##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_34344-1.34100000035857.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4816329 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.657165654588795 33.0 33.0 33.0 33.0 33.0 2 32.679310528828076 33.0 33.0 33.0 33.0 33.0 3 32.73499194095752 33.0 33.0 33.0 33.0 33.0 4 36.677792152487925 37.0 37.0 37.0 37.0 37.0 5 36.64102078574782 37.0 37.0 37.0 37.0 37.0 6 36.5321951635779 37.0 37.0 37.0 37.0 37.0 7 36.48591925510072 37.0 37.0 37.0 37.0 37.0 8 36.42716517081786 37.0 37.0 37.0 37.0 37.0 9 36.438592961568865 37.0 37.0 37.0 37.0 37.0 10-11 36.39682152112117 37.0 37.0 37.0 37.0 37.0 12-13 36.374777242169294 37.0 37.0 37.0 37.0 37.0 14-15 38.212016351042465 40.0 37.0 40.0 37.0 40.0 16-17 38.20671324571058 40.0 37.0 40.0 37.0 40.0 18-19 38.21173854610015 40.0 37.0 40.0 37.0 40.0 20-21 38.16473282867512 40.0 37.0 40.0 37.0 40.0 22-23 38.046298955075535 40.0 37.0 40.0 35.0 40.0 24-25 38.03000106512657 40.0 37.0 40.0 35.0 40.0 26-27 37.952816865293045 40.0 37.0 40.0 33.0 40.0 28-29 37.82427965365323 40.0 37.0 40.0 33.0 40.0 30-31 37.77070368324091 40.0 37.0 40.0 33.0 40.0 32-33 37.631845540452076 40.0 37.0 40.0 33.0 40.0 34-35 37.546015128949875 40.0 37.0 40.0 33.0 40.0 36-37 37.48810847431727 40.0 37.0 40.0 33.0 40.0 38-39 37.36808469687183 40.0 37.0 40.0 33.0 40.0 40-41 37.222194019553065 40.0 37.0 40.0 33.0 40.0 42-43 37.086787364401395 40.0 37.0 40.0 33.0 40.0 44-45 36.9702964643819 37.0 37.0 40.0 33.0 40.0 46-47 36.9458061315994 37.0 37.0 40.0 33.0 40.0 48-49 36.73531905731523 37.0 37.0 40.0 33.0 40.0 50-51 36.592528562729 37.0 37.0 40.0 33.0 40.0 52-53 36.4627058284432 37.0 37.0 40.0 33.0 40.0 54-55 36.207095071786 37.0 37.0 40.0 33.0 40.0 56-57 36.067215507910696 37.0 37.0 40.0 33.0 40.0 58-59 35.86456645715025 37.0 37.0 40.0 27.0 40.0 60-61 35.59981710136496 37.0 35.0 40.0 27.0 40.0 62-63 35.309920792371116 37.0 33.0 40.0 27.0 40.0 64-65 35.049975718020924 37.0 33.0 37.0 27.0 40.0 66-67 34.650227382722406 37.0 33.0 37.0 27.0 40.0 68-69 34.3797979747646 37.0 33.0 37.0 27.0 40.0 70-71 33.953188725271886 37.0 33.0 37.0 27.0 40.0 72-73 33.56835984418838 37.0 33.0 37.0 27.0 37.0 74-75 33.11354820237571 37.0 33.0 37.0 27.0 37.0 76-77 30.520579678007877 33.0 30.0 35.0 22.0 37.0 78-79 32.74959466016545 35.0 33.0 37.0 27.0 37.0 80-81 33.102149894660435 37.0 33.0 37.0 27.0 37.0 82-83 33.077822549082505 37.0 33.0 37.0 27.0 37.0 84-85 32.94424643748381 37.0 33.0 37.0 27.0 37.0 86-87 32.81141311152125 37.0 33.0 37.0 27.0 37.0 88-89 32.60129976585902 37.0 33.0 37.0 24.5 37.0 90-91 32.3500468925607 33.0 33.0 37.0 22.0 37.0 92-93 32.32963902590541 33.0 33.0 37.0 22.0 37.0 94-95 32.2515420977263 33.0 33.0 37.0 22.0 37.0 96-97 32.085445367208095 33.0 33.0 37.0 22.0 37.0 98-99 31.923246522403268 33.0 33.0 37.0 22.0 37.0 100-101 30.356068283541262 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.11570082139751747 2306 2 0.1344249672385871 2306 3 0.0864401459372317 2306 4 0.11772145074199614 2306 5 0.16185938182184856 2306 6 0.17301912803609554 2306 7 0.1581036992348217 2306 8 0.17803961580101202 2306 9 0.22345913914285376 2306 10-11 0.19633400492712383 2306 12-13 0.20602121501442383 2306 14-15 0.3747688842876542 2306 16-17 0.4042515938053981 2306 18-19 0.3858023216581472 2306 20-21 0.4061128745533651 2306 22-23 0.39165495671572614 2306 24-25 0.4151204619596598 2306 26-27 0.41646544743485236 2306 28-29 0.49972947759485464 2306 30-31 0.35942310771526564 2306 32-33 0.4070232854929188 2306 34-35 0.34490937269750077 2306 36-37 0.4175050063478949 2306 38-39 0.39931975680516274 2306 40-41 0.35885700315960634 2306 42-43 0.3861843235393181 2306 44-45 0.4592362980523532 2306 46-47 0.40689871530618404 2306 48-49 0.5086372021156436 2306 50-51 0.3624231980718662 2306 52-53 0.29040596268732344 2306 54-55 0.27122929880613356 2306 56-57 0.3024681583285158 2306 58-59 0.2567963118474381 2306 60-61 0.3132621873614596 2306 62-63 0.3332215771336777 2306 64-65 0.3197398654790078 2306 66-67 0.2834153674716262 2306 68-69 0.2942673719666331 2306 70-71 0.5119370321478698 2306 72-73 0.4863719268744475 2306 74-75 0.2977973849158957 2306 76-77 -0.08875725558483438 2306 78-79 0.49190847868468524 2306 80-81 0.49112971106466574 2306 82-83 0.43697389591863356 2306 84-85 0.5005675492309436 2306 86-87 0.3994420277828965 2306 88-89 0.5988533243600926 2306 90-91 0.5010942177703299 2306 92-93 0.47111851203309385 2306 94-95 0.4114344645739507 2306 96-97 0.4192742708672412 2306 98-99 0.41068716516581816 2306 100-101 0.419258481152454 2307 1 0.14650743653842113 2307 2 0.15653498376579478 2307 3 0.14761801391597373 2307 4 0.08974587362075681 2307 5 0.17298359849504408 2307 6 0.19191124927976233 2307 7 0.18194806667587216 2307 8 0.12859760120927888 2307 9 0.1643243980592004 2307 10-11 0.1779961424850427 2307 12-13 0.18233064403913346 2307 14-15 0.27534834204864467 2307 16-17 0.3361392797956029 2307 18-19 0.280959270951449 2307 20-21 0.2769409975384818 2307 22-23 0.34847719047925096 2307 24-25 0.3847687294424702 2307 26-27 0.31951688762044483 2307 28-29 0.3705167853640603 2307 30-31 0.36295510270387155 2307 32-33 0.3733738018349726 2307 34-35 0.39867365950188827 2307 36-37 0.3595414845034668 2307 38-39 0.3872845918347849 2307 40-41 0.388792302169513 2307 42-43 0.28951141431354444 2307 44-45 0.39265366735197205 2307 46-47 0.44588020412695784 2307 48-49 0.568494786588353 2307 50-51 0.43659036063354506 2307 52-53 0.2691063352296865 2307 54-55 0.4941939504630568 2307 56-57 0.5024112536361827 2307 58-59 0.5949781773512441 2307 60-61 0.5252788967559354 2307 62-63 0.3218826369332959 2307 64-65 0.3725813734888419 2307 66-67 0.33393040817445296 2307 68-69 0.3399940188605868 2307 70-71 0.2930692817287692 2307 72-73 0.27790554973947934 2307 74-75 0.3175093596270244 2307 76-77 0.11228252682865758 2307 78-79 0.44710815973446927 2307 80-81 0.6848546969567977 2307 82-83 0.3971151757181772 2307 84-85 0.5992835853518201 2307 86-87 0.41617744935675205 2307 88-89 0.610839392766394 2307 90-91 0.5999072639696053 2307 92-93 0.506262187508348 2307 94-95 0.47303527283352764 2307 96-97 0.3540834981819643 2307 98-99 0.28578964684924557 2307 100-101 0.26778138440382904 2201 1 0.1396951324082849 2201 2 0.13382753255779534 2201 3 0.12209761835018895 2201 4 0.10117472797945481 2201 5 0.13392472626189544 2201 6 0.09099777302070322 2201 7 0.06414884406078158 2201 8 0.1326144967550391 2201 9 0.1253178752672497 2201 10-11 0.17519235283249657 2201 12-13 0.17189522562784276 2201 14-15 0.29416667676563435 2201 16-17 0.3241682777757262 2201 18-19 0.35006268232986315 2201 20-21 0.33806950020176174 2201 22-23 0.34192345304720106 2201 24-25 0.2926129966457083 2201 26-27 0.28946233417321565 2201 28-29 0.3811083065425436 2201 30-31 0.29453984149218826 2201 32-33 0.2612592213723346 2201 34-35 0.27956458338098145 2201 36-37 0.28211292171998537 2201 38-39 0.30316905412522743 2201 40-41 0.24937731620071446 2201 42-43 0.3451770078023344 2201 44-45 0.2591366324655837 2201 46-47 0.3759691586355487 2201 48-49 0.5058036659991387 2201 50-51 0.3666562349697884 2201 52-53 0.21957864925206394 2201 54-55 0.287911278265625 2201 56-57 0.3414124383324122 2201 58-59 0.40224799324797544 2201 60-61 0.33160448861837466 2201 62-63 0.3623218066247418 2201 64-65 0.22665339497325476 2201 66-67 0.35858080588407404 2201 68-69 0.2515732768601282 2201 70-71 0.4024300580890383 2201 72-73 0.14410162444256258 2201 74-75 0.2604544429142166 2201 76-77 0.08130175789874272 2201 78-79 0.40366714930257075 2201 80-81 0.4875723464204711 2201 82-83 0.28934697423007805 2201 84-85 0.3484632292071339 2201 86-87 0.19734341840336356 2201 88-89 0.45874415151111236 2201 90-91 0.46574725072493806 2201 92-93 0.32238159477857664 2201 94-95 0.16134334748985424 2201 96-97 0.2818429885167504 2201 98-99 0.24022233793201053 2201 100-101 0.3583800006937743 1101 1 0.0668494317031616 1101 2 0.11597413550319402 1101 3 0.08918758554558792 1101 4 0.0972903895760453 1101 5 0.15304377312262574 1101 6 0.1498020092998118 1101 7 0.1371290798729703 1101 8 0.18147968724341013 1101 9 0.14025503756648305 1101 10-11 0.10212241387456089 1101 12-13 0.15002624980007084 1101 14-15 0.2425697175885304 1101 16-17 0.1622437056091286 1101 18-19 0.15065011830409958 1101 20-21 0.21062443249413576 1101 22-23 0.17887380653328933 1101 24-25 0.23207510096932538 1101 26-27 0.2686878779264603 1101 28-29 0.21067303565787654 1101 30-31 0.23686173858310866 1101 32-33 0.17454514761948303 1101 34-35 0.18918821298598942 1101 36-37 0.239085495855889 1101 38-39 0.15965253235116705 1101 40-41 0.209208663304274 1101 42-43 0.2385464430890707 1101 44-45 0.21450306138296327 1101 46-47 0.27278351634769393 1101 48-49 0.3316685227776972 1101 50-51 0.26203451866580707 1101 52-53 0.2977584904488637 1101 54-55 0.3746166837214986 1101 56-57 0.2857518129065397 1101 58-59 0.38154158901603097 1101 60-61 0.32328147225811676 1101 62-63 0.3005139073822036 1101 64-65 0.24079009299693155 1101 66-67 0.18222779454907823 1101 68-69 0.49807599164523 1101 70-71 0.4166067244142013 1101 72-73 0.2955620716702114 1101 74-75 0.40199898595847117 1101 76-77 0.026851634652036438 1101 78-79 0.3900543929582838 1101 80-81 0.4575926686740175 1101 82-83 0.3666248418635405 1101 84-85 0.3478319732265973 1101 86-87 0.3509351684438826 1101 88-89 0.4002574651523787 1101 90-91 0.5742351843770805 1101 92-93 0.39415544683325976 1101 94-95 0.32800143738001 1101 96-97 0.3930493942391706 1101 98-99 0.21839454632069533 1101 100-101 0.4057501256788463 2304 1 0.15389554592764654 2304 2 0.15454873580956274 2304 3 0.10790832579050402 2304 4 0.13570140039896472 2304 5 0.18624493415771326 2304 6 0.12166261705225168 2304 7 0.15936124086503867 2304 8 0.11232686064726494 2304 9 0.13689870165073614 2304 10-11 0.09956276374593642 2304 12-13 0.12542479280309493 2304 14-15 0.21076932277257043 2304 16-17 0.23820025384101484 2304 18-19 0.18495813478911316 2304 20-21 0.20866436033465874 2304 22-23 0.24503449643066233 2304 24-25 0.2241137461082161 2304 26-27 0.25646344115954633 2304 28-29 0.23627554022787223 2304 30-31 0.22168868892494942 2304 32-33 0.29279382964855216 2304 34-35 0.3489115816686521 2304 36-37 0.3626318256983154 2304 38-39 0.3434858391699933 2304 40-41 0.2712763643367353 2304 42-43 0.3429247880437529 2304 44-45 0.3811322075259014 2304 46-47 0.26951798009935857 2304 48-49 0.33277062562795123 2304 50-51 0.32226046225695626 2304 52-53 0.2628258823122209 2304 54-55 0.484332448244686 2304 56-57 0.4143143866343735 2304 58-59 0.3760068114927222 2304 60-61 0.315503217552795 2304 62-63 0.30583281682566366 2304 64-65 0.286824023556953 2304 66-67 0.30661008166012493 2304 68-69 0.13206053118836536 2304 70-71 0.43071494160900414 2304 72-73 0.12851394634565594 2304 74-75 0.12132996000126894 2304 76-77 0.07235802664845892 2304 78-79 0.21297778205211415 2304 80-81 0.39446188613597144 2304 82-83 0.269496962913756 2304 84-85 0.3255427810384717 2304 86-87 0.2243003992251147 2304 88-89 0.3344867385649337 2304 90-91 0.4364363083919187 2304 92-93 0.13687046194652908 2304 94-95 0.10129843083542767 2304 96-97 0.2917299725154905 2304 98-99 0.2198128221175537 2304 100-101 0.19503136251539743 2202 1 0.1296876593504237 2202 2 0.14042097429503997 2202 3 0.08602486662481112 2202 4 0.056664680247259014 2202 5 0.13193455232119788 2202 6 0.13065981420275818 2202 7 0.09275653314534793 2202 8 0.09245087909880567 2202 9 0.11764366323193798 2202 10-11 0.09047032695315238 2202 12-13 0.048329607693979426 2202 14-15 0.14385133947828166 2202 16-17 0.21627096361904563 2202 18-19 0.13181741178776463 2202 20-21 0.20177732557306172 2202 22-23 0.15499271475125198 2202 24-25 0.17079656387117836 2202 26-27 0.14300402020884206 2202 28-29 0.22630718879610612 2202 30-31 0.18259528136464098 2202 32-33 0.1711483438915664 2202 34-35 0.31519094690272453 2202 36-37 0.2553429666672997 2202 38-39 0.2161593329236311 2202 40-41 0.35356795969453714 2202 42-43 0.3035293209479377 2202 44-45 0.15967654356152394 2202 46-47 0.33770115194106864 2202 48-49 0.34978709734826197 2202 50-51 0.2881685379211305 2202 52-53 0.34183591602607777 2202 54-55 0.4483578730715081 2202 56-57 0.484620870703921 2202 58-59 0.45606158057187685 2202 60-61 0.3990062418223701 2202 62-63 0.3139613510866113 2202 64-65 0.25825957600295624 2202 66-67 0.3225851054748716 2202 68-69 0.24152607532288783 2202 70-71 0.3107478007359461 2202 72-73 0.25100537284405533 2202 74-75 0.3642898649820836 2202 76-77 0.06413144873546983 2202 78-79 0.4629938325908185 2202 80-81 0.540479148006888 2202 82-83 0.4265206399722743 2202 84-85 0.31026827621693087 2202 86-87 0.41774439232067806 2202 88-89 0.5389010814810149 2202 90-91 0.5896887856862136 2202 92-93 0.4387666880587844 2202 94-95 0.42369303197567376 2202 96-97 0.34686241266535234 2202 98-99 0.21178155798873632 2202 100-101 0.24096316977124488 1102 1 0.07945718434503135 1102 2 0.08722009161149913 1102 3 0.09993817559099938 1102 4 0.08597196084669889 1102 5 0.13836539321874142 1102 6 0.07758686625914635 1102 7 0.052540455158926136 1102 8 0.04766421674354859 1102 9 0.06854571442219282 1102 10-11 0.0614425239132288 1102 12-13 0.08896888682291859 1102 14-15 0.19619000095971018 1102 16-17 0.13089374199348924 1102 18-19 0.1422651448828134 1102 20-21 0.18049390495298923 1102 22-23 0.14861140570999964 1102 24-25 0.12335623306694288 1102 26-27 0.21956863140271565 1102 28-29 0.23977801919421893 1102 30-31 0.20889359943482333 1102 32-33 0.18492902657587962 1102 34-35 0.2424833245184388 1102 36-37 0.26194285344475077 1102 38-39 0.1573479675550047 1102 40-41 0.2616358119018187 1102 42-43 0.2967479075860453 1102 44-45 0.17962146125022826 1102 46-47 0.22482325425229277 1102 48-49 0.19452794499199655 1102 50-51 0.18931829049642346 1102 52-53 0.10659728134658764 1102 54-55 0.33782520502288094 1102 56-57 0.26558637078861125 1102 58-59 0.20227571674112 1102 60-61 0.11156773711061874 1102 62-63 0.21867078103779392 1102 64-65 0.23543663792361258 1102 66-67 0.1622981238541712 1102 68-69 0.11835039343734621 1102 70-71 0.09098514945176106 1102 72-73 0.10949703970319291 1102 74-75 0.12037837368765736 1102 76-77 -0.11959246147948477 1102 78-79 0.289128385773175 1102 80-81 0.46249618744663934 1102 82-83 0.3143023249624193 1102 84-85 0.22176314944425002 1102 86-87 0.21483333866291332 1102 88-89 0.35682630840729246 1102 90-91 0.36867821431895464 1102 92-93 0.3525972574724321 1102 94-95 0.309875713160622 1102 96-97 0.12263160901143522 1102 98-99 0.10671534830230911 1102 100-101 0.1110493509908359 2305 1 0.08601241491805922 2305 2 0.10348159903826826 2305 3 0.09716686516547668 2305 4 0.04025191383881577 2305 5 0.11096217972136913 2305 6 0.05402703640665152 2305 7 0.05239055639106027 2305 8 0.04185974887206356 2305 9 -0.011332045699177229 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-1.2508515828575462 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 7.0 9 42.0 10 53.0 11 63.0 12 75.0 13 167.0 14 453.0 15 1121.0 16 2109.0 17 3563.0 18 5438.0 19 7417.0 20 9897.0 21 12821.0 22 16217.0 23 20783.0 24 26108.0 25 32789.0 26 41809.0 27 55364.0 28 76535.0 29 106207.0 30 112510.0 31 114625.0 32 148222.0 33 208317.0 34 316657.0 35 544501.0 36 1055596.0 37 1537321.0 38 359359.0 39 181.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.029254998848714 24.960183307586213 23.92706052033246 15.083501173232616 2 28.871677993758315 23.741193759811672 33.624384879023005 13.762743367407001 3 37.05106108822716 19.109242744837406 27.42561814195002 16.41407802498542 4 27.957776514369698 19.19774878953001 34.5114659820453 18.33300871405499 5 33.528295097780905 18.138544937440944 32.06305051004614 16.27010945473202 6 26.86801503800924 19.415118859197534 37.43558631480532 16.281279787987906 7 31.97532809739534 21.46385763929333 29.16486809767356 17.39594616563777 8 27.81078701226598 28.591007798678202 26.457993214333985 17.14021197472183 9 31.75050956859467 23.856634378589998 25.73599104214019 18.65686501067514 10-11 24.950150278470144 28.817286482952266 28.900847769690184 17.33171546888741 12-13 23.99266121562709 27.03972257709139 28.884696207422706 20.082919999858813 14-15 22.97258970473155 27.527646055740796 29.59679457113499 19.90296966839267 16-17 23.141084861534573 27.152121940643152 30.45154584614094 19.255247351681334 18-19 21.182224177607488 27.42061717758662 31.654018200793406 19.74314044401249 20-21 22.469052674765365 27.565766375179102 31.20112849433666 18.76405245571887 22-23 21.34141999020416 29.88663149880334 29.851106517017424 18.92084199397508 24-25 21.38527081517895 27.175687126024823 31.119022392365636 20.320019666430593 26-27 21.318300722396664 27.38514125592334 29.609397530774995 21.687160490905004 28-29 21.971048904674078 28.885723961133053 29.38232624889205 19.760900885300817 30-31 20.456025740766464 28.2184937947553 30.501809573224755 20.823670891253485 32-33 20.393737637109094 29.226211498425457 29.812602087606553 20.56744877685889 34-35 20.63349492943692 29.377779217325063 31.064478776262998 18.924247076975014 36-37 20.469812174375964 28.52340444350874 31.052934714385167 19.95384866773013 38-39 22.187975607375922 29.387723957219475 29.280160125908132 19.14414030949647 40-41 20.864176061934796 30.386163299354813 29.064764409293335 19.684896229417056 42-43 20.923473853988966 29.39283465914093 30.52751929743548 19.156172189434624 44-45 20.530144326883057 28.30812823533308 30.77546047452434 20.38626696325952 46-47 21.863086862581774 28.141174990222655 29.492997324220894 20.502740822974676 48-49 20.841591133041238 29.637960826233677 30.091780323995415 19.428667716729674 50-51 21.9935231241577 28.445554988817097 29.769303993838115 19.79161789318709 52-53 20.604379403812047 28.1851342130956 28.915616244026683 22.294870139065672 54-55 22.072920557164448 28.420260876598785 29.78172094491891 19.725097621317854 56-57 20.866741324876912 28.403845884271465 29.60504353072071 21.12436926013091 58-59 20.811192334579584 28.35719362139858 29.923557219623614 20.908056824398223 60-61 21.726893964449385 28.69146370095524 29.88966846542585 19.691973869169527 62-63 20.593292171653093 28.66258524533588 29.804377501917646 20.939745081093385 64-65 21.924002050855655 28.502044729668523 29.64957696429754 19.924376255178274 66-67 21.078268642067506 30.391532714742496 28.42695872012567 20.103239923064326 68-69 21.201233264240603 30.413529395944135 28.34083052294728 20.044406816867983 70-71 20.97235026774119 31.05033509445969 28.224509238073143 19.752805399725975 72-73 20.653742640429297 31.47804467209187 28.07568298467149 19.792529702807347 74-75 21.10196686408451 30.788470543250934 28.03462820620529 20.074934386459265 76-77 21.205879561371464 30.13213934944258 28.37044750719483 20.291533581991132 78-79 20.98340450386138 29.914465572068274 28.82579271854111 20.276337205529234 80-81 20.58874250149168 30.119161120166233 28.721201102728074 20.570895275614014 82-83 19.880352661180016 30.068710274358356 29.019914404138085 21.031022660323544 84-85 20.01676306171207 29.846586734984154 29.084244300081437 21.052405903222336 86-87 19.999194185090076 29.97834995482348 29.48678209134514 20.535673768741297 88-89 20.172810712976588 30.20260244086565 29.218852379859587 20.405734466298174 90-91 20.325887637556043 29.77661874313388 28.95688958758626 20.940604031723815 92-93 19.973596862033478 30.55249776157017 28.799776826910307 20.674128549486046 94-95 19.708196890553477 30.89157318243884 28.775492759708825 20.62473716729885 96-97 20.083758483046577 31.033891892840572 28.50046103824421 20.381888585868644 98-99 20.363149068567463 30.428430198460575 28.215641153917215 20.992779579054748 100-101 20.393817507355376 30.44972033010156 27.75191347758928 21.404548684953784 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 49.0 1 43.0 2 66.5 3 177.0 4 233.5 5 161.5 6 108.5 7 99.5 8 101.0 9 109.5 10 103.5 11 103.0 12 116.0 13 136.0 14 141.5 15 148.5 16 182.0 17 248.5 18 316.0 19 420.5 20 609.5 21 913.5 22 1427.5 23 2187.5 24 3182.5 25 4798.0 26 7620.5 27 11596.0 28 15861.0 29 20319.0 30 25825.5 31 32421.0 32 45296.5 33 68762.5 34 102275.5 35 142187.0 36 197431.5 37 266190.5 38 326254.5 39 349846.5 40 345676.5 41 323518.0 42 297199.5 43 294967.0 44 286286.5 45 263213.0 46 246289.0 47 217583.5 48 174731.5 49 143576.0 50 120761.5 51 101755.0 52 86357.0 53 70346.0 54 54385.5 55 40425.0 56 30289.5 57 22701.0 58 17543.5 59 13628.0 60 10184.5 61 7475.0 62 5485.5 63 4020.5 64 2933.0 65 2079.0 66 1447.5 67 1003.5 68 697.0 69 496.5 70 357.0 71 265.5 72 190.0 73 113.0 74 76.5 75 63.5 76 51.5 77 34.5 78 18.0 79 13.0 80 11.5 81 10.0 82 9.5 83 5.0 84 1.5 85 1.5 86 2.5 87 2.0 88 0.5 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18005414497223923 2 0.0 3 0.0 4 0.008948724225442241 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.002148939576179285 12-13 0.0 14-15 0.0 16-17 3.8410997255378527E-4 18-19 1.0381350609561763E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0024707614450757 40-41 0.006156140911470125 42-43 0.0030105916767729115 44-45 0.001546821240824703 46-47 0.007235801374864549 48-49 0.008876054771175307 50-51 0.005657836082211161 52-53 0.006872454103529888 54-55 0.01890443946001197 56-57 0.021831980331908387 58-59 0.028133460151912378 60-61 0.025963757874513972 62-63 0.010858892737601605 64-65 0.016153381548478105 66-67 0.02361757263675301 68-69 0.027043418337908395 70-71 0.029441510328717165 72-73 0.022537912173358588 74-75 0.03287773738048211 76-77 0.02931693412140242 78-79 0.021105285789239064 80-81 0.009436647704091644 82-83 0.0018997871615498026 84-85 0.007952114566924311 86-87 0.027562485868386482 88-89 0.025621173304398434 90-91 0.021157192542286873 92-93 0.04419340954490443 94-95 0.04552222242292833 96-97 0.03431036376460163 98-99 0.04147349568519925 100-101 0.041369682179103626 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4816329.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.26683138215342 #Duplication Level Percentage of deduplicated Percentage of total 1 91.00054923221322 43.01307616242372 2 4.379269443022183 4.139883806806929 3 1.3207474434843771 1.8728264012875873 4 0.7236960977188277 1.36827285691193 5 0.42437131982016785 1.0029343808680888 6 0.2740531328549564 0.7772173932243668 7 0.21553595511016196 0.7131391152888389 8 0.1430125076412761 0.5407798467375299 9 0.14251466543071534 0.6062594994357867 >10 1.073863737923304 9.976643891976217 >50 0.12549965397773447 4.25190898562576 >100 0.13894354305119688 14.118373799355794 >500 0.029142420097735502 9.404671040215579 >1k 0.00862466528624524 5.67593627744888 >5k 1.3213686203462007E-4 0.4552482240435293 >10k+ 4.4045505194904095E-5 2.0828283183495295 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 100045 2.0772044434672132 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8285 0.1720189796004384 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7487 0.15545034402757785 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6095 0.12654866393055791 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0012872874755856588 0.0 0.0 2 0.0013495755792430292 0.0 0.0 3 0.001536439890215141 0.0 0.0 4 0.0016402533963107587 0.0 0.0 5 0.0018686431097211175 0.0 0.0 6 0.0019932193170358586 0.0 0.0 7 0.002283897134103588 0.0 0.0 8 0.002595337652390441 0.0 0.0 9 0.0031974559877450234 0.0 0.0 10-11 0.0049415228901514 0.0 0.0 12-13 0.007111225167549808 0.0 1.0381350609561763E-5 14-15 0.009229020691900408 0.0 2.0762701219123527E-5 16-17 0.01159596863088049 0.0 2.0762701219123527E-5 18-19 0.014803805969235074 0.0 2.0762701219123527E-5 20-21 0.018582617591115555 0.0 3.114405182868529E-5 22-23 0.023098505106274925 0.0 4.1525402438247054E-5 24-25 0.02773896882874903 0.0 4.1525402438247054E-5 26-27 0.03314765249633071 0.0 4.1525402438247054E-5 28-29 0.03919997990170522 0.0 4.1525402438247054E-5 30-31 0.06004573192570524 0.0 6.228810365737058E-5 32-33 0.06741649085849409 0.0 6.228810365737058E-5 34-35 0.07414360605349013 0.0 6.228810365737058E-5 36-37 0.08150398363566941 0.0 6.228810365737058E-5 38-39 0.0904942332635499 0.0 6.228810365737058E-5 40-41 0.09951562694325908 0.0 6.228810365737058E-5 42-43 0.10904570680283676 0.0 8.305080487649411E-5 44-45 0.12017451465628698 0.0 8.305080487649411E-5 46-47 0.1319573475981396 0.0 8.305080487649411E-5 48-49 0.14456030723814756 0.0 8.305080487649411E-5 50-51 0.15754737685070933 0.0 9.343215548605587E-5 52-53 0.17136495451203604 0.0 1.0381350609561763E-4 54-55 0.18607532832578505 0.0 1.141948567051794E-4 56-57 0.20212489636816755 0.0 1.2457620731474116E-4 58-59 0.21944098918491656 0.0 1.453389085338647E-4 60-61 0.23741110709006796 0.0 1.453389085338647E-4 62-63 0.25824647776345844 0.0 1.453389085338647E-4 64-65 0.281376126921562 0.0 1.453389085338647E-4 66-67 0.3060318346192712 0.0 1.453389085338647E-4 68-69 0.3326184735303589 0.0 1.453389085338647E-4 70-71 0.36238180572797246 0.0 1.453389085338647E-4 72-73 0.39407606913896454 0.0 1.5572025914342645E-4 74-75 0.428863975031606 0.0 1.6610160975298821E-4 76-77 0.46610187966810407 0.0 1.7648296036254998E-4 78-79 0.5096827895270444 0.0 1.8686431097211174E-4 80-81 0.5553192068066779 0.0 2.0762701219123527E-4 82-83 0.605772570769148 0.0 2.0762701219123527E-4 84-85 0.6611466949205504 0.0 2.1800836280079703E-4 86-87 0.7244106455352199 0.0 2.283897134103588E-4 88-89 0.7911108232016542 0.0 2.283897134103588E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 17155 0.0 66.24658 3 CGGAAGA 17085 0.0 66.212234 4 GATCGGA 17335 0.0 65.294464 1 ATCGGAA 17540 0.0 64.38634 2 GAAGAGC 18110 0.0 62.544693 6 GGAAGAG 20535 0.0 55.181847 5 TTGTGTT 57555 0.0 46.15198 1 GCCGTCT 12945 0.0 43.704147 50-51 ATGCCGT 13050 0.0 43.241512 48-49 TGCCGTC 12990 0.0 43.07736 50-51 TATGCCG 12945 0.0 43.060192 48-49 TCTCGTA 12890 0.0 43.018353 42-43 CGTCTTC 13180 0.0 42.94582 52-53 TCGTATG 13245 0.0 42.7273 44-45 CTCGTAT 12930 0.0 42.574375 44-45 AATCTCG 12915 0.0 42.547176 40-41 CGTATGC 13190 0.0 42.384205 46-47 ATCTCGT 12980 0.0 42.171215 42-43 GTATGCC 13500 0.0 41.85073 46-47 CCGTCTT 13605 0.0 41.09795 52-53 >>END_MODULE