FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_35008-1.341000000357b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_35008-1.341000000357b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422980
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT325411.3430156253869203TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC51550.21275454192770885No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA40420.16681937118754592No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG40190.1658701268685668No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC38830.1602572039389512No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA32030.1321925892908732No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA31470.12988138573161975No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG30180.12455736324691084No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC27760.11456966215156543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA55200.068.315734
TCGGAAG55500.068.288753
GATCGGA56850.066.950271
ATCGGAA57700.065.438092
GAAGAGC58850.064.070716
CACGCGG301.2911888E-463.4353831
GGAAGAG65750.057.3469355
CGCGGCG250.004990043657.0918461
CGTTCGG250.004990043657.0918461
CGAGGCG851.8189894E-1255.9723931
TTGTGTT272150.049.0012971
CGGACGG502.8011476E-547.5765381
CCGCCGA707.933704E-847.4940574
TCGGACG751.3649878E-744.3277853
GCCGTCT42900.043.7403450-51
CGTCTTC42850.043.7368552-53
GTCGGGC554.9179987E-543.2513961
ATGCCGT43300.043.22299248-49
TCGTATG43550.043.18900344-45
TGCCGGC554.968616E-543.176412
Produced by FastQC (version 0.11.2)