##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_35005-1.3410000003578e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2652944 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66597410273266 33.0 33.0 33.0 33.0 33.0 2 32.68260958391885 33.0 33.0 33.0 33.0 33.0 3 32.73659677701452 33.0 33.0 33.0 33.0 33.0 4 36.68774614164491 37.0 37.0 37.0 37.0 37.0 5 36.63940098245572 37.0 37.0 37.0 37.0 37.0 6 36.548739815088446 37.0 37.0 37.0 37.0 37.0 7 36.518102907562316 37.0 37.0 37.0 37.0 37.0 8 36.45645328359739 37.0 37.0 37.0 37.0 37.0 9 36.45515321846221 37.0 37.0 37.0 37.0 37.0 10-11 36.41765487699703 37.0 37.0 37.0 37.0 37.0 12-13 36.40256277554295 37.0 37.0 37.0 37.0 37.0 14-15 38.260389966769 40.0 37.0 40.0 37.0 40.0 16-17 38.25499200133889 40.0 37.0 40.0 37.0 40.0 18-19 38.258380124118716 40.0 37.0 40.0 37.0 40.0 20-21 38.19335726649338 40.0 37.0 40.0 37.0 40.0 22-23 38.10761158169943 40.0 37.0 40.0 37.0 40.0 24-25 38.071427440609376 40.0 37.0 40.0 37.0 40.0 26-27 37.98646239800011 40.0 37.0 40.0 35.0 40.0 28-29 37.88321445910657 40.0 37.0 40.0 33.0 40.0 30-31 37.842832340222785 40.0 37.0 40.0 33.0 40.0 32-33 37.72398230041795 40.0 37.0 40.0 33.0 40.0 34-35 37.65492863776996 40.0 37.0 40.0 33.0 40.0 36-37 37.58803088191836 40.0 37.0 40.0 33.0 40.0 38-39 37.49303603845388 40.0 37.0 40.0 33.0 40.0 40-41 37.40068090393163 40.0 37.0 40.0 33.0 40.0 42-43 37.271993302534845 40.0 37.0 40.0 33.0 40.0 44-45 37.15523037802532 40.0 37.0 40.0 33.0 40.0 46-47 37.09169982479841 40.0 37.0 40.0 33.0 40.0 48-49 36.88537771622771 37.0 37.0 40.0 33.0 40.0 50-51 36.73689286317389 37.0 37.0 40.0 33.0 40.0 52-53 36.61455952330694 37.0 37.0 40.0 33.0 40.0 54-55 36.367517934792446 37.0 37.0 40.0 33.0 40.0 56-57 36.23254448642715 37.0 37.0 40.0 33.0 40.0 58-59 36.00612168971527 37.0 37.0 40.0 33.0 40.0 60-61 35.74197344534977 37.0 37.0 40.0 30.0 40.0 62-63 35.43312674523096 37.0 33.0 40.0 27.0 40.0 64-65 35.182230005608865 37.0 33.0 37.0 27.0 40.0 66-67 34.867703012200785 37.0 33.0 37.0 27.0 40.0 68-69 34.58453156191763 37.0 33.0 37.0 27.0 40.0 70-71 34.182571513005925 37.0 33.0 37.0 27.0 40.0 72-73 33.930605960774145 37.0 33.0 37.0 27.0 37.0 74-75 33.55820684492397 37.0 33.0 37.0 27.0 37.0 76-77 30.932511391118695 33.0 30.0 35.0 24.5 37.0 78-79 33.23475580336411 35.0 33.0 37.0 27.0 37.0 80-81 33.61872074947681 37.0 33.0 37.0 27.0 37.0 82-83 33.611871000669446 37.0 33.0 37.0 27.0 37.0 84-85 33.48780807284285 37.0 33.0 37.0 27.0 37.0 86-87 33.35888507258352 37.0 33.0 37.0 27.0 37.0 88-89 33.14485661966479 37.0 33.0 37.0 27.0 37.0 90-91 32.907472792490154 37.0 33.0 37.0 27.0 37.0 92-93 32.90751708290865 33.0 33.0 37.0 27.0 37.0 94-95 32.8372766255149 33.0 33.0 37.0 27.0 37.0 96-97 32.66806479895543 33.0 33.0 37.0 27.0 37.0 98-99 32.50933359317045 33.0 33.0 37.0 27.0 37.0 100-101 30.90688683967698 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.19700668204706773 2306 2 0.19837037999000273 2306 3 0.14289929355232545 2306 4 0.13175662489665285 2306 5 0.23441168515895328 2306 6 0.14504648194424874 2306 7 0.22967571214675075 2306 8 0.21370744855450852 2306 9 0.2511436355420429 2306 10-11 0.20261510861708132 2306 12-13 0.23513025276398736 2306 14-15 0.44864091130515504 2306 16-17 0.3952139382615698 2306 18-19 0.45075515493910245 2306 20-21 0.4678248879906448 2306 22-23 0.48114876607456836 2306 24-25 0.549836420146768 2306 26-27 0.498936454168728 2306 28-29 0.40331983976131625 2306 30-31 0.43328543273160847 2306 32-33 0.4899763335435807 2306 34-35 0.43779506313788374 2306 36-37 0.5601140100019606 2306 38-39 0.47051830601075295 2306 40-41 0.5291357418507587 2306 42-43 0.40135003016863635 2306 44-45 0.5074660388679888 2306 46-47 0.5313468978839424 2306 48-49 0.622402913564251 2306 50-51 0.5354912286885423 2306 52-53 0.3526283281298248 2306 54-55 0.641325694114748 2306 56-57 0.6613194122740111 2306 58-59 0.6183564912474253 2306 60-61 0.5389891857892195 2306 62-63 0.47297547326083134 2306 64-65 0.4234041703529954 2306 66-67 0.484941445709147 2306 68-69 0.6234563516974845 2306 70-71 0.6299088240553985 2306 72-73 0.5430004583341415 2306 74-75 0.578686416254925 2306 76-77 0.06995033019662245 2306 78-79 0.632098163766706 2306 80-81 0.593679172839515 2306 82-83 0.660103755638346 2306 84-85 0.6683999084269772 2306 86-87 0.5753288890491461 2306 88-89 0.7623530263836784 2306 90-91 0.8547143621545601 2306 92-93 0.5298497546044842 2306 94-95 0.5283759908875467 2306 96-97 0.6559044927361981 2306 98-99 0.5355300060272867 2306 100-101 0.2463928181661572 2307 1 0.16277721350976293 2307 2 0.17497844009579921 2307 3 0.1644393342702557 2307 4 0.14024392822820175 2307 5 0.20064646917504092 2307 6 0.16317490527443113 2307 7 0.07829913943798061 2307 8 0.16775964928198306 2307 9 0.18418819526877428 2307 10-11 0.2443303301379558 2307 12-13 0.21665087154023155 2307 14-15 0.3971124981970391 2307 16-17 0.4746694850147861 2307 18-19 0.34056097811425445 2307 20-21 0.34885825454572483 2307 22-23 0.3627833183902922 2307 24-25 0.44384713628827654 2307 26-27 0.4864146257803128 2307 28-29 0.4713266629960913 2307 30-31 0.4435799136631857 2307 32-33 0.3955299014735232 2307 34-35 0.4397589792146448 2307 36-37 0.35357422483589573 2307 38-39 0.3742319005180903 2307 40-41 0.49532367469172556 2307 42-43 0.38858627934873624 2307 44-45 0.4863382884910479 2307 46-47 0.41437612449881556 2307 48-49 0.555672358931524 2307 50-51 0.4457607933133545 2307 52-53 0.5111192517794407 2307 54-55 0.5137077782253883 2307 56-57 0.48182169946319675 2307 58-59 0.5296184489450724 2307 60-61 0.5455775258094917 2307 62-63 0.4153383057976967 2307 64-65 0.36846797313510393 2307 66-67 0.2739395120333725 2307 68-69 0.31711930434372704 2307 70-71 0.36918459007210913 2307 72-73 0.37585314120279634 2307 74-75 0.396408725080839 2307 76-77 0.05688162081871795 2307 78-79 0.42208287324682914 2307 80-81 0.4572939113673655 2307 82-83 0.2646694026530767 2307 84-85 0.4739796704230059 2307 86-87 0.4485189429705869 2307 88-89 0.5434990487189069 2307 90-91 0.6716020811838064 2307 92-93 0.3706643687637623 2307 94-95 0.3626761621072916 2307 96-97 0.21898321122878173 2307 98-99 0.14817854554838306 2307 100-101 0.3327682834835102 2201 1 0.14493934950259302 2201 2 0.14862186033092684 2201 3 0.11108268188549886 2201 4 0.14925000409176903 2201 5 0.1744532841476456 2201 6 0.15986949025179342 2201 7 0.16577422678356868 2201 8 0.143415447172778 2201 9 0.17659618991103798 2201 10-11 0.20352340220108545 2201 12-13 0.15233466843048404 2201 14-15 0.2630507470895864 2201 16-17 0.31544212022897256 2201 18-19 0.2049193411007053 2201 20-21 0.29646179839603093 2201 22-23 0.37062848473016885 2201 24-25 0.31829260279033633 2201 26-27 0.30830810854922674 2201 28-29 0.36402251478698133 2201 30-31 0.35105209621342937 2201 32-33 0.3582161916464415 2201 34-35 0.3278694905393138 2201 36-37 0.3764016799931156 2201 38-39 0.26235370826905324 2201 40-41 0.3212804416545154 2201 42-43 0.4017052629931257 2201 44-45 0.3772309482346117 2201 46-47 0.4155808076743952 2201 48-49 0.5257465119012679 2201 50-51 0.43313885242593386 2201 52-53 0.32005654047575405 2201 54-55 0.38183975276000837 2201 56-57 0.417922455516873 2201 58-59 0.4891387139269554 2201 60-61 0.42941455872243495 2201 62-63 0.3990649968323652 2201 64-65 0.2685379706337585 2201 66-67 0.32038718940744104 2201 68-69 0.4228141152496221 2201 70-71 0.42837544319436205 2201 72-73 0.4040088785770237 2201 74-75 0.43543081766311786 2201 76-77 0.2502193516894309 2201 78-79 0.5788879614799924 2201 80-81 0.4417546683893008 2201 82-83 0.48474980900167 2201 84-85 0.4498704743251096 2201 86-87 0.44944968780852435 2201 88-89 0.5316311442866706 2201 90-91 0.7152756790148942 2201 92-93 0.4455879161643779 2201 94-95 0.24635726399298363 2201 96-97 0.3880503684830998 2201 98-99 0.39076405546099835 2201 100-101 0.4167098083396823 1101 1 0.12130528832847176 1101 2 0.1699416676812433 1101 3 0.0932086551179836 1101 4 0.013417868276704326 1101 5 0.1078593219152637 1101 6 0.11776008316271458 1101 7 0.0938719306205158 1101 8 0.13165246135298503 1101 9 0.048322925914298764 1101 10-11 0.1519046760874545 1101 12-13 0.19882426168387468 1101 14-15 0.3152400721671569 1101 16-17 0.2569855932708762 1101 18-19 0.25232172202871084 1101 20-21 0.23650339580006374 1101 22-23 0.2323586454649913 1101 24-25 0.33404636800393916 1101 26-27 0.35502326759444713 1101 28-29 0.3461840437874031 1101 30-31 0.2667004456862401 1101 32-33 0.21359922113440177 1101 34-35 0.23435725195954404 1101 36-37 0.37518083714505934 1101 38-39 0.31438801019839246 1101 40-41 0.4184580532398954 1101 42-43 0.3611864787386949 1101 44-45 0.32213828136997336 1101 46-47 0.5084775047995649 1101 48-49 0.476070268501843 1101 50-51 0.2661271525871598 1101 52-53 0.35121334880798116 1101 54-55 0.525905182199736 1101 56-57 0.23681900928117727 1101 58-59 0.4321095377171247 1101 60-61 0.3872050265908342 1101 62-63 0.4371412420522489 1101 64-65 0.4240335839053273 1101 66-67 0.37445954795521175 1101 68-69 0.49153715686636446 1101 70-71 0.4276580700203638 1101 72-73 0.3896619839893489 1101 74-75 0.5284279242769472 1101 76-77 0.10583591146123794 1101 78-79 0.36053714608667065 1101 80-81 0.5771720817270918 1101 82-83 0.4518618614398804 1101 84-85 0.5489359549651383 1101 86-87 0.36374834033025394 1101 88-89 0.4891869440866472 1101 90-91 0.5277997858255574 1101 92-93 0.29929641120534 1101 94-95 0.3113688523288758 1101 96-97 0.20683684158985471 1101 98-99 0.36394874861756676 1101 100-101 0.48859361550340097 2304 1 0.09771679119036492 2304 2 0.09478542060087136 2304 3 0.07826506479944584 2304 4 0.10298761485809393 2304 5 0.13179976162215468 2304 6 0.10907599098496945 2304 7 0.13689219289755528 2304 8 0.08210874400858614 2304 9 0.16042298354942375 2304 10-11 0.13234129531262084 2304 12-13 0.18811613690826334 2304 14-15 0.2977797296544722 2304 16-17 0.2575002639242925 2304 18-19 0.25548158577250746 2304 20-21 0.22934653481573974 2304 22-23 0.3072599155966955 2304 24-25 0.24647843084273546 2304 26-27 0.2653944708552558 2304 28-29 0.1681468202369274 2304 30-31 0.11801341823489508 2304 32-33 0.09077099251032905 2304 34-35 0.34095739059588936 2304 36-37 0.31249660019918224 2304 38-39 0.2730138050851778 2304 40-41 0.30805230881151857 2304 42-43 0.2687253606824669 2304 44-45 0.40537315242021066 2304 46-47 0.42299001989544394 2304 48-49 0.40526767798683494 2304 50-51 0.3060560347903518 2304 52-53 0.2583963877885225 2304 54-55 0.4192588737779488 2304 56-57 0.42793670449999865 2304 58-59 0.38489136253208756 2304 60-61 0.4959219002233155 2304 62-63 0.5250574028014796 2304 64-65 0.3194725352035519 2304 66-67 0.3895645964625203 2304 68-69 0.35663339773876146 2304 70-71 0.44798139276458215 2304 72-73 0.32905112416644755 2304 74-75 0.4154477380993242 2304 76-77 0.35918494433575887 2304 78-79 0.4644849257916661 2304 80-81 0.5909473678482371 2304 82-83 0.5328027563137283 2304 84-85 0.570984703364843 2304 86-87 0.5252514289891153 2304 88-89 0.6405256955214114 2304 90-91 0.6887536445220661 2304 92-93 0.4656061464266088 2304 94-95 0.42581758236951117 2304 96-97 0.4564220845291018 2304 98-99 0.2697289154927631 2304 100-101 0.4789025866101895 2202 1 0.0947027794322608 2202 2 0.12497695497853556 2202 3 0.09759624804806322 2202 4 0.051988792507046355 2202 5 0.15118798005909184 2202 6 0.09392778547373126 2202 7 0.049644358659854504 2202 8 0.14106967865017594 2202 9 0.14786519711402946 2202 10-11 0.1927346644527006 2202 12-13 0.1470101956633627 2202 14-15 0.2322114812629934 2202 16-17 0.24407690466066612 2202 18-19 0.15857684287946938 2202 20-21 0.1826385462970066 2202 22-23 0.2615533628324229 2202 24-25 0.37356934510318496 2202 26-27 0.2835092723038457 2202 28-29 0.13414814447482115 2202 30-31 0.19255611094615688 2202 32-33 0.21553665875709527 2202 34-35 0.407671535509877 2202 36-37 0.4439295948342945 2202 38-39 0.3855607463220778 2202 40-41 0.40854975780631264 2202 42-43 0.4043382684776802 2202 44-45 0.44422407554750976 2202 46-47 0.4551347966554644 2202 48-49 0.5151772950900408 2202 50-51 0.4966387760785622 2202 52-53 0.5337016665058698 2202 54-55 0.5184131866511947 2202 56-57 0.5561270358608255 2202 58-59 0.5285795165958831 2202 60-61 0.5586548187377431 2202 62-63 0.3498979128356652 2202 64-65 0.3657553136242342 2202 66-67 0.4958993841647725 2202 68-69 0.5341468235667151 2202 70-71 0.4741623318770465 2202 72-73 0.26325814010752424 2202 74-75 0.2695337645292639 2202 76-77 0.08772945570338564 2202 78-79 0.45642704546102664 2202 80-81 0.48880262343654124 2202 82-83 0.4993176526280081 2202 84-85 0.46720373006531446 2202 86-87 0.48130309231397916 2202 88-89 0.5937397305362495 2202 90-91 0.6380460421400755 2202 92-93 0.5920535845847112 2202 94-95 0.4248283197970437 2202 96-97 0.3296068119698532 2202 98-99 0.1951492775998105 2202 100-101 0.3830492491067581 1102 1 0.09097005481666542 1102 2 0.03330983675291321 1102 3 0.052781096756284285 1102 4 0.06366830378845378 1102 5 0.07737167879718498 1102 6 0.012884588286645737 1102 7 0.0892793558292766 1102 8 0.04094221698264988 1102 9 0.07910082350440462 1102 10-11 0.046963379794334514 1102 12-13 0.08379064054086172 1102 14-15 0.09480038345727593 1102 16-17 0.10662805489928218 1102 18-19 0.09023644651323082 1102 20-21 0.11901665621605417 1102 22-23 0.14407733166297731 1102 24-25 0.13996105711695606 1102 26-27 0.15871969470754266 1102 28-29 0.139948358196456 1102 30-31 0.11920878009892277 1102 32-33 0.12299197028639952 1102 34-35 0.09339163046905696 1102 36-37 0.18442981150123927 1102 38-39 0.0981101960954689 1102 40-41 0.1317534766343087 1102 42-43 0.17999564474605023 1102 44-45 0.1179640267335742 1102 46-47 0.0847899829849581 1102 48-49 0.09913764447137652 1102 50-51 0.13589158351414454 1102 52-53 0.09441486603780902 1102 54-55 0.2541284243005677 1102 56-57 0.1308831492774658 1102 58-59 0.1342855619694987 1102 60-61 0.2508793152983202 1102 62-63 0.27249257220319123 1102 64-65 0.2995310608241155 1102 66-67 0.19596579180746687 1102 68-69 0.13604773216841437 1102 70-71 0.23540895677898988 1102 72-73 0.192061368941161 1102 74-75 0.20163858088145759 1102 76-77 -0.1583073618913744 1102 78-79 0.27554408254435714 1102 80-81 0.28392975721531144 1102 82-83 0.1850857511515045 1102 84-85 0.1827757771504679 1102 86-87 0.14617556474325255 1102 88-89 0.1568450534480874 1102 90-91 0.36809116386328355 1102 92-93 0.2357970034180923 1102 94-95 0.14627933899406997 1102 96-97 -0.0028925611361430015 1102 98-99 -0.046526212772867837 1102 100-101 0.06637063036620106 2305 1 0.08958453874657835 2305 2 0.1420095446374745 2305 3 0.06609120786230704 2305 4 0.06789726573060051 2305 5 0.08717494081000154 2305 6 0.05627427351894454 2305 7 0.03593208168458517 2305 8 0.1122188196854097 2305 9 0.09151627463261036 2305 10-11 0.03816254185066015 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-1.3673378524362931 1213 96-97 -1.5213058037599119 1213 98-99 -1.442592076305658 1213 100-101 -1.2920714776333782 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 8.0 9 12.0 10 14.0 11 25.0 12 42.0 13 96.0 14 287.0 15 645.0 16 1273.0 17 1933.0 18 2793.0 19 3679.0 20 4658.0 21 5982.0 22 7557.0 23 9623.0 24 12117.0 25 15160.0 26 19059.0 27 25079.0 28 33003.0 29 43371.0 30 49221.0 31 59077.0 32 78971.0 33 112200.0 34 171498.0 35 297031.0 36 593124.0 37 899611.0 38 205351.0 39 443.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.404384988004104 26.05830311228634 21.877545563822206 14.659766335887348 2 32.61406950165552 22.56670325494997 31.20469184423041 13.6145353991641 3 37.71070931010983 18.869075261294622 26.31359727155945 17.106618157036106 4 31.877476241277787 18.64225640948313 31.646241013906533 17.83402633533255 5 31.218337062523748 17.31992835129577 33.785108166625456 17.67662641955503 6 27.064310441532125 17.89325368345506 37.22947035444397 17.812965520568845 7 33.86630098486813 18.72105102859314 27.721203312244814 19.69144467429392 8 31.100392620424707 26.687823037350206 23.38537865857704 18.826405683648055 9 29.072306087124346 25.47524561392928 25.669143411998142 19.783304886948237 10-11 24.077570945461392 28.207861546258517 30.57858747435119 17.135980033928902 12-13 23.766257410635127 27.038037742221473 30.055666459601106 19.140038387542294 14-15 22.791660886924113 27.763477103172928 30.725733373942305 18.71912863596065 16-17 22.489033835381445 27.964973107123324 31.324858185261366 18.22113487223386 18-19 21.56106574432027 27.893672086557427 31.709301063271596 18.835961105850707 20-21 22.089082920709973 28.12961751171529 31.206840400702013 18.574459166872728 22-23 21.4733895626896 28.634697905421298 30.92458792948513 18.96732460240397 24-25 21.72584494810294 27.43154397529688 31.408616239166754 19.43399483743343 26-27 21.57188391462466 27.699284266837143 30.99660226525701 19.732229553281186 28-29 21.420712235162146 28.698042627360397 30.470545175472985 19.410699962004475 30-31 20.57427522028358 28.941451459209087 30.723415194591368 19.760858125915963 32-33 20.31335753788998 29.580722397457315 30.96989608525472 19.136023979397983 34-35 21.09105959266385 28.963351657630167 31.04471862202896 18.900870127677024 36-37 20.488973758963628 29.16659756104916 31.76974334927537 18.574685330711844 38-39 21.111096242599327 29.21241011434912 30.41329164537695 19.263201997674607 40-41 21.039848612215817 28.557933345647413 30.8486159854342 19.553602056702566 42-43 20.900506563920217 29.07125047613465 30.8032026404576 19.22504031948753 44-45 20.500586243141726 28.53778087793798 31.355800113045696 19.6058327658746 46-47 21.123175676388854 28.419320376784363 30.753282236942297 19.704221709884486 48-49 20.74292131426006 29.935288988610548 30.056447785421515 19.265341911707885 50-51 21.451373502877622 29.12553634418006 29.6331137224006 19.78997643054172 52-53 20.86073161735117 28.88821914782172 29.496320162366345 20.75472907246077 54-55 21.791650298176705 28.967062199759162 29.671428797634192 19.569858704429937 56-57 21.2262406333946 29.183543051039162 29.498053631179605 20.092162684386636 58-59 21.00834316153338 29.30452539596838 30.010968120435923 19.676163322062322 60-61 21.065698841256296 29.532242016175587 29.928091758415153 19.473967384152964 62-63 20.634570883084223 29.331548216714864 29.70594394449828 20.32793695570263 64-65 21.110907323480273 28.97747011500471 29.704225397246077 20.207397164268937 66-67 21.200555660599125 29.170334027877665 29.46722298077715 20.161887330746055 68-69 21.30838214418351 29.68990421322314 29.248278345326316 19.753435297267032 70-71 20.614261647794113 30.707313541786064 28.972096874415577 19.706327936004246 72-73 20.432506288280848 30.439671620064363 28.997971425011247 20.12985066664354 74-75 20.923949724634763 29.739426736558084 29.02307366877894 20.31354987002821 76-77 20.84603519154685 29.22771570913037 29.317642037410106 20.60860706191267 78-79 20.687502933652237 29.317426544646843 29.488855514634704 20.506215007066213 80-81 20.14600204885395 29.707232529844564 29.465761803293056 20.68100361800843 82-83 19.582576219193285 29.45589746131721 29.636982892063244 21.324543427426264 84-85 19.751969942056206 29.28167183929992 29.988996295532587 20.977361923111285 86-87 20.00806117595515 29.323847186798524 30.540658697614848 20.127432939631483 88-89 20.255646524990624 29.890585323276635 29.871846834533866 19.98192131719888 90-91 20.488767656279162 29.50297562382535 29.369473134556255 20.638783585339233 92-93 19.919914654068414 30.06505779479266 29.621000257938395 20.394027293200534 94-95 19.554561577707076 30.42211417605073 29.67217460790943 20.351149638332767 96-97 19.897433693037495 30.89081784384515 29.213675281354114 19.99807318176325 98-99 20.40204323047922 30.186350885541913 28.83436612303955 20.57723976093932 100-101 20.422034771043528 30.011061919788922 28.281729581217625 21.28517372794993 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 372.0 1 230.5 2 94.0 3 153.0 4 197.0 5 150.5 6 117.5 7 123.0 8 122.5 9 136.0 10 149.5 11 140.5 12 141.0 13 147.5 14 154.0 15 160.0 16 158.0 17 175.5 18 228.5 19 271.0 20 342.5 21 481.5 22 717.0 23 1058.0 24 1601.5 25 2538.5 26 4285.0 27 7152.5 28 10038.0 29 12914.5 30 16561.0 31 19929.5 32 25504.5 33 36541.5 34 53404.0 35 77972.5 36 113696.5 37 154069.5 38 187539.0 39 204735.5 40 204872.0 41 190420.0 42 171759.0 43 162764.5 44 149977.5 45 134750.0 46 126344.0 47 112375.5 48 93491.5 49 77685.0 50 63955.5 51 52680.0 52 42990.5 53 34128.0 54 27806.5 55 21858.5 56 15382.5 57 10112.0 58 6912.5 59 4947.5 60 3653.5 61 2710.5 62 1914.5 63 1384.5 64 1016.5 65 710.5 66 514.0 67 397.5 68 312.5 69 235.0 70 170.0 71 128.5 72 84.5 73 41.5 74 33.5 75 30.0 76 24.0 77 17.0 78 9.0 79 9.0 80 8.5 81 3.0 82 1.0 83 1.5 84 0.5 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18677363713670547 2 0.0 3 0.0 4 0.008820389725527565 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0016396878335916626 12-13 0.0 14-15 0.0 16-17 4.1463370504616763E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0022427914045679066 40-41 0.005804871870646346 42-43 0.002657425109614074 44-45 0.0013004420749175257 46-47 0.006784915173482743 48-49 0.008556531913225458 50-51 0.005522167071751231 52-53 0.006690680240517704 54-55 0.018470046861147466 56-57 0.021447870742842667 58-59 0.026838108908442848 60-61 0.025443431900560285 62-63 0.010158525773631105 64-65 0.0150964362609991 66-67 0.022823700764132225 68-69 0.02634808725702465 70-71 0.0289489714068597 72-73 0.022220597193155982 74-75 0.03128599774439264 76-77 0.029137441272789777 78-79 0.020109734694739128 80-81 0.00910309452442268 82-83 0.0019789335922657998 84-85 0.007576488610389062 86-87 0.027346977546454054 88-89 0.024463388597723887 90-91 0.021070931010982518 92-93 0.04327268121754549 94-95 0.04511968590366024 96-97 0.03405650477356477 98-99 0.04014408144310622 100-101 0.039710600751467054 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2652944.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.36887621962196 #Duplication Level Percentage of deduplicated Percentage of total 1 87.16061715613438 40.41539868138653 2 5.464289130108342 5.06745892604439 3 2.1606211036528515 3.0055671753834616 4 1.0400616383764512 1.929059574826195 5 0.7660136880976393 1.7759596942967768 6 0.558996367552726 1.5552020024562392 7 0.36255366727773075 1.176784428467977 8 0.27540234759429383 1.021607789295449 9 0.18054409844904354 0.7534464257850246 >10 1.67942401505565 15.360409374800607 >50 0.18898549386228058 6.081275101243603 >100 0.14508873753634083 14.640294917783564 >500 0.01485448266632944 4.461466964429229 >1k 0.002304412510290781 1.5799920170673578 >5k 1.624407515918843E-4 0.3934442111330096 >10k+ 8.12203740693413E-5 0.7826327156007682 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 20781 0.7833184567785825 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5366 0.20226586011615777 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5081 0.19152307775814342 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.146337050461676E-4 0.0 0.0 2 4.146337050461676E-4 0.0 0.0 3 5.654095977902286E-4 0.0 0.0 4 6.031035709762437E-4 0.0 0.0 5 6.40797544162259E-4 0.0 0.0 6 6.784915173482742E-4 0.0 0.0 7 7.915734369063199E-4 0.0 0.0 8 8.292674100923352E-4 0.0 0.0 9 0.0011308191955804571 0.0 0.0 10-11 0.001771616739742716 0.0 0.0 12-13 0.002713966069393097 0.0 0.0 14-15 0.0039578671845315995 0.0 0.0 16-17 0.0053525441924141635 0.0 0.0 18-19 0.006860303119854773 0.0 0.0 20-21 0.008801542738934558 0.0 0.0 22-23 0.011025487156909456 0.0 0.0 24-25 0.013550983360372477 0.0 0.0 26-27 0.01660419518843971 0.0 0.0 28-29 0.020109734694739128 0.0 0.0 30-31 0.030192872521998204 0.0 0.0 32-33 0.03426382162608785 0.0 0.0 34-35 0.038975568274339754 0.0 0.0 36-37 0.04361192697621963 0.0 0.0 38-39 0.04847444951721559 0.0 0.0 40-41 0.05399661658896682 0.0 0.0 42-43 0.059386854754567 0.0 0.0 44-45 0.06558751334366651 0.0 0.0 46-47 0.07278706222219541 0.0 0.0 48-49 0.0798923761677593 0.0 0.0 50-51 0.08743117080496234 0.0 0.0 52-53 0.09568615093269968 0.0 0.0 54-55 0.10399767202021604 0.0 0.0 56-57 0.11266728585299954 0.0 0.0 58-59 0.12265618874729356 0.0 0.0 60-61 0.13469941318022544 0.0 0.0 62-63 0.14736458817072656 0.0 0.0 64-65 0.16108519441043612 0.0 0.0 66-67 0.17571045600661 0.0 0.0 68-69 0.19227695722186372 0.0 0.0 70-71 0.2113124136808014 0.0 0.0 72-73 0.23292990730298113 0.0 0.0 74-75 0.2570917441152169 0.0 0.0 76-77 0.28293096273423035 0.0 0.0 78-79 0.3131803762160076 0.0 0.0 80-81 0.34757612674824645 0.0 0.0 82-83 0.38331001332858894 0.0 0.0 84-85 0.4267523174254715 0.0 0.0 86-87 0.4767533728567207 0.0 0.0 88-89 0.5301280388881182 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAATCG 20 0.0020755413 71.23992 4 CGACGTA 20 0.0020758517 71.237236 5 ACGCGGT 25 0.0049881726 57.09741 1 CGAGGCG 160 0.0 56.50264 1 TCGGAAG 4490 0.0 50.8781 3 CGGAAGA 4510 0.0 50.441864 4 GAAGAGC 4625 0.0 49.288464 6 GATCGGA 4870 0.0 47.38577 1 ATCGGAA 4975 0.0 45.822662 2 ATGCGCC 85 7.1195245E-9 44.78228 1 AGACGGC 110 3.274181E-11 43.255608 1 CGCGTTG 45 9.635848E-4 42.214657 5 GCCGTCT 2775 0.0 41.510178 50-51 TATGCCG 2735 0.0 41.07442 48-49 ATGCCGT 2800 0.0 40.884308 48-49 TCGTATG 2850 0.0 40.415142 44-45 CGTATGC 2800 0.0 40.37347 46-47 TGCCGTC 2805 0.0 40.30417 50-51 TGACGGC 205 0.0 39.383186 6 CGTCTTC 2930 0.0 39.31499 52-53 >>END_MODULE