##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_34311-1.34100000035764.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2664865 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65750947984232 33.0 33.0 33.0 33.0 33.0 2 32.67669619286531 33.0 33.0 33.0 33.0 33.0 3 32.728252275443594 33.0 33.0 33.0 33.0 33.0 4 36.68063898171202 37.0 37.0 37.0 37.0 37.0 5 36.63644950119424 37.0 37.0 37.0 37.0 37.0 6 36.54918691941243 37.0 37.0 37.0 37.0 37.0 7 36.51615935516433 37.0 37.0 37.0 37.0 37.0 8 36.447765646665026 37.0 37.0 37.0 37.0 37.0 9 36.451135798623945 37.0 37.0 37.0 37.0 37.0 10-11 36.419866672420554 37.0 37.0 37.0 37.0 37.0 12-13 36.406085674133585 37.0 37.0 37.0 37.0 37.0 14-15 38.26012518457783 40.0 37.0 40.0 37.0 40.0 16-17 38.25882249194612 40.0 37.0 40.0 37.0 40.0 18-19 38.27286823159898 40.0 37.0 40.0 37.0 40.0 20-21 38.214550455651604 40.0 37.0 40.0 37.0 40.0 22-23 38.12701318828533 40.0 37.0 40.0 37.0 40.0 24-25 38.09429914836211 40.0 37.0 40.0 37.0 40.0 26-27 38.00909333118188 40.0 37.0 40.0 37.0 40.0 28-29 37.90502689629681 40.0 37.0 40.0 33.0 40.0 30-31 37.86955211614847 40.0 37.0 40.0 33.0 40.0 32-33 37.75781925163189 40.0 37.0 40.0 33.0 40.0 34-35 37.69172490914174 40.0 37.0 40.0 33.0 40.0 36-37 37.62730325926454 40.0 37.0 40.0 33.0 40.0 38-39 37.532701281303176 40.0 37.0 40.0 33.0 40.0 40-41 37.4439652665332 40.0 37.0 40.0 33.0 40.0 42-43 37.33095222459674 40.0 37.0 40.0 33.0 40.0 44-45 37.213757357314535 40.0 37.0 40.0 33.0 40.0 46-47 37.17814429624015 40.0 37.0 40.0 33.0 40.0 48-49 36.973291329954804 38.5 37.0 40.0 33.0 40.0 50-51 36.81102157144921 37.0 37.0 40.0 33.0 40.0 52-53 36.701217885333776 37.0 37.0 40.0 33.0 40.0 54-55 36.47308268899175 37.0 37.0 40.0 33.0 40.0 56-57 36.34354629596621 37.0 37.0 40.0 33.0 40.0 58-59 36.10148863075615 37.0 37.0 40.0 33.0 40.0 60-61 35.84340557589221 37.0 37.0 40.0 33.0 40.0 62-63 35.5547823248082 37.0 35.0 40.0 27.0 40.0 64-65 35.30092462469956 37.0 33.0 37.0 27.0 40.0 66-67 34.99483275888272 37.0 33.0 37.0 27.0 40.0 68-69 34.735421118893456 37.0 33.0 37.0 27.0 40.0 70-71 34.31847110454 37.0 33.0 37.0 27.0 40.0 72-73 34.074166796441844 37.0 33.0 37.0 27.0 37.0 74-75 33.71536344242578 37.0 33.0 37.0 27.0 37.0 76-77 31.10763434545465 33.0 30.0 35.0 24.5 37.0 78-79 33.39051959480123 35.0 33.0 37.0 27.0 37.0 80-81 33.78429001093864 37.0 33.0 37.0 27.0 37.0 82-83 33.7984618357778 37.0 33.0 37.0 27.0 37.0 84-85 33.68348565499565 37.0 33.0 37.0 27.0 37.0 86-87 33.575549980955884 37.0 33.0 37.0 27.0 37.0 88-89 33.38545048248223 37.0 33.0 37.0 27.0 37.0 90-91 33.157935767853154 37.0 33.0 37.0 27.0 37.0 92-93 33.16543277051558 37.0 33.0 37.0 27.0 37.0 94-95 33.11672035919268 37.0 33.0 37.0 27.0 37.0 96-97 32.96569507273351 33.0 33.0 37.0 27.0 37.0 98-99 32.81705977601116 33.0 33.0 37.0 27.0 37.0 100-101 31.276332196940558 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.14938971243378774 2306 2 0.16028347284338196 2306 3 0.13394943503944745 2306 4 0.1668962262771032 2306 5 0.1831260530390253 2306 6 0.18439089507814543 2306 7 0.16991871681766213 2306 8 0.19713607166477942 2306 9 0.20718505492070705 2306 10-11 0.2155153793039588 2306 12-13 0.23970683236976242 2306 14-15 0.39249397771204997 2306 16-17 0.4098862054539367 2306 18-19 0.41933291415149654 2306 20-21 0.3978476806487876 2306 22-23 0.39572956262959025 2306 24-25 0.4901951454559992 2306 26-27 0.4012189898122429 2306 28-29 0.4924285142650575 2306 30-31 0.48579613721224035 2306 32-33 0.5488130680165497 2306 34-35 0.5133563632682794 2306 36-37 0.5851007404265332 2306 38-39 0.6515857574010226 2306 40-41 0.655763575332557 2306 42-43 0.39950759422568893 2306 44-45 0.549762339385282 2306 46-47 0.5935016575555778 2306 48-49 0.5948678436304249 2306 50-51 0.37807176916630425 2306 52-53 0.3557145993038233 2306 54-55 0.38100725077573827 2306 56-57 0.6072084284560049 2306 58-59 0.6231223061139062 2306 60-61 0.6281927247807886 2306 62-63 0.5574549136345937 2306 64-65 0.46915803511470955 2306 66-67 0.5145756676253157 2306 68-69 0.6254950242830688 2306 70-71 0.6536926091351987 2306 72-73 0.5891752186963615 2306 74-75 0.5494493442061383 2306 76-77 -0.11745265166880259 2306 78-79 0.3827200613863795 2306 80-81 0.4633464471287638 2306 82-83 0.542505831689887 2306 84-85 0.5334289568950297 2306 86-87 0.4593674600019355 2306 88-89 0.5603460398100495 2306 90-91 0.46405456680519563 2306 92-93 0.4947768567788131 2306 94-95 0.5393191021773021 2306 96-97 0.3725213733156423 2306 98-99 0.3271970690878945 2306 100-101 0.4746512411726336 2307 1 0.1887357160556249 2307 2 0.17638553345570074 2307 3 0.12328037840180883 2307 4 0.12313369398975027 2307 5 0.20171668322175407 2307 6 0.1658978799650228 2307 7 0.1882167476733656 2307 8 0.27606535483331385 2307 9 0.1903458407097247 2307 10-11 0.19599900419162708 2307 12-13 0.19281668374652128 2307 14-15 0.3070376395557446 2307 16-17 0.3808013405844193 2307 18-19 0.35213120871542003 2307 20-21 0.3520031443265097 2307 22-23 0.35010595109330467 2307 24-25 0.3754326488215014 2307 26-27 0.3930600174014103 2307 28-29 0.4035911785909292 2307 30-31 0.41469782015013124 2307 32-33 0.3816928723929962 2307 34-35 0.41923933091393195 2307 36-37 0.38563885271828724 2307 38-39 0.45551507130478086 2307 40-41 0.4206552213758812 2307 42-43 0.38908712407511814 2307 44-45 0.31418324474661574 2307 46-47 0.30670280484000045 2307 48-49 0.3429158495906037 2307 50-51 0.3072634696724492 2307 52-53 0.24306250605069835 2307 54-55 0.411769521198984 2307 56-57 0.4727429324325314 2307 58-59 0.4516599610655305 2307 60-61 0.37837461583303167 2307 62-63 0.2220828514609252 2307 64-65 0.3436280061867194 2307 66-67 0.17802570874903978 2307 68-69 0.34793296679345076 2307 70-71 0.3687360257118044 2307 72-73 0.2625641704900019 2307 74-75 0.2969414114449762 2307 76-77 -0.005643072360246748 2307 78-79 0.3401659151432668 2307 80-81 0.4592687435663052 2307 82-83 0.3327960828458387 2307 84-85 0.28262867314744966 2307 86-87 0.15479934521447802 2307 88-89 0.34488548478109493 2307 90-91 0.40274357429106544 2307 92-93 0.2731042213317565 2307 94-95 0.42352857378963193 2307 96-97 0.3143784446914353 2307 98-99 0.22726726833820265 2307 100-101 0.1376396392404935 2201 1 0.14689253918319878 2201 2 0.14937982570283737 2201 3 0.14707848185447858 2201 4 0.1617926126012179 2201 5 0.17241457415471473 2201 6 0.15167589733447784 2201 7 0.19705145488573805 2201 8 0.10655966830412211 2201 9 0.21952768230189434 2201 10-11 0.2588567999293403 2201 12-13 0.21810539643176696 2201 14-15 0.3327154528949663 2201 16-17 0.407063512360331 2201 18-19 0.3901116772774955 2201 20-21 0.3707004919334693 2201 22-23 0.3162831998828466 2201 24-25 0.3993644614079841 2201 26-27 0.3562914205125125 2201 28-29 0.48005723910952014 2201 30-31 0.3941246536631624 2201 32-33 0.4136599672118777 2201 34-35 0.42852079642262453 2201 36-37 0.4380238274904116 2201 38-39 0.22853532717736869 2201 40-41 0.45237046195327935 2201 42-43 0.440172856390852 2201 44-45 0.43932402843777396 2201 46-47 0.4330922479025432 2201 48-49 0.4865367919782315 2201 50-51 0.5492540095743621 2201 52-53 0.4193388976868775 2201 54-55 0.4096436167168278 2201 56-57 0.5713495276178691 2201 58-59 0.5280853428793932 2201 60-61 0.48580707503405307 2201 62-63 0.5032537048506285 2201 64-65 0.42679862470111374 2201 66-67 0.5160844926609371 2201 68-69 0.3875649907677001 2201 70-71 0.5462175681916932 2201 72-73 0.5027758129475401 2201 74-75 0.5138112593985866 2201 76-77 0.2081900218530457 2201 78-79 0.4326717658021906 2201 80-81 0.5314522207961332 2201 82-83 0.4166437364981519 2201 84-85 0.45313647072455154 2201 86-87 0.466970908889742 2201 88-89 0.6772071564140916 2201 90-91 0.7128447687593322 2201 92-93 0.5461046684437818 2201 94-95 0.5224312391998609 2201 96-97 0.5939525704883835 2201 98-99 0.44049407298703613 2201 100-101 0.6026609053598726 1101 1 0.08003210058718935 1101 2 0.11203813207778524 1101 3 0.1185842466606033 1101 4 0.09356509662232781 1101 5 0.17662345737520013 1101 6 0.07106942038913644 1101 7 0.15780625311295893 1101 8 0.11267890190147511 1101 9 0.039773655878114766 1101 10-11 0.16469518282778495 1101 12-13 0.140394094711354 1101 14-15 0.23923007610709135 1101 16-17 0.2270640779846005 1101 18-19 0.23650317685692812 1101 20-21 0.23010180600638108 1101 22-23 0.22347474763157749 1101 24-25 0.3683595842670542 1101 26-27 0.3046075023054371 1101 28-29 0.2458066821520717 1101 30-31 0.36628430750110397 1101 32-33 0.35251370224791856 1101 34-35 0.2715114800434222 1101 36-37 0.20662465549136755 1101 38-39 0.2433134017558416 1101 40-41 0.3649299665394068 1101 42-43 0.3761234336013004 1101 44-45 0.3732315160259745 1101 46-47 0.43621572550656396 1101 48-49 0.4638819432097492 1101 50-51 0.3491505094548515 1101 52-53 0.3296762743214785 1101 54-55 0.44590241354276117 1101 56-57 0.2980394213717332 1101 58-59 0.35582469773928693 1101 60-61 0.39672193580837956 1101 62-63 0.2863000750936848 1101 64-65 0.35977214801122415 1101 66-67 0.372636018295573 1101 68-69 0.31656477546556516 1101 70-71 0.4394655652431467 1101 72-73 0.49057839912769197 1101 74-75 0.5582209757288936 1101 76-77 0.20714931880031529 1101 78-79 0.4192466279996978 1101 80-81 0.459106823244376 1101 82-83 0.48947379194756735 1101 84-85 0.4669114745105887 1101 86-87 0.3166537487615031 1101 88-89 0.4476061222339709 1101 90-91 0.5539568043316265 1101 92-93 0.42144358686068983 1101 94-95 0.3689032490088735 1101 96-97 0.3102940488112864 1101 98-99 0.4063004889909152 1101 100-101 0.42579521193188086 2304 1 0.08255718267221113 2304 2 0.07993107812599476 2304 3 0.07227042977670095 2304 4 0.043736810144622495 2304 5 0.11911908016922723 2304 6 0.09975768788903139 2304 7 0.07997972942840903 2304 8 0.08240809081810596 2304 9 0.0336852700646304 2304 10-11 -0.009986899015054007 2304 12-13 0.07031353824044118 2304 14-15 0.21298947072263275 2304 16-17 0.21624950079689853 2304 18-19 0.17692970133553843 2304 20-21 0.2040063134367145 2304 22-23 0.2483763845676421 2304 24-25 0.200018396446211 2304 26-27 0.20269244987822077 2304 28-29 0.25838722869735165 2304 30-31 0.21559467423354306 2304 32-33 0.2615369004470054 2304 34-35 0.3588322726651043 2304 36-37 0.26947577490472696 2304 38-39 0.22728607846195814 2304 40-41 0.2896134379714326 2304 42-43 0.3534794820860583 2304 44-45 0.31614361855383777 2304 46-47 0.3849543755405094 2304 48-49 0.35457248963592036 2304 50-51 0.3193700248455471 2304 52-53 0.28896647215928084 2304 54-55 0.39952010976268326 2304 56-57 0.3840561837877132 2304 58-59 0.316035796189702 2304 60-61 0.3330980062883384 2304 62-63 0.2398649669240882 2304 64-65 0.18502296886141067 2304 66-67 0.24999663257340643 2304 68-69 0.1673973379077438 2304 70-71 0.2106947164423687 2304 72-73 0.1174578185176216 2304 74-75 0.29052693944870356 2304 76-77 0.1958509660590515 2304 78-79 0.2682921787335957 2304 80-81 0.42984743458421093 2304 82-83 0.34207609548943907 2304 84-85 0.4243940153148813 2304 86-87 0.24523084912557636 2304 88-89 0.5462673522983224 2304 90-91 0.6168619192109404 2304 92-93 0.45063925293049323 2304 94-95 0.5378495036689657 2304 96-97 0.4726328068361667 2304 98-99 0.3617905573984146 2304 100-101 0.4544453649852045 2202 1 0.1101316179674896 2202 2 0.1552193664950039 2202 3 0.06572782940217081 2202 4 0.042955497144539834 2202 5 0.12376395011465746 2202 6 0.05285829985781021 2202 7 0.13662312993196224 2202 8 0.14455652236809868 2202 9 0.18218760414762158 2202 10-11 0.15623968175497538 2202 12-13 0.13624847063715606 2202 14-15 0.2701010318254333 2202 16-17 0.2319201128552848 2202 18-19 0.18152170721330663 2202 20-21 0.2486486745789378 2202 22-23 0.2546864726445577 2202 24-25 0.21997816599471776 2202 26-27 0.22147756192315882 2202 28-29 0.1916234952397744 2202 30-31 0.2825846479807126 2202 32-33 0.3552913244526721 2202 34-35 0.29253266780161624 2202 36-37 0.2929062048956794 2202 38-39 0.41058506508466763 2202 40-41 0.32960689716306035 2202 42-43 0.3814987819507607 2202 44-45 0.30208267255582655 2202 46-47 0.3066358112927432 2202 48-49 0.2824125892712459 2202 50-51 0.3269004664845099 2202 52-53 0.4983462601654125 2202 54-55 0.4369850537178124 2202 56-57 0.5059692358364245 2202 58-59 0.47366569395597224 2202 60-61 0.42180570383418825 2202 62-63 0.30078050089597497 2202 64-65 0.40387796899581474 2202 66-67 0.4022706834841898 2202 68-69 0.3490257303920856 2202 70-71 0.43315925719697645 2202 72-73 0.29843347416645827 2202 74-75 0.35437015716802023 2202 76-77 -0.005014571048832295 2202 78-79 0.45533331414624456 2202 80-81 0.49578012697806173 2202 82-83 0.5215849972608311 2202 84-85 0.49659557973288315 2202 86-87 0.5884109363960874 2202 88-89 0.5775176322986866 2202 90-91 0.6255880743804241 2202 92-93 0.5549310984240563 2202 94-95 0.5137318407983571 2202 96-97 0.30813326827675525 2202 98-99 0.38812895911156176 2202 100-101 0.5752630556318508 1102 1 0.14406121004914496 1102 2 0.11125232045374389 1102 3 0.06924874335476261 1102 4 0.10336861311022005 1102 5 0.13186930470126157 1102 6 0.10858561292905478 1102 7 0.12273701828842576 1102 8 0.09400956332225263 1102 9 0.03611163598262124 1102 10-11 0.16283815769481436 1102 12-13 0.11051488933753717 1102 14-15 0.2806062717535269 1102 16-17 0.10671993534181468 1102 18-19 0.13570562014191268 1102 20-21 0.18521662096572555 1102 22-23 0.18216091191440142 1102 24-25 0.2354177794712342 1102 26-27 0.17144380328276299 1102 28-29 0.2181274815221954 1102 30-31 0.1933468334684676 1102 32-33 0.2740499907532623 1102 34-35 0.2966333497088769 1102 36-37 0.1818044472015572 1102 38-39 0.2101396409777081 1102 40-41 0.2661548991868514 1102 42-43 0.29886885435483634 1102 44-45 0.303014618244994 1102 46-47 0.31658623530471885 1102 48-49 0.31577424187324965 1102 50-51 0.26681302210857183 1102 52-53 0.3020693806034629 1102 54-55 0.30890874364944665 1102 56-57 0.27372593532926004 1102 58-59 0.29915329477861263 1102 60-61 0.2842657503537751 1102 62-63 0.2375939097243318 1102 64-65 0.3236778001162719 1102 66-67 0.25301743151694467 1102 68-69 0.15044117579029148 1102 70-71 0.25298412669523174 1102 72-73 0.21942021073336804 1102 74-75 0.12789155462032653 1102 76-77 -0.07934099551668083 1102 78-79 0.20610976287542826 1102 80-81 0.4004873420024708 1102 82-83 0.2524425530661958 1102 84-85 0.25620798564470704 1102 86-87 0.32595322103582447 1102 88-89 0.45078035740665 1102 90-91 0.3660546688674913 1102 92-93 0.366180064511731 1102 94-95 0.2897771067318331 1102 96-97 0.23180489599579346 1102 98-99 0.21356622416092108 1102 100-101 0.18055426923338658 2305 1 0.046651373322127654 2305 2 0.0896437595770152 2305 3 0.03725988463853014 2305 4 0.07694824660008237 2305 5 0.12008659016093759 2305 6 0.0389917796600443 2305 7 0.015956240111684394 2305 8 0.07156676211354096 2305 9 0.0753879023164501 2305 10-11 0.0957724281553638 2305 12-13 0.049305460784722754 2305 14-15 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-1.4867100682407681 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 6.0 8 52.0 9 162.0 10 118.0 11 55.0 12 43.0 13 128.0 14 335.0 15 890.0 16 1723.0 17 2520.0 18 3166.0 19 3720.0 20 4573.0 21 5597.0 22 6826.0 23 8569.0 24 10934.0 25 13717.0 26 17752.0 27 22784.0 28 30745.0 29 37456.0 30 43299.0 31 54750.0 32 74758.0 33 106116.0 34 165070.0 35 294051.0 36 604774.0 37 941032.0 38 209062.0 39 82.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.9731174185263 24.334627348456994 25.081975594919882 13.610279638096829 2 32.469787400112196 20.967215975293307 33.68249423516764 12.880502389426857 3 38.65944428704643 17.416867270949936 27.938263289134724 15.985425152868906 4 31.70742508358656 17.12791736705243 34.82800252045051 16.3366550289105 5 30.443831113395987 16.103479913616635 37.37667761781553 16.076011355171836 6 25.32604841145799 16.85151030164755 41.72586603824209 16.09657524865237 7 34.37221022453295 17.825030536256058 30.136948776016798 17.665810463194195 8 30.774842252797047 27.718890075106994 24.439924724141747 17.06634294795421 9 29.17982712069842 26.49894084690969 25.41929891382865 18.901933118563228 10-11 24.618907458724472 27.433202911056735 30.179575957795045 17.768313672423748 12-13 24.387389229848417 25.976813084340105 29.62615367007334 20.009644015738136 14-15 23.270803586673246 26.901287682490484 30.740825520242115 19.08708321059416 16-17 22.285717394967158 27.882096487728987 31.553681867357238 18.27850424994662 18-19 20.987704818067705 28.11827240779552 31.888669782521816 19.00535299161496 20-21 21.55711452550129 28.115307904903254 31.48497203423063 18.842605535364832 22-23 20.829985759128512 28.258954956442444 31.847748385002618 19.063310899426426 24-25 21.605297078838888 27.042833314257948 31.892872622065283 19.45899698483788 26-27 21.3875749803461 27.389079747004068 31.66087212673062 19.562473145919213 28-29 21.06354355661544 28.61454520210217 30.974345792375974 19.34756544890642 30-31 20.136892487987197 28.927187681177095 30.905336668086374 20.030583162749334 32-33 19.639174967587476 29.771207922352538 31.38406635983436 19.205550750225623 34-35 20.174380315700795 29.48368866715575 31.62477273708049 18.717158280062968 36-37 20.46501417520212 30.22862321355866 31.33715966850103 17.96920294273819 38-39 21.022255211808407 30.262374784881796 30.318138526741457 18.397231476568347 40-41 21.116559096527165 29.54031708313389 29.913605513275343 19.429518307063603 42-43 20.4580955334813 29.733354573253052 30.89378220113491 18.91476769213074 44-45 19.867117251922778 29.26448792896959 31.820729310926154 19.047665508181478 46-47 20.49181973564886 29.029632313578148 31.224496190999616 19.254051759773375 48-49 20.543575182501474 30.63525374572737 29.76862505798165 19.052546013789502 50-51 20.792905930094687 29.745174371116946 29.700441456078902 19.76147824270946 52-53 19.923308238510025 29.52961185397342 29.8108825294321 20.736197378084455 54-55 21.220941416018054 29.349209700347643 29.92447465833439 19.50537422529991 56-57 21.00829422932129 29.46029707288547 29.331724882913168 20.199683814880068 58-59 20.5573236184445 29.5334860183939 30.276235088948777 19.632955274212822 60-61 20.40366285132166 29.83489317809706 30.38484261795677 19.37660135262451 62-63 20.363952839025796 29.840188457750877 29.879219057741267 19.916639645482057 64-65 20.90659779900722 29.72471827037187 29.54948405552549 19.81919987509542 66-67 21.155919941656183 29.941348401231586 29.101924137276118 19.80080751983611 68-69 21.933412020699443 30.045817967932862 28.617246271249204 19.403523740118487 70-71 21.013214337729103 31.012572464148917 28.758244884905686 19.21596831321629 72-73 20.266710606718025 31.187233413256184 28.8731553811299 19.672900598895893 74-75 21.092057414471864 30.095731452676226 28.742202639864487 20.07000849298742 76-77 21.317828657162487 29.211336170985863 29.156626370349127 20.31420880150252 78-79 21.165095733850045 29.228076924881417 29.644548495610927 19.96227884565761 80-81 19.73531896959529 29.837347282625725 30.022740719210194 20.40459302856879 82-83 19.112464882692763 29.663108388057196 30.120006296864123 21.104420432385922 84-85 19.102671322931855 29.211058773682957 30.59581667935437 21.090453224030817 86-87 19.19376661088182 29.3012154740927 31.620287417404235 19.884730497621245 88-89 19.59186745235551 29.884796665664474 30.940570739208884 19.582765142771134 90-91 19.993776904659402 29.575542109299025 30.022719177380363 20.407961808661213 92-93 19.139788942712542 30.65810018407163 30.18964295810578 20.012467915110054 94-95 18.937672781020993 30.827240847944065 30.407544989470058 19.82754138156488 96-97 19.55042058446375 31.548264357080864 29.414691626450125 19.486623432005263 98-99 19.991031312694467 30.690404968705327 28.905395928688986 20.41316778991122 100-101 19.530616489371287 30.713458731522138 28.6305320963702 21.125392682736376 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1519.0 1 943.0 2 424.5 3 690.0 4 735.0 5 513.0 6 481.5 7 515.0 8 509.0 9 509.0 10 519.5 11 495.0 12 456.0 13 444.0 14 464.5 15 489.5 16 501.5 17 522.5 18 563.0 19 630.0 20 680.5 21 819.5 22 1054.0 23 1333.5 24 1723.0 25 2370.0 26 3680.5 27 5697.5 28 7706.0 29 9725.0 30 12300.0 31 15496.5 32 23131.0 33 35239.5 34 51773.0 35 78885.5 36 125075.0 37 186789.0 38 236884.0 39 243179.5 40 222312.0 41 196351.5 42 169999.5 43 157733.5 44 140693.0 45 120539.5 46 115803.0 47 104169.5 48 79102.5 49 60229.5 50 48819.0 51 41204.5 52 35145.0 53 28870.0 54 23001.5 55 16730.5 56 11730.5 57 8737.5 58 6955.0 59 5521.0 60 4239.0 61 3300.5 62 2497.5 63 1804.5 64 1295.0 65 900.5 66 645.0 67 492.5 68 370.0 69 271.0 70 189.5 71 136.5 72 104.0 73 74.0 74 53.5 75 39.0 76 24.5 77 16.5 78 17.5 79 13.5 80 7.5 81 4.5 82 3.5 83 3.5 84 3.0 85 2.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18488741455946175 2 0.0 3 0.0 4 0.009831642503466404 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.002232758507466607 12-13 0.0 14-15 0.0 16-17 3.0020282453332536E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.001913793006399949 40-41 0.00636054734479983 42-43 0.0026830627442665953 44-45 0.0012946246807999655 46-47 0.007111054406133144 48-49 0.008593305852266439 50-51 0.0060228191671998395 52-53 0.006454360727466494 54-55 0.01979462374266614 56-57 0.022627787899199395 58-59 0.029100911303199223 60-61 0.026680526030399287 62-63 0.010844827036266378 64-65 0.016173427171732903 66-67 0.024691682317866007 68-69 0.0275998971805326 70-71 0.02960750356959921 72-73 0.023603447078932704 74-75 0.03386663114266576 76-77 0.03033924795439919 78-79 0.021670891395999422 80-81 0.009812879826933072 82-83 0.0017449289175999535 84-85 0.007936612173599788 86-87 0.02853803100719924 88-89 0.02649289926506596 90-91 0.022365110427732735 92-93 0.04608113356586544 94-95 0.04763843571813207 96-97 0.03576166147253238 98-99 0.04326673208586552 100-101 0.042666326436798865 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2664865.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.396159355864086 #Duplication Level Percentage of deduplicated Percentage of total 1 85.56313458537002 32.852957505271135 2 6.0279763671441895 4.62902282372502 3 2.4885535078315364 2.8665269115688274 4 1.2888841860625486 1.9795281039724315 5 0.840334309416981 1.6132805028287203 6 0.5183636688116845 1.1941904419190275 7 0.3914126123754373 1.0520118727063645 8 0.2960259175157087 0.9093006641919223 9 0.2636216531596503 0.9109853103936839 >10 1.8621487094044424 14.023365637120843 >50 0.21571133963588104 5.705071214127995 >100 0.2034073052977295 17.8416506619485 >500 0.03286901484847784 8.28502316669835 >1k 0.00696470658302378 4.449466050820914 >5k 4.934304523887394E-4 1.0216730593709726 >10k+ 9.86860902346319E-5 0.6659460733349611 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 17575 0.6595080801466491 TruSeq Adapter, Index 6 (97% over 36bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5697 0.2137819364207943 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5466 0.2051135798623945 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5455 0.2047008009786612 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5236 0.19648274865706142 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5109 0.1917170288175949 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 4306 0.16158417030506236 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 4215 0.15816936317599578 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3888 0.14589857272319612 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3848 0.14439755860052947 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT 3613 0.13557910062986306 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG 3256 0.12218254958506342 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 3089 0.11591581562293024 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.37728177599991E-4 0.0 0.0 2 3.7525353066665665E-4 0.0 0.0 3 4.8782958986665363E-4 0.0 0.0 4 5.62880295999985E-4 0.0 0.0 5 6.004056490666506E-4 0.0 0.0 6 6.75456355199982E-4 0.0 0.0 7 7.88032414399979E-4 0.0 0.0 8 8.630831205333103E-4 0.0 0.0 9 0.0010507098858666387 0.0 0.0 10-11 0.0016135901818666236 0.0 0.0 12-13 0.0022327585074666074 0.0 0.0 14-15 0.0032834683933332456 0.0 0.0 16-17 0.004746957162933206 0.0 0.0 18-19 0.0056850909895998485 0.0 0.0 20-21 0.006792088905066485 0.0 0.0 22-23 0.008368153733866443 0.0 0.0 24-25 0.009775354473866404 0.0 0.0 26-27 0.011501520714933027 0.0 0.0 28-29 0.013321500338666312 0.0 0.0 30-31 0.02060141883359945 0.0 0.0 32-33 0.022515211839999397 0.0 0.0 34-35 0.02476673302399934 0.0 0.0 36-37 0.027224643649865942 0.0 3.7525353066665663E-5 38-39 0.02955121553999921 0.0 3.7525353066665663E-5 40-41 0.03279715858026579 0.0 3.7525353066665663E-5 42-43 0.03621196570933237 0.0 7.505070613333133E-5 44-45 0.038707401688265634 0.0 7.505070613333133E-5 46-47 0.04255375037759886 0.0 7.505070613333133E-5 48-49 0.045855981447465444 3.7525353066665663E-5 7.505070613333133E-5 50-51 0.04919573787039869 3.7525353066665663E-5 7.505070613333133E-5 52-53 0.052554256969865265 3.7525353066665663E-5 7.505070613333133E-5 54-55 0.057488840898131804 3.7525353066665663E-5 7.505070613333133E-5 56-57 0.062742390327465 3.7525353066665663E-5 7.505070613333133E-5 58-59 0.06842748131706484 3.7525353066665663E-5 7.505070613333133E-5 60-61 0.07490060472106466 3.7525353066665663E-5 7.505070613333133E-5 62-63 0.08238691265786446 3.7525353066665663E-5 7.505070613333133E-5 64-65 0.09180577627759756 3.7525353066665663E-5 7.505070613333133E-5 66-67 0.10146855469226396 3.7525353066665663E-5 7.505070613333133E-5 68-69 0.11396449726346362 3.7525353066665663E-5 7.505070613333133E-5 70-71 0.12719218421946327 3.7525353066665663E-5 7.505070613333133E-5 72-73 0.1419021226215962 3.7525353066665663E-5 1.12576059199997E-4 74-75 0.1592575984149291 3.7525353066665663E-5 1.5010141226666265E-4 76-77 0.17848934186159526 3.7525353066665663E-5 1.5010141226666265E-4 78-79 0.20166124738026128 3.7525353066665663E-5 1.5010141226666265E-4 80-81 0.22667189519919395 3.7525353066665663E-5 1.5010141226666265E-4 82-83 0.25868102136505977 3.7525353066665663E-5 1.5010141226666265E-4 84-85 0.29592493428372546 3.7525353066665663E-5 1.5010141226666265E-4 86-87 0.3389289888981243 3.7525353066665663E-5 1.5010141226666265E-4 88-89 0.3868113394111897 3.7525353066665663E-5 1.5010141226666265E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGCC 20 0.0020616688 71.36048 1 CGTACCG 35 3.3869492E-6 67.962364 1 GTCGACG 30 1.2916E-4 63.431534 1 TCGGAAG 3400 0.0 59.08838 3 CGGAAGA 3395 0.0 58.755714 4 GATCGGA 3655 0.0 54.537235 1 ATCGGAA 3670 0.0 54.223625 2 GAAGAGC 3730 0.0 53.220066 6 CGATTGG 110 0.0 47.57365 1 GATACGT 40 5.394708E-4 47.490635 7 GGAAGAG 4360 0.0 46.510323 5 TATGCCG 2195 0.0 43.712566 48-49 GCCGTCT 2220 0.0 43.437943 50-51 CGTATGC 2210 0.0 43.410175 46-47 TGCCGTC 2235 0.0 43.358955 50-51 CGGACGG 55 4.919892E-5 43.248775 1 CTCGTAT 2120 0.0 43.124416 44-45 ATGCCGT 2240 0.0 42.94044 48-49 TCGTATG 2230 0.0 42.913956 44-45 TCTCGTA 2130 0.0 42.698185 42-43 >>END_MODULE