FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n01_34298-1.341000000357c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n01_34298-1.341000000357c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5319493
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1138612.1404483472391074TruSeq Adapter, Index 2 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152200.28611749277609727No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC130910.24609488159867865No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG121700.2287812015167611No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA89580.16839950724627328No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA83030.15608630371353058No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA63470.11931588217147762No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT55180.10373169021934985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCG353.382389E-667.979361
CGGAAGA181450.067.057484
TCGGAAG182450.066.8721543
GATCGGA186000.065.570841
GAAGAGC187200.064.920436
ATCGGAA188850.064.278982
GGAAGAG197250.061.781235
CGGCGAG1001.2732926E-1147.585551
CGCGTGG502.7984692E-547.585551
GTGTCGG1151.8189894E-1245.5166131
GCCGTCT140450.043.0032650-51
CGTCTTC140900.042.95262552-53
ATGCCGT141800.042.39106448-49
TGCCGTC141600.042.3856450-51
TCGTATG143250.042.30715644-45
TATGCCG141650.042.18445648-49
CGTATGC142500.042.14731646-47
CTGCTTG144500.041.98430658-59
CTCGTAT142200.041.9682344-45
TCTCGTA142950.041.929442-43
Produced by FastQC (version 0.11.2)