##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_34302-1.341000000358ea.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8369637 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64444419752016 33.0 33.0 33.0 33.0 33.0 2 32.65973840920461 33.0 33.0 33.0 33.0 33.0 3 32.719751406184045 33.0 33.0 33.0 33.0 33.0 4 36.659491206129964 37.0 37.0 37.0 37.0 37.0 5 36.626817387659706 37.0 37.0 37.0 37.0 37.0 6 36.52997125203877 37.0 37.0 37.0 37.0 37.0 7 36.445029336397745 37.0 37.0 37.0 37.0 37.0 8 36.39271667337544 37.0 37.0 37.0 37.0 37.0 9 36.441874241379885 37.0 37.0 37.0 37.0 37.0 10-11 36.378824075643905 37.0 37.0 37.0 37.0 37.0 12-13 36.36063493554141 37.0 37.0 37.0 37.0 37.0 14-15 38.21163958484699 40.0 37.0 40.0 37.0 40.0 16-17 38.227398870464754 40.0 37.0 40.0 37.0 40.0 18-19 38.19686110640163 40.0 37.0 40.0 37.0 40.0 20-21 38.193965461106615 40.0 37.0 40.0 37.0 40.0 22-23 38.0310594115372 40.0 37.0 40.0 35.0 40.0 24-25 38.05376386096553 40.0 37.0 40.0 35.0 40.0 26-27 37.99409819087734 40.0 37.0 40.0 35.0 40.0 28-29 37.83445028739 40.0 37.0 40.0 33.0 40.0 30-31 37.83460608865116 40.0 37.0 40.0 33.0 40.0 32-33 37.69917548395468 40.0 37.0 40.0 33.0 40.0 34-35 37.54292928116237 40.0 37.0 40.0 33.0 40.0 36-37 37.28358995736613 40.0 37.0 40.0 33.0 40.0 38-39 37.4139980025418 40.0 37.0 40.0 33.0 40.0 40-41 37.20211844312961 40.0 37.0 40.0 33.0 40.0 42-43 37.02274429583983 37.0 37.0 40.0 33.0 40.0 44-45 37.03322019819976 37.0 37.0 40.0 33.0 40.0 46-47 37.049431773444894 37.0 37.0 40.0 33.0 40.0 48-49 36.74085172391587 37.0 37.0 40.0 33.0 40.0 50-51 36.64468739803172 37.0 37.0 40.0 33.0 40.0 52-53 36.57706218322252 37.0 37.0 40.0 33.0 40.0 54-55 36.240555175809895 37.0 37.0 40.0 33.0 40.0 56-57 36.15099376472361 37.0 37.0 40.0 33.0 40.0 58-59 35.90707852682261 37.0 37.0 40.0 33.0 40.0 60-61 35.628663405593336 37.0 35.0 40.0 27.0 40.0 62-63 35.36603128666154 37.0 33.0 38.5 27.0 40.0 64-65 35.113836418473106 37.0 33.0 37.0 27.0 40.0 66-67 34.56542422329666 37.0 33.0 37.0 27.0 40.0 68-69 34.37562614722718 37.0 33.0 37.0 27.0 40.0 70-71 33.87087265552855 37.0 33.0 37.0 27.0 38.5 72-73 33.26743417904504 37.0 33.0 37.0 24.5 37.0 74-75 32.51687564227696 37.0 33.0 37.0 22.0 37.0 76-77 30.011015173059477 33.0 30.0 35.0 22.0 37.0 78-79 32.0820260783114 35.0 33.0 37.0 22.0 37.0 80-81 32.39376187999551 37.0 33.0 37.0 22.0 37.0 82-83 32.347334358706355 37.0 33.0 37.0 22.0 37.0 84-85 32.2113117331134 37.0 33.0 37.0 22.0 37.0 86-87 32.084789818244204 37.0 33.0 37.0 18.5 37.0 88-89 31.889720545825345 33.0 33.0 37.0 15.0 37.0 90-91 31.63966663070334 33.0 33.0 37.0 15.0 37.0 92-93 31.60654589918296 33.0 33.0 37.0 15.0 37.0 94-95 31.530025913907615 33.0 33.0 37.0 15.0 37.0 96-97 31.374471138951428 33.0 33.0 37.0 15.0 37.0 98-99 31.216253763454734 33.0 33.0 37.0 15.0 37.0 100-101 29.709380705519248 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.15039988201208132 2306 2 0.13523686397672208 2306 3 0.09600343096032304 2306 4 0.13916118422915247 2306 5 0.18726083702453167 2306 6 0.19118227002562804 2306 7 0.15344223963705872 2306 8 0.17883313039763493 2306 9 0.18116407197825168 2306 10-11 0.15547569264439431 2306 12-13 0.1892420243202224 2306 14-15 0.3767143533999402 2306 16-17 0.37519726458062763 2306 18-19 0.3926936920903614 2306 20-21 0.35660723012247075 2306 22-23 0.3993568882865617 2306 24-25 0.3681163019842799 2306 26-27 0.37740822523524997 2306 28-29 0.38723000127834695 2306 30-31 0.40215194852628855 2306 32-33 0.40130309209752113 2306 34-35 0.41179695850780007 2306 36-37 0.40126388351118436 2306 38-39 0.4118066763149173 2306 40-41 0.3982922827899458 2306 42-43 0.34222697017080606 2306 44-45 0.3805805156151507 2306 46-47 0.41552728779097947 2306 48-49 0.48132615346284524 2306 50-51 0.4260853835302427 2306 52-53 0.31789416561545636 2306 54-55 0.3545706200679035 2306 56-57 0.3839206002175146 2306 58-59 0.46512898012219495 2306 60-61 0.338560285203684 2306 62-63 0.2925009590247214 2306 64-65 0.21248751224253937 2306 66-67 0.2795232452954153 2306 68-69 0.35379502001819674 2306 70-71 0.32152104371518675 2306 72-73 0.1289821038127954 2306 74-75 0.16507265597615373 2306 76-77 -0.1646930135623741 2306 78-79 0.40672306506731815 2306 80-81 0.4363357736320168 2306 82-83 0.4377257619689914 2306 84-85 0.4317249961253893 2306 86-87 0.3328495903176858 2306 88-89 0.47525419617204534 2306 90-91 0.5202005277380408 2306 92-93 0.44819479761676106 2306 94-95 0.30300486143594796 2306 96-97 0.3472189471550635 2306 98-99 0.37566993433062024 2306 100-101 0.18010989409038913 2307 1 0.14496089161257686 2307 2 0.16830805229774626 2307 3 0.12554489851504513 2307 4 0.11298557434875534 2307 5 0.18038765255310807 2307 6 0.17771501716292448 2307 7 0.1359295186864955 2307 8 0.18327419260680244 2307 9 0.21961588917289987 2307 10-11 0.19892971983469465 2307 12-13 0.16900365840374576 2307 14-15 0.3248418598584166 2307 16-17 0.3166379416331253 2307 18-19 0.33854887220255137 2307 20-21 0.2909568102722986 2307 22-23 0.40037725944822 2307 24-25 0.3902803532444281 2307 26-27 0.40773154612858065 2307 28-29 0.39244064179487026 2307 30-31 0.3147858713224707 2307 32-33 0.3499731990397521 2307 34-35 0.4364814338518954 2307 36-37 0.4473699789829908 2307 38-39 0.38058558756619476 2307 40-41 0.4860535865991764 2307 42-43 0.4324816978954189 2307 44-45 0.42479297313030884 2307 46-47 0.4661374131972096 2307 48-49 0.5253870719193969 2307 50-51 0.3801366093672911 2307 52-53 0.20330121153240555 2307 54-55 0.35903258168159624 2307 56-57 0.43021589287778284 2307 58-59 0.3742262483519667 2307 60-61 0.3005686714266389 2307 62-63 0.24223122685071274 2307 64-65 0.28288540456331646 2307 66-67 0.16761989511842046 2307 68-69 0.296110825946279 2307 70-71 0.3765984719662825 2307 72-73 0.2774473573889651 2307 74-75 0.2841487405890888 2307 76-77 0.0562111441695734 2307 78-79 0.525756125818198 2307 80-81 0.5739131234797199 2307 82-83 0.5652307013827667 2307 84-85 0.5986315118393861 2307 86-87 0.5328019847573202 2307 88-89 0.6622428100392526 2307 90-91 0.6608706464407845 2307 92-93 0.5972673589772164 2307 94-95 0.578702088111914 2307 96-97 0.5373046910660619 2307 98-99 0.523581096425719 2307 100-101 0.40635315139856587 2201 1 0.13745576654206104 2201 2 0.15489119878731117 2201 3 0.10058594836421975 2201 4 0.13126653984946302 2201 5 0.1411818659857289 2201 6 0.10510796032536263 2201 7 0.09668528979211999 2201 8 0.13035263306326073 2201 9 0.1381320703666873 2201 10-11 0.18250811822694857 2201 12-13 0.16293169629316395 2201 14-15 0.2801790279494938 2201 16-17 0.32198053107801883 2201 18-19 0.33612126332216974 2201 20-21 0.3185220447464232 2201 22-23 0.32057944729113785 2201 24-25 0.2572471848745934 2201 26-27 0.2881669576322494 2201 28-29 0.33288312742025283 2201 30-31 0.29517493197033673 2201 32-33 0.278846859489704 2201 34-35 0.36101425183951363 2201 36-37 0.3247640568473855 2201 38-39 0.2748944383102483 2201 40-41 0.346141098482029 2201 42-43 0.3526731118005415 2201 44-45 0.2842299454795736 2201 46-47 0.3837000665109187 2201 48-49 0.5109083780690895 2201 50-51 0.43259620767362605 2201 52-53 0.39906756975116764 2201 54-55 0.5271761794753758 2201 56-57 0.5359653818607413 2201 58-59 0.4935162389740526 2201 60-61 0.5251266706284525 2201 62-63 0.44724597912235 2201 64-65 0.411041853143324 2201 66-67 0.32283281907573524 2201 68-69 0.3578594612665924 2201 70-71 0.4543857641754201 2201 72-73 0.16707578032058024 2201 74-75 0.32525041928321485 2201 76-77 0.24385093887791953 2201 78-79 0.5409320195220673 2201 80-81 0.5227413545893924 2201 82-83 0.48199055879084796 2201 84-85 0.4674519306979974 2201 86-87 0.4767942421929945 2201 88-89 0.5432360708265449 2201 90-91 0.6067528588757796 2201 92-93 0.4823580170791324 2201 94-95 0.3641596904889681 2201 96-97 0.4392641035792799 2201 98-99 0.29817336591903754 2201 100-101 0.35295753859188395 1101 1 0.12416597643268545 1101 2 0.13509629245501742 1101 3 0.09649667628366387 1101 4 0.08124594365038718 1101 5 0.13352018582299507 1101 6 0.12622102339844332 1101 7 0.15615927663385065 1101 8 0.11164175392853792 1101 9 0.10585734989788165 1101 10-11 0.1224061855728138 1101 12-13 0.1569178179494557 1101 14-15 0.24559949220582666 1101 16-17 0.2103769290198798 1101 18-19 0.23356050453489985 1101 20-21 0.2618847739916532 1101 22-23 0.19983790999931728 1101 24-25 0.2719092430755339 1101 26-27 0.26803848817869635 1101 28-29 0.21541696819310374 1101 30-31 0.27913896433548757 1101 32-33 0.24108947318443086 1101 34-35 0.2105521874967735 1101 36-37 0.23924943248711372 1101 38-39 0.27367116174617934 1101 40-41 0.32678834859824946 1101 42-43 0.28155496586085604 1101 44-45 0.35197455049318194 1101 46-47 0.2847928298854754 1101 48-49 0.3807159915679179 1101 50-51 0.24410604143212566 1101 52-53 0.2706645111361752 1101 54-55 0.390612049053253 1101 56-57 0.29738626545357505 1101 58-59 0.3244120200888716 1101 60-61 0.3524212936968283 1101 62-63 0.3086398599863074 1101 64-65 0.21483229502445056 1101 66-67 0.22982996737578532 1101 68-69 0.3045876306192312 1101 70-71 0.3436637747414366 1101 72-73 0.21489507170544186 1101 74-75 0.2782607999793356 1101 76-77 0.07925109284641962 1101 78-79 0.4095940290121973 1101 80-81 0.5365803590252511 1101 82-83 0.40826029593252144 1101 84-85 0.38344082887017095 1101 86-87 0.38427918684798357 1101 88-89 0.42152169404423034 1101 90-91 0.44583332594886826 1101 92-93 0.3330697772440736 1101 94-95 0.3123902175504689 1101 96-97 0.34672072184222813 1101 98-99 0.30742947780140284 1101 100-101 0.2926401135721086 2304 1 0.1535453962352733 2304 2 0.15356601944856152 2304 3 0.10925994703931963 2304 4 0.09504296690649028 2304 5 0.16854043982762335 2304 6 0.11842342727018007 2304 7 0.15947324814050035 2304 8 0.15438744721340925 2304 9 0.13403017728805366 2304 10-11 0.13165253390412346 2304 12-13 0.12942099144206765 2304 14-15 0.24811631445580673 2304 16-17 0.19870587189857503 2304 18-19 0.23342611044894568 2304 20-21 0.18754164000530693 2304 22-23 0.18446875572139732 2304 24-25 0.20970027700021632 2304 26-27 0.2387007424775831 2304 28-29 0.23734546952198343 2304 30-31 0.1719799406599094 2304 32-33 0.2700075626401315 2304 34-35 0.3533215548017381 2304 36-37 0.35598804078342994 2304 38-39 0.31198983283930204 2304 40-41 0.27463254537200754 2304 42-43 0.3628443703868527 2304 44-45 0.3375371743415698 2304 46-47 0.28705718142862935 2304 48-49 0.323436054302114 2304 50-51 0.2838184666535213 2304 52-53 0.2458575620091068 2304 54-55 0.45946650822124724 2304 56-57 0.37337628949541823 2304 58-59 0.36936477770134246 2304 60-61 0.38294925564935056 2304 62-63 0.2966010338107452 2304 64-65 0.30934095932450134 2304 66-67 0.25781140694402893 2304 68-69 0.26343895542522233 2304 70-71 0.38532772183058484 2304 72-73 0.04285041115567623 2304 74-75 0.09439016421646329 2304 76-77 0.17462141321656333 2304 78-79 0.35045540725494817 2304 80-81 0.43551295419872815 2304 82-83 0.37281408565400653 2304 84-85 0.41623251627606095 2304 86-87 0.3467320931567812 2304 88-89 0.4728260738553445 2304 90-91 0.5832089688648239 2304 92-93 0.2803110691177437 2304 94-95 0.3198028075900048 2304 96-97 0.32691298227679866 2304 98-99 0.16676537653764356 2304 100-101 0.3447165403524721 2202 1 0.11354680175463017 2202 2 0.14692533572807065 2202 3 0.09952333029973204 2202 4 0.09999722501215302 2202 5 0.1299880715849966 2202 6 0.09720764800167814 2202 7 0.12143214115461376 2202 8 0.14621799173575312 2202 9 0.14238232872428824 2202 10-11 0.11850475726902943 2202 12-13 0.1094116791578017 2202 14-15 0.2180375626173472 2202 16-17 0.21376743810127152 2202 18-19 0.14861867342641233 2202 20-21 0.17810322007636614 2202 22-23 0.18006408550250796 2202 24-25 0.21806226383834115 2202 26-27 0.1719498009377034 2202 28-29 0.218127911581675 2202 30-31 0.2414826569913231 2202 32-33 0.26253439074224616 2202 34-35 0.32659150132014503 2202 36-37 0.21840337009890476 2202 38-39 0.27208652948079504 2202 40-41 0.29268099289109983 2202 42-43 0.31387252108554975 2202 44-45 0.3067476148641006 2202 46-47 0.2792604382813977 2202 48-49 0.4764119972144556 2202 50-51 0.3308314811447417 2202 52-53 0.2944108812767041 2202 54-55 0.33776559842770126 2202 56-57 0.4657592315061052 2202 58-59 0.4332427848732294 2202 60-61 0.49729539561608505 2202 62-63 0.3000433436366521 2202 64-65 0.29480052570884396 2202 66-67 0.28993575171764263 2202 68-69 0.21680088980787815 2202 70-71 0.36111122880831203 2202 72-73 0.27050601278262576 2202 74-75 0.28383443005679254 2202 76-77 0.0944752021978097 2202 78-79 0.41830046764486184 2202 80-81 0.5365605619271889 2202 82-83 0.4141433695921961 2202 84-85 0.3816469317884028 2202 86-87 0.4280888400520624 2202 88-89 0.508563163843931 2202 90-91 0.6342086804315556 2202 92-93 0.4140363191147962 2202 94-95 0.44214931496064835 2202 96-97 0.348136369385859 2202 98-99 0.2420958783770324 2202 100-101 0.36581492354533296 1102 1 0.11260038439517928 1102 2 0.13251482727878283 1102 3 0.09904909792647487 1102 4 0.0805463372676627 1102 5 0.12927070776809302 1102 6 0.06761310796672149 1102 7 0.09157482491254854 1102 8 0.05610005056584555 1102 9 0.06642470868890626 1102 10-11 0.10336158882181223 1102 12-13 0.11621894502652452 1102 14-15 0.17417913073546032 1102 16-17 0.13300826393328435 1102 18-19 0.16029545483378627 1102 20-21 0.1490500108724504 1102 22-23 0.15984442083710348 1102 24-25 0.1960659071382338 1102 26-27 0.14629315610661564 1102 28-29 0.1803912685360487 1102 30-31 0.21561332079362927 1102 32-33 0.1598933274234824 1102 34-35 0.18872608270453384 1102 36-37 0.1736436564359778 1102 38-39 0.12510665128544218 1102 40-41 0.20099983785886621 1102 42-43 0.1830173980688059 1102 44-45 0.14941727290426599 1102 46-47 0.20231867651092728 1102 48-49 0.20830402194641806 1102 50-51 0.144777212426483 1102 52-53 0.13045860427857292 1102 54-55 0.21656559745984794 1102 56-57 0.22620612127896322 1102 58-59 0.24520343057443483 1102 60-61 0.11866049889178498 1102 62-63 0.1469085207790286 1102 64-65 0.18573668227500661 1102 66-67 0.2459662025561613 1102 68-69 0.15417391691143933 1102 70-71 0.12291143296832985 1102 72-73 0.09262463402344423 1102 74-75 0.22825644160645453 1102 76-77 -0.025218294145211928 1102 78-79 0.279511931272971 1102 80-81 0.44535079661510935 1102 82-83 0.37685406369887886 1102 84-85 0.3933234255463489 1102 86-87 0.34545179739539833 1102 88-89 0.42304823948363435 1102 90-91 0.5065370750893976 1102 92-93 0.3377385620983908 1102 94-95 0.25080278757698693 1102 96-97 0.13059764470935065 1102 98-99 0.10158032025737285 1102 100-101 0.2705177868533646 2305 1 0.11449960759522071 2305 2 0.128598458720802 2305 3 0.09044153558925672 2305 4 0.07328990673812541 2305 5 0.1044325157199566 2305 6 0.06408309153940905 2305 7 0.08237508092526724 2305 8 0.07289665521751942 2305 9 0.07042670296966946 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sequence quality scores pass #Quality Count 7 11.0 8 190.0 9 574.0 10 393.0 11 175.0 12 157.0 13 320.0 14 680.0 15 1617.0 16 2961.0 17 4989.0 18 7759.0 19 11253.0 20 15802.0 21 21316.0 22 28569.0 23 38363.0 24 49387.0 25 65754.0 26 89638.0 27 132670.0 28 216117.0 29 276081.0 30 197326.0 31 179830.0 32 236631.0 33 335004.0 34 517902.0 35 903468.0 36 1788599.0 37 2642076.0 38 603901.0 39 124.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.84104217278768 24.374496671930164 23.426959137181978 14.35750201810018 2 27.52799195472874 26.838798385162942 32.558556601678184 13.074653058430133 3 34.94961609446145 18.296838919059454 30.814024550885538 15.939520435593563 4 26.497627407364217 18.31288780466355 33.61748444001748 21.572000347954763 5 35.57076609176718 17.415068299855776 31.480493120549912 15.533672487827129 6 29.01170026848237 18.505091678408515 36.924313443940285 15.558894609168833 7 30.35123267592131 24.55001333988559 28.539218606493925 16.559535377699174 8 26.4148492939419 31.359687403408298 25.91685875982435 16.308604542825453 9 34.2812716967295 23.38163530867587 24.63409106034109 17.70300193425354 10-11 26.11122332466312 29.372163085580745 27.547367610461297 16.969245979294836 12-13 23.140681011613765 27.760875411920495 27.20678925501787 21.89165432144787 14-15 22.359058104909447 28.384886943125494 27.655494497551086 21.600560454413973 16-17 24.397216572981502 26.307126239626083 28.504450714880935 20.791206472511476 18-19 20.429535952395547 26.55388160800761 31.731173048484663 21.285409391112182 20-21 23.56500048926853 26.82089438287467 31.32426770718969 18.28983742066711 22-23 20.68225897969052 32.907126079661516 28.12024583622922 18.290369104418748 24-25 20.804635852188095 26.155076976456687 31.513385825454556 21.526901345900665 26-27 20.56047950466669 26.283153020853835 28.34409066964314 24.81227680483634 28-29 23.07083329898298 29.697166077811975 28.026293135532644 19.205707487672406 30-31 19.45806012853365 27.180217015385495 31.089179853319806 22.272543002761054 32-33 19.419444355830485 30.08186615500768 28.519295400744383 21.979394088417457 34-35 22.791294460637566 30.29423081874583 28.58631315708872 18.32816156352788 36-37 19.445628287104924 30.80086985851358 31.68973158573066 18.06377026865084 38-39 19.993228944535595 27.373506151570204 30.759069728429537 21.874195175464667 40-41 23.08355230306853 26.95839357963331 30.62698848636003 19.331065630938127 42-43 20.144583651107588 30.206970359085005 31.04259069126848 18.605855298538927 44-45 19.584914118741086 27.483257405053408 31.25586279071407 21.675965685491438 46-47 22.92936249776408 27.290536934107116 28.223581568906305 21.556518999222494 48-49 20.313081299392245 30.49132130369388 30.535538518296136 18.66005887861774 50-51 23.107995419861375 27.588757511340123 30.013762401306032 19.289484667492474 52-53 19.658584689297765 27.39675261564183 27.426660344979624 25.51800235008079 54-55 22.944884829325414 27.376091636065187 30.367185508610778 19.31183802599862 56-57 19.933593229731297 27.07919259510973 30.045544112048756 22.941670063110216 58-59 19.83056351110582 26.88453321515935 30.656366360379973 22.628536913354854 60-61 22.545052165854425 27.103232634332773 30.852664438560435 19.499050761252366 62-63 19.698467783299968 27.21824595825605 30.667204779204084 22.4160814792399 64-65 23.014435574549942 27.27489073523065 30.395290237185613 19.315383453033792 66-67 20.23486882643386 33.15426762839741 27.031510002997244 19.579353542171486 68-69 20.206266896279693 33.04204651581914 27.081630920147763 19.670055667753402 70-71 19.831392789354766 34.02170391917917 27.03549461626852 19.11140867519756 72-73 19.788881486805707 34.14161572066231 26.870049939861477 19.199452852670507 74-75 20.59451177790003 32.9497403996391 26.844282451598854 19.611465370862014 76-77 20.84776549941883 31.787976339165482 27.420222188877574 19.944035972538117 78-79 20.75621755227696 31.417336977584416 27.910279099265612 19.916166370873015 80-81 20.158358703709357 31.785961951635283 27.714224961773414 20.341454382881945 82-83 19.413452560103757 31.461923881815906 28.146492884568165 20.97813067351217 84-85 19.541590684221088 30.97855313013821 28.37899011271799 21.100866072922717 86-87 19.433370487649352 31.029861349844808 28.92190715715463 20.614861005351212 88-89 19.692797643849367 31.294089611572517 28.527962169077874 20.48515057550024 90-91 20.1268113495644 30.517158323457256 28.163104486772788 21.192925840205557 92-93 19.735195240243964 31.316499272350207 27.863022540708048 21.085282946697784 94-95 19.508108667978792 31.818959059837624 27.682442564137226 20.990489708046354 96-97 19.961408009454555 32.10563417484566 27.14869862439018 20.784259191309612 98-99 20.26424011871131 31.0911726303712 27.139768772564437 21.504818478353048 100-101 20.293309880240773 31.282392980423733 26.823619986270952 21.600677153064538 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 104.0 1 81.0 2 134.0 3 370.0 4 446.0 5 293.0 6 206.5 7 200.5 8 222.5 9 229.5 10 215.0 11 190.0 12 209.0 13 228.0 14 233.0 15 276.0 16 365.5 17 470.5 18 608.0 19 872.0 20 1283.5 21 1887.5 22 2858.0 23 4271.5 24 6200.0 25 8761.0 26 12367.5 27 17235.5 28 22656.5 29 28630.0 30 35596.5 31 43695.0 32 55279.0 33 80359.0 34 123016.5 35 204367.0 36 317861.5 37 439489.5 38 575983.5 39 645449.0 40 650361.5 41 607696.5 42 545204.5 43 538269.5 44 515279.0 45 473541.5 46 461961.5 47 409536.5 48 312661.5 49 252370.5 50 208961.5 51 171337.5 52 148018.5 53 123156.5 54 91523.5 55 61220.5 56 42155.5 57 30715.0 58 23927.0 59 18407.5 60 13628.0 61 10236.5 62 7362.5 63 5190.0 64 3860.0 65 2874.5 66 2100.0 67 1545.5 68 1113.0 69 726.0 70 440.5 71 315.0 72 233.0 73 159.5 74 110.0 75 75.5 76 66.0 77 48.0 78 27.0 79 18.0 80 13.0 81 10.5 82 9.0 83 5.0 84 3.5 85 3.5 86 2.5 87 1.5 88 1.0 89 1.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18214648974621 2 0.0 3 0.0 4 0.008757846965167067 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0021327089812855685 12-13 0.0 14-15 0.0 16-17 3.166206610872132E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 5.973974737494589E-6 36-37 0.0 38-39 0.00225218847603546 40-41 0.006212933726994373 42-43 0.0027002365813475543 44-45 0.0013859621390987446 46-47 0.0069776024933936795 48-49 0.00865628939462966 50-51 0.005472160859545044 52-53 0.006726695554418908 54-55 0.018853864271532923 56-57 0.02169150227184285 58-59 0.02763560713564997 60-61 0.025598481750164315 62-63 0.010508221563252982 64-65 0.015741423433298242 66-67 0.02323876172885395 68-69 0.026590161556588415 70-71 0.029135074794761115 72-73 0.022372535391917237 74-75 0.03271348566252037 76-77 0.029589096874810702 78-79 0.02095670337913102 80-81 0.009420958161028966 82-83 0.0018160883201983552 84-85 0.007807984981905427 86-87 0.0272711946766628 88-89 0.024977188377464876 90-91 0.021195662368630803 92-93 0.04375339097741037 94-95 0.04546792172707132 96-97 0.034350354740593884 98-99 0.04132198325925007 100-101 0.041029258497112836 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8369637.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.71137786875477 #Duplication Level Percentage of deduplicated Percentage of total 1 89.33088164476099 31.00797988120308 2 4.9022763350970475 3.403295325692157 3 1.5186440294721018 1.581426802654034 4 0.8600103380595032 1.1940857526167576 5 0.5541056784764608 0.9616885792409581 6 0.4107027734176848 0.8553635495908096 7 0.2681894713483008 0.6516458256274713 8 0.18925067033999646 0.5255321224069405 9 0.15897541115808528 0.4966430011694055 >10 1.3752619435683884 9.609381764310951 >50 0.19882161005317692 4.756215912970396 >100 0.17628512115174547 13.582659019083623 >500 0.029276085673400735 7.043817963020424 >1k 0.026453878545063664 16.609152518008248 >5k 6.229637182477631E-4 1.3272613541890455 >10k+ 2.4204515939367063E-4 6.393850628215607 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 455514 5.4424582571502205 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 16479 0.19689025939834667 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 14189 0.16952945510062145 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 13505 0.16135705765972885 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 11502 0.13742531486132553 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 10848 0.1296113559046826 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10675 0.12754436064550947 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 9957 0.11896573292246725 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 9053 0.10816478659707704 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001947515764423236 0.0 0.0 2 0.0020908911581231063 0.0 0.0 3 0.0023059542486729115 0.0 0.0 4 0.0024373816928977923 0.0 0.0 5 0.0027121845308225433 0.0 0.0 6 0.003213998408772089 0.0 0.0 7 0.0036441245898716996 0.0 0.0 8 0.00421762616467118 0.0 0.0 9 0.004826971587895628 0.0 0.0 10-11 0.007885646653492857 0.0 0.0 12-13 0.010502247588515487 0.0 5.973974737494589E-6 14-15 0.013525078805687749 0.0 1.1947949474989179E-5 16-17 0.015872850877523124 0.0 1.1947949474989179E-5 18-19 0.019110745185245193 0.0 2.3895898949978357E-5 20-21 0.02350759059204121 0.0 2.3895898949978357E-5 22-23 0.028095603190437052 0.0 2.3895898949978357E-5 24-25 0.03371113944368197 0.0 2.3895898949978357E-5 26-27 0.039768749827501476 0.0 2.3895898949978357E-5 28-29 0.046274408316633084 0.0 2.3895898949978357E-5 30-31 0.0719923695615473 0.0 3.584384842496753E-5 32-33 0.07966295312449034 0.0 3.584384842496753E-5 34-35 0.0865389980473466 0.0 3.584384842496753E-5 36-37 0.09381529927761503 0.0 4.7791797899956715E-5 38-39 0.10159938836057048 0.0 4.7791797899956715E-5 40-41 0.1101839900583502 0.0 4.7791797899956715E-5 42-43 0.11870885200875497 0.0 4.7791797899956715E-5 44-45 0.12813578414452143 0.0 4.7791797899956715E-5 46-47 0.1380047904108625 0.0 5.3765772637451303E-5 48-49 0.14864443941834038 0.0 5.973974737494589E-5 50-51 0.15960668306164294 0.0 5.973974737494589E-5 52-53 0.17150086676399467 0.0 5.973974737494589E-5 54-55 0.1842851727022331 0.0 5.973974737494589E-5 56-57 0.19742194314998368 0.0 7.168769684993508E-5 58-59 0.21227324434739525 0.0 8.363564632492425E-5 60-61 0.2281998609975558 0.0 8.363564632492425E-5 62-63 0.2463069784269019 0.0 9.558359579991343E-5 64-65 0.2643424081593981 0.0 1.0753154527490261E-4 66-67 0.28513184024587923 0.0 1.0753154527490261E-4 68-69 0.30799424156627103 0.0 1.0753154527490261E-4 70-71 0.331800530895187 0.0 1.0753154527490261E-4 72-73 0.35852809387073775 0.0 1.0753154527490261E-4 74-75 0.3887325101435104 0.0 1.3142744422488096E-4 76-77 0.4213085943870684 0.0 1.3142744422488096E-4 78-79 0.458711650218522 0.0 1.3740141896237553E-4 80-81 0.5004458377346592 0.0 1.5532334317485931E-4 82-83 0.5477178998324539 0.0 1.5532334317485931E-4 84-85 0.5992195360443948 0.0 1.612973179123539E-4 86-87 0.6578779939918542 0.0 1.672712926498485E-4 88-89 0.7240815820327692 0.0 1.672712926498485E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 59430 0.0 81.53667 3 CGGAAGA 59220 0.0 81.48096 4 GATCGGA 60055 0.0 80.60778 1 GAAGAGC 60195 0.0 80.134636 6 ATCGGAA 60535 0.0 79.66387 2 GGAAGAG 63135 0.0 76.60612 5 GAGCACA 89430 0.0 54.071087 9 AGAGCAC 89085 0.0 54.051258 8 AAGAGCA 89925 0.0 53.541077 7 TTGTGTT 94945 0.0 46.421394 1 ATCGGAC 430 0.0 46.38864 2 GCCGTCT 52605 0.0 45.600998 50-51 ATGCCGT 52615 0.0 45.50014 48-49 CGTCTTC 52765 0.0 45.410194 52-53 TCGTATG 52985 0.0 45.389824 44-45 TGCCGTC 52635 0.0 45.28621 50-51 TCTCGTA 52525 0.0 45.284527 42-43 TATGCCG 52555 0.0 45.2222 48-49 CGTATCT 52335 0.0 45.19801 38-39 CGTATGC 52745 0.0 45.155792 46-47 >>END_MODULE