FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_35005-1.3410000003578e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_35005-1.3410000003578e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2652944
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT207810.7833184567785825TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC53660.20226586011615777No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG50810.19152307775814342No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAATCG200.002075541371.239924
CGACGTA200.002075851771.2372365
ACGCGGT250.004988172657.097411
CGAGGCG1600.056.502641
TCGGAAG44900.050.87813
CGGAAGA45100.050.4418644
GAAGAGC46250.049.2884646
GATCGGA48700.047.385771
ATCGGAA49750.045.8226622
ATGCGCC857.1195245E-944.782281
AGACGGC1103.274181E-1143.2556081
CGCGTTG459.635848E-442.2146575
GCCGTCT27750.041.51017850-51
TATGCCG27350.041.0744248-49
ATGCCGT28000.040.88430848-49
TCGTATG28500.040.41514244-45
CGTATGC28000.040.3734746-47
TGCCGTC28050.040.3041750-51
TGACGGC2050.039.3831866
CGTCTTC29300.039.3149952-53
Produced by FastQC (version 0.11.2)