##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_34298-1.341000000357c1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5319493 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64153388302231 33.0 33.0 33.0 33.0 33.0 2 32.659842394754534 33.0 33.0 33.0 33.0 33.0 3 32.71384660154643 33.0 33.0 33.0 33.0 33.0 4 36.65857253689402 37.0 37.0 37.0 37.0 37.0 5 36.613861884957835 37.0 37.0 37.0 37.0 37.0 6 36.51670206164385 37.0 37.0 37.0 37.0 37.0 7 36.463688362781944 37.0 37.0 37.0 37.0 37.0 8 36.40012215449856 37.0 37.0 37.0 37.0 37.0 9 36.41002215812672 37.0 37.0 37.0 37.0 37.0 10-11 36.364083663612305 37.0 37.0 37.0 37.0 37.0 12-13 36.34656310291225 37.0 37.0 37.0 37.0 37.0 14-15 38.16862772448427 40.0 37.0 40.0 37.0 40.0 16-17 38.15068419114378 40.0 37.0 40.0 37.0 40.0 18-19 38.13988974137197 40.0 37.0 40.0 37.0 40.0 20-21 38.09052648438488 40.0 37.0 40.0 37.0 40.0 22-23 37.98484489029312 40.0 37.0 40.0 33.0 40.0 24-25 37.93607858869257 40.0 37.0 40.0 33.0 40.0 26-27 37.8293390930301 40.0 37.0 40.0 33.0 40.0 28-29 37.7070176612696 40.0 37.0 40.0 33.0 40.0 30-31 37.655192891503006 40.0 37.0 40.0 33.0 40.0 32-33 37.52044076380963 40.0 37.0 40.0 33.0 40.0 34-35 37.441029812427615 40.0 37.0 40.0 33.0 40.0 36-37 37.37100067619226 40.0 37.0 40.0 33.0 40.0 38-39 37.24510850940118 40.0 37.0 40.0 33.0 40.0 40-41 37.1339721661444 40.0 37.0 40.0 33.0 40.0 42-43 36.98134803448374 38.5 37.0 40.0 33.0 40.0 44-45 36.87623923934105 37.0 37.0 40.0 33.0 40.0 46-47 36.82227046825703 37.0 37.0 40.0 33.0 40.0 48-49 36.56961368310852 37.0 37.0 40.0 33.0 40.0 50-51 36.40924830618256 37.0 37.0 40.0 33.0 40.0 52-53 36.29129843765186 37.0 37.0 40.0 33.0 40.0 54-55 36.02219234051065 37.0 37.0 40.0 33.0 40.0 56-57 35.882802082830075 37.0 37.0 40.0 30.0 40.0 58-59 35.60746418878641 37.0 35.0 40.0 27.0 40.0 60-61 35.339620711973865 37.0 33.0 40.0 27.0 40.0 62-63 35.03461937068063 37.0 33.0 38.5 27.0 40.0 64-65 34.76612423402005 37.0 33.0 37.0 27.0 40.0 66-67 34.3740951440297 37.0 33.0 37.0 27.0 40.0 68-69 34.09535720791436 37.0 33.0 37.0 27.0 40.0 70-71 33.641195974879565 37.0 33.0 37.0 27.0 38.5 72-73 33.27395608942431 37.0 33.0 37.0 27.0 37.0 74-75 32.792460672473865 37.0 33.0 37.0 22.0 37.0 76-77 30.233836946490953 33.0 30.0 35.0 22.0 37.0 78-79 32.47180624168506 33.0 33.0 37.0 24.5 37.0 80-81 32.851774219836365 37.0 33.0 37.0 27.0 37.0 82-83 32.84758791862307 37.0 33.0 37.0 27.0 37.0 84-85 32.71602895238324 37.0 33.0 37.0 27.0 37.0 86-87 32.591461911877694 37.0 33.0 37.0 22.0 37.0 88-89 32.39130684070832 35.0 33.0 37.0 22.0 37.0 90-91 32.128818103529795 33.0 33.0 37.0 22.0 37.0 92-93 32.07678692311467 33.0 33.0 37.0 22.0 37.0 94-95 32.00947580906677 33.0 33.0 37.0 22.0 37.0 96-97 31.842423516677247 33.0 33.0 37.0 22.0 37.0 98-99 31.676487496082803 33.0 33.0 37.0 18.5 37.0 100-101 30.105221117877214 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.16748559885844827 2306 2 0.16991084942752366 2306 3 0.14013492326350985 2306 4 0.13455178103781407 2306 5 0.1790933890900419 2306 6 0.2099835140061117 2306 7 0.22719176062578583 2306 8 0.19317576678281512 2306 9 0.22196660163933046 2306 10-11 0.23337452145895554 2306 12-13 0.24956327993061933 2306 14-15 0.4892819705567675 2306 16-17 0.4574557923453426 2306 18-19 0.4091388637382565 2306 20-21 0.34949847437192716 2306 22-23 0.4902320927695385 2306 24-25 0.44381016384087957 2306 26-27 0.5008028301097269 2306 28-29 0.5932696201799672 2306 30-31 0.5282875243329812 2306 32-33 0.4453388491042105 2306 34-35 0.3694797322254857 2306 36-37 0.3930748365248036 2306 38-39 0.443510245115462 2306 40-41 0.43371554534057566 2306 42-43 0.3559083962223326 2306 44-45 0.37103004252528393 2306 46-47 0.4367730902427027 2306 48-49 0.4985079640373016 2306 50-51 0.387468980336358 2306 52-53 0.3871074615453196 2306 54-55 0.4393780432527734 2306 56-57 0.5061023471078983 2306 58-59 0.5308147251547197 2306 60-61 0.5160210197494663 2306 62-63 0.37521148681369 2306 64-65 0.4041807638338426 2306 66-67 0.4806068680578477 2306 68-69 0.5233329981408517 2306 70-71 0.5135559694810965 2306 72-73 0.32348609106412596 2306 74-75 0.22309334666477554 2306 76-77 -0.02218981931639874 2306 78-79 0.47198339485735374 2306 80-81 0.45558566241915344 2306 82-83 0.41326251595976515 2306 84-85 0.5521346237314617 2306 86-87 0.4854256971793305 2306 88-89 0.6105641969458304 2306 90-91 0.6316818428624273 2306 92-93 0.5748194791369698 2306 94-95 0.438514770258017 2306 96-97 0.48480110217536776 2306 98-99 0.47431236284603884 2306 100-101 0.35216239816728034 2307 1 0.182894873675707 2307 2 0.15524164586633304 2307 3 0.11998741889663478 2307 4 0.1347841848421396 2307 5 0.20707706624605748 2307 6 0.16739302286345037 2307 7 0.13688000118857957 2307 8 0.167942403646407 2307 9 0.1771713093813858 2307 10-11 0.1960031399157245 2307 12-13 0.18765300194494472 2307 14-15 0.4023427846098073 2307 16-17 0.3739027979176015 2307 18-19 0.35203049806166575 2307 20-21 0.31537823889403427 2307 22-23 0.36628865783919196 2307 24-25 0.42562853807730505 2307 26-27 0.4347988564955614 2307 28-29 0.5149848763786409 2307 30-31 0.35296363561296573 2307 32-33 0.3827915323916642 2307 34-35 0.4725565991899572 2307 36-37 0.4348186610257869 2307 38-39 0.38647118359401134 2307 40-41 0.4178525737483696 2307 42-43 0.4376905454460527 2307 44-45 0.3339349538990959 2307 46-47 0.48359942178903736 2307 48-49 0.5571073455359894 2307 50-51 0.4247366167791071 2307 52-53 0.23917315027211572 2307 54-55 0.4431421614671791 2307 56-57 0.45948743810035353 2307 58-59 0.47752225787242963 2307 60-61 0.41165096786424016 2307 62-63 0.3763541080615269 2307 64-65 0.290863561354918 2307 66-67 0.24817260790504037 2307 68-69 0.35209750035201637 2307 70-71 0.3317722789920836 2307 72-73 0.27507851105808356 2307 74-75 0.23977115671470273 2307 76-77 -0.05914743446805204 2307 78-79 0.23845122070683544 2307 80-81 0.42075253896005194 2307 82-83 0.35413042144832474 2307 84-85 0.3617632880567001 2307 86-87 0.2912783934936414 2307 88-89 0.5050009312516934 2307 90-91 0.5494262823916003 2307 92-93 0.402606096024293 2307 94-95 0.40606042087333805 2307 96-97 0.29068267082239885 2307 98-99 0.26823057222513214 2307 100-101 0.18629047720758507 2201 1 0.11284676372507363 2201 2 0.16032289703156266 2201 3 0.11900707716377212 2201 4 0.13373723383338643 2201 5 0.16238992971732813 2201 6 0.12120214349354086 2201 7 0.17402461677097847 2201 8 0.15279496825480265 2201 9 0.15895959931197012 2201 10-11 0.1678450232828297 2201 12-13 0.1366887471209779 2201 14-15 0.3140240429071426 2201 16-17 0.3939653084663419 2201 18-19 0.2980320245697001 2201 20-21 0.35023187728561567 2201 22-23 0.42221488213925795 2201 24-25 0.4222884027945071 2201 26-27 0.405403632514556 2201 28-29 0.4620150276650108 2201 30-31 0.4213460920886689 2201 32-33 0.4479496138600183 2201 34-35 0.48737785743867335 2201 36-37 0.5286910936982139 2201 38-39 0.4328539056140528 2201 40-41 0.48508578462742946 2201 42-43 0.38166787904846444 2201 44-45 0.4598971119940032 2201 46-47 0.5544088514603729 2201 48-49 0.6011614459568833 2201 50-51 0.44350126319420013 2201 52-53 0.5320012159590775 2201 54-55 0.5759414418291016 2201 56-57 0.6159416661452113 2201 58-59 0.4915494125349582 2201 60-61 0.5253806027477026 2201 62-63 0.4660248742323674 2201 64-65 0.4380638613992005 2201 66-67 0.46884229448979653 2201 68-69 0.46638433695549253 2201 70-71 0.5269231237073129 2201 72-73 0.33863633319167263 2201 74-75 0.3841687586418985 2201 76-77 0.2129654192027779 2201 78-79 0.592286637865655 2201 80-81 0.5514670184229473 2201 82-83 0.5182754417949056 2201 84-85 0.6063506892221611 2201 86-87 0.6360521468797202 2201 88-89 0.658715127463978 2201 90-91 0.7502866282331127 2201 92-93 0.606223312007522 2201 94-95 0.5041773969788608 2201 96-97 0.5477997330785627 2201 98-99 0.42255352662971646 2201 100-101 0.5326181659792937 1101 1 0.10457358940794848 1101 2 0.11743619239094727 1101 3 0.09079797715015303 1101 4 0.11062182766422524 1101 5 0.14668612372057765 1101 6 0.11373565195424362 1101 7 0.13304860982962907 1101 8 0.17076450799936538 1101 9 0.11124676694321778 1101 10-11 0.175091879041112 1101 12-13 0.1548722506948863 1101 14-15 0.28775871135227504 1101 16-17 0.2791872727944522 1101 18-19 0.3222245156088377 1101 20-21 0.3272344805239342 1101 22-23 0.26485368499183437 1101 24-25 0.28852784493697214 1101 26-27 0.28061403965521237 1101 28-29 0.3137834460975526 1101 30-31 0.363394871341697 1101 32-33 0.26436393727234986 1101 34-35 0.278703919892493 1101 36-37 0.41220256615795847 1101 38-39 0.34816006561138124 1101 40-41 0.4153993193337939 1101 42-43 0.3997235058940518 1101 44-45 0.478250938182434 1101 46-47 0.46698340422582163 1101 48-49 0.5545449409056786 1101 50-51 0.4232276454335917 1101 52-53 0.4221840285861589 1101 54-55 0.5411299322672889 1101 56-57 0.4191913663169373 1101 58-59 0.4810297234797858 1101 60-61 0.4674111942549146 1101 62-63 0.37232892037298626 1101 64-65 0.4200999936062999 1101 66-67 0.33461204278405177 1101 68-69 0.3754083886086974 1101 70-71 0.40987694019248266 1101 72-73 0.3645099016391242 1101 74-75 0.40700841889668027 1101 76-77 0.16308775288744215 1101 78-79 0.4843154363798732 1101 80-81 0.5390698899305093 1101 82-83 0.46305861738001397 1101 84-85 0.3680131710666359 1101 86-87 0.3945546016394559 1101 88-89 0.4730705792993817 1101 90-91 0.5378282332876196 1101 92-93 0.3780305481521964 1101 94-95 0.44230903735181215 1101 96-97 0.4571034213237226 1101 98-99 0.42306029910406906 1101 100-101 0.4692325592831992 2304 1 0.13561210043854288 2304 2 0.13148269558972459 2304 3 0.09343983891269403 2304 4 0.09456571147685366 2304 5 0.15556021542374765 2304 6 0.07177492564547094 2304 7 0.1171615937604571 2304 8 0.12311754036380052 2304 9 0.14816473850579115 2304 10-11 0.10471678059926148 2304 12-13 0.1406561365685164 2304 14-15 0.3442092123674527 2304 16-17 0.2675233844867009 2304 18-19 0.26844943431240864 2304 20-21 0.25964902322293426 2304 22-23 0.3272868966636935 2304 24-25 0.2970333765585167 2304 26-27 0.35787058456713083 2304 28-29 0.447328765328983 2304 30-31 0.3129868456821896 2304 32-33 0.39686180852973507 2304 34-35 0.43875564933763656 2304 36-37 0.3863056403865599 2304 38-39 0.46136797525052486 2304 40-41 0.4250743379561399 2304 42-43 0.4748104656409424 2304 44-45 0.48988191259798697 2304 46-47 0.5140422525771626 2304 48-49 0.4954773412789777 2304 50-51 0.47529049364458587 2304 52-53 0.47780162157234685 2304 54-55 0.548390021752212 2304 56-57 0.5380696377734893 2304 58-59 0.5021784094337178 2304 60-61 0.5091818772603816 2304 62-63 0.4711011344740754 2304 64-65 0.3766353556080375 2304 66-67 0.36316340067087793 2304 68-69 0.4210864303342703 2304 70-71 0.4607334787081854 2304 72-73 0.28847585685934973 2304 74-75 0.3338950933898346 2304 76-77 0.27689142174259374 2304 78-79 0.5132852804142232 2304 80-81 0.5745369185167348 2304 82-83 0.5470543816596916 2304 84-85 0.6505234127949606 2304 86-87 0.48776958709915164 2304 88-89 0.6772682776366779 2304 90-91 0.7281770894521316 2304 92-93 0.42666406639852994 2304 94-95 0.4197765021248401 2304 96-97 0.5235259433096218 2304 98-99 0.5523196388096387 2304 100-101 0.49280090491371453 2202 1 0.12531109677543384 2202 2 0.1044349195744303 2202 3 0.0761428857868296 2202 4 0.057355072425608 2202 5 0.10794853984145192 2202 6 0.10849175165020597 2202 7 0.1072003842836935 2202 8 0.14208885316023157 2202 9 0.11540238643138423 2202 10-11 0.11190882623965592 2202 12-13 0.09175475151025836 2202 14-15 0.13380555592795673 2202 16-17 0.17125723036939888 2202 18-19 0.13543827032678735 2202 20-21 0.2019372036250644 2202 22-23 0.1891653233848558 2202 24-25 0.16560249371288194 2202 26-27 0.1611630949634062 2202 28-29 0.13997182423882037 2202 30-31 0.1842449415962477 2202 32-33 0.2762856827233193 2202 34-35 0.3303597160706744 2202 36-37 0.28872834117872515 2202 38-39 0.2850191590521902 2202 40-41 0.30892228482121453 2202 42-43 0.3985532668470455 2202 44-45 0.2735024831664177 2202 46-47 0.3896882981826266 2202 48-49 0.4201645003931631 2202 50-51 0.39241167473106486 2202 52-53 0.33718478397650387 2202 54-55 0.37235083109750633 2202 56-57 0.41515544215683775 2202 58-59 0.3480845246175974 2202 60-61 0.41212816278594033 2202 62-63 0.3126161243199519 2202 64-65 0.3656190375479724 2202 66-67 0.3590001279765218 2202 68-69 0.22368664977145158 2202 70-71 0.39391311074600566 2202 72-73 0.2439211380866695 2202 74-75 0.2898127520491087 2202 76-77 0.06659295859676817 2202 78-79 0.4254322080555255 2202 80-81 0.45589992191626294 2202 82-83 0.3731059573361435 2202 84-85 0.3663435314688783 2202 86-87 0.3778255261140373 2202 88-89 0.4146764419065221 2202 90-91 0.580409489621708 2202 92-93 0.4849518688987118 2202 94-95 0.5367121460586404 2202 96-97 0.3182501622196483 2202 98-99 0.16909372172259296 2202 100-101 0.29371168035898165 1102 1 0.09576693349585952 1102 2 0.1147821240657052 1102 3 0.08026106550692447 1102 4 0.04182698162477294 1102 5 0.11768158273231677 1102 6 0.0672210121950414 1102 7 0.07912223471196 1102 8 0.06733288847030394 1102 9 0.0854066111884606 1102 10-11 0.036718501316734375 1102 12-13 0.05872075486706052 1102 14-15 0.18175386514337077 1102 16-17 0.10531306616972103 1102 18-19 0.10416740052521334 1102 20-21 0.1311223008903184 1102 22-23 0.17991264534124696 1102 24-25 0.16831638258260284 1102 26-27 0.1627606051936752 1102 28-29 0.1256172344734665 1102 30-31 0.10798155904564055 1102 32-33 0.1665826961804342 1102 34-35 0.21566982934169943 1102 36-37 0.16857578987783484 1102 38-39 0.1321669113050774 1102 40-41 0.20492847090834942 1102 42-43 0.2702038433998766 1102 44-45 0.3234611940251213 1102 46-47 0.3045153553364628 1102 48-49 0.14340074098809197 1102 50-51 0.09983766289101226 1102 52-53 0.12432181715426083 1102 54-55 0.19582690908146105 1102 56-57 0.25111793919528935 1102 58-59 0.23257767261367945 1102 60-61 0.07914944835421522 1102 62-63 0.1933246260493391 1102 64-65 0.15971465200477297 1102 66-67 0.158660383900191 1102 68-69 0.0929281180240551 1102 70-71 0.13997645756636246 1102 72-73 0.14651863528230535 1102 74-75 0.1842356126620288 1102 76-77 -0.04751266953106992 1102 78-79 0.3675415349543556 1102 80-81 0.31692841640759184 1102 82-83 0.25475065296578236 1102 84-85 0.24331530840477456 1102 86-87 0.1541576088886103 1102 88-89 0.3217053335710247 1102 90-91 0.37562224946152156 1102 92-93 0.320756033389749 1102 94-95 0.20285934395592875 1102 96-97 0.021863134600778267 1102 98-99 0.012617077115134379 1102 100-101 0.009395113407315137 2305 1 0.12256596373367756 2305 2 0.12155579289574092 2305 3 0.0740353435742449 2305 4 0.013360682944885127 2305 5 0.10471928269077324 2305 6 0.07684198246283103 2305 7 0.1126651762917561 2305 8 0.10367056378523642 2305 9 0.0017603578626932403 2305 10-11 0.09863121307107292 2305 12-13 0.041417142226954695 2305 14-15 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quality scores pass #Quality Count 7 14.0 8 102.0 9 304.0 10 259.0 11 118.0 12 148.0 13 322.0 14 943.0 15 2351.0 16 4812.0 17 7149.0 18 9105.0 19 10775.0 20 13166.0 21 15946.0 22 20009.0 23 24808.0 24 30912.0 25 39009.0 26 49534.0 27 65637.0 28 92610.0 29 123782.0 30 119620.0 31 129033.0 32 168550.0 33 237736.0 34 361634.0 35 621657.0 36 1194177.0 37 1655041.0 38 320191.0 39 39.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.47992786030392 24.966956689438835 24.32428360332358 14.228831846933668 2 30.90322705566113 23.12684686303751 32.76885597931983 13.201070101981523 3 36.83330347459805 18.01846529359095 28.660118548891784 16.48811268291922 4 29.910191984866362 17.64317928908991 33.887452427734246 18.55917629830948 5 31.341802686835006 16.675367370537003 35.60281026782064 16.380019674807357 6 26.791651008846145 17.481515625643272 39.505325037555274 16.221508327955313 7 33.11214057429909 20.044429046151578 29.325181929932043 17.518248449617285 8 29.74546634425499 28.505254166139515 24.54735817868357 17.201921310921925 9 30.37058230925391 25.216745280048304 25.416106384574622 18.996566026123165 10-11 24.927966323939206 27.264175364567382 29.479270825754924 18.32858748573849 12-13 24.02121781154708 26.153075114489294 28.809230503734096 21.016476570229532 14-15 23.24567397682448 26.853621200366273 29.612615337589503 20.288089485219736 16-17 23.14691334259239 26.832229339882353 30.611060187131212 19.409797130394047 18-19 20.9551831349341 27.095871730632975 31.804299770673634 20.144645363759288 20-21 22.363390646439427 27.31488696385163 31.12720516786092 19.194517221848024 22-23 21.094613716006393 29.743520670108975 30.14033480258363 19.021530811301 24-25 21.583889667680737 26.730254180238607 31.47988915484991 20.20596699723075 26-27 21.266528595864305 26.77419633788408 30.294851407831537 21.66442365842008 28-29 21.94864247401021 28.34597206914268 29.81849962017057 19.88688583667654 30-31 20.19223448550454 27.83294385385976 30.69886547458564 21.275956186050063 32-33 19.899283634737372 29.246527817594647 30.21349966998735 20.640688877680635 34-35 21.470760603572614 28.082378159194697 30.364973726347017 20.08188751088567 36-37 19.926476075821512 28.89244332119621 31.68781310549708 19.493267497485192 38-39 20.468138144178727 29.82637041604551 29.549570719840883 20.15592071993488 40-41 20.523475265138075 27.888412884547144 32.1070657269824 19.481046123332384 42-43 20.36544478572443 29.34755135187011 31.37100213399993 18.91600172840553 44-45 20.037891406042625 28.49149492462701 31.503763949850093 19.966849719480273 46-47 21.62447084851674 28.035915278610464 30.341414919623116 19.998198953249677 48-49 20.89905291607696 30.1250558368916 30.146732583025887 18.829158664005547 50-51 21.52994145544901 29.28658216644354 29.77719193329369 19.406284444813757 52-53 19.951646273313237 28.794559641746257 29.204927116909047 22.048866968031454 54-55 21.696694000593773 28.671668161750315 30.08337057724268 19.548267260413237 56-57 20.585264190657895 28.454881364820462 29.540981852665976 21.418872591855667 58-59 20.378776682021794 28.307717012899737 30.279823936921762 21.03368236815671 60-61 21.162134851447245 28.417147752482148 30.45896545771879 19.961751938351817 62-63 20.190851721374877 28.38517255110317 30.00883069690875 21.415145030613207 64-65 21.589465209613962 28.219829649525952 29.839836260186264 20.35086888067382 66-67 20.967813659881504 30.767333111269746 28.236428713592783 20.028424515255967 68-69 21.468108012768774 30.712973290143967 28.072957216826477 19.745961480260778 70-71 20.78359589086818 31.51977554005644 28.246276002917632 19.450352566157747 72-73 20.288830725376716 31.66230440325365 28.31109895192861 19.737765919441028 74-75 21.359544076289165 30.76060592128033 27.842718241941082 20.037131760489427 76-77 21.76878129243859 29.48428895343305 28.47343482804931 20.273494926079046 78-79 21.57105132455234 29.40199621334872 29.131098064001907 19.89585439809703 80-81 20.54973022230269 30.05310763715572 29.06302659010353 20.334135550438063 82-83 19.61192825585879 29.82511383326287 29.229868464562657 21.333089446315686 84-85 19.43294130431227 29.256173860173913 29.908711639740638 21.402173195773177 86-87 19.691519469645076 29.292525393002272 30.53382421049326 20.48213092685939 88-89 19.839411815972678 29.91359199668986 30.024813849123184 20.222182338214278 90-91 20.225435606795973 29.526976507754334 29.38059829672922 20.86698958872047 92-93 19.57479940307546 30.340974807489957 29.261047288497544 20.823178500937043 94-95 19.456036374325603 30.699328607374206 29.36204441584102 20.482590602459172 96-97 20.17728618952882 31.069350180373036 28.5387118694172 20.214651760680944 98-99 20.461421095505088 30.308336540598045 28.302113710471303 20.92812865342556 100-101 20.23973238738008 30.301035988067447 28.36629829968194 21.09293332487053 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4529.0 1 2740.5 2 1056.0 3 1699.5 4 1825.5 5 1217.5 6 1084.5 7 1189.0 8 1244.0 9 1241.0 10 1167.5 11 1055.5 12 967.0 13 927.0 14 923.0 15 965.5 16 1028.5 17 1123.5 18 1231.0 19 1344.0 20 1568.5 21 1887.5 22 2350.5 23 2997.0 24 3798.5 25 4878.0 26 6353.0 27 8324.5 28 10591.5 29 13085.0 30 16437.0 31 21694.0 32 33337.5 33 54456.0 34 83890.0 35 125544.0 36 180420.0 37 270552.0 38 379379.5 39 439332.5 40 437406.5 41 383670.0 42 335541.5 43 328685.0 44 320127.0 45 308164.5 46 304643.5 47 271604.0 48 212293.5 49 169193.5 50 140176.0 51 115884.0 52 93106.0 53 66986.0 54 44204.0 55 30392.5 56 20777.0 57 14392.5 58 10193.0 59 7030.0 60 4994.0 61 3794.0 62 2722.0 63 1791.0 64 1256.0 65 863.0 66 643.0 67 502.5 68 347.5 69 243.5 70 188.5 71 148.0 72 96.0 73 64.5 74 53.0 75 35.5 76 27.0 77 23.5 78 16.5 79 12.0 80 9.0 81 6.5 82 3.0 83 2.5 84 2.0 85 2.5 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1841340894705567 2 0.0 3 0.0 4 0.009023416329338153 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0019926711060621756 12-13 0.0 14-15 0.0 16-17 3.195793283307263E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 9.399392009727243E-6 36-37 0.0 38-39 0.0023122504343929015 40-41 0.006212998118429708 42-43 0.002622430370713901 44-45 0.0015227015055758134 46-47 0.0068427573830814326 48-49 0.008064678344345974 50-51 0.0055644400697585274 52-53 0.006729964678964706 54-55 0.01785884481848176 56-57 0.0208384520855653 58-59 0.026299498843216827 60-61 0.02472040098558265 62-63 0.010282934858641605 64-65 0.015245813839777588 66-67 0.02249274507927729 68-69 0.025613343226506734 70-71 0.027897395484870458 72-73 0.021674997974431023 74-75 0.03145976505655708 76-77 0.028480157789473548 78-79 0.02018049464488439 80-81 0.009098611465415971 82-83 0.0016918905617509038 84-85 0.007782696584054158 86-87 0.026205504923119554 88-89 0.024316227129164376 90-91 0.020274488564981662 92-93 0.04207167863553914 94-95 0.04391395946944568 96-97 0.03315165561830798 98-99 0.04018240084158396 100-101 0.03958083975296142 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5319493.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.33709426274321 #Duplication Level Percentage of deduplicated Percentage of total 1 85.43692258374723 28.482187416930753 2 6.47760476231378 4.318890411160975 3 2.2017994415855684 2.2020478659538036 4 1.2187189939654448 1.625141999264864 5 0.780706397752266 1.3013241386696992 6 0.5545325960080364 1.1091903254930167 7 0.40592020924105565 0.9472540195035063 8 0.3211063336154776 0.8563801689682434 9 0.23467662615577387 0.7041093126675824 >10 1.8177551367079394 11.992502830847233 >50 0.2519816247476526 5.898438396107302 >100 0.23559302999708454 17.13821423410004 >500 0.04433383402873028 10.215112307835621 >1k 0.017895746873725627 9.748292412134338 >5k 2.2634280085410234E-4 0.5494324357037338 >10k+ 2.2634045940689545E-4 2.9114817246594336 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 113861 2.1404483472391074 TruSeq Adapter, Index 2 (97% over 37bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15220 0.28611749277609727 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 13091 0.24609488159867865 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 12170 0.2287812015167611 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 8958 0.16839950724627328 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 8303 0.15608630371353058 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 6347 0.11931588217147762 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT 5518 0.10373169021934985 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0014475063694979954 0.0 0.0 2 0.0015415002895952678 0.0 0.0 3 0.0017294881297898127 0.0 0.0 4 0.0018798784019454485 0.0 0.0 5 0.0022746528663539927 0.0 0.0 6 0.0025002382745874466 0.0 0.0 7 0.0029138115230154453 0.0 0.0 8 0.003402579907521262 0.0 0.0 9 0.0039665434281048965 0.0 0.0 10-11 0.006617171974847978 0.0 0.0 12-13 0.009709571946048242 0.0 0.0 14-15 0.01391110017439632 0.0 0.0 16-17 0.01796223813058876 0.0 0.0 18-19 0.023639470904464013 0.0 0.0 20-21 0.028658746237658364 0.0 0.0 22-23 0.03450516806770871 0.0 0.0 24-25 0.040746364362167595 0.0 0.0 26-27 0.04852906094622175 0.0 0.0 28-29 0.05755247727555991 0.0 0.0 30-31 0.08712296453816182 0.0 0.0 32-33 0.09861842096605823 0.0 0.0 34-35 0.10855357832033993 0.0 0.0 36-37 0.11926888521142898 0.0 0.0 38-39 0.1304541617030044 0.0 0.0 40-41 0.142081209619037 0.0 0.0 42-43 0.15520276086461623 0.0 0.0 44-45 0.168822479886711 0.0 0.0 46-47 0.18430327852673178 0.0 0.0 48-49 0.20099659873600734 0.0 1.8798784019454487E-5 50-51 0.2182256842898374 0.0 3.759756803890897E-5 52-53 0.23611272728434834 0.0 3.759756803890897E-5 54-55 0.25499610583189036 0.0 3.759756803890897E-5 56-57 0.27475362783633706 0.0 3.759756803890897E-5 58-59 0.2954886866097953 0.0 3.759756803890897E-5 60-61 0.3174080687764793 0.0 3.759756803890897E-5 62-63 0.34069036278457365 0.0 3.759756803890897E-5 64-65 0.3649595929536894 0.0 3.759756803890897E-5 66-67 0.39147527781312996 0.0 3.759756803890897E-5 68-69 0.41970165201834086 0.0 3.759756803890897E-5 70-71 0.4480314195356587 0.0 3.759756803890897E-5 72-73 0.4800645475048092 0.0 3.759756803890897E-5 74-75 0.5119002882417554 0.0 3.759756803890897E-5 76-77 0.5454373189324622 0.0 3.759756803890897E-5 78-79 0.5835330547478867 0.0 3.759756803890897E-5 80-81 0.623320681125062 0.0 3.759756803890897E-5 82-83 0.6665954819378463 0.0 3.759756803890897E-5 84-85 0.7143161951712316 0.0 3.759756803890897E-5 86-87 0.7651011101997878 0.0 3.759756803890897E-5 88-89 0.8195423887201281 0.0 3.759756803890897E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGCG 35 3.382389E-6 67.97936 1 CGGAAGA 18145 0.0 67.05748 4 TCGGAAG 18245 0.0 66.872154 3 GATCGGA 18600 0.0 65.57084 1 GAAGAGC 18720 0.0 64.92043 6 ATCGGAA 18885 0.0 64.27898 2 GGAAGAG 19725 0.0 61.78123 5 CGGCGAG 100 1.2732926E-11 47.58555 1 CGCGTGG 50 2.7984692E-5 47.58555 1 GTGTCGG 115 1.8189894E-12 45.516613 1 GCCGTCT 14045 0.0 43.00326 50-51 CGTCTTC 14090 0.0 42.952625 52-53 ATGCCGT 14180 0.0 42.391064 48-49 TGCCGTC 14160 0.0 42.38564 50-51 TCGTATG 14325 0.0 42.307156 44-45 TATGCCG 14165 0.0 42.184456 48-49 CGTATGC 14250 0.0 42.147316 46-47 CTGCTTG 14450 0.0 41.984306 58-59 CTCGTAT 14220 0.0 41.96823 44-45 TCTCGTA 14295 0.0 41.9294 42-43 >>END_MODULE