FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_34311-1.34100000035764.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_34311-1.34100000035764.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2664865
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT175750.6595080801466491TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56970.2137819364207943No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC54660.2051135798623945No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG54550.2047008009786612No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG52360.19648274865706142No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC51090.1917170288175949No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT43060.16158417030506236No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT42150.15816936317599578No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA38880.14589857272319612No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA38480.14439755860052947No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT36130.13557910062986306No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG32560.12218254958506342No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT30890.11591581562293024No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCC200.002061668871.360481
CGTACCG353.3869492E-667.9623641
GTCGACG301.2916E-463.4315341
TCGGAAG34000.059.088383
CGGAAGA33950.058.7557144
GATCGGA36550.054.5372351
ATCGGAA36700.054.2236252
GAAGAGC37300.053.2200666
CGATTGG1100.047.573651
GATACGT405.394708E-447.4906357
GGAAGAG43600.046.5103235
TATGCCG21950.043.71256648-49
GCCGTCT22200.043.43794350-51
CGTATGC22100.043.41017546-47
TGCCGTC22350.043.35895550-51
CGGACGG554.919892E-543.2487751
CTCGTAT21200.043.12441644-45
ATGCCGT22400.042.9404448-49
TCGTATG22300.042.91395644-45
TCTCGTA21300.042.69818542-43
Produced by FastQC (version 0.11.2)