 0.022864406639757653 58-59 0.028580508299697067 60-61 0.02705346309090459 62-63 0.011287753097425485 64-65 0.016426053867551527 66-67 0.02424700162609679 68-69 0.02804397890201322 70-71 0.03070599014436768 72-73 0.023339028799247208 74-75 0.03384262354621169 76-77 0.03153141998695821 78-79 0.021646897621936625 80-81 0.009554350427985372 82-83 0.001939760130087743 84-85 0.008130483949516711 86-87 0.028704322776085645 88-89 0.025691503850630217 90-91 0.022348512988138572 92-93 0.045666906041321016 94-95 0.04742094445682589 96-97 0.036174462851529934 98-99 0.043747781657298035 100-101 0.04331443098993801 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2422980.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.132448187216546 #Duplication Level Percentage of deduplicated Percentage of total 1 87.52376357514503 35.125429068296995 2 5.492547701849261 4.408587721205616 3 2.002189213913867 2.4105826466520632 4 0.938108076759919 1.5059429513830724 5 0.7066138316222332 1.4179071492974915 6 0.4636202755980579 1.1163730013389268 7 0.3077075362479284 0.8644339728700231 8 0.26979992545287373 0.8662185223321874 9 0.24314416173613781 0.8782173425609776 >10 1.597396131528502 12.662171365591703 >50 0.2153064604899073 6.033457160218045 >100 0.199394157653427 17.59313518223993 >500 0.03228165060102405 8.703453460749918 >1k 0.007920173684748375 4.847342468170872 >5k 1.0356385861652259E-4 0.2142557798442842 >10k+ 1.0356385856376983E-4 1.3524922072476127 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 32541 1.3430156253869203 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5155 0.21275454192770885 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 4042 0.16681937118754592 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4019 0.1658701268685668 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 3883 0.1602572039389512 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3203 0.1321925892908732 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3147 0.12988138573161975 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 3018 0.12455736324691084 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 2776 0.11456966215156543 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.905158111086349E-4 0.0 0.0 2 9.905158111086349E-4 0.0 0.0 3 0.0010317873032381613 0.0 0.0 4 0.0010317873032381613 0.0 0.0 5 0.0011556017796267406 0.0 0.0 6 0.00127941625601532 0.0 0.0 7 0.001361959240274373 0.0 0.0 8 0.0014857737166629522 0.0 0.0 9 0.0016508596851810581 0.0 0.0 10-11 0.003095361909714484 0.0 0.0 12-13 0.003941427498369776 0.0 4.127149212952645E-5 14-15 0.005489108453227018 0.0 4.127149212952645E-5 16-17 0.007263782614796655 0.0 4.127149212952645E-5 18-19 0.009120999760625345 0.0 4.127149212952645E-5 20-21 0.011432203319878827 0.0 8.25429842590529E-5 22-23 0.013846585609456124 0.0 8.25429842590529E-5 24-25 0.01675622580458774 0.0 8.25429842590529E-5 26-27 0.020181759651338435 0.0 8.25429842590529E-5 28-29 0.023669200736283418 0.0 8.25429842590529E-5 30-31 0.037577693583933834 0.0 8.25429842590529E-5 32-33 0.04145721384410932 0.0 8.25429842590529E-5 34-35 0.04558436305706197 0.0 8.25429842590529E-5 36-37 0.05059884935079943 0.0 8.25429842590529E-5 38-39 0.0559435075815731 0.0 8.25429842590529E-5 40-41 0.062278681623455415 0.0 8.25429842590529E-5 42-43 0.06826304798223676 0.0 8.25429842590529E-5 44-45 0.07540301612064483 0.0 1.2381447638857936E-4 46-47 0.0824398055287291 0.0 1.2381447638857936E-4 48-49 0.08918769449190667 0.0 1.2381447638857936E-4 50-51 0.09665783456735094 0.0 1.2381447638857936E-4 52-53 0.10482959000899719 0.0 1.650859685181058E-4 54-55 0.11306325268883771 0.0 1.650859685181058E-4 56-57 0.12294777505385929 0.0 1.650859685181058E-4 58-59 0.13289420465707516 0.0 1.650859685181058E-4 60-61 0.14385178581746444 0.0 1.650859685181058E-4 62-63 0.15520144615308423 0.0 1.650859685181058E-4 64-65 0.16778925125258978 0.0 1.650859685181058E-4 66-67 0.18109930746436206 0.0 1.650859685181058E-4 68-69 0.19525542926478962 0.0 1.8572171458286903E-4 70-71 0.2112687682110459 0.0 2.0635746064763227E-4 72-73 0.2282726229684108 0.0 2.0635746064763227E-4 74-75 0.24857819709613782 0.0 2.0635746064763227E-4 76-77 0.26958538659006676 0.0 2.0635746064763227E-4 78-79 0.29284187240505494 0.0 2.0635746064763227E-4 80-81 0.31791430387374225 0.0 2.476289527771587E-4 82-83 0.3458138325533021 0.0 2.8890044490668514E-4 84-85 0.3758594788235974 0.0 2.8890044490668514E-4 86-87 0.4125704710728112 0.0 2.8890044490668514E-4 88-89 0.4506640583083641 0.0 3.095361909714484E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 5520 0.0 68.31573 4 TCGGAAG 5550 0.0 68.28875 3 GATCGGA 5685 0.0 66.95027 1 ATCGGAA 5770 0.0 65.43809 2 GAAGAGC 5885 0.0 64.07071 6 CACGCGG 30 1.2911888E-4 63.435383 1 GGAAGAG 6575 0.0 57.346935 5 CGCGGCG 25 0.0049900436 57.091846 1 CGTTCGG 25 0.0049900436 57.091846 1 CGAGGCG 85 1.8189894E-12 55.972393 1 TTGTGTT 27215 0.0 49.001297 1 CGGACGG 50 2.8011476E-5 47.576538 1 CCGCCGA 70 7.933704E-8 47.494057 4 TCGGACG 75 1.3649878E-7 44.327785 3 GCCGTCT 4290 0.0 43.74034 50-51 CGTCTTC 4285 0.0 43.73685 52-53 GTCGGGC 55 4.9179987E-5 43.251396 1 ATGCCGT 4330 0.0 43.222992 48-49 TCGTATG 4355 0.0 43.189003 44-45 TGCCGGC 55 4.968616E-5 43.17641 2 >>END_MODULE