FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_34302-1.341000000358ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_34302-1.341000000358ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8369637
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT4555145.4424582571502205TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC164790.19689025939834667No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA141890.16952945510062145No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG135050.16135705765972885No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA115020.13742531486132553No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC108480.1296113559046826No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC106750.12754436064550947No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA99570.11896573292246725No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG90530.10816478659707704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG594300.081.536673
CGGAAGA592200.081.480964
GATCGGA600550.080.607781
GAAGAGC601950.080.1346366
ATCGGAA605350.079.663872
GGAAGAG631350.076.606125
GAGCACA894300.054.0710879
AGAGCAC890850.054.0512588
AAGAGCA899250.053.5410777
TTGTGTT949450.046.4213941
ATCGGAC4300.046.388642
GCCGTCT526050.045.60099850-51
ATGCCGT526150.045.5001448-49
CGTCTTC527650.045.41019452-53
TCGTATG529850.045.38982444-45
TGCCGTC526350.045.2862150-51
TCTCGTA525250.045.28452742-43
TATGCCG525550.045.222248-49
CGTATCT523350.045.1980138-39
CGTATGC527450.045.15579246-47
Produced by FastQC (version 0.11.2)