FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_34310-1.34100000035813.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_34310-1.34100000035813.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5767855
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT409620.7101773536262613TruSeq Adapter, Index 7 (97% over 38bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA244920.42462925992418327No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC243310.4218379276178059No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT132500.2297214475745316No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC111600.19348613999485076No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT80810.14010407681885206No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC80440.13946259051241752No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC73940.12819323648045938No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA71700.12430964370636918No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT62340.10808177390034944No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG60580.10503037957785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGTT636200.045.770961
CGAGTGG2300.043.443391
TATGCCG55900.041.76431348-49
GCCGTCT56750.041.4331550-51
ATGCCGT56500.041.4048748-49
CGGAAGA113750.041.3770374
TCGTATG56700.041.2974844-45
TGCCGTC56800.041.22942450-51
CGTATGC56500.041.14908646-47
TCGGAAG115750.040.6620983
GAAGAGC116500.040.562346
CTCGTAT56750.040.5078544-45
TCTCGTA56850.040.4364242-43
CATCTCG57900.039.58024640-41
ATCTCGT57850.039.5321842-43
CTGCTTG60150.039.0948658-59
CTTGAAA57850.038.10133762-63
GTCGCGC756.846736E-638.064691
CGACTCG500.001602905238.0646861
GATCGGA123950.038.0032651
Produced by FastQC (version 0.11.2)