##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_34310-1.34100000035813.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5767855 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.648958234907084 33.0 33.0 33.0 33.0 33.0 2 32.66939390813396 33.0 33.0 33.0 33.0 33.0 3 32.724972281723446 33.0 33.0 33.0 33.0 33.0 4 36.667685300688035 37.0 37.0 37.0 37.0 37.0 5 36.630775912362566 37.0 37.0 37.0 37.0 37.0 6 36.52608985489406 37.0 37.0 37.0 37.0 37.0 7 36.489092392232465 37.0 37.0 37.0 37.0 37.0 8 36.420084069381076 37.0 37.0 37.0 37.0 37.0 9 36.416480129961656 37.0 37.0 37.0 37.0 37.0 10-11 36.37656511822853 37.0 37.0 37.0 37.0 37.0 12-13 36.35459247848637 37.0 37.0 37.0 37.0 37.0 14-15 38.16543429056382 40.0 37.0 40.0 37.0 40.0 16-17 38.12175444771063 40.0 37.0 40.0 37.0 40.0 18-19 38.150226210610356 40.0 37.0 40.0 37.0 40.0 20-21 38.08233858167378 40.0 37.0 40.0 37.0 40.0 22-23 37.99266928520221 40.0 37.0 40.0 33.0 40.0 24-25 37.953776317192435 40.0 37.0 40.0 33.0 40.0 26-27 37.84502592384864 40.0 37.0 40.0 33.0 40.0 28-29 37.7361936976571 40.0 37.0 40.0 33.0 40.0 30-31 37.705706020695736 40.0 37.0 40.0 33.0 40.0 32-33 37.59292241223123 40.0 37.0 40.0 33.0 40.0 34-35 37.48828082536749 40.0 37.0 40.0 33.0 40.0 36-37 37.37284666483467 40.0 37.0 40.0 33.0 40.0 38-39 37.26984164823838 40.0 37.0 40.0 33.0 40.0 40-41 37.17272790318064 40.0 37.0 40.0 33.0 40.0 42-43 37.03135914477739 37.0 37.0 40.0 33.0 40.0 44-45 36.89737250676378 37.0 37.0 40.0 33.0 40.0 46-47 36.81642317638013 37.0 37.0 40.0 33.0 40.0 48-49 36.593036492768974 37.0 37.0 40.0 33.0 40.0 50-51 36.41888535686144 37.0 37.0 40.0 33.0 40.0 52-53 36.26990805073983 37.0 37.0 40.0 33.0 40.0 54-55 36.0192391278907 37.0 37.0 40.0 33.0 40.0 56-57 35.83950194656419 37.0 37.0 40.0 30.0 40.0 58-59 35.59767079789627 37.0 33.0 40.0 27.0 40.0 60-61 35.33003959010759 37.0 33.0 40.0 27.0 40.0 62-63 35.02726091415266 37.0 33.0 37.0 27.0 40.0 64-65 34.770502032384655 37.0 33.0 37.0 27.0 40.0 66-67 34.4133869523419 37.0 33.0 37.0 27.0 40.0 68-69 34.11087293283205 37.0 33.0 37.0 27.0 38.5 70-71 33.71488360924468 37.0 33.0 37.0 27.0 37.0 72-73 33.44415055510237 37.0 33.0 37.0 27.0 37.0 74-75 33.045024276789206 37.0 33.0 37.0 27.0 37.0 76-77 30.38468737511605 33.0 30.0 33.0 24.5 37.0 78-79 32.77348164959071 33.0 33.0 37.0 27.0 37.0 80-81 33.24790767104929 37.0 33.0 37.0 27.0 37.0 82-83 33.21283926173595 37.0 33.0 37.0 27.0 37.0 84-85 33.074640659309225 37.0 33.0 37.0 27.0 37.0 86-87 32.920202397598416 35.0 33.0 37.0 27.0 37.0 88-89 32.73179275484561 33.0 33.0 37.0 27.0 37.0 90-91 32.48308946740166 33.0 33.0 37.0 22.0 37.0 92-93 32.43074877922555 33.0 33.0 37.0 24.5 37.0 94-95 32.32664118636824 33.0 33.0 37.0 22.0 37.0 96-97 32.147210444784065 33.0 33.0 37.0 22.0 37.0 98-99 31.967177833007245 33.0 33.0 37.0 22.0 37.0 100-101 30.29597978798011 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.1290716425529581 2306 2 0.11688520363200894 2306 3 0.06947289448117999 2306 4 0.07889843230189086 2306 5 0.1760978120265051 2306 6 0.13322862575407868 2306 7 0.1763339211370223 2306 8 0.1866658770155425 2306 9 0.19735163038168224 2306 10-11 0.14339912301260682 2306 12-13 0.2024072446508498 2306 14-15 0.38447346461054366 2306 16-17 0.36868933980342433 2306 18-19 0.37063315612392955 2306 20-21 0.40656867428550925 2306 22-23 0.4305027459213804 2306 24-25 0.3880226514556355 2306 26-27 0.4300632814799883 2306 28-29 0.38669613099811784 2306 30-31 0.3936934871240325 2306 32-33 0.3401044429850444 2306 34-35 0.3998025650975734 2306 36-37 0.4147406595100307 2306 38-39 0.44378105092033593 2306 40-41 0.3778960094992172 2306 42-43 0.3955357977710179 2306 44-45 0.5028383015150766 2306 46-47 0.5412852414786258 2306 48-49 0.5090385177578653 2306 50-51 0.5240397171907105 2306 52-53 0.37023696918961235 2306 54-55 0.46297166177443927 2306 56-57 0.4525948275174869 2306 58-59 0.48793277447870054 2306 60-61 0.47129756514694776 2306 62-63 0.4234611578886174 2306 64-65 0.37095499824109623 2306 66-67 0.3878062700741154 2306 68-69 0.33274499134981994 2306 70-71 0.33534201341201 2306 72-73 0.3562232655889801 2306 74-75 0.3476853992550488 2306 76-77 -0.06407488905619019 2306 78-79 0.4134803072904063 2306 80-81 0.3881488560853441 2306 82-83 0.45691072128920496 2306 84-85 0.4585563107091346 2306 86-87 0.47763587099046845 2306 88-89 0.6225003447016704 2306 90-91 0.6154615141094553 2306 92-93 0.5532112464396803 2306 94-95 0.430494452312864 2306 96-97 0.3676409655428543 2306 98-99 0.289936688958214 2306 100-101 0.15322847194499545 2307 1 0.1552181665174075 2307 2 0.16586505111010297 2307 3 0.1416283756847605 2307 4 0.1371288151215353 2307 5 0.19898769016170803 2307 6 0.21277250400891745 2307 7 0.16036081390062407 2307 8 0.2195673862698726 2307 9 0.2501809624333333 2307 10-11 0.1991983042480001 2307 12-13 0.22797224079528178 2307 14-15 0.40414912695097627 2307 16-17 0.447237209576528 2307 18-19 0.43795569320813854 2307 20-21 0.40751701932001794 2307 22-23 0.42982026388140326 2307 24-25 0.4471845817430946 2307 26-27 0.4189812767722785 2307 28-29 0.5247638289622287 2307 30-31 0.4329284105763591 2307 32-33 0.5280921457031198 2307 34-35 0.4972953608547499 2307 36-37 0.504703221476035 2307 38-39 0.5037171886538516 2307 40-41 0.3800294411531766 2307 42-43 0.4150212846452632 2307 44-45 0.4524528043934879 2307 46-47 0.421697117814098 2307 48-49 0.44827460043926237 2307 50-51 0.37328026177851115 2307 52-53 0.33136191320475916 2307 54-55 0.5902282826183622 2307 56-57 0.5654709305225225 2307 58-59 0.5304928283119423 2307 60-61 0.4456024089630617 2307 62-63 0.3335663489219698 2307 64-65 0.28234350086098203 2307 66-67 0.24810102038151882 2307 68-69 0.2960870734861558 2307 70-71 0.3829288164557809 2307 72-73 0.29824560595877614 2307 74-75 0.3640818783275037 2307 76-77 0.05884117865201688 2307 78-79 0.3699204465306991 2307 80-81 0.56143722483678 2307 82-83 0.4801986764322166 2307 84-85 0.39265064978285125 2307 86-87 0.2812862157682616 2307 88-89 0.5798261124334729 2307 90-91 0.5337549421079046 2307 92-93 0.33790909648057266 2307 94-95 0.422726604701289 2307 96-97 0.3756971573144554 2307 98-99 0.25365644904731965 2307 100-101 0.40295300029211134 2201 1 0.14297519579826456 2201 2 0.17161368143936073 2201 3 0.12583380281247258 2201 4 0.1368376464726282 2201 5 0.1363210503337342 2201 6 0.1353684550794796 2201 7 0.14588071299960603 2201 8 0.13378997611982157 2201 9 0.15988388459003744 2201 10-11 0.1543491260192411 2201 12-13 0.11573967787297335 2201 14-15 0.30904690818657343 2201 16-17 0.33928791536402514 2201 18-19 0.33519586380862876 2201 20-21 0.31459045139791186 2201 22-23 0.37866680068311354 2201 24-25 0.3175184910161235 2201 26-27 0.29939116608294114 2201 28-29 0.339795589906835 2201 30-31 0.25362360459283195 2201 32-33 0.3497624270116688 2201 34-35 0.24199920442134726 2201 36-37 0.2665538713079698 2201 38-39 0.22643943763675622 2201 40-41 0.30848418603966365 2201 42-43 0.26951778835891105 2201 44-45 0.22169806123902447 2201 46-47 0.3440012760800215 2201 48-49 0.38109149171616963 2201 50-51 0.219248223552718 2201 52-53 0.28868206624149906 2201 54-55 0.3894607115032258 2201 56-57 0.3497064575604014 2201 58-59 0.3194982314720747 2201 60-61 0.3382788308717366 2201 62-63 0.3290578380124245 2201 64-65 0.32952585123485534 2201 66-67 0.19380017388591142 2201 68-69 0.20128195285011685 2201 70-71 0.3501827980622281 2201 72-73 0.22923629558376746 2201 74-75 0.2645288830587589 2201 76-77 0.04001235070881748 2201 78-79 0.4243085968093041 2201 80-81 0.45928797578194747 2201 82-83 0.4488973444994926 2201 84-85 0.45174212557878946 2201 86-87 0.3533534991319982 2201 88-89 0.49698597915750753 2201 90-91 0.6055062466323946 2201 92-93 0.5119305802329421 2201 94-95 0.3086158011429134 2201 96-97 0.3779455222995054 2201 98-99 0.3853029078919761 2201 100-101 0.42878649395544954 1101 1 0.10989569401515098 1101 2 0.13999029770175753 1101 3 0.12305464667281996 1101 4 0.10696592996131926 1101 5 0.19660844529131793 1101 6 0.15043805584672043 1101 7 0.18018088732393522 1101 8 0.16363907093242602 1101 9 0.13544360007721679 1101 10-11 0.19234127515396438 1101 12-13 0.1538955740318002 1101 14-15 0.273591844085054 1101 16-17 0.25697829671712213 1101 18-19 0.1554612617671367 1101 20-21 0.22687303995440544 1101 22-23 0.23493072775794843 1101 24-25 0.33558385778697897 1101 26-27 0.28093403692321317 1101 28-29 0.28634718879477106 1101 30-31 0.42115436809172735 1101 32-33 0.21712650929123356 1101 34-35 0.26703894028698727 1101 36-37 0.3597711004291 1101 38-39 0.3591983312653184 1101 40-41 0.28626158521161216 1101 42-43 0.351460301322021 1101 44-45 0.3517306852306632 1101 46-47 0.42186393255366994 1101 48-49 0.5324535380428514 1101 50-51 0.2672093719683346 1101 52-53 0.3641747448328303 1101 54-55 0.4816281139666714 1101 56-57 0.3359387639490734 1101 58-59 0.3933959856532425 1101 60-61 0.36431721061860145 1101 62-63 0.33424644169280526 1101 64-65 0.33648334870706975 1101 66-67 0.34656486018376853 1101 68-69 0.32190115796242225 1101 70-71 0.3992402943276332 1101 72-73 0.3101150207989818 1101 74-75 0.35575717740871227 1101 76-77 0.0645457959201714 1101 78-79 0.43137487247079065 1101 80-81 0.506276083536001 1101 82-83 0.3456108565395155 1101 84-85 0.3555746341429682 1101 86-87 0.39875027923133644 1101 88-89 0.4610822671402488 1101 90-91 0.5045445641459168 1101 92-93 0.39957189190339193 1101 94-95 0.23477663621487466 1101 96-97 0.36002821407341656 1101 98-99 0.29025892927468533 1101 100-101 0.24332112467633138 2304 1 0.15919479800324154 2304 2 0.15624862532600758 2304 3 0.09205899996368316 2304 4 0.12444317902673419 2304 5 0.13280765015492335 2304 6 0.0812991659089164 2304 7 0.15274814216137145 2304 8 0.10990360213101269 2304 9 0.1496041485810764 2304 10-11 0.1371175958410049 2304 12-13 0.12021350991381752 2304 14-15 0.29607079239040246 2304 16-17 0.24228542759095717 2304 18-19 0.26978321109202597 2304 20-21 0.2439795849950528 2304 22-23 0.24734866525828636 2304 24-25 0.24967934967647665 2304 26-27 0.25878306889589453 2304 28-29 0.21159498113147635 2304 30-31 0.2201654643981712 2304 32-33 0.30137914041591785 2304 34-35 0.4319943554679426 2304 36-37 0.4068240789539459 2304 38-39 0.3448650536742406 2304 40-41 0.3307130543798422 2304 42-43 0.3124089626985693 2304 44-45 0.3369983290052403 2304 46-47 0.420981406673981 2304 48-49 0.36829369081261376 2304 50-51 0.28938364385751214 2304 52-53 0.310203755983089 2304 54-55 0.5412364796215243 2304 56-57 0.47301855270387705 2304 58-59 0.4180389564640805 2304 60-61 0.4096423588300837 2304 62-63 0.2993410327154251 2304 64-65 0.2987321713765496 2304 66-67 0.3688956779227581 2304 68-69 0.3616043573884653 2304 70-71 0.4254889659535692 2304 72-73 0.24984821315833727 2304 74-75 0.39977385693205747 2304 76-77 0.3287258182530408 2304 78-79 0.4335188277619011 2304 80-81 0.35887743106024317 2304 82-83 0.3562336414588927 2304 84-85 0.4775098256715893 2304 86-87 0.36300657855649376 2304 88-89 0.3286029412260305 2304 90-91 0.464574772855741 2304 92-93 0.3233020202364685 2304 94-95 0.20065967456375233 2304 96-97 0.21139909611380148 2304 98-99 0.07799235898819035 2304 100-101 0.19862593595280842 2202 1 0.10643574781560972 2202 2 0.1608529801321481 2202 3 0.12412810267209551 2202 4 0.04344158560992639 2202 5 0.1487660093954446 2202 6 0.12045231585604199 2202 7 0.10615779422871441 2202 8 0.14099558246621058 2202 9 0.13279338132188911 2202 10-11 0.10544478808864 2202 12-13 0.11321761041303802 2202 14-15 0.18529239728301405 2202 16-17 0.21129802897885241 2202 18-19 0.17849984080555004 2202 20-21 0.17681588690855676 2202 22-23 0.1928222219365452 2202 24-25 0.15668134694548286 2202 26-27 0.09783861456618581 2202 28-29 0.2467824225279216 2202 30-31 0.236776444779359 2202 32-33 0.2571133416982505 2202 34-35 0.33747622910219377 2202 36-37 0.25819925563927626 2202 38-39 0.25531161023454274 2202 40-41 0.2245080853984902 2202 42-43 0.2521031946096812 2202 44-45 0.18101233987726317 2202 46-47 0.2906014257469991 2202 48-49 0.3946100390291605 2202 50-51 0.21083579237115657 2202 52-53 0.2009022003524521 2202 54-55 0.29535001406792105 2202 56-57 0.3180299585293511 2202 58-59 0.3829406824323698 2202 60-61 0.3651187344765958 2202 62-63 0.20938553642707092 2202 64-65 0.29005234819183556 2202 66-67 0.34021641453315254 2202 68-69 0.33999728557675013 2202 70-71 0.35487061227101435 2202 72-73 0.23058690279793126 2202 74-75 0.28894288106922517 2202 76-77 -0.050713606917348386 2202 78-79 0.3989466611748682 2202 80-81 0.4570503597683384 2202 82-83 0.4076089780856833 2202 84-85 0.3660050964024961 2202 86-87 0.3407330106823423 2202 88-89 0.4119985784228106 2202 90-91 0.45573104965842504 2202 92-93 0.46011768570290457 2202 94-95 0.4165722505609395 2202 96-97 0.263029659327664 2202 98-99 0.03450729452178791 2202 100-101 0.2636157296883006 1102 1 0.09596996042920125 1102 2 0.12130251060114716 1102 3 0.0647100592341161 1102 4 0.030989563683370136 1102 5 0.08652763540686692 1102 6 0.03526027994546865 1102 7 0.06208668416397245 1102 8 0.09153280020067456 1102 9 0.1007115331019719 1102 10-11 0.059638897251019785 1102 12-13 0.0843076163712908 1102 14-15 0.20277534320525348 1102 16-17 0.1632867358551664 1102 18-19 0.10587091388983083 1102 20-21 0.06040048603194492 1102 22-23 0.11932803338303444 1102 24-25 0.17944256251922752 1102 26-27 0.11293831432298873 1102 28-29 0.17682886584827173 1102 30-31 0.17007739001098088 1102 32-33 0.22090933975533744 1102 34-35 0.1740405422965452 1102 36-37 0.23477239529781002 1102 38-39 0.17236669261450288 1102 40-41 0.2372352760993408 1102 42-43 0.15431169291792912 1102 44-45 0.1667331046701861 1102 46-47 0.23042661216002358 1102 48-49 0.16449778002872506 1102 50-51 0.10173716206779915 1102 52-53 0.1748513958609763 1102 54-55 0.2507576780261971 1102 56-57 0.3374826505262263 1102 58-59 0.1857138369249256 1102 60-61 0.11131699477355994 1102 62-63 0.16562206789030398 1102 64-65 0.17389594506898476 1102 66-67 0.26822945472459736 1102 68-69 0.15228383650870114 1102 70-71 0.1389158576660634 1102 72-73 0.05558131763368124 1102 74-75 0.1103907819624581 1102 76-77 -0.14421823829015779 1102 78-79 0.24322210496854524 1102 80-81 0.36191200716434935 1102 82-83 0.2393285767724933 1102 84-85 0.19472390640659398 1102 86-87 0.21515682004147152 1102 88-89 0.25265195636973914 1102 90-91 0.3990211479819692 1102 92-93 0.29514338044617716 1102 94-95 0.1314088892682932 1102 96-97 0.017558777509592005 1102 98-99 0.006645578482959991 1102 100-101 -0.018703530027025295 2305 1 0.09801747119305304 2305 2 0.09983427098114106 2305 3 0.062042973910322985 2305 4 0.042484324816037145 2305 5 0.12884028302229922 2305 6 0.03069289589932822 2305 7 0.019744580407767387 2305 8 0.019001585274246224 2305 9 0.05461912705985128 2305 10-11 0.051444648627736456 2305 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-1.4017974624752831 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 21.0 9 73.0 10 69.0 11 47.0 12 71.0 13 171.0 14 504.0 15 1216.0 16 2468.0 17 4035.0 18 5916.0 19 8190.0 20 10868.0 21 14040.0 22 18258.0 23 23164.0 24 29694.0 25 37726.0 26 48628.0 27 63408.0 28 83354.0 29 106430.0 30 119736.0 31 146975.0 32 197978.0 33 281158.0 34 432398.0 35 750705.0 36 1393155.0 37 1721708.0 38 265638.0 39 52.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.9757598074124 24.715860339251535 22.921519857990962 15.386859995345104 2 31.63508791396455 21.936577809254914 32.53219784477939 13.896136432001152 3 39.4916307708845 18.79775063693522 24.86419994954797 16.84641864263231 4 31.22696516529596 18.256229201893696 32.83626523922937 17.68054039358097 5 34.32636569400583 16.89400999158266 31.30049212402184 17.479132190389667 6 28.081912600091368 17.765165733188507 36.41766306538566 17.735258601334465 7 34.5416797058872 18.62624840603656 27.48883250359102 19.34323938448522 8 30.124387662311207 25.936695704035557 24.91314362098215 19.02577301267109 9 31.669138700608805 23.602292359984776 24.86385320019314 19.864715739213278 10-11 26.173187696768046 27.97291450467614 28.350332925229853 17.503564873325956 12-13 25.866366266142265 26.24646424017247 27.943429576506347 19.943739917178917 14-15 25.5142509650468 26.713353577716497 27.7120524007625 20.0603430564742 16-17 24.855918554486205 27.094281447022162 28.539850722060795 19.509949276430838 18-19 23.441600040222927 27.213773577872537 29.1735315814978 20.171094800406735 20-21 23.367647071571668 27.858449978371507 28.976933366043355 19.796969584013468 22-23 23.237598726042872 28.439627903267333 28.444040288807535 19.87873308188226 24-25 23.477583954520355 26.788606856448364 29.473721166707556 20.260088022323725 26-27 22.662263527775924 26.685258211241443 29.34740037674317 21.305077884239463 28-29 22.419495635725934 27.890784355709357 28.310836524149792 21.378883484414917 30-31 20.979558258659413 28.472205005153562 28.78232029064531 21.765916445541713 32-33 21.02779976265007 28.053964602092112 28.853568614328896 22.064667020928923 34-35 21.991721531810484 28.119658704982935 29.000644867168546 20.887974896038035 36-37 21.310972623271564 29.326942164808234 29.307203457784563 20.054881754135636 38-39 21.290150991863694 28.569143690548515 28.77948648063848 21.361218836949313 40-41 21.749973797155043 27.815535785977417 27.775509802464793 22.65898061440275 42-43 22.392830748891996 28.4430891930258 28.29830846418083 20.865771593901368 44-45 21.285752081911642 28.62589497234289 28.26817476961657 21.820178176128895 46-47 22.04633982195104 28.3981237142842 28.424599847575845 21.130936616188915 48-49 22.131357810459836 28.286574269497727 28.69747109803413 20.884596822008312 50-51 21.774364695140704 28.291911325891462 27.569524369636582 22.364199609331248 52-53 21.086049485805912 28.453332760002997 27.845596823010148 22.61502093118094 54-55 22.0280581854553 28.488181144419382 28.262232689799305 21.22152798032601 56-57 21.99356645539438 28.25620594279174 27.523102667926786 22.227124933887094 58-59 21.364778576284355 27.920346078608194 28.17309826717907 22.541777077928383 60-61 21.076979464448836 27.882161436712536 28.918411952522664 22.122447146315967 62-63 20.822806242753277 27.92953954067987 28.772651776067782 22.475002440499072 64-65 21.875923089374602 28.434139569942985 28.269824784360686 21.420112556321726 66-67 22.164251180921642 28.683049639591186 27.76858158389124 21.38411759559593 68-69 21.82200902439333 27.85027186075957 28.658241032358134 21.66947808248897 70-71 21.39603535165441 29.244343342490374 28.4030753854789 20.956545920376314 72-73 22.36567075725919 29.50852975810466 27.693233046720465 20.432566437915685 74-75 23.385908925126696 28.427326381488044 27.449914993755687 20.736849699629573 76-77 23.42168277703952 27.169931735953195 27.89612765745279 21.512257829554496 78-79 23.096662472745365 27.402519942368915 28.587612680746343 20.913204904139377 80-81 22.206671012412947 28.452599042658505 28.232046205819316 21.108683739109228 82-83 21.34889327880955 28.417749149669596 28.56700991962561 21.666347651895244 84-85 21.5566723477248 27.915642918838024 28.770393789310855 21.75729094412632 86-87 20.771303807961374 28.561331797986245 29.232268648744263 21.435095745308118 88-89 20.77094365304579 29.10407105480407 29.10014318097258 21.02484211117756 90-91 21.431308215480048 28.136323424868277 28.41117882881598 22.0211895308357 92-93 21.357100312451976 28.568038872120173 27.63906922821552 22.435791587212332 94-95 21.117476335660054 29.55025937651635 27.15791591630397 22.174348371519624 96-97 21.103403482437578 30.38555342089072 26.441403883933777 22.069639212737922 98-99 21.341261838035248 29.008923036683903 27.33882389915508 22.31099122612577 100-101 21.962121978824683 29.03888252492159 27.09136977523416 21.90762572101957 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 161.0 1 114.0 2 132.5 3 287.0 4 295.5 5 192.0 6 177.0 7 182.0 8 184.5 9 191.0 10 187.5 11 178.5 12 179.0 13 188.5 14 193.5 15 201.0 16 220.5 17 245.0 18 299.5 19 354.0 20 393.5 21 475.5 22 605.5 23 796.5 24 1090.0 25 1495.0 26 2047.5 27 2797.5 28 3754.5 29 5043.5 30 6841.5 31 9766.0 32 15735.0 33 27548.5 34 46837.5 35 80156.0 36 126353.0 37 192388.0 38 277678.5 39 331253.0 40 351601.5 41 344330.0 42 354010.0 43 398352.5 44 396257.5 45 377321.0 46 391928.5 47 376574.0 48 311512.5 49 253840.5 50 206133.5 51 170770.0 52 153105.5 53 138861.0 54 110604.5 55 69943.5 56 44696.5 57 35732.0 58 31763.5 59 27627.0 60 22121.0 61 17399.5 62 12524.0 63 8409.5 64 5963.5 65 4402.5 66 3527.5 67 2985.5 68 2441.5 69 1794.0 70 1235.0 71 870.0 72 637.0 73 498.0 74 330.5 75 185.0 76 130.5 77 102.0 78 71.5 79 46.0 80 21.5 81 15.5 82 12.0 83 6.5 84 5.5 85 6.0 86 3.5 87 0.5 88 0.0 89 1.5 90 2.5 91 1.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18160997459194103 2 0.0 3 0.0 4 0.008720746273961464 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.002054489927364679 12-13 0.0 14-15 0.0 16-17 4.0743049192464096E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 8.668733870737041E-6 36-37 0.0 38-39 0.0022018584031672085 40-41 0.00609411991112814 42-43 0.002904025846696909 44-45 0.0013696599515764525 46-47 0.006874305959494474 48-49 0.008157278572363556 50-51 0.005357277532115491 52-53 0.006640250144984573 54-55 0.01825635353177221 56-57 0.020891648628476273 58-59 0.026613012983162716 60-61 0.024974622281593417 62-63 0.010263780902952657 64-65 0.01517895300766056 66-67 0.022165952507474617 68-69 0.02572880212834754 70-71 0.028060691539575803 72-73 0.02166316594297187 74-75 0.03134614167658514 76-77 0.02847679076537118 78-79 0.020380193330102787 80-81 0.009180189169110528 82-83 0.0016817343709229862 84-85 0.007689166943343756 86-87 0.026266263628333238 88-89 0.023865024346139074 90-91 0.020570905475259003 92-93 0.04315295720852899 94-95 0.04486069778106419 96-97 0.03333128173298393 98-99 0.04037896236989314 100-101 0.04024893136183209 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5767855.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.951014019022555 #Duplication Level Percentage of deduplicated Percentage of total 1 81.82967543016845 24.50881755981041 2 8.155401660338603 4.885250989191226 3 2.8645683864651796 2.5739018370440223 4 1.1571580956966703 1.3863223338574566 5 0.8711944842972618 1.3046579106241207 6 0.7727423356120442 1.388664991620514 7 0.4848145803972467 1.0164481805873131 8 0.47655903748158995 1.1418741130002397 9 0.31219273529240604 0.8415440092241865 >10 2.4004194348190366 14.481036466104333 >50 0.317673192404591 6.663050902477398 >100 0.3002533413287902 18.64270883974804 >500 0.0358303305332094 7.490037001411452 >1k 0.020760175484446714 10.751163514809534 >5k 4.6571820356306127E-4 0.9335117982513501 >10k+ 2.910614770973664E-4 1.9910095522385152 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 40962 0.7101773536262613 TruSeq Adapter, Index 7 (97% over 38bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 24492 0.42462925992418327 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 24331 0.4218379276178059 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 13250 0.2297214475745316 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11160 0.19348613999485076 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 8081 0.14010407681885206 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8044 0.13946259051241752 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7394 0.12819323648045938 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 7170 0.12430964370636918 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 6234 0.10808177390034944 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6058 0.10503037957785 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.987617580539039E-4 0.0 0.0 2 4.8544909676127433E-4 0.0 0.0 3 5.721364354686448E-4 0.0 0.0 4 5.894739032101189E-4 0.0 0.0 5 6.761612419174893E-4 0.0 0.0 6 7.801860483663337E-4 0.0 0.0 7 9.362232580396006E-4 0.0 0.0 8 0.0010922604677128672 0.0 0.0 9 0.0011962852741617118 0.0 0.0 10-11 0.001889783983820675 0.0 0.0 12-13 0.002878019645084698 0.0 0.0 14-15 0.004195667193436729 0.0 0.0 16-17 0.005712695620815711 0.0 0.0 18-19 0.007411767459480171 0.0 0.0 20-21 0.009448919919103375 0.0 0.0 22-23 0.01210155248354891 0.0 0.0 24-25 0.015135609338306873 0.0 0.0 26-27 0.017770904435010934 0.0 0.0 28-29 0.020908986096217747 0.0 0.0 30-31 0.03320125072492287 0.0 0.0 32-33 0.03789970448286235 0.0 0.0 34-35 0.04212137787791129 0.0 0.0 36-37 0.04686317530520445 0.0 0.0 38-39 0.05268856446633974 0.0 0.0 40-41 0.059034077659719256 0.0 0.0 42-43 0.06589971488534299 0.0 0.0 44-45 0.07326813867546947 0.0 0.0 46-47 0.08107866789300355 0.0 0.0 48-49 0.09000746377986271 0.0 0.0 50-51 0.10060065656990336 0.0 0.0 52-53 0.11193936047282742 0.0 0.0 54-55 0.12487311140796709 0.0 0.0 56-57 0.13948859671402974 0.0 0.0 58-59 0.15629727168938884 0.0 0.0 60-61 0.1747183311647051 0.0 0.0 62-63 0.1954799487851203 0.0 0.0 64-65 0.2188595240344981 0.0 0.0 66-67 0.24516046259831428 0.0 0.0 68-69 0.2743914332104396 0.0 0.0 70-71 0.30716791597569637 0.0 0.0 72-73 0.34512830159565383 0.0 0.0 74-75 0.38659952443325984 0.0 1.7337467741474083E-5 76-77 0.43344536227072283 0.0 1.7337467741474083E-5 78-79 0.4869487877209118 0.0 1.7337467741474083E-5 80-81 0.5474825563402687 0.0 1.7337467741474083E-5 82-83 0.6147692686449295 0.0 1.7337467741474083E-5 84-85 0.6903259530622736 0.0 1.7337467741474083E-5 86-87 0.7748547770358305 0.0 1.7337467741474083E-5 88-89 0.8654517147189034 0.0 1.7337467741474083E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTGTT 63620 0.0 45.77096 1 CGAGTGG 230 0.0 43.44339 1 TATGCCG 5590 0.0 41.764313 48-49 GCCGTCT 5675 0.0 41.43315 50-51 ATGCCGT 5650 0.0 41.40487 48-49 CGGAAGA 11375 0.0 41.377037 4 TCGTATG 5670 0.0 41.29748 44-45 TGCCGTC 5680 0.0 41.229424 50-51 CGTATGC 5650 0.0 41.149086 46-47 TCGGAAG 11575 0.0 40.662098 3 GAAGAGC 11650 0.0 40.56234 6 CTCGTAT 5675 0.0 40.50785 44-45 TCTCGTA 5685 0.0 40.43642 42-43 CATCTCG 5790 0.0 39.580246 40-41 ATCTCGT 5785 0.0 39.53218 42-43 CTGCTTG 6015 0.0 39.09486 58-59 CTTGAAA 5785 0.0 38.101337 62-63 GTCGCGC 75 6.846736E-6 38.06469 1 CGACTCG 50 0.0016029052 38.064686 1 GATCGGA 12395 0.0 38.003265 1 >>END_MODULE