FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_34344-1.34100000035857.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_34344-1.34100000035857.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4816329
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1000452.0772044434672132TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC82850.1720189796004384No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA74870.15545034402757785No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG60950.12654866393055791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG171550.066.246583
CGGAAGA170850.066.2122344
GATCGGA173350.065.2944641
ATCGGAA175400.064.386342
GAAGAGC181100.062.5446936
GGAAGAG205350.055.1818475
TTGTGTT575550.046.151981
GCCGTCT129450.043.70414750-51
ATGCCGT130500.043.24151248-49
TGCCGTC129900.043.0773650-51
TATGCCG129450.043.06019248-49
TCTCGTA128900.043.01835342-43
CGTCTTC131800.042.9458252-53
TCGTATG132450.042.727344-45
CTCGTAT129300.042.57437544-45
AATCTCG129150.042.54717640-41
CGTATGC131900.042.38420546-47
ATCTCGT129800.042.17121542-43
GTATGCC135000.041.8507346-47
CCGTCTT136050.041.0979552-53
Produced by FastQC (version 0.11.2)