##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n01_34274-2.3410000003583d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4617420 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66738611605615 33.0 33.0 33.0 33.0 33.0 2 32.68947918967735 33.0 33.0 33.0 33.0 33.0 3 32.73825859462644 33.0 33.0 33.0 33.0 33.0 4 36.691827903894385 37.0 37.0 37.0 37.0 37.0 5 36.653439583143836 37.0 37.0 37.0 37.0 37.0 6 36.554614698251406 37.0 37.0 37.0 37.0 37.0 7 36.51752926959211 37.0 37.0 37.0 37.0 37.0 8 36.468803790861564 37.0 37.0 37.0 37.0 37.0 9 36.468323652602535 37.0 37.0 37.0 37.0 37.0 10-11 36.437329179498505 37.0 37.0 37.0 37.0 37.0 12-13 36.423146584023115 37.0 37.0 37.0 37.0 37.0 14-15 38.33603668282288 40.0 37.0 40.0 37.0 40.0 16-17 38.31986726353678 40.0 37.0 40.0 37.0 40.0 18-19 38.30635733808057 40.0 37.0 40.0 37.0 40.0 20-21 38.240030904704355 40.0 37.0 40.0 37.0 40.0 22-23 38.14936382222106 40.0 37.0 40.0 37.0 40.0 24-25 38.109037514456126 40.0 37.0 40.0 37.0 40.0 26-27 38.035506841482906 40.0 37.0 40.0 37.0 40.0 28-29 37.91904884112773 40.0 37.0 40.0 33.0 40.0 30-31 37.879137591988595 40.0 37.0 40.0 33.0 40.0 32-33 37.76282664345024 40.0 37.0 40.0 33.0 40.0 34-35 37.67074762529724 40.0 37.0 40.0 33.0 40.0 36-37 37.59247144509271 40.0 37.0 40.0 33.0 40.0 38-39 37.52096138103097 40.0 37.0 40.0 33.0 40.0 40-41 37.43879417510212 40.0 37.0 40.0 33.0 40.0 42-43 37.30232402510493 40.0 37.0 40.0 33.0 40.0 44-45 37.19169005635182 40.0 37.0 40.0 33.0 40.0 46-47 37.139151625799684 40.0 37.0 40.0 33.0 40.0 48-49 36.94431294965587 38.5 37.0 40.0 33.0 40.0 50-51 36.81222598334135 37.0 37.0 40.0 33.0 40.0 52-53 36.68535599967082 37.0 37.0 40.0 33.0 40.0 54-55 36.44434965846728 37.0 37.0 40.0 33.0 40.0 56-57 36.310381879924286 37.0 37.0 40.0 33.0 40.0 58-59 36.11961679899164 37.0 37.0 40.0 33.0 40.0 60-61 35.85248861918561 37.0 37.0 40.0 30.0 40.0 62-63 35.553184029176464 37.0 35.0 40.0 27.0 40.0 64-65 35.32838847235036 37.0 33.0 38.5 27.0 40.0 66-67 35.02482945021246 37.0 33.0 37.0 27.0 40.0 68-69 34.765418133936265 37.0 33.0 37.0 27.0 40.0 70-71 34.38285449450126 37.0 33.0 37.0 27.0 40.0 72-73 34.135574411684445 37.0 33.0 37.0 27.0 40.0 74-75 33.76257184748193 37.0 33.0 37.0 27.0 37.0 76-77 31.149284448891372 33.0 30.0 35.0 24.5 37.0 78-79 33.40059459611645 35.0 33.0 37.0 27.0 37.0 80-81 33.75247291777659 37.0 33.0 37.0 27.0 37.0 82-83 33.72244662603792 37.0 33.0 37.0 27.0 37.0 84-85 33.575831308393 37.0 33.0 37.0 27.0 37.0 86-87 33.42995590611207 37.0 33.0 37.0 27.0 37.0 88-89 33.21148682597641 37.0 33.0 37.0 27.0 37.0 90-91 32.9567055845039 37.0 33.0 37.0 27.0 37.0 92-93 32.943954524388076 33.0 33.0 37.0 27.0 37.0 94-95 32.87068341194866 33.0 33.0 37.0 27.0 37.0 96-97 32.708986945090544 33.0 33.0 37.0 27.0 37.0 98-99 32.55445281131021 33.0 33.0 37.0 27.0 37.0 100-101 30.96948382430015 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.2050368211319693 2306 2 0.1570122905646727 2306 3 0.10752110517982061 2306 4 0.13027766135891028 2306 5 0.21501490038563986 2306 6 0.16066624858164147 2306 7 0.10633498441222144 2306 8 0.1490506666320428 2306 9 0.2036826402304115 2306 10-11 0.14120879964262656 2306 12-13 0.19321086197752635 2306 14-15 0.41058557207948354 2306 16-17 0.43993263966388696 2306 18-19 0.4230009967401145 2306 20-21 0.31653229069716105 2306 22-23 0.4318534193855754 2306 24-25 0.30822604441230794 2306 26-27 0.37300684502555725 2306 28-29 0.43951509056297056 2306 30-31 0.4391912232498143 2306 32-33 0.39142499429301125 2306 34-35 0.364672512123839 2306 36-37 0.46124797618089985 2306 38-39 0.41853534468021536 2306 40-41 0.5230616177854728 2306 42-43 0.4952805896964989 2306 44-45 0.463702756177959 2306 46-47 0.444390411902738 2306 48-49 0.5065422433290436 2306 50-51 0.4331783016641424 2306 52-53 0.39479997393296884 2306 54-55 0.48437284254394797 2306 56-57 0.5684894546328252 2306 58-59 0.5576570174862567 2306 60-61 0.5800914293733541 2306 62-63 0.4664259050771449 2306 64-65 0.3033787465339586 2306 66-67 0.44159867018615273 2306 68-69 0.39475117392965586 2306 70-71 0.40314558839848047 2306 72-73 0.4199614798336242 2306 74-75 0.3174890371250143 2306 76-77 0.11322165095844383 2306 78-79 0.5058199159589307 2306 80-81 0.5025962551172469 2306 82-83 0.4978475510104232 2306 84-85 0.49424808919382457 2306 86-87 0.5097318910072488 2306 88-89 0.6582435901220904 2306 90-91 0.6784518425280623 2306 92-93 0.50610115455477 2306 94-95 0.48006817858833273 2306 96-97 0.48296690723542923 2306 98-99 0.47737245710951015 2306 100-101 0.3989316820083033 2307 1 0.14805529977211052 2307 2 0.15703650884143627 2307 3 0.10176722251518555 2307 4 0.09500274402871156 2307 5 0.17782018601029392 2307 6 0.14027521933527254 2307 7 0.1532803569073664 2307 8 0.1897823890985677 2307 9 0.1859478335900846 2307 10-11 0.18283778551985108 2307 12-13 0.1381375508794207 2307 14-15 0.31495823418475766 2307 16-17 0.27498048453167456 2307 18-19 0.313174340718092 2307 20-21 0.2891212377795611 2307 22-23 0.3124235992979294 2307 24-25 0.2964095197977059 2307 26-27 0.31845194751065975 2307 28-29 0.4527411663720713 2307 30-31 0.3383191088574975 2307 32-33 0.4169217111494419 2307 34-35 0.3623881112963545 2307 36-37 0.2611046704640003 2307 38-39 0.2821951365853863 2307 40-41 0.3077692101107701 2307 42-43 0.33092676846081304 2307 44-45 0.34567702630082664 2307 46-47 0.3926567536835961 2307 48-49 0.4132613715318598 2307 50-51 0.2991305671557285 2307 52-53 0.25239209028554654 2307 54-55 0.4607290612940602 2307 56-57 0.39410314101734656 2307 58-59 0.40320571721171916 2307 60-61 0.18864805046977295 2307 62-63 0.1623820132019702 2307 64-65 0.10468041717717824 2307 66-67 0.1731491394603779 2307 68-69 0.14864533037288652 2307 70-71 0.21918123124367384 2307 72-73 0.17303520653636184 2307 74-75 0.15880357479620955 2307 76-77 -0.20353412946935023 2307 78-79 0.11590100634742129 2307 80-81 0.32584633266321106 2307 82-83 0.14547770240409363 2307 84-85 0.17018788654668526 2307 86-87 0.10532883733898046 2307 88-89 0.30717426297799477 2307 90-91 0.36437075707819844 2307 92-93 0.1881303764224853 2307 94-95 0.26624064425013927 2307 96-97 0.08070090280092046 2307 98-99 0.07599944227215616 2307 100-101 -0.0665963552532709 2201 1 0.14477651949258785 2201 2 0.15631365748524928 2201 3 0.09326498993293342 2201 4 0.1084463236738813 2201 5 0.18542039228675122 2201 6 0.14225437473852764 2201 7 0.1619084798193029 2201 8 0.10831046515469467 2201 9 0.13209986959974884 2201 10-11 0.12669355765645207 2201 12-13 0.16272004205583812 2201 14-15 0.31685962869957507 2201 16-17 0.3040427393357774 2201 18-19 0.2781163531889419 2201 20-21 0.32276926228043834 2201 22-23 0.257093066569567 2201 24-25 0.3158125293130425 2201 26-27 0.2730515885369442 2201 28-29 0.36233622752028793 2201 30-31 0.32486344270097334 2201 32-33 0.2821906399258367 2201 34-35 0.2826148403866071 2201 36-37 0.29122092104276476 2201 38-39 0.21090824283439957 2201 40-41 0.34372077638401066 2201 42-43 0.4173799379513028 2201 44-45 0.294515430250577 2201 46-47 0.4030267204963778 2201 48-49 0.5184809942732898 2201 50-51 0.2928499999786425 2201 52-53 0.36522123328170863 2201 54-55 0.48079453292965724 2201 56-57 0.5050468315638383 2201 58-59 0.46229116945345083 2201 60-61 0.34252086328773856 2201 62-63 0.37816297685127864 2201 64-65 0.3908297863565906 2201 66-67 0.46499125548534437 2201 68-69 0.26163796617849044 2201 70-71 0.4199436865019166 2201 72-73 0.36741983935593936 2201 74-75 0.3056111935784571 2201 76-77 0.16885371117099268 2201 78-79 0.40083916846297285 2201 80-81 0.4133525015492907 2201 82-83 0.29103612175526905 2201 84-85 0.2854039205906176 2201 86-87 0.3139889396870359 2201 88-89 0.5401770323919592 2201 90-91 0.6032546323031127 2201 92-93 0.45505192551988927 2201 94-95 0.5043608598977656 2201 96-97 0.5238538811252553 2201 98-99 0.31607624317101113 2201 100-101 0.3488786853643404 1101 1 0.14127552024740453 1101 2 0.17071738420118976 1101 3 0.1354683762216169 1101 4 0.10439282669567973 1101 5 0.1748795587465679 1101 6 0.17727402402752546 1101 7 0.22329430244251824 1101 8 0.12396720607030431 1101 9 0.09787002294398661 1101 10-11 0.15497558416114998 1101 12-13 0.15299829827800693 1101 14-15 0.2975056647737233 1101 16-17 0.27492887920984543 1101 18-19 0.2688419900969379 1101 20-21 0.36405596142785157 1101 22-23 0.3476663629135146 1101 24-25 0.3322135590801665 1101 26-27 0.31347835425003723 1101 28-29 0.23601925656299017 1101 30-31 0.32712179697328736 1101 32-33 0.3722762659928449 1101 34-35 0.3093717990084812 1101 36-37 0.33314924578098015 1101 38-39 0.3845460113894532 1101 40-41 0.35757873349118796 1101 42-43 0.39495082546846305 1101 44-45 0.4385801639878295 1101 46-47 0.4781296845438092 1101 48-49 0.6255748435329096 1101 50-51 0.46135880068177926 1101 52-53 0.45534493261656195 1101 54-55 0.5869993990890663 1101 56-57 0.3859316448101069 1101 58-59 0.5215632528986589 1101 60-61 0.5087831037246247 1101 62-63 0.4861908480664354 1101 64-65 0.4821075568873354 1101 66-67 0.36450901520044 1101 68-69 0.4344924227613447 1101 70-71 0.5357533062314985 1101 72-73 0.40950639374577946 1101 74-75 0.5240695887577687 1101 76-77 0.2392629774286732 1101 78-79 0.5055789657770475 1101 80-81 0.5608484995026757 1101 82-83 0.545320116355029 1101 84-85 0.4418230735498483 1101 86-87 0.43789906271197765 1101 88-89 0.5336454485186906 1101 90-91 0.6106096594309136 1101 92-93 0.4828840383978701 1101 94-95 0.38952713480290413 1101 96-97 0.5503249340434522 1101 98-99 0.6163393682375045 1101 100-101 0.6215406701008348 2304 1 0.12673494300486965 2304 2 0.16158852772332466 2304 3 0.12942176245213233 2304 4 0.1043828839772658 2304 5 0.12853582773139038 2304 6 0.15507913867715928 2304 7 0.1530334343308013 2304 8 0.12692004934194046 2304 9 0.18152791158323822 2304 10-11 0.14346025911783045 2304 12-13 0.12954695592456034 2304 14-15 0.21488369211532898 2304 16-17 0.18193874065733695 2304 18-19 0.26327752612185407 2304 20-21 0.2562534059474828 2304 22-23 0.21878726175832242 2304 24-25 0.21507435871680514 2304 26-27 0.2605341712302476 2304 28-29 0.26548702428725335 2304 30-31 0.21684112700121716 2304 32-33 0.3324913428428502 2304 34-35 0.4039503228519834 2304 36-37 0.33524989721636445 2304 38-39 0.32372899660276033 2304 40-41 0.282600693930128 2304 42-43 0.34606384795111467 2304 44-45 0.4230871751893872 2304 46-47 0.35337003895416785 2304 48-49 0.3216361004769652 2304 50-51 0.2837876136998858 2304 52-53 0.21801476577486056 2304 54-55 0.42048127279290526 2304 56-57 0.38177479597747066 2304 58-59 0.3637068194122719 2304 60-61 0.3732351763635293 2304 62-63 0.32759951800161247 2304 64-65 0.3020568864887494 2304 66-67 0.356500587809947 2304 68-69 0.3174077632519001 2304 70-71 0.41458831359199877 2304 72-73 0.3028973967672144 2304 74-75 0.30506233893096635 2304 76-77 0.2941140729721461 2304 78-79 0.4374173081270314 2304 80-81 0.4689609940125692 2304 82-83 0.34226754329478837 2304 84-85 0.4667231708443964 2304 86-87 0.4614803088429653 2304 88-89 0.5629952004982997 2304 90-91 0.6844563905532866 2304 92-93 0.48493367936486464 2304 94-95 0.37543448422980674 2304 96-97 0.3242057586031777 2304 98-99 0.27468919111301204 2304 100-101 0.4173284014203418 2202 1 0.14765077725098052 2202 2 0.1486531127404831 2202 3 0.10419819733089497 2202 4 0.043538038223417175 2202 5 0.1868294514055293 2202 6 0.10377423298695021 2202 7 0.13311476241736386 2202 8 0.1668309873636602 2202 9 0.12521027903827076 2202 10-11 0.11552950683432783 2202 12-13 0.10015433167519916 2202 14-15 0.2230245623263727 2202 16-17 0.15896857517181928 2202 18-19 0.1600719398283701 2202 20-21 0.20058173799616696 2202 22-23 0.2597455517933014 2202 24-25 0.1764658819137992 2202 26-27 0.16309305178712208 2202 28-29 0.2645821797608434 2202 30-31 0.1990919885473943 2202 32-33 0.3173225520864875 2202 34-35 0.32180158300304385 2202 36-37 0.2778659289965759 2202 38-39 0.2321971919482806 2202 40-41 0.30077182877861475 2202 42-43 0.38556904271307957 2202 44-45 0.2526918443379671 2202 46-47 0.40802985173885276 2202 48-49 0.43618917047310646 2202 50-51 0.3405287379279258 2202 52-53 0.3882119384055187 2202 54-55 0.3921691569520078 2202 56-57 0.4529717834524192 2202 58-59 0.5156445701656409 2202 60-61 0.47379306196851445 2202 62-63 0.3344682878499441 2202 64-65 0.41018899300944867 2202 66-67 0.3320015874427611 2202 68-69 0.39758467277420806 2202 70-71 0.48500560567929085 2202 72-73 0.28575254986914445 2202 74-75 0.43433197245788335 2202 76-77 0.12750916821811842 2202 78-79 0.5200122290353804 2202 80-81 0.6280771693404645 2202 82-83 0.4994684492055228 2202 84-85 0.4361791745183794 2202 86-87 0.45801488043112926 2202 88-89 0.5479970676584358 2202 90-91 0.6178669067736635 2202 92-93 0.48862093056187916 2202 94-95 0.4719704990963507 2202 96-97 0.3575216945809174 2202 98-99 0.30596405840941543 2202 100-101 0.33838738027618476 1102 1 0.11730199074685288 1102 2 0.10948685646617662 1102 3 0.09170889103500457 1102 4 0.06399438006904745 1102 5 0.1162857158732109 1102 6 0.0712404235261559 1102 7 0.029022987837393543 1102 8 0.05584389790969624 1102 9 0.11012085689241502 1102 10-11 0.06910707966903118 1102 12-13 0.05515905751502004 1102 14-15 0.13782981939831984 1102 16-17 0.15648235676209055 1102 18-19 0.17899428202256473 1102 20-21 0.11147470839078721 1102 22-23 0.09964100479054139 1102 24-25 0.16211860102535525 1102 26-27 0.17412682111262967 1102 28-29 0.23455899530411983 1102 30-31 0.20728754213595835 1102 32-33 0.13250804581923603 1102 34-35 0.1463319097332132 1102 36-37 0.12229666959027696 1102 38-39 0.08777704562442068 1102 40-41 0.22619702780986728 1102 42-43 0.14104769037803777 1102 44-45 0.11628537727747101 1102 46-47 0.24366355178579369 1102 48-49 0.2214131289985204 1102 50-51 0.17808441208144643 1102 52-53 0.12088506432257162 1102 54-55 0.27663828279096236 1102 56-57 0.25345799896951604 1102 58-59 0.27640372010746006 1102 60-61 0.15783180011504072 1102 62-63 0.24078728167634011 1102 64-65 0.1849521044127158 1102 66-67 0.13288807110697576 1102 68-69 0.05716131418095216 1102 70-71 0.03652648000666403 1102 72-73 0.06491318720306083 1102 74-75 0.054828775013234576 1102 76-77 -0.17933198209540535 1102 78-79 0.251752299890434 1102 80-81 0.36149261730452054 1102 82-83 0.27741183024561167 1102 84-85 0.23172169876045956 1102 86-87 0.2811386641691769 1102 88-89 0.41418746223532565 1102 90-91 0.42885338154302843 1102 92-93 0.3251714146079223 1102 94-95 0.2876535257743811 1102 96-97 0.16098081407776732 1102 98-99 0.0674161748408082 1102 100-101 0.12987895222718748 2305 1 0.09798956610926979 2305 2 0.10117530721027634 2305 3 0.08073670388237986 2305 4 0.0728052384632818 2305 5 0.11914583184510263 2305 6 0.07070561689040034 2305 7 0.10407898569771845 2305 8 0.08587690165212081 2305 9 0.0446044207697156 2305 10-11 0.05428642798409555 2305 12-13 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-1.5032289681789592 1213 98-99 -1.58237818784756 1213 100-101 -1.52541549217338 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 9.0 8 31.0 9 148.0 10 244.0 11 184.0 12 150.0 13 228.0 14 564.0 15 1094.0 16 2115.0 17 3649.0 18 4938.0 19 6656.0 20 8792.0 21 11164.0 22 14029.0 23 17371.0 24 21840.0 25 26930.0 26 33781.0 27 42405.0 28 54367.0 29 69501.0 30 81721.0 31 98566.0 32 131695.0 33 185920.0 34 285019.0 35 494200.0 36 982800.0 37 1581395.0 38 455700.0 39 214.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.065276741454774 33.26252224734469 15.777449095536818 17.894751915663715 2 30.117901338842906 25.818097552312764 29.908477028297188 14.155524080547147 3 31.61988729636897 23.737281858700314 25.313984866007424 19.328845978923294 4 27.663358818141393 23.560433952877183 29.2907362553035 19.485470973677923 5 29.65309631785716 21.928977654187836 29.83302363657627 18.584902391378737 6 27.528533250170007 22.190249100146836 31.50802829285618 18.773189356826972 7 30.910053666333148 23.338574355375947 25.088620918175085 20.662751060115824 8 27.285865266750697 26.93647101628182 25.277016169202714 20.500647547764768 9 26.255939464029698 24.16061350277861 28.594474836597062 20.988972196594634 10-11 22.41805031712153 29.2555197122137 30.99722457748713 17.329205393177638 12-13 22.031090955555264 28.98873180260838 30.080748556553228 18.899428685283123 14-15 21.820074846992476 29.015315912349323 30.471919383551853 18.69268985710635 16-17 22.074051069344534 28.420544599914066 30.742810685128973 18.76259364561243 18-19 21.23819145756721 28.361801612155705 31.26325956919665 19.13674736108043 20-21 21.787870715681052 28.72808841300986 30.777165603302276 18.706875268006808 22-23 21.321636325047322 29.822119278731414 30.145795704094496 18.71044869212677 24-25 21.167654231150728 28.93126464562461 30.672734990535837 19.228346132688817 26-27 21.031246886789592 28.89485903383275 30.465563020041497 19.608331059336166 28-29 21.107057620922507 29.703070112746943 30.2856681869962 18.904204079334345 30-31 20.465454734462103 29.630291374837032 30.711078914198836 19.19317497650203 32-33 20.390434485058755 30.05473836038307 30.313475923784278 19.241351230773894 34-35 20.912522577543303 30.09924373351352 30.356974241026375 18.6312594479168 36-37 20.786997933911145 29.79923853580571 30.763045163749453 18.65071836653369 38-39 20.77541692181995 29.117556765852935 31.11037836321808 18.996647949109036 40-41 20.638400081782297 29.358307834120545 29.86338284165769 20.13990924243947 42-43 20.923107677017352 29.55160213410689 30.322334865146676 19.20295532372908 44-45 20.50796259990135 29.221466560762167 30.552690467024775 19.717880372311715 46-47 20.963545939155694 29.32751801044484 30.08835067371653 19.620585376682936 48-49 20.557293566337478 30.052167758133585 30.210743794492906 19.179794881036024 50-51 21.158626592654574 29.358307631281345 29.853361585827347 19.629704190236733 52-53 20.554564857647595 29.247633073073438 29.659123753780904 20.538678315498064 54-55 21.110312534454863 29.465864855269537 29.825484285116126 19.598338325159474 56-57 20.60192496067287 29.221801548650618 30.095414051537063 20.080859439139452 58-59 20.688456603432698 29.51997525203433 30.100103181513028 19.691464963019946 60-61 20.81540373865626 29.69305515960733 29.880471053705598 19.611070048030808 62-63 20.310547443346447 29.757652438241884 29.640886876783306 20.290913241628363 64-65 20.670290684999248 29.57237731682067 29.812527285461677 19.944804712718405 66-67 20.61109397393262 29.84708245312741 29.570662411640036 19.97116116129994 68-69 20.502525854549468 30.058432760299386 29.69753759645056 19.741503788700587 70-71 20.5432819117357 30.58265209708763 29.38526640650954 19.48879958466713 72-73 20.417750491295322 30.514497544389872 29.36218473050675 19.70556723380805 74-75 20.553487699996513 30.03164284787364 29.379537177313253 20.035332274816593 76-77 20.661362024142228 29.77383436096836 29.434992638137686 20.12981097675172 78-79 20.443446804362658 29.60634253593129 29.82508204356161 20.125128616144437 80-81 20.40481450793789 29.805695403956268 29.606475282074186 20.18301480603166 82-83 20.087860138475875 29.904424377809857 29.50956487162464 20.498150612089628 84-85 20.095920264479762 29.976983208376577 29.605610838402935 20.321485688740722 86-87 20.148463835918356 29.75516779058525 29.987710514041925 20.108657859454464 88-89 20.39360333119126 30.115852782125046 29.562871046855925 19.92767283982777 90-91 20.59673691080735 29.740548039033687 29.188962969814508 20.473752080344457 92-93 20.265934414270127 30.109286438499716 29.19774097232311 20.427038174907054 94-95 19.769493442420487 30.450656191295415 29.19988429712671 20.579966069157386 96-97 19.95210378927747 30.623872625716718 29.062317069102008 20.361706515903805 98-99 20.136130960970057 30.239474447089165 28.94085601460476 20.68353857733602 100-101 20.157081419810122 30.1167293846209 28.83461602084886 20.891573174720122 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 67.0 1 46.5 2 35.5 3 82.0 4 107.5 5 81.5 6 64.5 7 62.0 8 59.0 9 67.0 10 79.5 11 78.0 12 88.5 13 113.5 14 151.5 15 186.0 16 219.0 17 295.0 18 413.5 19 596.5 20 919.5 21 1462.0 22 2422.0 23 3847.0 24 5904.0 25 8860.5 26 13250.5 27 19298.0 28 26172.5 29 33607.5 30 41435.0 31 50178.5 32 64351.5 33 87559.5 34 116444.5 35 153574.5 36 200688.0 37 257155.0 38 309896.5 39 326089.0 40 331246.0 41 317796.5 42 282886.0 43 269591.0 44 253380.5 45 238226.5 46 240584.0 47 222792.5 48 177145.0 49 133042.5 50 101157.5 51 81352.0 52 65201.5 53 49352.5 54 38051.0 55 28990.0 56 19866.5 57 12955.5 58 8508.0 59 5822.0 60 4157.0 61 2995.0 62 2055.5 63 1361.5 64 895.0 65 605.5 66 414.5 67 275.5 68 199.5 69 149.0 70 109.5 71 73.5 72 51.5 73 39.5 74 26.0 75 21.5 76 19.5 77 14.5 78 8.5 79 6.5 80 6.5 81 5.0 82 3.5 83 1.5 84 0.5 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18285102936271772 2 0.0 3 0.0 4 0.009117645784875537 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.002273997167249243 12-13 0.0 14-15 0.0 16-17 2.5988539054277066E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0021115687981600115 40-41 0.006150620909512238 42-43 0.00271796804275981 44-45 0.0011369985836246215 46-47 0.006659563132658497 48-49 0.008489589424397174 50-51 0.00533847906406608 52-53 0.006594591785022805 54-55 0.018906662161986564 56-57 0.02196031550086412 58-59 0.027786079672197893 60-61 0.026335052908334093 62-63 0.010319615716135851 64-65 0.015625609106384084 66-67 0.0233030566853351 68-69 0.027277137449051633 70-71 0.029529477500422317 72-73 0.022783285904249558 74-75 0.033070415946567566 76-77 0.030330790787929187 78-79 0.021007402402207293 80-81 0.009020188763421998 82-83 0.001765054944102984 84-85 0.007709933252768863 86-87 0.02769945120868364 88-89 0.02541462548349511 90-91 0.021592144530928527 92-93 0.04486271554244578 94-95 0.04656279913891307 96-97 0.03478132810097414 98-99 0.041971490572657456 100-101 0.041484205465389765 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4617420.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.96651847895324 #Duplication Level Percentage of deduplicated Percentage of total 1 90.0032506804241 44.07145837608848 2 4.7499639226046915 4.651783923811677 3 1.5093944444803271 2.217293728730259 4 0.7252150980957891 1.4204503400849338 5 0.46277484184072654 1.13302364222943 6 0.3708830420062013 1.089651079795623 7 0.2175511351721188 0.7456905176365966 8 0.18485460042839263 0.7241348966237124 9 0.14676231130914513 0.6467795485859829 >10 1.3342684425952092 13.147806675080167 >50 0.16047096519526682 5.513602302490152 >100 0.1060917704183766 9.972070202502884 >500 0.01774656931440725 6.2070223739799895 >1k 0.010728161045609383 7.841641287919863 >5k 0.0 0.0 >10k+ 4.401506954535851E-5 0.6175911044400648 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 28655 0.6205846555002577 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.312559827782614E-4 0.0 0.0 2 0.0011261700256853394 0.0 0.0 3 0.001775883502042266 0.0 2.165711587856422E-5 4 0.002533882557792014 0.0 2.165711587856422E-5 5 0.0030969675706346836 0.0 2.165711587856422E-5 6 0.005349307622005362 0.0 2.165711587856422E-5 7 0.007038562660533372 0.0 4.331423175712844E-5 8 0.008662846351425687 0.0 4.331423175712844E-5 9 0.009832330608868156 0.0 6.497134763569266E-5 10-11 0.013221669243863456 0.0 6.497134763569266E-5 12-13 0.019209861784286464 0.0 6.497134763569266E-5 14-15 0.02749370860783728 0.0 6.497134763569266E-5 16-17 0.03782415288191241 0.0 6.497134763569266E-5 18-19 0.0509050508725652 0.0 6.497134763569266E-5 20-21 0.06479809070866414 0.0 6.497134763569266E-5 22-23 0.08324995343720086 0.0 6.497134763569266E-5 24-25 0.10375924217420118 0.0 6.497134763569266E-5 26-27 0.12501570140901197 0.0 6.497134763569266E-5 28-29 0.14760407327035446 0.0 8.662846351425688E-5 30-31 0.17226069969809982 0.0 1.082855793928211E-4 32-33 0.19580198465809912 0.0 1.082855793928211E-4 34-35 0.2211191531201407 0.0 1.1911413733210321E-4 36-37 0.2511034300540128 0.0 1.7325692702851376E-4 38-39 0.2843904171593661 0.0 1.7325692702851376E-4 40-41 0.3181322036981691 0.0 1.7325692702851376E-4 42-43 0.3572666120907346 0.0 1.8408548496779586E-4 44-45 0.3993572168007242 0.0 1.9491404290707798E-4 46-47 0.44260647721021695 0.0 1.9491404290707798E-4 48-49 0.49163818755928634 0.0 1.9491404290707798E-4 50-51 0.5434311801828726 0.0 1.9491404290707798E-4 52-53 0.5962745429265695 0.0 1.9491404290707798E-4 54-55 0.6545971559875428 0.0 1.9491404290707798E-4 56-57 0.7140026248424445 0.0 1.9491404290707798E-4 58-59 0.777934430915966 0.0 2.057426008463601E-4 60-61 0.8451256329292116 0.0 2.165711587856422E-4 62-63 0.9160093731997523 0.0 2.165711587856422E-4 64-65 0.9890371679422707 0.0 2.165711587856422E-4 66-67 1.0643822740837958 0.0 2.3822827466420641E-4 68-69 1.145997115272165 0.0 2.3822827466420641E-4 70-71 1.2306764383573512 0.0 2.3822827466420641E-4 72-73 1.3186368144981397 0.0 2.5988539054277066E-4 74-75 1.409769958115138 0.0 2.5988539054277066E-4 76-77 1.5061116381009307 0.0 2.8154250642133485E-4 78-79 1.6067305984727402 0.0 2.8154250642133485E-4 80-81 1.7127421806983119 0.0 3.031996222998991E-4 82-83 1.8242113561252822 0.0 3.031996222998991E-4 84-85 1.9430764366247817 0.0 3.1402818023918116E-4 86-87 2.0706585062654037 0.0 3.248567381784633E-4 88-89 2.1998323739231003 0.0 3.248567381784633E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGCGC 25 0.005025412 56.990887 2 TCGCGGC 25 0.005025412 56.990887 2 CGAGCGC 85 1.2732926E-10 50.378277 1 CGACGGG 95 7.2759576E-12 50.08367 1 CGCGGCG 40 5.345634E-4 47.579483 1 CGACGTG 90 1.1803422E-8 42.292873 1 CGGTTCG 45 9.549198E-4 42.292873 1 CGCGTGA 45 9.549198E-4 42.292873 1 CGGGTCG 70 4.263762E-6 40.782413 1 CGACGCC 70 4.263762E-6 40.782413 1 CGAGGCG 150 0.0 38.063587 1 CCGATGG 100 2.989873E-8 38.063583 1 CGTGCGG 50 0.0016030999 38.063583 1 CGGGATG 200 0.0 38.063583 1 GTCCGGC 100 2.989873E-8 38.063583 1 AACGCGG 65 1.3157989E-4 36.5996 1 CGATGCG 105 4.5945853E-8 36.251034 1 CGGGGCG 185 0.0 36.006096 1 AGGCCGC 135 3.0377123E-10 35.24406 1 GTCGCGG 55 0.0025593578 34.60326 1 >>END_MODULE